CN111466177B - Agar gel germination bed, pore-forming device and seed germination method - Google Patents
Agar gel germination bed, pore-forming device and seed germination method Download PDFInfo
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- CN111466177B CN111466177B CN202010342616.2A CN202010342616A CN111466177B CN 111466177 B CN111466177 B CN 111466177B CN 202010342616 A CN202010342616 A CN 202010342616A CN 111466177 B CN111466177 B CN 111466177B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C5/00—Making or covering furrows or holes for sowing, planting or manuring
- A01C5/02—Hand tools for making holes for sowing, planting or manuring
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Health & Medical Sciences (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides an agar gel germination bed, a pore-forming device and a seed germination method, which belong to the technical field of seed germination, wherein the agar gel germination bed takes agar gel as a germination bed matrix, 40-60 germination pores are formed in the agar gel, and the concentration of the agar gel is 0.3-3%; the thickness of the agar gel is 1-1.5 cm, and the depth of the germination holes is 0.8-1.2 cm. The seed germination method comprises the following steps: 1) Injecting the agar solution into a container after hot melting, wherein the thickness of the agar solution is 1-1.5 cm; 2) Vertically inserting the hole making device into the agar solution, and pulling out the agar solution after the agar solution is solidified to obtain an agar gel germination bed; 3) The seeds were placed in germination wells for cultivation. The seed germination method can enable wheat to sprout rapidly, has high germination rate, and can be used for uniform and stable mass production of wheat seedlings.
Description
Technical Field
The invention belongs to the technical field of seed germination, and particularly relates to an agar gel germination bed, a pore-forming device and a seed germination method.
Background
The germination beds used in the traditional germination method mainly comprise a paper germination bed, a sand germination bed and a soil germination bed, and the paper germination bed is mainly selected on the germination of wheat seeds. However, paper beds have been found to have a number of disadvantages during practical use. For example, the paper-made germination bed has poor water retention performance, and water needs to be continuously supplemented in the germination process of seeds in order to ensure that the seeds can sprout normally; if the water is supplemented too much at one time, the water is mixed into a group, and the counting is difficult. Secondly, the paper germination bed has poor adsorptivity, is difficult to dibble, and the seeds are easy to roll in the dibble process, and moreover, the paper germination bed has poor supporting performance and is easy to damage, so that the germination effect is affected.
Disclosure of Invention
In view of the above, the present invention aims to provide an agar gel germination bed, a hole making device and a seed germination method; the seed germination method can enable wheat to sprout rapidly, has high germination rate, and can be used for uniform and stable mass production of wheat seedlings.
The invention provides an agar gel germination bed, which takes agar gel as a germination bed matrix, wherein 40-60 germination holes are formed in the agar gel, and the concentration of the agar gel is 0.3% -3%; the thickness of the agar gel is 1-1.5 cm, and the depth of the germination holes is 0.8-1.2 cm.
Preferably, the diameter of the germination holes is 0.3-0.4 cm.
Preferably, when the seed to be germinated is a bare seed, the concentration of the agar gel is 1.5% -2.1%.
Preferably, when the seed to be germinated is a bare seed, the concentration of the agar gel is 0.3% -1.5%.
The invention provides a hole making device, which comprises a bottom plate and a plurality of hole making columns fixedly connected to the bottom plate, wherein the hole making columns are vertical to the bottom plate; the diameter of the hole-making column is 0.3-0.4 cm, and the height of the hole-making column is 0.8-1.2 cm; the distance between two adjacent hole-making columns is 1.0-1.2 cm.
The invention provides a seed germination method, which comprises the following steps:
1) Injecting the agar solution into a container after hot melting, wherein the thickness of the agar solution is 1-1.5 cm;
2) Vertically inserting the hole making device into the agar solution, and pulling out the agar solution after the agar solution is solidified to obtain an agar gel germination bed;
3) The seeds were placed in germination wells for cultivation.
Preferably, the seed is a wheat seed.
Preferably, the temperature of the culture in the step 3) is 27-29 ℃, and the humidity of the culture is more than or equal to 40%.
Preferably, no water replenishment is performed during the cultivation.
Preferably, one seed is placed in each germination hole in step 3).
The invention has the beneficial effects that: the agar gel germination bed provided by the invention takes the agar gel as a matrix, so that the problem that the paper germination bed needs to be subjected to water supply is solved, and the sowing and statistics are easier compared with the paper germination bed; the wheat seeds can be fixed in the germination holes without water supplementing, so that the problem of seed accumulation caused by water adding can be avoided; the method can enable the wheat to sprout rapidly, has high germination rate, and can carry out uniform and stable batch production of wheat seedlings. The preparation method of the agar gel germination bed provided by the invention is simple, is easy to operate, can greatly reduce labor and has low cost.
Drawings
FIG. 1 is a schematic diagram of a wheat agar gel germination puncher;
FIG. 2 is a schematic diagram of the structure of an agar germination bed.
Detailed Description
The invention provides an agar gel germination bed, which takes agar gel as a germination bed matrix, wherein 40-60 bud holes are arranged on the agar gel, and the concentration of the agar gel is 0.3-3%; the thickness of the agar gel is 1-1.5 cm, and the depth of the germination holes is 0.8-1.2 cm.
