CN106614186B - Small size pond cultural method in a kind of thick stage room of Penaeus Vannmei mark - Google Patents

Small size pond cultural method in a kind of thick stage room of Penaeus Vannmei mark Download PDF

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CN106614186B
CN106614186B CN201611161999.3A CN201611161999A CN106614186B CN 106614186 B CN106614186 B CN 106614186B CN 201611161999 A CN201611161999 A CN 201611161999A CN 106614186 B CN106614186 B CN 106614186B
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water
mark
small size
shrimp seedling
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CN106614186A (en
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刘永坚
田丽霞
方蔚平
牛津
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National Sun Yat Sen University
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

Small size pond cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps: S1. container and water body disinfection, the small size pond that the water carrying capacity of container used is 0.1~0.5 ton;S2. the disease-free shrimp seedling of health is chosen;S3. mark extensive cultivation is grown: the culture-cycle is 20~30 days, and preceding 8 days breeding processes do not change water, and EM bacterium of every other day splashing into container carries out changing water after 8 days daily, changes after water EM bacterium of splashing;First 8 days progress part shading treatments cultivate under natural light after 8 days;In breeding process, point feed for 12 times in required time daily, feeds interval every time 90~120 minutes;It is grown 3~5 days before terminating in mark extensive cultivation, vitamin c solution of splashing daily carries out intensive treatment to shrimp seedling.By the shrimp seedling high survival rate of the thick method raising of this mark, the speed of growth is fast.

Description

Small size pond cultural method in a kind of thick stage room of Penaeus Vannmei mark
Technical field
The present invention relates to aquaculture technical fields, and in particular, to small in a kind of thick stage room of Penaeus Vannmei mark Aquifer cultivation method.
Background technique
Last century late nineteen eighties, Penaeus Vannmei are introduced into China, fast due to growing, and premunition is strong and deep by feeding shrimp person It welcomes, is currently the important seawater in China and brackish culture shrimps, cultivation range is universal national and is mainly distributed on southern edge Haiti area becomes the main warp of wide farmers increasing both production and income since its speed of growth is fast, feed efficiency is high and economic benefit is big Ji source, has a vast market foreground.All shrimps, which will undergo the abnormal stage just in early growth period, can be carried out cultivation, for It at the process of young shrimp will be directly not with the mode that pond culture is directly docked from fertilized eggs metamorphosis for current cultivation situation It is feasible, because shrimp seedling is also in the very small and weak stage in young shrimp, young shrimp is directly placed into nature pond environment at this time and is supported Great shrimp seedling loss will be will cause by growing, and cultivation survival rate reduces, and aquaculture cost increases etc., therefore the mark for connecting two stages is thick Phase is just extremely important.
Mark at present the thick stage there are the problem of include: first is that disease is serious, entirety cultivation water environment is continuous severe at present Cause to mark thick stage shrimp seedling and be easy to be influenced by disease, the especially vibrios in seawater is maximum to the harm of shrimp seedling;Second is that water Matter is difficult to adjust, and the thick cultivation density of current mark is very big, generally can reach 30,000 tails water body per ton, and many farms are entire Culture-cycle is without changing water, so that water quality is easy to because the inaccuracy that feeds of bait causes the bad water such as ammonia nitrogen nitrous acid Prime factor it is exceeded, cause mark shrimp seedling survival rate it is low.On the other hand, it is slow to mark the thick stage shrimp seedling speed of growth.
Summary of the invention
The purpose of the invention is to overcome in the prior art the thick stage survival rate of South America prawn mark it is low, the speed of growth is slow The problem of, small size pond cultural method in a kind of thick stage room of Penaeus Vannmei mark is provided.
