CN108751272A - A kind of method of acid processing Enteromorpha under low-salt conditions - Google Patents
A kind of method of acid processing Enteromorpha under low-salt conditions Download PDFInfo
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- CN108751272A CN108751272A CN201810478135.7A CN201810478135A CN108751272A CN 108751272 A CN108751272 A CN 108751272A CN 201810478135 A CN201810478135 A CN 201810478135A CN 108751272 A CN108751272 A CN 108751272A
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- enteromorpha
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
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Abstract
The invention belongs to marine organisms field, the method that relates in particular to acid processing Enteromorpha under a kind of low-salt conditions.Enteromorpha frond is placed in acid solution and is impregnated, realizes the processing to Enteromorpha on solid matrix.Judge that growth conditions, analyzing processing effect enable Enteromorpha to remove by photochemical vitality.The present invention is easy to operate, and method is effective, can remove the Enteromorpha in solid matrix, slows down its proliferative speed.
Description
Technical field
The invention belongs to marine organisms field, the method that relates in particular to acid processing Enteromorpha under a kind of low-salt conditions.
Background technology
So far from 2007, connected in China's Yellow Sea with the green tide that Enteromorpha (Ulva prolifera) is main reason kind
Continuous periodically outburst for many years, causes serious environmental disruption and economic loss, causes the extensive concern of society.Enteromorpha reproduction
Mode is various, and wherein sporogony is a kind of its important modes of reproduction.Enteromorpha spore can synthesize and discharge a kind of gathering for stickiness
Close glycoprotein, will adhere to rapidly when encountering suitable attachment base, under suitable nutrition and environmental condition, carry out sprout and it is fast
Fast-growing is long.Suitable attachment base includes marine product (algae and shellfish etc.) culture raft frame (hawser, bamboo pole etc.), sand grain, glass
Glass and plastics etc..Research Enteromorpha adjusting and controlling growth and the removing of Enteromorpha in attachment base have great significance for green alga control.
The Enteromorpha speed of growth is fast, and environment wide adaptation range, resistance is strong, to do dehydration, salinity, temperature etc. all have compared with
Strong adaptability.It is for example " dry dew " etc., bad to the elimination effect of Enteromorpha suitable for the sweep-out method of other miscellaneous algaes.How
Simply and effectively remove the Enteromorpha in attachment base, it has also become restrict the problem of production and control algae tide formation.
Invention content
The purpose of the present invention is to provide a kind of methods of acid processing Enteromorpha under low-salt conditions.
To achieve the above object, the invention adopts a technical scheme as：
The method of acid processing Enteromorpha, Enteromorpha frond is placed in acid solution and is impregnated, is realized to solid under a kind of low-salt conditions
The processing of Enteromorpha in matrix.
By measuring photochemical vitality after the immersion, judges Enteromorpha growth conditions, analyze to Enteromorpha on solid matrix
Treatment effect.
The pH of the acid solution is 4.0；Salinity is 0 ‰ -90 ‰；Soaking time is 1h-6h.
The Enteromorpha frond be placed in salinity be 0 ‰, 15 ‰, 60 ‰ or 90 ‰ in.
The Enteromorpha frond is immersed in the acid solution of 0 ‰ salinity, and the immersion treatment time is 1h；
In the acid solution of 15 ‰ salinity, the immersion treatment time is 1h-3h；
In the acid solution of 60 ‰, 90 ‰ salinity, the immersion treatment time is 1h-6h.
The acid is hydrochloric acid, sulfuric acid, phosphoric acid or citric acid.
It is as follows：
1. configuring the acid solution of different salinity (0 ‰, 15 ‰, 60 ‰, 90 ‰).
2. the good Enteromorpha frond of growth conditions is thrown in different acid solutions, photosynthetic work is measured using Dual-PAM
Use parameter.
