CN102550378A - Medium formula of light medium seedling for Camellia oleifera Abel. - Google Patents

Medium formula of light medium seedling for Camellia oleifera Abel. Download PDF

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Publication number
CN102550378A
CN102550378A CN2011104463174A CN201110446317A CN102550378A CN 102550378 A CN102550378 A CN 102550378A CN 2011104463174 A CN2011104463174 A CN 2011104463174A CN 201110446317 A CN201110446317 A CN 201110446317A CN 102550378 A CN102550378 A CN 102550378A
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China
Prior art keywords
seedling
oil tea
light
matrix
mesh bag
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CN2011104463174A
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Chinese (zh)
Inventor
邓先珍
陈慧玲
周席华
张新叶
陈华超
徐春永
李爱华
杜洋文
杨志斌
李振芳
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HUBEI PROV INST OF FORESTRY SCIENCES
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HUBEI PROV INST OF FORESTRY SCIENCES
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Priority to CN2011104463174A priority Critical patent/CN102550378A/en
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    • Y02P60/216

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Abstract

The invention relates to a medium formula of light medium seedling for Camellia oleifera Abel., which is composed of the following components in percentage by volume: 45%-55% of peat, 30%-40% of pearlite and 10%-20% of rice husk. By using the medium formula provided by the invention to culture Camellia oleifera Abel., seedlings cultured with light medium in net-bag container has a survival rate above 94%, a shooting rate above 90%, plant height above 14cm, and ground diameter above 3.28mm.

Description

A kind of matrix formulations that is used for the light substrate seedling of oil tea
 
Technical field
The present invention relates to a kind of matrix formulations that is used for the light substrate seedling of oil tea, belong to the botanical seedling culturing technical field.
 
Background technology
Oil tea Camellia oleirera Abe1. is the distinctive woody oil tree species of China.With olive Olea europaea L., oil palm Elaels gulneensis Jacquin, coconut Cocos nucifera Linnaeus and be called the world's four big woody oil tree species.Oil tea is again one of domestic important economic forest resource that integrates ecological benefits, economic benefit and social benefit, is advancing the mountain area comprehensive exploitation, promotes increasing peasant income to safeguard national food safety, improves the people ' s health situation, is accelerating territory greening process etc. and have a very important role.
It is that employing light soil-less matrix is culturing raw material that light base material mesh bag container is grown seedlings; Adopting the natural fiber material that is prone to degraded to process mesh bag is container material; Utilizing container formation equipment that light matrix mechanization is added installs in the material mesh bag container; Be processed into the nutrition section and place the railway carriage or compartment bed of growing seedlings, utilize cuttage or sowing means to carry out a kind of novel seedling growing process of forest fast breeding.Compare with traditional seedling raising manners, have root growth good, become seedling fast, emerge neat, be convenient to advantages such as transportation, transplanting survival rate height, environmental protection.In recent years, light base material mesh bag container seedling method is applied in the seedling raising process of different seeds more and more, and the cultivation of oil tea improved seeds adopts the light base material mesh bag seedling raising manners that is the basis with the sprout stock grafting will become the inexorable trend that the oil tea kind is grown seedlings.
The inventor is devoted to the cultivation of the light base material mesh bag container grafting of oil tea always; And to disclose with the light base material mesh bag container grafting of breeding oil tea through publishing thesis be test material; Design 7 kinds of different light matrix formulations discussion matrix and select the survival rate that the light base material mesh bag container of oil tea is grown seedlings, influence (the light substrate seedling matrix formulations of the oil tea screening study of taking out tip rate and seedling growth; Chen Hua is superfine, Hubei forestry science and technology, 2011.04).Yet the document does not disclose the concrete composition and the consumption of said 7 kinds of light matrix formulations.The selection of filling substrate and consumption is unusual the key link during the light base material mesh bag container of oil tea is grown seedlings in the mesh bag, and it is directly connected to the survival rate that is grown in the interior oil tea grafting of light base material mesh bag, the growth potential of taking out tip rate, root growth state and acrial part.Therefore, suitable light matrix and the amount ratio thereof of screening seems particularly important.
?
Summary of the invention
The object of the present invention is to provide a kind of cultivation matrix that oil tea grows that helps, a kind of matrix formulations that is used for the light substrate seedling of oil tea especially is provided.
The objective of the invention is to realize like this:
A kind of matrix formulations that is used for the light substrate seedling of oil tea is made up of following components in percentage by volume: peat 45%-55%, perlite 30%-40% and husk 10%-20%.
The described matrix formulations that is used for the light substrate seedling of oil tea is made up of following components in percentage by volume: peat 50%, perlite 40% and husk 10%.
The described matrix formulations that is used for the light substrate seedling of oil tea is made up of following components in percentage by volume: peat 50%, perlite 30% and husk 20%.
The matrix formulations of the light substrate seedling of oil tea that the present invention relates to has obtained following unforeseeable technique effect:
(1) survival rate of utilizing the light base material mesh bag container seedling of matrix formulations cultivation oil tea of the present invention is all more than 94%.
(2) tip rate of taking out of utilizing matrix formulations of the present invention to cultivate the light base material mesh bag container seedling of oil tea reaches more than 90%.
(3) utilize the plant height of the light base material mesh bag container seedling of matrix formulations cultivation oil tea of the present invention, leading thread growth to have significant advantage, plant height to reach more than the 14cm, leading thread reaches more than the 3.28mm.
 
