CN103340045A - Method for water separation and sterilization of tobacco seeds - Google Patents
Method for water separation and sterilization of tobacco seeds Download PDFInfo
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- CN103340045A CN103340045A CN2013102946136A CN201310294613A CN103340045A CN 103340045 A CN103340045 A CN 103340045A CN 2013102946136 A CN2013102946136 A CN 2013102946136A CN 201310294613 A CN201310294613 A CN 201310294613A CN 103340045 A CN103340045 A CN 103340045A
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Abstract
The invention discloses a method for water separation and sterilization of tobacco seeds. The method comprises the following steps of: 1, soaking, namely preparing cloth bags in advance, packaging the seeds into the cloth bags, tying the openings of the bags, and soaking the bags in clean water at normal temperature; 2, removing impurities, namely enabling the opening of each cloth bag to pass through a tubular sleeve, folding the opening downwards, and putting the bags into a flow tank, so that the shrunken seeds and peels float and overflow from the upper surface to flow away along with water; 3, sterilizing, namely tying the openings of the cloth bags after the impurities are removed, and disinfecting the seeds in a disinfection pool with a disinfectant; 4, cleaning, namely fishing out all the seed bags, agitating the bags in a cleaning pool for cleaning for twice, and rinsing the disinfectant by running water; 5, drying, namely fishing out the cleaned seed bags, putting the bags on a filter frame, and putting the bags into an air dry oven to dry after no water drop is generated; and 6, storing. The method disclosed by the invention can be used for performing water separation and sterilization on a plurality of parts of few tobacco seeds, the quality of the tobacco seeds is improved, and the situation that the seeds do not have bacteria is ensured, thereby laying foundation for planting of excellent tobaccos. The method is suitable for the tobacco planting industry.
Description
Technical field
The present invention relates to seed processing technology, relate to seed selection, furthermore, relate to the sterilization of seed, relate in particular to wet concentration and the sterilization of tobacco seed.
Background technology
In tobacco breeding work, must a large amount of hybrid combinations of preparation and a large amount of fine individual plants of selection.The seed umber of receiving more, every part of seed amount is few.This need solve the wet concentration technology, and can carry out disinfection to tobacco seed simultaneously.In the work of introducing a fine variety, need quarantine, quarantine with the method for planting experimentally, disease is remained in isolated area; Connection with wrapping of piece is carried out disinfection, seed is carried out the wet concentration sterilization, through the aseptic culture check, just can the fine purpose that reaches quarantine.In variety source is collected, by wet concentration and sterilization, guarantee seed quality, prevent seed-borne fungi, disease is propagated in the strange land.In germ plasm resource is stored, through wet concentration and sterilization, can guarantee seed quality, prevent that present disease from taking place in future again.
At present, in Chinese patent database, the application part that relates to tobacco seed wet concentration, sterilization is few, and No. 201220083994.4 " tobacco seed wet concentration sterilizer " and No. 201220084033.5 " a kind of Sheng kind jar for tobacco seed wet concentration and sterilization " two only arranged.The exploitation of correlation technique is necessary.
Summary of the invention
The object of the present invention is to provide a kind of tobacco seed wet concentration sterilization method, it can be used for wet concentration and the sterilization of tobacco seed, thereby improves seed quality, and guarantees that seed does not carry disease germs.
The tobacco seed wet concentration disinfection technology that the inventor provides comprises:
The first step is soaked
Prepare cloth bag in advance, will need some parts of seeds of wet concentration sterilization be respectively charged into cloth bag, the every packed seed of sack heap(ed) capacity below 1/2 of going into; Tie sack, put into the clear water soak at room temperature, all cloth bags are dipped in below the water surface;
Second step, impurity elimination
After the immersion, the cloth sack is passed in the previously prepared tubular sleeve, sack turns down downwards, one by one sleeve is put into another pond, and sack is a little less than the water surface, and not full seed and pericarp float, and floating thing is overflowed with current from above flow away;
The 3rd step, sterilization
The seed packet of impurity elimination is tied the cloth sack, put into the sterilization pool that thimerosal is housed, in the complete submergence thimerosal of seed packet, carry out disinfection;
In the 4th step, clean
From sterilization pool, pull all seed packets out, put into service sink together, under agitation clean 2 times, wash with flowing water again;
The 5th step, oven dry
The seed packet that cleans up pulled out be placed on the filter stand, wait not have and put into air dry oven after dripping and dry, make seed moisture content reach 7%～8% and get final product;
In the 6th step, store
With the seed of wet concentration sterilizing-drying, store by various objectives branch short-term, medium and long term.