In the present invention, the diameter of the germination holes is preferably 0.3 to 0.4cm. In the invention, the agar gel germination bed is preferably fixed in a container, the shape and the material of the container are not particularly required, and the agar gel germination bed is prepared by adopting a container conventional in the art. In the practice of the invention, the vessel may be selected as a petri dish; the diameter of the dish is preferably 10cm.
In the present invention, when the seed to be germinated is a bare seed, the concentration of the agar gel is preferably 1.5% to 3%, more preferably 1.5% to 2.1%. When the seed to be germinated is a coated seed, the concentration of the agar gel is preferably 0.3% to 1.5%, more preferably 0.4% to 0.9%. In the present invention, the concentration of the agar gel specifically refers to the gram number of the agar powder used per 100ml of the buffer; if the concentration of the agar gel is 0.3%, the concentration means that 100ml of buffer solution contains 0.3g of agar powder; in the present invention, the buffer is preferably tap water and an agar gel melt buffer.
The invention provides a hole making device, which comprises a bottom plate and a plurality of hole making columns fixedly connected to the bottom plate, wherein the hole making columns are vertical to the bottom plate; the diameter of the hole-making column is 0.3-0.4 cm, and the height of the hole-making column is 0.8-1.2 cm; the distance between two adjacent hole-making columns is 1.0-1.2 cm.
The shape and the material of the bottom plate are not particularly limited, and the shape and the material which are conventional in the art can be adopted. In the present invention, the shape of the bottom plate preferably conforms to the shape of the agarose gel germination bed. In the present invention, wood material is preferably used for the bottom plate and the hole-making columns.
The invention provides a seed germination method, which comprises the following steps: 1) Injecting the agar solution into a container after hot melting, wherein the thickness of the agar solution is 1-1.5 cm; 2) Vertically inserting the hole making device into the agar solution, and pulling out the agar solution after the agar solution is solidified to obtain an agar gel germination bed; 3) The seeds were placed in germination wells for cultivation.
In the present invention, the agar solution is injected into the container after hot melting, and the thickness of the agar solution is 1 to 1.5cm. In the present invention, the temperature of the hot melting of the agar solution is preferably 110 to 125℃and in the present invention, the thickness of the agar solution is preferably 1.1 to 1.4cm.
In the invention, the pore-forming device is vertically inserted into the agar solution, and the agar gel germination bed is obtained after the agar solution is solidified and then pulled out. In the present invention, the hole making device is preferably inserted immediately after the agar solution is injected into the container.
In the present invention, seeds are placed in germination holes for cultivation. In the present invention, the seeds are preferably wheat seeds; preferably, one seed is placed in each germination hole. In the present invention, the temperature of the culture is 27 to 29 ℃, more preferably 28 ℃, and the humidity of the culture is preferably not less than 40%. In the invention, the culture is preferably carried out in a constant temperature incubator, and the illumination condition during the culture is that 12h illumination and 12h darkness are alternately carried out. The present invention preferably does not make up water during the cultivation.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Wheat seeds in the following examples are provided by inner Mongolia agriculture and animal husbandry academy of agriculture and animal husbandry crops.
The agar powder is produced by Shanghai Gaifeng Co., ltd, and has gel strength of 900g/cm 2 The total ash content is less than or equal to 6.5 percent, the heavy metal content is less than 40ppm, and the lead content is less than 10ppm.
The seed cultivation temperature is 28 ℃, the relative humidity is more than 40 percent, and the seeds are cultivated in an artificial climate incubator (RZH 850A) with alternating illumination and darkness for 12 hours, and the radicle and the seed are equal in length and serve as germination standards.
The germination of naked seeds is strictly carried out according to national standard GB/T17317-1998, and the coating of seeds is carried out according to GB 15671-1995.
The germination vigor is the percentage of the number of germinated seeds to the number of seeds to be measured when the number of germinated seeds reaches the highest peak in the germination process.
The germination percentage is the percentage of the number of normal germinated seeds to the number of seeds tested within a predetermined number of days (at the time of initial counting) at the initial stage of the germination test.
Analysis of variance was performed using SPSS16.0 and the results are the average of four replicates.
The specification of the culture dish is that the diameter of the bottom of the dish is 10cm, the height of the dish is 2.0cm, and the thickness of the bottom of the dish is 0.2cm.
Example 1
1. Preparation of a puncher: on a square wood board with the side length of 8cm, 7 columns with the diameter of 0.3cm and the length of more than 1.0cm are fixed, and each column is fixed with 7 columns except 8 holes in the middle, wherein the maximum row spacing between two adjacent columns and the maximum column spacing between two adjacent columns are 1.2cm.
2. And (3) dissolving agar powder by adopting an SX-500 autoclave to obtain agar solution with the required concentration, injecting the obtained agar solution into the culture dish in the step (2) to enable the liquid agar to be thicker than 1.0cm, immediately inserting the cylindrical object on the puncher downwards into the culture dish, and after solidification, slightly pulling out the puncher vertically, thus leaving 50 sowing holes on the agar bed.