Embodiment
Peat of the present invention is plant organism through plant residue remaining after the corruption of more than one thousand years, has light weight, and water holding is ventilative and be rich in characteristics such as organic nutrient.The used peat of present embodiment is bought from China peat main producing region Qing Yuanxian of Liaoning Province.
Perlite of the present invention is that the acidic lava warp of volcanic eruption sharply cools off the glassy rock that forms, and on horticultural gardening, is mainly used in the matrix preparation of soilless culture, mainly contains chesson, the effect of control fertilizer efficiency and fertile degree.The used pearl rock of present embodiment is bought by Xinyang, Henan, China perlite main producing region.
Husk of the present invention is that shell remaining behind the rice threshing became thoroughly decomposed through 3 years and forms, and has certain fertilizer efficiency and the effect of chesson structure is arranged.The used husk of present embodiment is rolled over the rice field by Wang Jia shop, Wuhan City, Hubei Province Jiu Feng township and is bought.
Below be specific embodiment of the present invention, technical scheme of the present invention is done further the description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
The light substrate seedling matrix formulations of embodiment 1 oil tea screening study test
For understanding the upgrowth situation of oil-tea sprout stock grafting seedling in the material mesh bag container of filling different light matrix; Thereby define through this advance copy inventor and to be beneficial to the cultivation matrix that oil tea grows, for the industrialized development of growing seedlings of the light base material mesh bag container of China oil tea provides practicable matrix formulations.
1, test material
The long woods series of products of the oil tea improved seeds branch of selecting the inferior woods in Chinese forest-science academy to be selected is scion; After carrying out the sprout stock grafting; Plant conduct confession examination material in the material mesh bag container of filling different light matrix compositions, the light base material mesh bag container seedling of the oil tea of mentioning below the inventor is all this series of products grafting.
2, test method
With peat soil, perlite is major ingredient, adds husk or loess simultaneously, is mixed into different light ground mass by different volume ratios and is filled in nutrition section that the mesh bag inscribe is slit into diameter 4 cm, height 10 cm as cultivating container as the packing material of material mesh bag container; 7 prescriptions are set, use respectively 1., 2.; 3., 4., 5.; 6., 7. expression.
1. number: peat 50% ﹢ perlite 40% ﹢ 10% husk
2. number: peat 50% ﹢ perlite 40% ﹢ 10% loess
3. number: peat 50% ﹢ perlite 30% ﹢ 10% husk ﹢ 10% loess
4. number: peat 50% ﹢ perlite 30% ﹢ 15% husk ﹢ 5% loess
5. number: peat 50% ﹢ perlite 30% ﹢ 20% husk
6. number: peat 50% ﹢ perlite 20% ﹢ 25% husk ﹢ 5% loess
7. number: peat 50% ﹢ perlite 20% ﹢ 20% husk ﹢ 10% loess
Below all be mixed by volume.
Different substrates is handled 3 times and is repeated, and whenever repeats to plant 30 strain oil tea graftings.The oil tea grafting is selected for use with the 1 batch of seed with 1 batch of fringe bar, and the plastic film strip colligation is used in grafting, on May 28th, 2010 by same individual grafting, the same time period plants, the consistent Routine Management way that adopts the light substrate seedling of oil tea of all control measures of later stage.Observe its survival rate, take out growth indexes such as tip rate, plant height and leading thread and investigate and measure and analyze mid-April next year, understand the influence of different light matrix container utilizations the light substrate seedling growth indexes of oil tea.This test variance analysis part is accomplished by SPSS variance analysis software, and 95% confidence interval is chosen in variance analysis.
3, result of the test and analysis
(1) different substrates is selected the influence to the light base material mesh bag container shoot survival percent of oil tea
Survival rate to the oil tea grafting in the material mesh bag container that is grown in different light matrix compositions is investigated investigation result such as table 1.The result shows: the survival rate of the oil tea grafting that grows in the different substrates is obviously different; 5. survival rate is descending is arranged as>1.>6.>7.>2.>4.>3.; Wherein 1., 5., 6. the survival rate of number matrix is all more than 90%; 5. the survival rate of number matrix has been up to 96.78%, and 1. number matrix survival rate has also reached 94.23%.
Table 1 different substrates is selected the influence to the light base material mesh bag container shoot survival percent of oil tea
Different substrates Survival rate (%)
94.23
82.36
80.34
81.64
96.78
90.77
88.89
(2) different substrates selects the light matrix container seedling of oil tea is taken out the influence of tip rate
The tip rate of taking out to the oil tea grafting in the material mesh bag container that is grown in different light matrix compositions is investigated, and investigation result is seen table 2.The result shows; The tip rate of taking out of the oil tea grafting that grows in the different light matrix is obviously different; Take out that tip rate is descending to be arranged as: 5.>1.>6.>4.>3.>2.>7., wherein 5., 1. the tip rate of taking out of number matrix has greater advantage with other matrix phase ratio; All surpassed 90%, 5. the tip rate of taking out of number matrix has been up to 91.22%.
Table 2 different substrates selects the light matrix container seedling of oil tea is taken out the influence of tip rate
Different substrates Take out rate slightly
90.75
72.66
75.61
78.52
91.22
80.59
70.44
(3) different substrates is selected the influence to the light matrix container seedling of oil tea plant height
Plant height to the oil tea grafting in the material mesh bag container that is grown in different light matrix compositions is investigated, and investigation result is seen table 3.
Table 3 different substrates is selected the influence to the light matrix container seedling of oil tea plant height
Different substrates Plant height (cm)
14.26
12.92
12.55
12.64
14.66
12.32
12.75
Growth has or not the significance influence to the light matrix grafting container of oil tea seedling plant height for further understanding different substrates is selected, and employing one-dimensional variance analysis method has been carried out variance analysis to the plant height of the light base material mesh bag container seedling of oil tea in the different substrates.The result sees table 4.
