CN101822271A - Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof - Google Patents
Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof Download PDFInfo
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- CN101822271A CN101822271A CN 201010169900 CN201010169900A CN101822271A CN 101822271 A CN101822271 A CN 101822271A CN 201010169900 CN201010169900 CN 201010169900 CN 201010169900 A CN201010169900 A CN 201010169900A CN 101822271 A CN101822271 A CN 101822271A
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Abstract
The invention discloses an acidizing fluid for green algae and a disease infected cell in porphyra haitanensis cultivation and a treating method thereof. A solvent for the acidizing fluid is clean seawater, and solute for the acidizing fluid is at least one of hydrochloric acid and citric acid, wherein the concentration expressed in percentage by weight of the solute in the acidizing fluid is 0.3 to 1.5 percent. The green algae and the disease infected cell can be quickly killed by a treating method of preparing the acidizing fluid, shipping, dipping for 15 to 20 seconds and rinsing, killing effect is better, the killing rate is over 90 percent, and the influence on the growth of the porphyra haitanensis is less. The treating method has the advantages of no restriction of natural condition, labor intensity reduction and the like.
Description
Technical field
The present invention relates to Porphyra haitanensis and culture the processing of the green algae and the cell of catching an illness, be specifically related to pickling agent and processing method that Porphyra haitanensis is cultured the green algae and the cell of catching an illness.
Background technology
The Porphyra haitanensis of culturing has two kinds of phenomenons often to take place, and the first breaks out large-scale green alga (as Enteromorpha etc.), competitive growth and the output that suppresses Porphyra haitanensis in Porphyra haitanensis cultivation marine site; It two is the cell of catching an illness to have occurred in the part of Porphyra haitanensis frond, cell is untimely if catch an illness handles, germ in the open sore part is with rapid spread, and then expand to other positions of frond, it is mashed to develop into spreading venereal diseases from physiological disturbance, feed through to breed that face mutually or even whole sea area rapidly, the growth of Porphyra haitanensis and output descend.
Handle the green alga and the alternative method of the cell of catching an illness of culturing in the Porphyra haitanensis at present the net method of solarization and refrigeration are arranged, traditional solarization net method is to adopt continuous time a couple of days airing lace curtaining, dehydrate and make green alga and the dry death of the cell of catching an illness, but the degree of airing is difficult to control, the possible green alga and the cell of catching an illness are all dead, also the possibility green alga and the cell death of catching an illness are less, shine the net method and limited by weather and morning and evening tides, also make the Porphyra haitanensis retarding of growing 3～5 days.Refrigeration is that the lace curtaining that Porphyra haitanensis is cultured is dried in the shade, the breed of plunging into the commercial sea again after sealing refrigerates several days, and refrigeration can be killed most of green alga and the cell of catching an illness, but the difficult control of seedling net humidity, it is rotten to cross the wet Porphyra haitanensis that causes easily, and overdrying makes the thallus of porphyra fragmentation, influences the laver further growth; Refrigeration need be taken off the laver lace curtaining from culture raft frame, fasten culture raft frame after the processing again, and this also makes processing procedure need a large amount of labours, also easily causes problems such as seedling partly comes off.
Summary of the invention
Technical problem to be solved by this invention provide a kind of can kill Porphyra haitanensis in culturing green alga and the pickling agent of the cell of catching an illness, it is short that this pickling agent is handled the time of the green alga and the cell of catching an illness, and killing effect is better, handles through 15～120 seconds, and killing rate can reach more than 90%.
The present invention also provides with this pickling agent and has handled the method for culturing the Porphyra haitanensis green algae and the cell of catching an illness, and this method has fast, and killing effect is better, and is less to the Porphyra haitanensis growth effect, not limited by natural conditions, reduced advantages such as labour intensity.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: Porphyra haitanensis is cultured the pickling agent of the green algae and the cell of catching an illness, the solvent of this pickling agent is a clean sea water, the solute of this pickling agent be hydrochloric acid and citric acid at least wherein to one, the concentration expressed in percentage by weight of solute is 0.3～1.5% in this pickling agent.
