CN212660529U - Alfalfa agar gel germinating bed - Google Patents

Abstract

The utility model provides an alfalfa agar gel germinating bed, which belongs to the technical field of alfalfa seed germination, and the alfalfa agar gel germinating bed takes agar gel as germinating bed matrix, a plurality of germinating holes are arranged on the agar gel, and the concentration of the agar gel is 1% -6%; the thickness of the agar gel is 0.8-1 cm, and the depth of the germination holes is 0.5-0.6 cm. The utility model discloses in the alfalfa seed germination bed can make the alfalfa sprout fast, and the germination percentage is high, can carry out even stable alfalfa seedling batch production.

Description

Alfalfa agar gel germinating bed

Technical Field

The utility model belongs to the technical field of the seed germination, especially, relate to an alfalfa agar gel germinating bed.

Background

Alfalfa is a popular name for Medicago plants, commonly known as burclover, and is a perennial flowering plant. Alfalfa is a wide variety of species, mostly a wild herb.

The alfalfa has high protein content, is used as high-quality pasture, and has gradually increased demand along with the development of animal husbandry; the high-quality large-scale planting of the alfalfa is a prerequisite for guaranteeing the alfalfa supply. But the alfalfa seeds have serious mildew phenomenon in the germination process, and the uniformity of the germination of the seeds is poor.

The germination beds used in the traditional germination method mainly comprise three types, namely a paper germination bed, a sand germination bed and a soil germination bed, and the paper germination bed is mainly selected for alfalfa seed germination. However, in practice, paper beds have been found to suffer from a number of disadvantages. For example, a paper germination bed has poor water retention performance, and water needs to be constantly supplemented in the germination process of seeds in order to ensure that the seeds can normally germinate; if the water is supplemented too much at one time, the mixture is mixed into a whole, and the counting is difficult. Secondly, the paper germinating bed has poor adsorptivity and difficult dibbling, seeds are easy to roll in the dibbling process, and moreover, the paper germinating bed has poor supporting performance and is easy to damage, thus influencing the germinating effect.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present invention aims to provide an alfalfa agar gel germination bed; the seed germination bed can enable the alfalfa to germinate rapidly, the germination rate is high, and uniform and stable batch production of alfalfa seedlings can be performed.

The utility model provides an agar gel germinating bed, which takes agar gel as germinating bed matrix, a plurality of germinating holes are arranged on the agar gel, and the concentration of the agar gel is 1% -6%; the thickness of the agar gel is 0.8-1 cm, and the depth of the germination holes is 0.5-0.6 cm.

Preferably, the diameter of the germinating hole is 0.2-0.3 cm.

Preferably, when the seeds to be germinated are naked seeds, the concentration of the agar gel is 3-6%.

Preferably, when the seed to be germinated is a coated seed, the concentration of the agar gel is 1% to 3%.

Preferably, the number of the germination holes is 100.

The utility model has the advantages that: the alfalfa agar gel germinating bed provided by the utility model takes agar gel as a substrate, so that seeding and statistics are easier; alfalfa agar gel germinating bed can make alfalfa germinate fast, and the germination percentage is high, can carry out even stable alfalfa seedling batch production. The preparation method of the alfalfa agar gel germinating bed provided by the utility model is simple and easy to operate, can greatly reduce the labor and has low cost.

Drawings

FIG. 1 is a schematic diagram of the structure of a perforator for germination of alfalfa agar gel;

FIG. 2 is a schematic diagram of the structure of an agar germinating bed.

Detailed Description

The utility model provides an alfalfa agar gel germinating bed, which takes agar gel as germinating bed matrix, a plurality of germinating holes are arranged on the agar gel, and the concentration of the agar gel is 1% -6%; the thickness of the agar gel is 0.8-1 cm, and the depth of the germination holes is 0.5-0.6 cm.

The utility model discloses in, the diameter in germinating hole is 0.2 ~ 0.3cm, and more preferred is 0.25 cm. In the utility model, the agar gel germinating bed is preferably fixed in the container, the utility model has no special requirements for the shape and the material of the container, and the conventional container in the field is adopted. In the specific implementation process of the utility model, the container can be selected as a culture dish; the diameter of the culture dish is preferably 10 cm.

The utility model discloses in, when the alfalfa seed that waits to sprout is the naked kind, the concentration of agar-agar gel is preferred 3 ~ 6%, more preferred 5 ~ 5.9%. When the seeds to be germinated are coated seeds, the concentration of the agar gel is preferably 1% to 3%, more preferably 2.5% to 2.9%. In the present invention, the concentration of the agar gel specifically refers to the gram amount of agar powder used per 100ml of buffer solution; when the concentration of the agar gel is 0.3%, it means that 0.3g of agar powder is contained in 100ml of the buffer solution; in the present invention, the buffer is preferably a tap water and agar gel melting buffer.

The utility model discloses still relate to an alfalfa seed germination method, including following step: 1) injecting the agar solution into a container after hot melting, wherein the thickness of the agar solution is 0.8-1 cm; 2) vertically inserting the hole making device into the agar solution, and pulling out the hole making device after the agar solution is solidified to obtain an agar gel germination bed; 3) and placing the seeds in the germinating holes for culturing.

The utility model discloses in, pour into the container into after the agar solution hot melt, agar solution's thickness is 0.8 ~ 1 cm. In the utility model discloses in, the temperature of agar solution hot melt is preferably 110 ~ 125 ℃, in the utility model discloses, agar solution's thickness is preferably 0.9 cm.