Above-mentioned purpose of the invention is to be achieved by following technical solution:
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps:
S1. container and water body disinfection, the water carrying capacity of container used are 0.1~0.5 ton;
S2. the disease-free shrimp seedling of health is chosen;
S3. mark extensive cultivation is grown: the culture-cycle is 20~30 days, and preceding 8 days breeding processes do not change water, every other day into container It splashes EM bacterium, carries out changing water after 8 days daily, change after water EM bacterium of splashing;First 8 days progress part shading treatments, certainly after 8 days It is cultivated under right light;In breeding process, fixed point feed for 12 times daily, feeds interval every time 90~120 minutes;It is grown in mark extensive cultivation 3~5 days before end, vitamin c solution of splashing daily carried out intensive treatment to shrimp seedling.
The mark for using water carrying capacity to carry out Penaeus Vannmei for 0.1~0.5 ton of small size pond is thick, close by reducing water body reduction Degree can greatly improve the survival rate in young shrimp mark thick stage, reduce the probability of morbidity, extensive large quantities of caused by reducing because of disease Measure the risk of young shrimp death.Small size pond cultivates the amount easier to control fed simultaneously, it is not easy to feed intake excessive or very few cause water body Pollution is conducive to improve efficiency of feed utilization, reduces ammonia nitrogen discharge, reduces environmental pollution.At first 8 days of raising, also due to young shrimp Very fragile feeding environment cannot have too big variation, because raisings in 8 days before this are not change water.And every one in this 8 days Its EM bacterium of splashing into container, can further suppress the growth of disinfect pathogen.Young shrimp grows to certain degree after 8 days, daily It carries out changing water and EM bacterium of splashing into container, raising water body ammonia-nitrogen content can be kept in lower level, it is also ensured that water Body does not generate disinfect pathogen.Young shrimp would not be increased in the mark thick stage by the infection of germ, survival rate.Strengthening process, daily It splashes vitamin c solution, the resistance germ ability of shrimp seedling can be further enhanced, guarantee survival rate.It will be in general aquaculture model Feed number daily and be changed to 12 times by 6 times, observe that shrimp seedling is again introduced into peak period of ingesting after feed 1.5~2 hours, It was fed every 1.5~2 hours so that food conversion and deposition greatly improve.It is thrown when shrimp seedling enters ingest peak period every time Hello, shrimp seedling is higher to the utilization rate of feed, and the speed of growth is faster.
Preferably, in the step S1, container bottom center is equipped with discharge outlet, and discharge outlet is equipped with 1 gas stone.
On cultivating system, the present invention by actual production test in most-often used 3 tons of water carrying capacity of circular fiber glass Bucket is changed to the circular glass fiber drum that water carrying capacity is 0.1~0.5 ton, and the side vent of round tank in production is changed in bottom Heart draining is accurately placed in bottom centre for gas stone from 8 becomes 1 in bucket in production.
Bottom centre sets drainage hole and drainpipe, and drainpipe is equipped with one, switch, for adjusting drainage speed.In drainage hole Place designs a kind of a kind of water bolt for combining embolus and sieve, in draining filtering walk impurities in water, residual bait, excrement etc. and Guarantee that shrimp seedling is not discharged.
Preferably, stocking density is 3500~4500 tail shrimp seedling per cubic meter of waters in step S3.
During selecting seedling, the young shrimp that the present invention chooses 5 days after becoming post larval from acanthosoma mark slightly, visually to see Examine, it is strong to choose appearance energy, place it in little Bai bowl and blown with mouth, can wave moves about against the current shrimp seedling, while observing shrimp seedling Enteron aisle and liver, have a complete gutstring so that enteron aisle is full, liver in black be it is excellent, the more unified the size specification of shrimp seedling the better.? From selecting at least 10 tails to carry out microscopy under microscope in shrimp seedling on the basis of visually observing, microscopy index includes helminth, muscle damage Hurt, appendage is clean and tidy etc..10 tail son shrimp of simultaneous selection carries out full shrimp vibrios measurement.