The light base material mesh bag container seedling of oil tea plant height variance analysis table in table 4 different substrates
Soruces of variation Average with Degree of freedom All square FValue PValue
Between-group variance 312.165 6 52.028 10.242 0
Intra-class variance 2057.366 405 5.080 - -
Total variation 2369.531 411 - - -
We have carried out multiple ratio, result such as table 5 with the Duccan method to the light matrix grafting container of the oil tea seedling plant height of cultivating in the light matrix of difference simultaneously.Variance analysis and multiple ratio result to the plant height of the light base material mesh bag container seedling of oil tea in the different substrates show: the height growth that light matrix container is grown seedlings on the whole to camellia seedling is favourable, and the oil tea seedling of growing in these 7 kinds of matrix all reaches or reach basically the growing height of I level seedling in the camellia seedling national standard.But also can find out in the different substrates between the growth of the light base material mesh bag container height of seedling of oil tea degree to exist significant difference, wherein 1. the light matrix phase of number matrix and 5. number matrix and other prescription than highly significant advantage being arranged aspect the growth the light base material mesh bag container grafting of promotion.
The light matrix grafting container of the oil tea seedling plant height The result of multiple comparisons of cultivating in the different light matrix of table 5
Different substrates Plant height (cm) α=0.05
14.26 a
12.92 b
12.55 b
12.64 b
14.66 a
12.32 b
12.75 b
(4) different substrates is selected the influence to the growth of the light base material mesh bag container seedling of oil tea leading thread
Leading thread to being grown in the light base material mesh bag container seedling of oil tea in the different substrates is investigated, and investigation result is seen table 6.From table 6, can find out: the growth of the leading thread of the living container seedling of light base material mesh bag 1 a of oil tea has all reached camellia seedling national standard I level seedling level (> 2.5 mm) more than, explain that carrying out the oil tea container nursery with light base material mesh bag compares with conventional camellia seedling on leading thread is grown very big advantage is arranged.
The light base material mesh bag container seedling of oil tea leading thread growth investigation in table 6 different substrates
Different substrates Leading thread (mm)
3.48
3.11
3.23
2.89
3.28
2.88
3.07
Growth has or not the significance influence to the light matrix grafting container of oil tea seedling leading thread in order further to understand the different substrates selection, and the inventor adopts the one-dimensional variance analysis method that the leading thread that is grown in the light base material mesh bag container seedling of oil tea in the different substrates has been carried out variance analysis.The result sees table 7.
The light base material mesh bag container seedling of oil tea leading thread variance analysis table in table 7 different substrates
Soruces of variation Average with Degree of freedom All square FValue PValue
Between-group variance 16.559 6 2.760 32.062 0
Intra-class variance 34.860 405 0.086 - -
Total variation 51.419 411 - - -
Simultaneously we have carried out multiple ratio, result such as table 8 with the Duccan method to the leading thread of the light matrix grafting container of the oil tea seedling of cultivating in the light matrix of difference.
The light base material mesh bag container seedling of oil tea leading thread growth The result of multiple comparisons in table 8 different substrates
Different substrates Leading thread (mm) α=0.05
3.48 a
3.11 b
3.23 b
2.89 b
3.28 a
2.88 b
3.07 b
The variance analysis and the multiple ratio result that take out the growth of tip rugosity to the light base material mesh bag container seedling of oil tea in the different substrates show: exist significant difference between the growth of the light base material mesh bag container seedling of oil tea leading thread in the different substrates; Wherein 1. number matrix has significant advantage with other matrix phase on growing in the promotion leading thread, and 5., 3. number matrix is taken second place; 2.; 7. number matrix is taken second place again, and 4., 6. number matrix is remarkable not as other matrix advantage in the growth of promotion nursery stock leading thread.Explain that 1. number matrix is promoting that in the growth of the light base material mesh bag container seedling of oil tea leading thread be optimum; 3., 5. number matrix also is suitable for the leading thread growth that promotes the light base material mesh bag container seedling of oil tea.
Comprehensive above result of the test can draw as drawing a conclusion:
(1) selection of cultivation matrix is influence one of result's the key factor of growing seedlings in the light base material mesh bag container seedling raising process of oil tea, the survival rate of the light base material mesh bag container seedling of the oil tea in the different substrates, takes out tip rate and the growth performance all has difference in various degree.
1. number (2) survival rate of the light base material mesh bag container seedling of the oil tea between different cultivation matrixes is significantly different, and 5. the cultivation matrix that survival rate is high have,, and their survival rate is all more than 94%; The tip rate of taking out of the light base material mesh bag container seedling of oil tea that different cultivation matrixes are asked is also variant, takes out wherein that tip rate reaches 90% cultivation matrix and has 5., 1. number matrix.
(3) exist significant difference between the plant height of the different cultivation matrixes light base material mesh bag container seedling of oil tea of asking and the leading thread, 1., 5. number matrix has significant advantage to the height growth that promotes light base material mesh bag container grafting; 1. number matrix is to promoting the light base material mesh bag container seedling of oil tea leading thread growth optimum, 3., 5. number matrix is taken second place.
(4) comprehensively above can find out the light base material mesh bag container shoot survival percent of oil tea in the different substrates, the analysis result of taking out tip rate and growth indexes; 1. number matrix and 5. number matrix all performance is good on each item growth indexes that the light base material mesh bag container of oil tea is grown seedlings, can be used as demonstration matrix formulations wide popularization and application in the light substrate seedling of oil tea is produced of the light base material mesh bag container cultivation of oil tea.