The solute of this pickling agent is made up of by 1: 1 weight ratio hydrochloric acid and citric acid, and the acid of two kinds of hydrochloric acid and citric acids mixes, kill green alga and the cell of catching an illness better with respect to single with the citric acid effect, to the normal somatic cell of Porphyra haitanensis with respect to single with the hydrochloric acid infringement still less.
The concentration expressed in percentage by weight of solute is 0.6～1.0% in this pickling agent; This concentration can better be killed green alga and the cell of catching an illness, and makes the normal somatic cell infringement of Porphyra haitanensis few again.
Also contain 1～3 sodium polyphosphate that restrains in every liter of this pickling agent; Sodium polyphosphate as bleeding agent, can allow pickling agent comparatively fast enter green alga and the cell of catching an illness at this.
Also contain 0.2～0.5 tween that restrains in every liter of this pickling agent; Tween has Action of Surfactant at this, can prevent from obviously to reduce at the silt of laver surface deposition the concentration of treatment fluid, influences the effect of treatment effect.
Pickling agent is handled the method that Porphyra haitanensis is cultured the green algae and the cell of catching an illness, and comprises the steps:
1) pickling agent preparation: or/and citric acid is dissolved in the clean sea water, the concentration expressed in percentage by weight that is mixed with solute is 0.3～1.5% pickling agent with hydrochloric acid; Or again in every liter that is made into described pickling agent, add the sodium polyphosphate of 1～3 gram and the tween of 0.2～0.5 gram;
2) shipment: in the storehouse with the described pickling agent side of installing to ship, square again ship places the below of the lace curtaining of culturing Porphyra haitanensis;
3) soak: the lace curtaining and the Porphyra haitanensis of marine breed Porphyra haitanensis are picked up draining to closely not dripping, and lace curtaining and the Porphyra haitanensis that will culture Porphyra haitanensis then immerse in the described pickling agent, the vibrations lace curtaining, and soak time is 15～120 seconds;
4) waft and wash: soak finish after, in time will culture the lace curtaining of Porphyra haitanensis and Porphyra haitanensis again and waft and wash the pickling agent in the flush away Porphyra haitanensis; Waft wash can put into marine waft naturally to wash or manually waft gather after washing; Can put into after treatment at the Porphyra haitanensis in vegetative period and marinely to allow it waft naturally to wash and culture, if behind the treated removing of the Porphyra haitanensis of the picking time green alga and the cell of catching an illness, can be through the acid solution of the flush away normal somatic cell that manually wafts, the green alga and the cell of being killed of catching an illness, gathering obtains quality Porphyra haitanensis preferably.
The concentration expressed in percentage by weight of described pickling agent solute is 0.6～1%; Soak time is 30～90 seconds, this concentration can better be killed green alga and the cell of catching an illness, make the normal somatic cell infringement of Porphyra haitanensis few again, specific acid treatment fluid concentration and soak time are according to the size of Porphyra haitanensis, the quantity positive correlation of the green alga and the cell of catching an illness is grasped, at any time the color and luster of noting green alga in immersion process changes, green alga becomes blackish green or yellow green from emerald green and soaks and can finish, if green alga is that biomass is when big, preferably handled again and once come further to kill green alga and the cell of catching an illness every several days, do not make long soaking time, cause the normal somatic cell damage of Porphyra haitanensis bigger.