Selecting vibrios method for measuring during seedling is to take 5 tail shrimp seedlings, is respectively put into added with the homogenate of 1ml physiological saline Homogenate is coated on TCBS selection culture dish, the homogenate of every tail shrimp is done to uniform from 0.1ml is taken out in each homogenizer in device Three repetitions.All culture dishes are placed in 28 DEG C of cultures in constant incubator and for 24 hours, observe vibrios growing state, finally with culture Yellow bacterium number amount < 1000 on ware, green bacterium number amount < 10 are high-quality shrimp seedling, if bacterium number is excessive, should not select the young shrimp of this batch into Rower is thick.
Preferably, after the shrimp seedling is chosen, before shrimp seedling is put into Cultivation container, by shrimp seedling to be stocking be placed in containing It is impregnated 5~15 minutes in ascorbic solution, vitamin c solution, which is immersed in shrimp seedling body, can further increase supporting for shrimp seedling The ability of Anti-bacterium guarantees the survival rate of shrimp seedling.
Preferably, in the step S3, to four points that the quantity of exchanged water before strain is total Water after 8 days in breeding process One of, the quantity of exchanged water of last strain is the one third of total Water.As the growth of shrimp seedling is to the adaptation energy of environmental change Power is also stronger, increases quantity of exchanged water, it is ensured that raising water quality.
Preferably, in the step S3, the EM bacteria concentration splashed is 50~100 ppm.
Preferably, in the step S3, shrimp seedling intensive treatment, the concentration for vitamin c solution of splashing is 0.5~1 g/m3.
Preferably, in the step S3, daily timing 7:00 starts to feed.7 points of morning is that shrimp seedling is ingested after a night When amount is maximum, at this time starts to feed, the growth of shrimp seedling can be promoted.
It is highly preferred that the culturing time for marking the thick stage is 7:00;8:30;10:00;11:30;13:00;14:30;16:00; 17:30;19:00;20:30;22:00;23:30.
Preferably, in the step S3, the raw material of the feed fed includes: protein sources 69.75~79.47wt.% of raw material, Fat source 4~7wt.% of raw material, sugar source raw material 5wt.%, food binder 3wt.%, 0.3~1.2wt.% of antioxidant, phagostimulant 1.6wt.%, multi-vitamins 1wt.%, 1~4wt.% of composite mineral salt.
Preferably, the formula of the feed includes fish meal 52wt.%, protolysate comprising the feed fed 20wt.%, krill meal 3wt.%, 8.15~8.75wt.% of brewer's yeast 3wt.%, a- starch, lecithin 2wt.%, multi-vitamins 1wt.%, composite mineral salt 1wt.%, vitamin C phosphoric ester 0.65wt.%, methionine 1wt.%, tryptophan 0.5wt.%, lysine 1wt.%, choline chloride 1wt.%, sodium alginate 3wt.%, calcium dihydrogen phosphate 3wt.%.
Preferably, described to feed the citric acid that feed further includes 0.2~0.6 wt.%.Citric acid, lemon are added in feed Lemon acid changes the pH of shrimp body enteron aisle and vibrios is inhibited to grow nonparasitically upon another plant in enteron aisle, so as to improve the Anti-bacterium ability of shrimp seedling.
Generally when cultivating beginning, the feed fed is changed into 30 mesh after 10 days, is switched to after 16 days using 40 mesh screens 20 mesh, 1.2mm feed can directly be fed in 3~5 days before terminating by cultivating.
After mark extensive cultivation is grown, vibrios measurement is also carried out to shrimp seedling, method is to take 15 tail son shrimps, with 5 tail tail, one list Its liver is taken out in position, is put into the homogenizer added with 1ml physiological saline homogenate to uniform, dilutes homogenate in centrifuge tube It is coated on TCBS selection culture dish after 1000 times from taking-up 0.1ml in each centrifuge tube, the homogenate dilution of every tail shrimp does three A repetition.All culture dishes are placed in 28 DEG C of cultures in constant incubator and for 24 hours, vibrios growing state are observed, finally with culture dish Upper Huang bacterium number amount < 1000, green bacterium number amount < 10 are high-quality shrimp seedling, if bacterium number is excessive, the young shrimp of this batch should not be selected to carry out The pond culture in later period.