Enteromorpha is placed in the acid solution of different salinity and handles by the present invention.Under low-salt conditions (0 ‰, 15 ‰), add
Sour processing group frond photosynthetic activity decline than normal salinity (30 ‰) acid processing it is notable, after handling 1h, YII values be down to 0.10 with
Under；Under high salt conditions (60 ‰, 90 ‰), acid adding processing group frond photosynthetic activity and the no larger difference of seawater acid processing group,
With the increase of processing time, acid adding processing group photosynthetic activity with high salt is higher instead.Show that acid processing is to Enteromorpha under low-salt conditions
Photosynthetic activity is affected, and it is with high salt seem to acid processing have certain protective effect.
The invention has the advantages that：
The present invention handles Enteromorpha frond using the acid solution of different salinity, it is found that acid processing is to frond under low-salt conditions
It is affected, the removing of Enteromorpha in matrix can be carried out with the method；Specially：
1. cost-effective, acid solution used is prepared with fresh water.
2. operating process is simple, the solid matrix with miscellaneous algae Enteromorpha need to be only immersed in acid molten in practical operation of the present invention
A period of time in liquid.
3. selection is wide, sour range of choice is wider in process of the present invention, hydrochloric acid, sulfuric acid, phosphoric acid and citric acid.
Description of the drawings
Fig. 1 is Enteromorpha provided in an embodiment of the present invention respectively through fresh water (0 ‰ salinity), 4.0 hydrochloric acid solutions of pH (seawater acid
Solution) and 4.0 fresh water solution of pH (fresh water acid solution) processing 1h after Fv/Fm variation diagram.
Fig. 2 is Enteromorpha provided in an embodiment of the present invention respectively through fresh water (0 ‰ salinity), 4.0 hydrochloric acid solutions of pH (seawater acid
Solution) and 4.0 fresh water solution of pH (fresh water acid solution) processing 1h after YI and YII variation diagram.
Fig. 3 is Enteromorpha provided in an embodiment of the present invention respectively through seawater：Fresh water=1:1 solution (15 ‰ salinity), pH 4.0
Hydrochloric acid solution (seawater acid solution) and 15 ‰ salinity pH, 4.0 hydrochloric acid solutions handle the variation diagram of Fv/Fm after 1h.
Fig. 4 is Enteromorpha provided in an embodiment of the present invention respectively through seawater：Fresh water=1:1 solution (15 ‰ salinity), pH 4.0
Hydrochloric acid solution (seawater acid solution) and 15 ‰ salinity pH, 4.0 hydrochloric acid solutions handle the variation diagram of YI and YII after 1h.
Fig. 5 is Enteromorpha provided in an embodiment of the present invention respectively through seawater：Fresh water=1:1 solution (15 ‰ salinity), pH 4.0
Hydrochloric acid solution (seawater acid solution) and 15 ‰ salinity pH, 4.0 hydrochloric acid solutions handle the variation diagram of Fv/Fm after 3h.
Fig. 6 is Enteromorpha provided in an embodiment of the present invention respectively through seawater：Fresh water=1:1 solution (15 ‰ salinity), pH 4.0
Hydrochloric acid solution (seawater acid solution) and 15 ‰ salinity pH, 4.0 hydrochloric acid solutions handle the variation diagram of YI and YII after 3h.
Fig. 7 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (60 ‰ salinity), 4.0 hydrochloric acid solutions of pH (sea
Water acid solution) and 60 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 1h after Fv/Fm variation diagram.
Fig. 8 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (60 ‰ salinity), 4.0 hydrochloric acid solutions of pH (sea
Water acid solution) and 60 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 1h after YI and YII variation diagram.
Fig. 9 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (60 ‰ salinity), 4.0 hydrochloric acid solutions of pH (sea
Water acid solution) and 60 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 3h after Fv/Fm variation diagram.
Figure 10 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (60 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 60 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 3h after YI and YII variation diagram.
Figure 11 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (60 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 60 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 6h after Fv/Fm variation diagram.
Figure 12 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (60 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 60 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 6h after YI and YII variation diagram.