Compared with prior art, the invention has the advantages that the pickling agent of the Porphyra haitanensis breed green algae and the cell of catching an illness, the solvent of this pickling agent is a clean sea water, the solute of this pickling agent be hydrochloric acid and citric acid at least wherein to one, the concentration expressed in percentage by weight of solute is 0.3～1.5% in this pickling agent; This is different to sour suffertibility with catch an illness cell and assorted algae according to the normal somatic cell of Porphyra haitanensis, it is better to choose killing effect through a large amount of tests, need the less pickling agent of normal somatic cell infringement, the green alga and the cell of catching an illness have bigger permeability than normal somatic cell to this pickling agent, this pickling agent can enter green alga fast and catches an illness in the cell at the utmost point in the short time, cause green alga and the protoplasm of cell of catching an illness in protein denaturation and solidify, make green alga and the cell inactivation of catching an illness is killed; And the pickling agent amount of penetrating of normal somatic cell is few, and the time is short, and therefore infringement is few.Pickling agent is handled the method for culturing the Porphyra haitanensis green algae and the cell of catching an illness and is by pickling agent preparation, shipment, soaked 15～120 seconds and waftd and wash, both can fast the green alga and the cell of catching an illness be killed, killing effect is better, less to the Porphyra haitanensis growth effect again, this method is not limited by natural conditions in addition, has reduced advantages such as labour intensity.Therefore the present invention be a kind of can kill Porphyra haitanensis in culturing green alga and the pickling agent and the processing method of the cell of catching an illness, it is short that this pickling agent has the time of handling the green alga and the cell of catching an illness, and killing effect is better, killing rate can reach more than 90%, less to the Porphyra haitanensis growth effect, not limited by natural conditions, reduced advantages such as labour intensity.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
Porphyra haitanensis is cultured the pickling agent and the processing method of the green algae and the cell of catching an illness, with 5 kilograms of hydrochloric acid and 5 kilograms of citric acids is that solute is dissolved in 1200 kilograms of clean sea waters, the concentration expressed in percentage by weight of preparation solute is about 0.826 pickling agent, in pickling agent, be added with the sodium polyphosphate of 2500 grams and the tween of 240 grams again, so just can handle Porphyra haitanensis and culture the green algae and the cell of catching an illness with this pickling agent.In the ship storehouse with the above-mentioned pickling agent side of packing into ship, in the ship storehouse, can fill up rainproof film in advance, prevent that pickling agent from leaking, square ship's ship is reached Porphyra haitanensis culture ground, place the below of the lace curtaining of culturing Porphyra haitanensis, lace curtaining is picked up draining together with Porphyra haitanensis, when seldom dripping, lace curtaining is immersed in the pickling agent together with Porphyra haitanensis, and soaked 30～90 seconds on vibrations lace curtaining limit, limit, after immersion is finished, in time will culture the lace curtaining of Porphyra haitanensis and Porphyra haitanensis again puts into marine wafing naturally and washes, if the Porphyra haitanensis that can gather just can be gathered after then the direct labor is waftd and washed and be dried, the killing rate of the green alga and the cell of catching an illness can reach more than 90%.Soak time and Porphyra haitanensis size, how many green algas becomes positive correlation with the cell of catching an illness, and Porphyra haitanensis hour soak time should be grasped shorter relatively, prevents that pickling agent from injuring normal somatic cell, otherwise just can soak with the relative long period; Green alga and catch an illness cell for a long time, long period soaks relatively, need in the time of too much can soak again every several days, expect disposablely to kill green alga and prolong and surpass to soak limiting time, can bring than macrolesion the laver normal somatic cell like this, therefore in 15～120 scopes of the present invention, the color and luster of observing green alga while soaking changes in the concrete immersion process, and green alga becomes blackish green or the yellow green immersion can finish from emerald green.
Embodiment 2
Substantially the same manner as Example 1, different just 4 kilograms of citric acids be that solute is dissolved in 1200 kilograms of clean sea waters, the sodium polyphosphate addition is 3000 grams, the tween addition is 600 grams, and lace curtaining is 60～120 seconds together with the soak time of Porphyra haitanensis, and soak time becomes negative correlation with the concentration of pickling agent, when concentration is big, soak time is corresponding shorter, concentration hour, and soak time is corresponding longer.
Embodiment 3
Substantially the same manner as Example 1, different just 18 kilograms of hydrochloric acid be that solute is dissolved in 1200 kilograms of clean sea waters, do not add sodium polyphosphate and tween, lace curtaining is 15～30 seconds together with the soak time of Porphyra haitanensis, soak time becomes negative correlation with the concentration of pickling agent, and when concentration was big, soak time was corresponding shorter, concentration hour, soak time is corresponding longer.