Mark extensive cultivation grow after, will also to shrimp seedling carry out salinity stress, method be take out 30 tail son shrimps be put into keg, will Seawater salinity by 35 ‰ is diluted to 6 ‰ with fresh water in bucket, and 6h is observed continuously, with final survival rate 50% the above are qualified young shrimp, The pond culture of next stage can be entered.
The present invention has following technical effect that compared with prior art
The present invention uses water carrying capacity to carry out the thick stage breeding of mark of South America prawn for 0.1~0.5 ton of small size pond, passes through contracting Small size pond cultivates the survival rate that can greatly improve the young shrimp mark thick stage, reduces the probability of morbidity, big caused by reducing because of disease The risk of the young shrimp death of scale high-volume.Small size pond cultivates the amount easier to control fed simultaneously, it is not easy to feed intake excessive or very few Water pollution is caused, is conducive to improve efficiency of feed utilization, ammonia nitrogen discharge is reduced, reduces environmental pollution.At first 8 days of raising, by There cannot be too big variation in the also very fragile feeding environment of young shrimp, because raisings in 8 days before this are not change water.And at this 8 days It inside every other day splashes into container EM bacterium, the growth of disinfect pathogen can be further suppressed.Young shrimp grows to certain journey after 8 days Degree, carries out changing water and EM bacterium of splashing into container daily, can keep raising water body ammonia-nitrogen content in lower level, can also be with Guarantee that water body does not generate disinfect pathogen.Young shrimp would not be increased in the mark thick stage by the infection of germ, survival rate.Strengthened Journey, vitamin c solution of splashing daily can further enhance the resistance germ ability of shrimp seedling, guarantee survival rate.It will generally cultivate Feeding number daily and be changed to 12 times by 6 times in mode observes that shrimp seedling is again introduced into height of ingesting after feed 1.5~2 hours Peak phase, interval are fed so that food conversion and deposition greatly improve for 1.5~2 hours.Enter peak period of ingesting every time in shrimp seedling Shi Jinhang is fed, and shrimp seedling is higher to the utilization rate of feed, and the speed of growth is faster.
Specific embodiment
The invention will be further described by the following examples, but does not limit the scope of the invention.In following embodiments Used test method is conventional method unless otherwise specified;Used material, reagent etc. are unless otherwise specified The reagent and material commercially obtained.
Rate of body weight gain described in each embodiment, specific growth rate and survival rate calculate as follows respectively:
Rate of body weight gain (%)=100 × (last weight-initial weight)/initial weight;
Survival rate (%)=(item number when item number/experiment at the end of experiment starts) × 100%.
Each embodiment feed formula of table 1 and Analysis of Nutritive Composition
Embodiment 1
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps:
S1. container and water body disinfection: before being put into shrimp seedling, first to capacity be 0.2 ton cylinder body and water body disappear Poison, cylinder body first use oxalic acid to impregnate scrub, inject fresh seawater after rinsing only, are carried out disinfection using strong chlorine oil, after standing 2 days Water body contents of residual chlorine is detected using chlorine residue kit, and safe concentration is adjusted to by sodium thiosulfate (dodium thiosulfate).
S2. choose the disease-free shrimp seedling of health: during selecting seedling, we are chosen after becoming post larval from acanthosoma 5 days Young shrimp mark thick, to visually observe, it is strong to choose appearance energy, places it in little Bai bowls and is blown with mouth, can wave against the current The shrimp seedling of travelling, while the enteron aisle and liver of shrimp seedling are observed, there is complete gutstring so that enteron aisle is full, liver is excellent, shrimp seedling in black Size specification it is more more unified better.On the basis of visually observing from selected in shrimp seedling at least 10 tails carry out microscope under microscopy, mirror Inspection index includes helminth, muscle damage, and appendage is clean and tidy etc..10 tail son shrimp of simultaneous selection carries out full shrimp vibrios measurement.In shrimp seedling Before putting seedling, by wait put shrimp seedling be placed in the keg containing Victoria C impregnate 15 minutes after be transferred in the thick cultivating system of mark.