Figure 13 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (90 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 90 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 1h after Fv/Fm variation diagram.
Figure 14 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (90 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 90 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 1h after YI and YII variation diagram.
Figure 15 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (90 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 90 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 3h after Fv/Fm variation diagram.
Figure 16 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (90 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 90 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 3h after YI and YII variation diagram.
Figure 17 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (90 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 90 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 6h after Fv/Fm variation diagram.
Figure 18 is Enteromorpha provided in an embodiment of the present invention respectively through high sea brine (90 ‰ salinity), pH4.0 hydrochloric acid solutions (sea
Water acid solution) and 90 ‰ salinity pH, 4.0 hydrochloric acid solutions processing 6h after YI and YII variation diagram.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after having read present disclosure, those skilled in the art can
To be made various changes or modifications to the present invention, such equivalent forms equally fall within limited range of the present invention.
Embodiment 1,
Treatment conditions：Solution (fresh water) that salinity is 0 ‰, the hydrochloric acid solution (seawater acid solution) that pH is 4.0, pH 4.0
Fresh water solution (fresh water acid solution) handle Enteromorpha
1. adding concentrated hydrochloric acid in nature seawater, it is 4.0 to adjust pH with pH meter, prepares seawater acid solution；Add in fresh water
Enriching hydrochloric acid, it is 4.0 to adjust pH with pH meter, prepares fresh water acid solution.
2. by the good Enteromorpha of growth conditions be respectively placed in seawater (control group) in the treatment conditions of above-mentioned setting, fresh water,
Seawater acid solution, fresh water acid solution handle 1h respectively, and photosynthetic parameters are measured using Dual-PAM.It was found that at the fresh water short time
Reason has no effect on the photosynthetic activity of Enteromorpha；The processing of seawater acid can influence the photosynthetic parameters of Enteromorpha, and wherein Fv/Fm is down to by 0.89
About 0.77, YII is down to 0.40 or so by 0.80 or so；Influence of the fresh water acid processing to Enteromorpha photosynthetic activity is more notable, Fv/Fm
It is down to about 0.25, YII and is down to 0.10 or less (referring to Fig. 1 and 2).
By above-mentioned Fig. 1 and 2 as it can be seen that acid processing can significantly reduce the photosynthetic activity of Enteromorpha frond, salinity (fresh water) is reduced
Acid processing can more significantly affect its photosynthetic activity.
Embodiment 2,
Treatment conditions：Seawater：Fresh water=1:1 (v/v) solution (15 ‰ salinity), (seawater acid is molten for the hydrochloric acid solution that pH is 4.0
Liquid), 15 ‰ salinity pH, 4.0 hydrochloric acid solutions handle Enteromorpha
1. preparing seawater：Fresh water=1:1 (v/v) solution；Concentrated hydrochloric acid is added in nature seawater, adjusting pH with pH meter is
4.0, prepare seawater acid solution；In seawater：Fresh water=1:Concentrated hydrochloric acid is added in 1 solution, it is 4.0 to adjust pH with pH meter, is prepared
15 ‰ salinity pH, 4.0 hydrochloric acid solutions.
2. the good Enteromorpha of growth conditions is respectively placed in above-mentioned treatment conditions, at the same with ordinary sea water (control group),
15 ‰ salinity solution, seawater acid solution and 15 ‰ salinity pH, 4.0 hydrochloric acid solutions handle 1h, 3h respectively, are measured using Dual-PAM
Photosynthetic parameters.It was found that after processing 1h, the photosynthetic activity of each processing group frond is all remarkably decreased compared with the control group, by decline
Degree sequence is followed successively by：15 ‰ salinity solution, seawater acid solution and 15 ‰ salinity acid solutions (ginseng is seen figures 3 and 4).When with processing
Between increase (3h), each group photosynthetic parameters numerical value continues to decline, wherein the Enteromorpha Fv/Fm of 15 ‰ salinity acid solutions processing is reduced to
0.10 hereinafter, YI and YII are reduced to 0 (referring to Figures 5 and 6).