Claims (3)

1. a matrix formulations that is used for the light substrate seedling of oil tea is characterized in that: be made up of following components in percentage by volume: peat 45%-55%, perlite 30%-40% and husk 10%-20%.
2. the matrix formulations that is used for the light substrate seedling of oil tea according to claim 1 is characterized in that: be made up of following components in percentage by volume: peat 50%, perlite 40% and husk 10%.
3. the matrix formulations that is used for the light substrate seedling of oil tea according to claim 1 is characterized in that: be made up of following components in percentage by volume: peat 50%, perlite 30% and husk 20%.
CN2011104463174A 2011-12-28 2011-12-28 Medium formula of light medium seedling for Camellia oleifera Abel. Pending CN102550378A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082109A (en) * 2014-06-11 2014-10-08 浙江兰特园艺有限公司 Container cultivation method for nursery stock
CN105284478A (en) * 2015-11-19 2016-02-03 贺友德 One-step cultivation method for Camellia oleifera bud-stock light matrix grafting container seedlings
CN106034820A (en) * 2016-07-28 2016-10-26 桐城市牯牛背农业开发有限公司 Oil-tea-tree-seedling cultivating medium and preparing method thereof
CN106342581A (en) * 2016-08-23 2017-01-25 福建画龙点睛园林集团股份有限公司 Light planting medium for roof greening
CN109429987A (en) * 2018-11-29 2019-03-08 安徽泓森高科林业股份有限公司 A kind of locust tree root cutting growth matrix