S3. mark extensive cultivation is grown: the culture-cycle is 25 days, cultivates first 8 days without changing water, was changed daily at 9~21 days Water, quantity of exchanged water are the 1/4 of total Water, and the intensive treatment stage quantity of exchanged water of cultivation the 22nd~25 day is the 1/3 of total Water.It is whole simultaneously Concentration of every other day splashing into system for first 8 days in a culture-cycle is 50 ppm EM bacterium, is terminated changing water every time after 8 days Concentration of splashing in time afterwards is 50 ppm EM bacterium.In terms of environmental management, cultivating system is placed under identical illumination condition, preceding 8 It carries out part shading treatment to system, can carry out normal natural light cultivation after 8 days.Management aspect is fed, will be made to Feed carry out it is broken after fed using screen fractionation, daily feed for 12 times, time 7:00;8:30;10:00; 11:30;13:00;14:30;16:00;17:30;19:00;20:30;22:00;23:30.Later period strengthens aspect, terminates in cultivation Later period cultivates the 22nd~25 day, carry out intensive treatment, concentration of splashing daily 0.5g/m3 Victoria C solution to shrimp seedling.Mark the thick stage The feed fed is shown in Table the feed of the formula 1 in 1, and when feeding management, the feed of the partial size 1.2mm prepared is ground with grinder It after mill, is classified with sieve, cultivation fed 40 mesh screen screenings (200~400um) in 1~5 day, fed within 6-10 days 40 mesh Oversize (400um) feeds 30 mesh oversizes (600um) for 11~15 days, feeds within 16~20 days 20 mesh oversizes (800um), and 21 Feed 1.2mm feed within~25 days.Control treatment is done simultaneously, control treatment is using traditional thick cultural method+traditional commerce of mark Micro-granulated feed nursing carries out mark extensive cultivation and grows.
In the identical situation of original body mass (original body mass 2.3mg), aquaculture model of the present invention and general biography The aquaculture model (control) of system farm is compared, and terminal weight, which improves 38%(mode of the present invention and cultivates to obtain young shrimp, to be averaged opisthosoma Weight is 0.207g, and the opisthosoma weight that business micro-granulated feed is fed is 0.15g), rate of body weight gain (WG) improves 21.7%(present invention feeding The WG that material is fed is 9003.90, and the WG that commercial feed is fed is that 7400), survival rate improves 55.7%(forage feed of the present invention Survival rate is 93.4%, 60%) survival rate that business micro-granulated feed is fed is.
Under cultivation mid-term, the tradition aquaculture model of measurement in continuous seven days and small size pond aquaculture model is lauched middle ammonia nitrogen and nitrous The concentration of hydrochlorate.By comparison, it was found that range of the general concentration of ammonia nitrogen in 0.3~0.55mg/L, and small size pond under traditional mode For ammonia nitrogen concentration between 0.1~0.28mg/L, the latter is significantly lower than the former under mode.Nitrite concentration under traditional mode simultaneously Nitrite concentration is within the scope of 0.01~0.04mg/L at 0.17~0.28mg/L, small size pond mode, it is seen that relative to biography System mode, small size pond mode have a great superiority on ammonia nitrogen, the control of nitrite, water quality indicator it is superior also further Explain the reason of survival rate increases.
Embodiment 2
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps: cultural method is same Embodiment 1 cultivates the feed that the feed used is formula 2 in table 1.The identical control group of same embodiment 1 is set up simultaneously.
In the identical situation of original body mass (original body mass 2.3mg), aquaculture model of the present invention and general biography The aquaculture model (control) of system farm is compared, and rate of body weight gain improves 23.3%, and survival rate improves 49%(forage feed of the present invention WG be 9125.5, commercial feed feed WG be 7400;The survival rate of forage feed of the present invention is 89.4%, business microparticle 60%) survival rate of forage feed is.