By above-mentioned Fig. 3-6 as it can be seen that for seawater acid solution processing group, compared with Low-salinity acid solution to Enteromorpha frond
Photosynthetic activity is affected.
Embodiment 3,
Treatment conditions：High sea brine (60 ‰ salinity), the hydrochloric acid solution (seawater acid solution) that pH is 4.0,60 ‰ salinity pH
4.0 hydrochloric acid solutions handle Enteromorpha
1. adding sodium chloride in nature seawater, it is 60 ‰ to adjust salinity with salinometer；Dense salt is added in nature seawater
Acid, it is 4.0 to adjust pH with pH meter, prepares seawater acid solution；Concentrated hydrochloric acid is added in 60 ‰ salinity solution, adjusting pH with pH meter is
4.0, prepare 60 ‰ salinity pH, 4.0 hydrochloric acid solutions.
2. by the good Enteromorpha of growth conditions be respectively placed in 60 ‰ salinity solution in above-mentioned treatment conditions, seawater acid solution,
1h, 3h, 6h are handled respectively in 60 ‰ salinity pH, 4.0 hydrochloric acid solutions, and photosynthetic parameters are measured using Dual-PAM.It was found that place
After managing 1h, Seawater Treatment group with high salt changes without conspicuousness compared with the control group；Seawater acid solution and high hydrochloric acid solution processing group
Frond photosynthetic activity is remarkably decreased, but this two groups do not have larger gap (Fig. 7 and 8).Handle 3h after, seawater acid solution with it is with high salt
Acid solution processing group frond photosynthetic activity continues to decline, and ironically, high hydrochloric acid solution processing group is compared to seawater acid solution
Processing group declines slowly (Fig. 9 and 10).After handling 6h, seawater acid solution is down to high hydrochloric acid solution processing group frond photosynthetic activity
Almost 0 (Figure 11 and 12).
By above-mentioned Fig. 7-12 as it can be seen that for seawater acid solution processing group, the acid processing under high salt conditions is for waterside
Tongue frond has certain protective effect.
Embodiment 4,
Treatment conditions：High sea brine (90 ‰ salinity), the hydrochloric acid solution (seawater acid solution) that pH is 4.0,90 ‰ salinity pH
4.0 hydrochloric acid solutions handle Enteromorpha
1. adding sodium chloride in nature seawater, it is 90 ‰ to adjust salinity with salinometer；Dense salt is added in nature seawater
Acid, it is 4.0 to adjust pH with pH meter, prepares seawater acid solution；Concentrated hydrochloric acid is added in 90 ‰ salinity solution, adjusting pH with pH meter is
4.0, prepare 90 ‰ salinity pH, 4.0 hydrochloric acid solutions.
2. by the good Enteromorpha of growth conditions be respectively placed in 90 ‰ salinity solution in above-mentioned treatment conditions, seawater acid solution,
1h, 3h, 6h are handled respectively in 90 ‰ salinity pH, 4.0 hydrochloric acid solutions, and photosynthetic parameters are measured using Dual-PAM.It was found that place
After managing 1h, each processing group has significant decline relative to control group, wherein under seawater acid solution and high hydrochloric acid solution processing group
Drop becomes apparent (Figure 13 and 14).After handling 3h, the frond photosynthetic activity of high hydrochloric acid solution processing group is molten higher than seawater acid instead
Liquid processing group (Figure 15 and 16).After handling 6h, seawater acid solution processing group frond photosynthetic activity is down to almost 0, and high hydrochloric acid is molten
Liquid processing group frond photosynthetic activity has certain value, although relatively low (Figure 17 and 18).
By above-mentioned Figure 13-18 as it can be seen that for seawater acid solution processing group, the acid processing under high salt conditions is for waterside
The influence of tongue frond is big not as good as low HCl treatment, shows with high salt with certain protective effect.