Embodiment 4
Substantially the same manner as Example 1, different just 4 kilograms of hydrochloric acid and 4 kilograms of citric acids be that solute is dissolved in 1200 kilograms of clean sea waters, the sodium polyphosphate addition is 1250 grams, the tween addition is 360 grams.
Claims (7)
1. Porphyra haitanensis is cultured the pickling agent of the green algae and the cell of catching an illness, the solvent that it is characterized in that this pickling agent is a clean sea water, the solute of this pickling agent be hydrochloric acid and citric acid at least wherein to one, the concentration expressed in percentage by weight of solute is 0.3～1.5% in this pickling agent.
2. Porphyra haitanensis as claimed in claim 1 is cultured the pickling agent of the green algae and the cell of catching an illness, and it is characterized in that the solute of this pickling agent is made up of by 1: 1 weight ratio hydrochloric acid and citric acid.
3. Porphyra haitanensis as claimed in claim 1 is cultured the pickling agent of the green algae and the cell of catching an illness, and the concentration expressed in percentage by weight that it is characterized in that solute in this pickling agent is 0.6～1.0%.
4. Porphyra haitanensis as claimed in claim 1 is cultured the pickling agent of the green algae and the cell of catching an illness, and it is characterized in that also containing in every liter of this pickling agent the sodium polyphosphate of 1～3 gram and the tween of 0.2～0.5 gram.
5. utilize claim 1 or 4 described pickling agents to handle the method that Porphyra haitanensis is cultured the green algae and the cell of catching an illness, it is characterized in that comprising the steps:
1) pickling agent preparation: with hydrochloric acid or/and citric acid be dissolved in the clean sea water, the concentration expressed in percentage by weight of preparation solute is 0.3～1.5% pickling agent, or again in every liter that is made into described pickling agent, add the sodium polyphosphate of 1～3 gram and the tween of 0.2～0.5 gram;
2) shipment: in the storehouse with the described pickling agent side of installing to ship, square again ship places the below of the lace curtaining of culturing Porphyra haitanensis;
3) soak: the lace curtaining and the Porphyra haitanensis of marine breed Porphyra haitanensis are picked up draining to closely not dripping, and lace curtaining and the Porphyra haitanensis that will culture Porphyra haitanensis then immerse in the described pickling agent, the vibrations lace curtaining, and soak time is 15～120 seconds;
4) waft and wash: in time will culture the lace curtaining of Porphyra haitanensis and Porphyra haitanensis again and waft and wash the pickling agent in the flush away Porphyra haitanensis.
6. pickling agent as claimed in claim 5 is handled the method that Porphyra haitanensis is cultured the green algae and the cell of catching an illness, and it is characterized in that in step 1 concentration expressed in percentage by weight of described pickling agent solute is 0.6～1%, and in step 3, soak time is 30～90 seconds.