Agar gel germination beds with agar gel concentrations of 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, 1.8%, 2.1%, 2.4%, 2.7%, 3.0% were prepared according to the above method, respectively; 3 parts of each concentration were prepared, and then seed No. 2, no. 4, ning Chun and No. 33 bare seed of Avena sativa and No. 2 seed of Avena sativa were planted in the wells on the agar bed. The following operations are performed:
(1) 2 pieces of filter paper are spread in a culture dish, the filter paper is soaked by distilled water, a paper bed is obtained as a control, and the quantity and the interval of the seeds to be planted are the same as those of the agar bed.
The germination percentage, germination potential and germination index of the seeds in the germination bed were counted, and specific data are shown in tables 1, 2 and 3.
TABLE 1 germination percentage of naked and coated wheat seeds of Nongmai No. 2 on agar and paper beds
As shown in Table 1, the naked wheat seed and the coated wheat seed of the nong wheat No. 2 have obvious difference in germination rate between the naked wheat seed and the coated wheat seed on an agar bed and the germination rate of the paper bed, and the concentration of the agar germination bed has obvious influence on the germination rate of seeds. On the paper bed, the germination rate of the bare seeds is 92 percent which is obviously higher than that of the coated seeds by 79 percent. The germination rates of the wheat bare seeds and the coating seeds with different agar concentrations are also different on the agar bed. The germination rate of the bare wheat seeds and the coated seeds reaches the maximum value from the fourth day when the agar concentration is 0.60%, and the germination rate is 97% and 95% respectively.
TABLE 2 comparison of germination vigor of naked wheat varieties on agar and paper beds
As can be seen from Table 2, the germination vigor of different wheat varieties was significantly different between the agar bed and the paper bed, and the concentration of the agar bed had an effect on the germination vigor of the wheat seeds. The wheat variety has maximum germination potential when the concentration of the agar bed is 0.3%, wherein the maximum germination potential of Ningchun 50 is 99%, and the minimum germination potential of Long Mai is 88%.
TABLE 3 comparison of germination index of naked wheat varieties on agar and paper beds
As can be seen from Table 3, there was a significant difference in germination vigor between the different wheat varieties on the agar bed and the paper bed, and the concentration of the agar bed also had an effect on the germination index of the wheat seeds.
As can be seen from the above embodiments, the agar gel germination bed provided by the invention uses agar gel as a matrix, so that the problem that the paper germination bed needs to be supplemented with water is solved, and compared with the paper germination bed, the wheat germination rate is high, and the sowing and statistics are easier.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (5)
1. A seed germination method comprising the steps of:
1) Injecting the agar solution into a container after hot melting, wherein the thickness of the agar solution is 1-1.5 cm;
2) Vertically inserting the pore-forming device into the agar solution, and pulling out the agar solution after the agar solution is solidified to obtain an agar gel germination bed;
3) Placing seeds into germination holes for culture;
the agar gel germination bed takes agar gel as a germination bed matrix, and 40-60 germination holes are formed in the agar gel; the depth of the germination holes is 0.8-1.2 cm;
the hole making device comprises a bottom plate and a plurality of hole making columns fixedly connected to the bottom plate, and the hole making columns are perpendicular to the bottom plate; the diameter of the hole-making column is 0.3-0.4 cm, and the height of the hole-making column is 0.8-1.2 cm; the distance between two adjacent hole-making columns is 1.0-1.2 cm;
when the seeds to be germinated are bare seeds or coated seeds, the concentration of the agar gel is 0.6%;
the seeds are wheat seeds.
2. The germination method according to claim 1, characterized in that the germination holes have a diameter of 0.3-0.4 cm.
3. The germination method according to claim 1, characterized in that the temperature of the culture in step 3) is 27-29 ℃, the humidity of the culture being equal to or higher than 40%.
4. The germination method according to claim 1, characterized in that no water replenishment is carried out during the cultivation.
5. The germination method according to claim 1, characterized in that in step 3) one seed is placed in each germination opening.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06209662A (en) * | 1993-01-14 | 1994-08-02 | Iwaki Glass Kk | Artificial soil for sterile plant |
| CN102726147A (en) * | 2012-07-12 | 2012-10-17 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
| KR20150076917A (en) * | 2013-12-27 | 2015-07-07 | 전남과학대학교 산학협력단 | Production method of tea-plant |
| CN106612746A (en) * | 2016-12-16 | 2017-05-10 | 沈阳农业大学 | Seed germination potential measuring method |
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2020
- 2020-04-22 CN CN202010342616.2A patent/CN111466177B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06209662A (en) * | 1993-01-14 | 1994-08-02 | Iwaki Glass Kk | Artificial soil for sterile plant |
| CN102726147A (en) * | 2012-07-12 | 2012-10-17 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
| KR20150076917A (en) * | 2013-12-27 | 2015-07-07 | 전남과학대학교 산학협력단 | Production method of tea-plant |
| CN106612746A (en) * | 2016-12-16 | 2017-05-10 | 沈阳农业大学 | Seed germination potential measuring method |
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