The utility model discloses in, will the hole maker insert perpendicularly in the agar-agar solution, extract after waiting the agar-agar solution to solidify and obtain alfalfa agar gel germinating bed. In the present invention, the perforator is preferably inserted immediately after the agar solution is injected into the container. In the utility model, the hole making device comprises a bottom plate and a plurality of hole making columns fixedly connected to the bottom plate, wherein the hole making columns are perpendicular to the bottom plate; the diameter of the hole making column is 0.2cm, and the height of the hole making column is more than or equal to 0.8 cm; the distance between two adjacent hole making columns is 0.7-1.4 cm.

The utility model discloses it is right the shape and the material of bottom plate do not have special restrictions, adopt the conventional shape in this field and material all can. In the present invention, the shape of the bottom plate is preferably identical to the shape of the agar gel germination bed. In the utility model discloses in, work as bottom plate and the preferred wooden material that adopts of system hole post.

The utility model discloses in, arrange the alfalfa seed in the germination hole and cultivate. In the present invention, preferably, one seed is placed in each germination hole. In the utility model, the temperature of cultivation is 27 ~ 29 ℃, more preferably 28 ℃, the humidity of cultivation is preferred to be more than or equal to 40%. In the utility model, the cultivation is preferably carried out in a constant temperature incubator, and the illumination condition in the cultivation process is 12h illumination-12 h darkness which are carried out alternately. The utility model discloses the preferred moisturizing that does not carry out of in-process of cultivateing.

The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of the present invention.

The alfalfa seeds in the following examples were all stored and provided by the grass center of the academy of sciences in inner Mongolia farming-animal husbandry. Agar powder is produced by Shanghai Gaoyeng company, the gel strength is 900g/cm2, the total ash content is less than or equal to 6.5%, the heavy metal content is less than 40ppm, and the lead content is less than 10 ppm.

The seed cultivation temperature is 28 ℃, the relative humidity is more than 40%, and the seeds are cultivated in an artificial climate incubator (RZH-850A) with 12h alternating illumination and darkness, and the length of radicle and seeds is taken as the standard of germination.

The germination of naked seeds is strictly carried out according to the industrial standard GB/T2930-.

The germination potential is the percentage of the number of the germinated seeds in the seed number of the tested variety when the number of the germinated seeds reaches the maximum peak in the germination process.

The germination percentage is the percentage of the number of normal germinated seeds to the number of test seeds within a predetermined number of days (at the time of initial counting) at the initial stage of the germination test.

Analysis of variance was performed using SPSS16.0 and the results were the average of four replicates.

The culture dish has a diameter of 10cm at the bottom, a height of 1.5cm and a thickness of 0.2cm at the bottom.

Example 1

1. Preparation of a puncher: on a square wood board with the side length of 8cm, 10 rows of cylindrical objects with the section diameter of 0.2cm and the length of more than 0.8cm are fixed, 10 cylindrical objects are fixed in each row, and the row distance between two adjacent rows and the row distance between two adjacent columns are both 0.5 cm.

2. The punch stick is inserted down into the petri dish.

3. Dissolving agar powder in SX-500 autoclave to obtain agar solution with required concentration, injecting the obtained agar solution into the culture dish in step 2 to make the liquid agar thickness be above 0.8cm, solidifying, vertically and lightly inserting a puncher, and leaving 50-100 seeding holes on the agar bed.

3 parts of agar beds with the concentrations of 1%, 2%, 3%, 6%, 9%, 12% and 15% were prepared according to the above method, and then naked species and coated species of alfalfa and yellow clover were inoculated in the holes on the above agar beds. The following operations are carried out:

spreading 2 pieces of filter paper in a culture dish, and wetting the filter paper with distilled water to obtain a paper bed as a control, wherein the number and the spacing of the dibbled seeds are the same as those of the agar bed.

Counting the germination rate, germination vigor and germination index of the seeds in the germination bed, wherein the specific data are shown in tables 1, 2 and 3

TABLE 1 comparison of germination rates of two alfalfa naked and coated species on agar and paper beds

TABLE 2 comparison of germination vigor of two alfalfa naked and coated species on agar and paper beds

TABLE 3 comparison of germination vigor of two alfalfa naked and coated species on agar and paper beds

According to the above embodiment, the utility model provides an alfalfa agar gel germinating bed uses agar gel as the matrix, has not only solved the problem that paper germinating bed needs the moisturizing frequently, and for paper bed seeding more easily, statistics, the alfalfa germination percentage is higher, and the difference is little between the seedling.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (5)

1. The alfalfa agar gel germination bed is characterized in that agar gel is used as a germination bed matrix, 80-120 germination holes are formed in the agar gel, and the concentration of the agar gel is 1% -6%; the thickness of the agar gel is 0.8-1 cm, and the depth of the germination holes is 0.5-0.6 cm.

2. The alfalfa agar gel germination bed according to claim 1, wherein the germination pores have a diameter of 0.2-0.3 cm.

3. The alfalfa agar gel germination bed according to claim 1, wherein the concentration of agar gel is 3% to 6% when the seeds to be germinated are naked seeds.

4. The alfalfa agar gel germination bed according to claim 1, wherein the concentration of agar gel is 1-3% when the seed to be germinated is a coated seed.

5. The alfalfa agar gel germination bed of claim 1, wherein the number of germination holes is 100.

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