7. pickling agent as claimed in claim 5 is handled the method that Porphyra haitanensis is cultured the green algae and the cell of catching an illness, it is characterized in that described waft to wash to putting into marine wafing naturally wash.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101699000A CN101822271B (en) | 2010-05-11 | 2010-05-11 | Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof |
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| CN2010101699000A CN101822271B (en) | 2010-05-11 | 2010-05-11 | Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof |
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| CN101822271A true CN101822271A (en) | 2010-09-08 |
| CN101822271B CN101822271B (en) | 2012-11-21 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550388A (en) * | 2012-01-06 | 2012-07-11 | 上海海洋大学 | Method for removing green algae in the middle and late culture stages of Porphyra yezoensis |
| CN103348813A (en) * | 2013-07-03 | 2013-10-16 | 宁波大学 | Porphyra haitanensis harvesting and acid processing integrated device |
| CN103609427A (en) * | 2013-12-02 | 2014-03-05 | 连云港市海洋与水产科学研究所 | Acid treatment type full-floating laver culture method |
| CN107114401A (en) * | 2017-03-29 | 2017-09-01 | 岳阳云秋珍珠科技有限公司 | A kind of Cyanobacterial scavenger and preparation method thereof |
| CN107262439A (en) * | 2017-06-28 | 2017-10-20 | 中国科学院海洋研究所 | A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining |
| JP2018093824A (en) * | 2016-12-15 | 2018-06-21 | 光洋通商株式会社 | Box ship |
| JP2018099073A (en) * | 2016-12-20 | 2018-06-28 | 光洋通商株式会社 | Laver net treatment ship |
| JP2018099079A (en) * | 2016-12-20 | 2018-06-28 | 光洋通商株式会社 | Boxy ship |
| CN108751272A (en) * | 2018-05-18 | 2018-11-06 | 中国科学院海洋研究所 | A kind of method of acid processing Enteromorpha under low-salt conditions |
| JP2019062884A (en) * | 2017-09-28 | 2019-04-25 | キュアース インコーポレイテッド | Laver processor using sonicator, and laver processing method using sonicator |
| CN113925058A (en) * | 2021-11-01 | 2022-01-14 | 上海海洋大学 | Mixed acid treatment solution for removing sessile green algae on porphyra yezoensis cultivation raft and method for removing green algae by using mixed acid treatment solution |
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Patent Citations (2)
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550388A (en) * | 2012-01-06 | 2012-07-11 | 上海海洋大学 | Method for removing green algae in the middle and late culture stages of Porphyra yezoensis |
| CN102550388B (en) * | 2012-01-06 | 2013-10-09 | 上海海洋大学 | Method for removing green algae in the middle and late culture stages of Porphyra yezoensis |
| CN103348813A (en) * | 2013-07-03 | 2013-10-16 | 宁波大学 | Porphyra haitanensis harvesting and acid processing integrated device |
| CN103609427A (en) * | 2013-12-02 | 2014-03-05 | 连云港市海洋与水产科学研究所 | Acid treatment type full-floating laver culture method |
| CN103609427B (en) * | 2013-12-02 | 2016-06-22 | 连云港市海洋与水产科学研究所 | A kind of acid treatment formula Thallus Porphyrae floating method |
| JP7160399B2 (en) | 2016-12-15 | 2022-10-25 | 光洋通商株式会社 | box ship |
| JP2021126127A (en) * | 2016-12-15 | 2021-09-02 | 光洋通商株式会社 | Box ship |
| JP2018093824A (en) * | 2016-12-15 | 2018-06-21 | 光洋通商株式会社 | Box ship |
| JP2018099073A (en) * | 2016-12-20 | 2018-06-28 | 光洋通商株式会社 | Laver net treatment ship |
| JP2018099079A (en) * | 2016-12-20 | 2018-06-28 | 光洋通商株式会社 | Boxy ship |
| CN107114401A (en) * | 2017-03-29 | 2017-09-01 | 岳阳云秋珍珠科技有限公司 | A kind of Cyanobacterial scavenger and preparation method thereof |
| CN107262439A (en) * | 2017-06-28 | 2017-10-20 | 中国科学院海洋研究所 | A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining |
| JP2019062884A (en) * | 2017-09-28 | 2019-04-25 | キュアース インコーポレイテッド | Laver processor using sonicator, and laver processing method using sonicator |
| CN109717069A (en) * | 2017-09-28 | 2019-05-07 | 朝鲜大学校产学协力团 | Seaweed processing unit using supersonic generator and the seaweed processing method using supersonic generator |
| CN108751272A (en) * | 2018-05-18 | 2018-11-06 | 中国科学院海洋研究所 | A kind of method of acid processing Enteromorpha under low-salt conditions |
| CN113925058A (en) * | 2021-11-01 | 2022-01-14 | 上海海洋大学 | Mixed acid treatment solution for removing sessile green algae on porphyra yezoensis cultivation raft and method for removing green algae by using mixed acid treatment solution |
| CN113925058B (en) * | 2021-11-01 | 2023-09-08 | 上海海洋大学 | Mixed acid treatment solution for removing green algae fixed on Porphyra yezoensis cultivation raft frame and method for removing green algae |
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