In the above-mentioned first step, described soak time is in 4 hours.
In above-mentioned the 3rd step, described thimerosal is a kind of in 75% alcohol, 10% hydrogen peroxide, 2% available chlorine liquor natrii hypochloritis, 1 ‰ mercuric chloride, 1% copper sulphate, 0.1% silver nitrate.
The inventor points out that thimerosal also can adopt corresponding bactericide to carry out drug disinfection according to the specific pathogen bacterium, namely selects different thimerosals for use according to various objectives.
In above-mentioned the 4th step, described flowing water washing time is controlled to be 20 minutes, is as the criterion so that thimerosal is rinsed well.
In above-mentioned the 5th step, the control of the temperature of described oven dry is at 36 ℃～38 ℃, the highlyest is no more than 38 ℃.
Method of the present invention can be used for wet concentration and the sterilization to many parts of a small amount of tobacco seeds, improves the tobacco seed quality, guarantees that seed does not carry disease germs, for plantation high-quality tobacco lays the foundation.Be applicable to the tobacco planting industry.
Embodiment
Embodiment: fine individual plant seed wet concentration, sterilization
In tobacco breeding work, a large amount of fine individual plants of annual screening, hundreds of parts at least, thousands of parts at most, through taking off the seed after planting, have the part pericarp, the seed that has is not full, and pericarp may have mosaic disease (the not wounded in the battle leaf disease of tobacco seed) or other pathogen.
At first, with all seeds of gathering in the crops then, be respectively charged into cloth bag, the every packed seed of sack heap(ed) capacity below 1/2 of going into; Tie sack, hold with a container, the dress clear water soaked 2 hours, guaranteed that all sacks are in the underwater;
After the immersion, one by one cloth bag is taken out, put previously prepared tubular sleeve, put into a basin, be connected to water pipe on the basin, constantly discharge water, floating thing is overflowed flow away, after having cleared up, tie sack, after the seed of all immersions has been cleared up, put into sterilization pool;
Afterwards, preparation 10%H
2O
2Thimerosal, the seed packet of sterilization pool is put in submergence fully, and soak time is no more than 10 minutes;
Then, outwell thimerosal, stir flushing 2 times with clear water, with flowing water flushing 20 minutes;
After this, the seed packet that wet concentration is disinfected is placed on drip-dry on the filter stand, puts into air dry oven after nothing is dripped, and the control wind-warm syndrome was dried by the fire 5.5 hours down at 36 ℃～38 ℃, made seed moisture content reach 7%～8% and got final product
At last, the seed of fine individual plant only needs short-term to preserve, and for the next year use, deposits well with common tobacco seed is packed.
Claims (5)
1. tobacco seed wet concentration sterilization method, its feature comprises:
The first step is soaked
Prepare cloth bag in advance, will need some parts of seeds of wet concentration sterilization be respectively charged into cloth bag, the every packed seed of sack heap(ed) capacity below 1/2 of going into; Tie sack, put into the clear water soak at room temperature, all cloth bags are dipped in below the water surface;
Second step, impurity elimination
After the immersion, the cloth sack is passed in the previously prepared tubular sleeve, sack turns down downwards, one by one sleeve is put into another pond, and sack is a little less than the water surface, and not full seed and pericarp float, and floating thing is overflowed with current from above flow away;
The 3rd step, sterilization
The seed packet of impurity elimination is tied the cloth sack, put into the sterilization pool that thimerosal is housed, in the complete submergence thimerosal of seed packet, carry out disinfection;
In the 4th step, clean
From sterilization pool, pull all seed packets out, put into service sink together, under agitation clean 2 times, wash with flowing water again;
The 5th step, oven dry
The seed packet that cleans up pulled out be placed on the filter stand, wait not have and put into air dry oven after dripping and dry, make seed moisture content reach 7%～8% and get final product;
In the 6th step, store
With the seed of wet concentration sterilizing-drying, store by various objectives branch short-term, medium and long term.