The present invention handles Enteromorpha using the acid solution of different salinity, it is found that acid processing influences bigger under low-salt conditions,
In handled with fresh water acid solution and influence maximum, have certain protective effect instead to acid processing under high salt conditions.
Keep this field special by the foregoing description of the disclosed embodiments for the description to the embodiment of the present invention above
Industry technical staff can realize or use the present invention.Various modifications to these embodiments carry out those skilled in the art
It says and will be apparent, the general principles defined herein can be the case where not departing from the spirit or scope of the present invention
Under, it realizes in other embodiments.Therefore, the present invention is not intended to be limited to the embodiments shown herein, but to accord with
Close widest range consistent with the principles and novel features disclosed in this article.
Claims (6)
1. a kind of method of acid processing Enteromorpha under low-salt conditions, it is characterised in that：Enteromorpha frond is placed in acid solution and is impregnated, it is real
Now to the processing of Enteromorpha on solid matrix.
2. the method for acid processing Enteromorpha under low-salt conditions as described in claim 1, it is characterised in that：Pass through survey after the immersion
Determine photochemical vitality, judge Enteromorpha growth conditions, analyzes the treatment effect to Enteromorpha on solid matrix.
3. the method for acid processing Enteromorpha under low-salt conditions as described in claim 1, it is characterised in that：The pH of the acid solution is
4.0；Salinity is 0 ‰ -90 ‰；Soaking time is 1h-6h.
4. the method for acid processing Enteromorpha under low-salt conditions as described in claim 3, it is characterised in that：The Enteromorpha frond is placed in
During salinity is 0 ‰, 15 ‰, 60 ‰ or 90 ‰.
5. the method for acid processing Enteromorpha under low-salt conditions as described in claim 4, it is characterised in that：The Enteromorpha frond impregnates
In the acid solution of 0 ‰ salinity, the immersion treatment time is 1h；
In the acid solution of 15 ‰ salinity, the immersion treatment time is 1h-3h；
In the acid solution of 60 ‰, 90 ‰ salinity, the immersion treatment time is 1h-6h.
6. the method for acid processing Enteromorpha under low-salt conditions as described in claim 4, it is characterised in that：The acid is hydrochloric acid, sulphur
Acid, phosphoric acid or citric acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113925058A (en) * | 2021-11-01 | 2022-01-14 | 上海海洋大学 | Mixed acid treatment solution for removing sessile green algae on porphyra yezoensis cultivation raft and method for removing green algae by using mixed acid treatment solution |
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| CN103005151A (en) * | 2012-12-05 | 2013-04-03 | 青岛艾华隆生物科技有限公司 | Method for preparing free enteromorpha amino acid by using acid hydrolysis method |
| CN103609427A (en) * | 2013-12-02 | 2014-03-05 | 连云港市海洋与水产科学研究所 | Acid treatment type full-floating laver culture method |
| CN107262439A (en) * | 2017-06-28 | 2017-10-20 | 中国科学院海洋研究所 | A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101822271A (en) * | 2010-05-11 | 2010-09-08 | 宁波大学 | Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof |
| CN103005151A (en) * | 2012-12-05 | 2013-04-03 | 青岛艾华隆生物科技有限公司 | Method for preparing free enteromorpha amino acid by using acid hydrolysis method |
| CN103609427A (en) * | 2013-12-02 | 2014-03-05 | 连云港市海洋与水产科学研究所 | Acid treatment type full-floating laver culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113925058A (en) * | 2021-11-01 | 2022-01-14 | 上海海洋大学 | Mixed acid treatment solution for removing sessile green algae on porphyra yezoensis cultivation raft and method for removing green algae by using mixed acid treatment solution |
| CN113925058B (en) * | 2021-11-01 | 2023-09-08 | 上海海洋大学 | Mixed acid treatment solution for removing green algae fixed on Porphyra yezoensis cultivation raft frame and method for removing green algae |
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