Under cultivation mid-term, the tradition aquaculture model of measurement in continuous seven days and small size pond aquaculture model is lauched middle ammonia nitrogen and nitrous The concentration of hydrochlorate.By comparison, it was found that range of the general concentration of ammonia nitrogen in 0.3~0.55mg/L, and small size pond under traditional mode For ammonia nitrogen concentration between 0.1~0.28mg/L, the latter is significantly lower than the former under mode.Nitrite concentration under traditional mode simultaneously Nitrite concentration is within the scope of 0.01~0.04mg/L at 0.17~0.28mg/L, small size pond mode, it is seen that relative to biography System mode, small size pond mode have a great superiority on ammonia nitrogen, the control of nitrite, water quality indicator it is superior also further Explain the reason of survival rate increases.
Embodiment 3
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps: cultural method is same Embodiment 1 cultivates the feed that the feed used is formula 3 in table 1.The identical control group of same embodiment 1 is set up simultaneously.
In the identical situation of original body mass (original body mass 2.3mg), aquaculture model of the present invention and general biography The aquaculture model (control) of system farm is compared, and rate of body weight gain improves 25.9%, and survival rate improves 48.25% (feed of the present invention The WG of nursing is 9318.2, and the WG that commercial feed is fed is 7400;The survival rate of forage feed of the present invention is 88.95%, and business is micro- 60%) survival rate that pellet is fed is.
Under cultivation mid-term, the tradition aquaculture model of measurement in continuous seven days and small size pond aquaculture model is lauched middle ammonia nitrogen and nitrous The concentration of hydrochlorate.By comparison, it was found that range of the general concentration of ammonia nitrogen in 0.3~0.55mg/L, and small size pond under traditional mode For ammonia nitrogen concentration between 0.1~0.28mg/L, the latter is significantly lower than the former under mode.Nitrite concentration under traditional mode simultaneously Nitrite concentration is within the scope of 0.01~0.04mg/L at 0.17~0.28mg/L, small size pond mode, it is seen that relative to biography System mode, small size pond mode have a great superiority on ammonia nitrogen, the control of nitrite, water quality indicator it is superior also further Explain the reason of survival rate increases.
Embodiment 4
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps: cultural method is same Embodiment 1 cultivates the feed that the feed used is formula 4 in table 1.The identical control group of same embodiment 1 is set up simultaneously.
In the identical situation of original body mass (original body mass 2.3mg), aquaculture model of the present invention and general biography The aquaculture model (control) of system farm is compared, and rate of body weight gain improves 10.6%, and survival rate improves 37.9%, and (feed of the present invention is fed Feeding WG is 8183.0, and the WG that commercial feed is fed is 7400;The survival rate of forage feed of the present invention is 82.75%, micro- of business 60%) survival rate of grain forage feed is.
Under cultivation mid-term, the tradition aquaculture model of measurement in continuous seven days and small size pond aquaculture model is lauched middle ammonia nitrogen and nitrous The concentration of hydrochlorate.By comparison, it was found that range of the general concentration of ammonia nitrogen in 0.3~0.55mg/L, and small size pond under traditional mode For ammonia nitrogen concentration between 0.1~0.28mg/L, the latter is significantly lower than the former under mode.Nitrite concentration under traditional mode simultaneously Nitrite concentration is within the scope of 0.01~0.04mg/L at 0.17~0.28mg/L, small size pond mode, it is seen that relative to biography System mode, small size pond mode have a great superiority on ammonia nitrogen, the control of nitrite, water quality indicator it is superior also further Explain the reason of survival rate increases.
By the result of Examples 1 to 4 it is found that being compared with traditional aquaculture model, small size pond indoor culture of the invention It is significantly improved in terms of rate of body weight gain and survival rate, while under identical aquaculture model, 0.4% citric acid is added in feed can be with It significantly improves the rate of body weight gain of young shrimp and does not have a significant impact to survival rate.