2. the method for claim 1 is characterized in that in the first step, and described soak time is in 4 hours.
3. the method for claim 1 is characterized in that in the 3rd step, and described thimerosal is a kind of in 75% alcohol, 10% hydrogen peroxide, 2% available chlorine liquor natrii hypochloritis, 1 ‰ mercuric chloride, 1% copper sulphate, 0.1% silver nitrate.
4. the method for claim 1 is characterized in that described flowing water washing time is controlled to be 20 minutes, is as the criterion to rinse thimerosal well in the 4th step.
5. the method for claim 1 is characterized in that in the 5th step, and the temperature control of described oven dry is at 36 ℃～38 ℃, the highlyest was no more than 38 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102946136A CN103340045A (en) | 2013-07-15 | 2013-07-15 | Method for water separation and sterilization of tobacco seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102946136A CN103340045A (en) | 2013-07-15 | 2013-07-15 | Method for water separation and sterilization of tobacco seeds |
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| CN103340045A true CN103340045A (en) | 2013-10-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2013102946136A Pending CN103340045A (en) | 2013-07-15 | 2013-07-15 | Method for water separation and sterilization of tobacco seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340414A (en) * | 2015-11-20 | 2016-02-24 | 玉溪中烟种子有限责任公司 | Method for sorting tobacco seeds by using density of organic media |
| CN106942204A (en) * | 2017-03-20 | 2017-07-14 | 四川省烟草公司凉山州公司 | A kind of storage practice of tobacco seed |
| CN114051787A (en) * | 2021-11-15 | 2022-02-18 | 玉溪中烟种子有限责任公司 | Tobacco seed bleaching and sterilizing method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0269106A (en) * | 1988-09-05 | 1990-03-08 | Nisshoku Corp | Seeding equipment |
| CN101185388A (en) * | 2007-11-12 | 2008-05-28 | 贵州省烟草科学研究所 | Accelerating germination technology for tobacco seed |
| CN101611663A (en) * | 2009-07-24 | 2009-12-30 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | A kind of tobacco seed germination accelerating method |
-
2013
- 2013-07-15 CN CN2013102946136A patent/CN103340045A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0269106A (en) * | 1988-09-05 | 1990-03-08 | Nisshoku Corp | Seeding equipment |
| CN101185388A (en) * | 2007-11-12 | 2008-05-28 | 贵州省烟草科学研究所 | Accelerating germination technology for tobacco seed |
| CN101611663A (en) * | 2009-07-24 | 2009-12-30 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | A kind of tobacco seed germination accelerating method |
Non-Patent Citations (1)
| Title |
|---|
| 孙福山: "烟草播种前种子处理技术", 《农业科技通讯》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340414A (en) * | 2015-11-20 | 2016-02-24 | 玉溪中烟种子有限责任公司 | Method for sorting tobacco seeds by using density of organic media |
| CN105340414B (en) * | 2015-11-20 | 2018-06-29 | 玉溪中烟种子有限责任公司 | Utilize the method for organic media density separation tobacco seed |
| CN106942204A (en) * | 2017-03-20 | 2017-07-14 | 四川省烟草公司凉山州公司 | A kind of storage practice of tobacco seed |
| CN106942204B (en) * | 2017-03-20 | 2021-01-22 | 四川省烟草公司凉山州公司 | Storage method of tobacco seeds |
| CN114051787A (en) * | 2021-11-15 | 2022-02-18 | 玉溪中烟种子有限责任公司 | Tobacco seed bleaching and sterilizing method |
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Application publication date: 20131009 |