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101366356A (en) * 2008-09-25 2009-02-18 浙江省亚热带作物研究所 Complex interstitial substance for cyclobalanopsis glauca container raise seedling
CN101611681A (en) * 2009-07-17 2009-12-30 广西壮族自治区林业科学研究院 The propagation method of using single-bud short tamarix to cut containerized seedlings
CN101810112A (en) * 2010-04-26 2010-08-25 湖南省烟草公司永州市公司 New medium for floating seedling raising of flue-cured tobacco

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101366356A (en) * 2008-09-25 2009-02-18 浙江省亚热带作物研究所 Complex interstitial substance for cyclobalanopsis glauca container raise seedling
CN101611681A (en) * 2009-07-17 2009-12-30 广西壮族自治区林业科学研究院 The propagation method of using single-bud short tamarix to cut containerized seedlings
CN101810112A (en) * 2010-04-26 2010-08-25 湖南省烟草公司永州市公司 New medium for floating seedling raising of flue-cured tobacco

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
彭邵锋等: "不同育苗基质对油茶良种容器苗生长的影响", 《中南林业科技大学学报》 *
章尧想等: "油茶轻基质育苗技术研究", 《林业实用技术》 *
陈华超等: "油茶轻基质育苗基质配方筛选研究", 《湖北林业科技》 *
陈慧玲等: "栽培基质对油茶芽苗砧嫁接容器苗生长的影响", 《林业科技开发》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082109A (en) * 2014-06-11 2014-10-08 浙江兰特园艺有限公司 Container cultivation method for nursery stock
CN104082109B (en) * 2014-06-11 2016-06-15 浙江兰特园艺有限公司 A kind of cultivation container method of nursery stock
CN105284478A (en) * 2015-11-19 2016-02-03 贺友德 One-step cultivation method for Camellia oleifera bud-stock light matrix grafting container seedlings
CN105284478B (en) * 2015-11-19 2018-04-17 贺友德 A kind of one-step method breeding method of oil-tea sprout anvil Light media grafting container seedling
CN106034820A (en) * 2016-07-28 2016-10-26 桐城市牯牛背农业开发有限公司 Oil-tea-tree-seedling cultivating medium and preparing method thereof
CN106342581A (en) * 2016-08-23 2017-01-25 福建画龙点睛园林集团股份有限公司 Light planting medium for roof greening
CN109429987A (en) * 2018-11-29 2019-03-08 安徽泓森高科林业股份有限公司 A kind of locust tree root cutting growth matrix

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Application publication date: 20120711