Embodiment 5
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps:
S1. container and water body disinfection: before being put into shrimp seedling, first to capacity be 0.1 ton cylinder body and water body disappear Poison, cylinder body first use oxalic acid to impregnate scrub, inject fresh seawater after rinsing only, are carried out disinfection using strong chlorine oil, after standing 2 days Water body contents of residual chlorine is detected using chlorine residue kit, and safe concentration is adjusted to by sodium thiosulfate (dodium thiosulfate).
S2. choose the disease-free shrimp seedling of health: during selecting seedling, we are chosen after becoming post larval from acanthosoma 5 days Young shrimp mark thick, to visually observe, it is strong to choose appearance energy, places it in little Bai bowls and is blown with mouth, can wave against the current The shrimp seedling of travelling, while the enteron aisle and liver of shrimp seedling are observed, there is complete gutstring so that enteron aisle is full, liver is excellent, shrimp seedling in black Size specification it is more more unified better.On the basis of visually observing from selected in shrimp seedling at least 10 tails carry out microscope under microscopy, mirror Inspection index includes helminth, muscle damage, and appendage is clean and tidy etc..10 tail son shrimp of simultaneous selection carries out full shrimp vibrios measurement.In shrimp seedling Before putting seedling, shrimp seedling to be put is placed in the keg containing Victoria C to impregnate to be transferred to after five minutes and is marked in thick cultivating system.
S3. mark extensive cultivation is grown: first 8 days that the culture-cycle is 20 days without changing water, being carried out changing water at 9~17 days, is changed water Amount is the 1/4 of total Water, is the 1/3 of total Water in the intensive treatment stage quantity of exchanged water of cultivation the 18th~20 day.It is entire simultaneously to support Growing concentration of every other day splashing into system for first 8 days in the period is 100 ppm EM bacterium, after 8 days after changing water every time and When splash concentration be 100 ppm EM bacterium.In terms of environmental management, cultivating system is placed under identical illumination condition, at first 8 days Part shading treatment is carried out to system, normal natural light cultivation can be carried out after 8 days.Management aspect is fed, it will be according in table 1 The feed that is made to of formula 3 carry out it is broken after fed using screen fractionation, daily feed for 12 times, the time 10: 00;11:30;13:00;14:30;16:00;17:30;18:00;19:30;20:00;21:30;23:00;1:30.Later period strengthens Aspect terminates the later period in cultivation, that is, cultivates the 18th~20 day, carries out intensive treatment, Victoria C solution concentration of splashing daily to shrimp seedling 1g/m³。
Embodiment 6
Efficiently in small water body cultural method in a kind of thick stage room of Penaeus Vannmei mark, comprising the following steps:
S1. container and water body disinfection: before being put into shrimp seedling, first to capacity be 0.5 ton cylinder body and water body disappear Poison, cylinder body first use oxalic acid to impregnate scrub, inject fresh seawater after rinsing only, are carried out disinfection using strong chlorine oil, after standing 2 days Water body contents of residual chlorine is detected using chlorine residue kit, and safe concentration is adjusted to by sodium thiosulfate (dodium thiosulfate).
S2. choose the disease-free shrimp seedling of health: during selecting seedling, we are chosen after becoming post larval from acanthosoma 5 days Young shrimp mark thick, to visually observe, it is strong to choose appearance energy, places it in little Bai bowls and is blown with mouth, can wave against the current The shrimp seedling of travelling, while the enteron aisle and liver of shrimp seedling are observed, there is complete gutstring so that enteron aisle is full, liver is excellent, shrimp seedling in black Size specification it is more more unified better.On the basis of visually observing from selected in shrimp seedling at least 10 tails carry out microscope under microscopy, mirror Inspection index includes helminth, muscle damage, and appendage is clean and tidy etc..10 tail son shrimp of simultaneous selection carries out full shrimp vibrios measurement.In shrimp seedling Before putting seedling, by wait put shrimp seedling be placed in the keg containing Victoria C impregnate 8 minutes after be transferred in the thick cultivating system of mark.
S3. mark extensive cultivation is grown: first 8 days that the culture-cycle is 30 days without changing water, carried out changing water daily at 9~25 days, Quantity of exchanged water is the 1/4 of total Water, is the 1/3 of total Water in the intensive treatment stage quantity of exchanged water of cultivation the 18th~20 day.It is whole simultaneously Concentration of every other day splashing into system for first 8 days in a culture-cycle is 80 ppm EM bacterium, is terminated changing water every time after 8 days Concentration of splashing in time afterwards is 80 ppm EM bacterium.In terms of environmental management, cultivating system is placed under identical illumination condition, Part shading treatment is carried out to system at first 8 days, normal natural light cultivation can be carried out after 8 days.Management aspect is fed, it will be by The feed that formula 3 is made to fed after being crushed using screen fractionation, daily feed for 12 times, time 6:00; 8:00;10:00;12:00;14:00;16:00;18:00;20:00;22:00;24:00;2:00;4:00.Later period strengthens aspect, Terminate the later period in cultivation, that is, cultivate the 26th~30 day, intensive treatment, Victoria C solution concentration of splashing daily 1g/m3 are carried out to shrimp seedling.

Claims (8)

1. small size pond cultural method in a kind of thick stage room of Penaeus Vannmei mark, which comprises the following steps:
S1. container and water body disinfection, the small size pond that the water carrying capacity of container used is 0.1~0.5 ton;
S2. the disease-free shrimp seedling of health is chosen;
S3. mark extensive cultivation is grown: the culture-cycle is 20~30 days, and preceding 8 days breeding processes do not change water, every other day splash into container EM bacterium carries out changing water after 8 days daily, changes after water EM bacterium of splashing;First 8 days progress part shading treatments, in natural light after 8 days Lower cultivation;In breeding process, fixed point feed for 12 times daily, feeds interval every time 90~120 minutes;End is grown in mark extensive cultivation Preceding 3~5 days, vitamin c solution of splashing daily carry out intensive treatment to shrimp seedling;
Wherein, the feed fed include fish meal 52wt.%, protolysate 20wt.%, krill meal 3wt.%, brewer's yeast 3wt.%, 8.15~8.75wt.% of a- starch, lecithin 2wt.%, multi-vitamins 1wt.%, composite mineral salt 1wt.%, vitamin C phosphoric acid Ester 0.65wt.%, methionine 1wt.%, tryptophan 0.5wt.%, lysine 1wt.%, choline chloride 1wt.%, sodium alginate 3wt.%, Calcium dihydrogen phosphate 3wt.%.
2. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 1, which is characterized in that institute in S1 Container bottom center is stated equipped with discharge outlet, discharge outlet is equipped with 1 gas stone.
3. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 1, which is characterized in that in S2 After shrimp seedling is chosen, shrimp seedling to be stocking is placed in containing impregnating 5~15 minutes in ascorbic solution.
4. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 1, which is characterized in that the step It is a quarter of total Water to the quantity of exchanged water before intensive treatment in rapid S3, after cultivation 8 days, the quantity of exchanged water in intensive treatment stage is The one third of total Water.
5. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 1, which is characterized in that the step In rapid S3, the EM bacteria concentration splashed is 50~100ppm.
6. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 1, which is characterized in that the step In rapid S3, shrimp seedling intensive treatment, the concentration for vitamin c solution of splashing is 0.5~1g/m3.
7. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 1, which is characterized in that the step In rapid S3, daily 7:00 timing starts to feed.
8. small size pond cultural method in the thick stage room of Penaeus Vannmei mark according to claim 7, which is characterized in that the throwing The feed fed further includes the citric acid of 0.2~0.6 wt.%.
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