CN107262439A - A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining - Google Patents
A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining Download PDFInfo
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- CN107262439A CN107262439A CN201710507386.9A CN201710507386A CN107262439A CN 107262439 A CN107262439 A CN 107262439A CN 201710507386 A CN201710507386 A CN 201710507386A CN 107262439 A CN107262439 A CN 107262439A
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- enteromorpha
- porphyra yezoensis
- lace curtaining
- acid solution
- acid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to marine organisms field, a kind of method that lace curtaining supernatant removal of impurities algae Enteromorpha is cultivated from Porphyra yezoensis is related in particular to.The Porphyra yezoensis lace curtaining that has miscellaneous algae Enteromorpha of growing nonparasitically upon another plant, which is placed in acid solution, to be soaked, and is placed in nature seawater and is recovered afterwards, and then Enteromorpha is removed (death), and does not influence Porphyra yezoensis to grow.The present invention is simple to operate, and method effectively, can improve the yield and quality of Porphyra yezoensis, improve the economic benefit of seaweed industry.
Description
Technical field
The invention belongs to marine organisms field, relate in particular to a kind of Porphyra yezoensis of removing and cultivate miscellaneous algae Enteromorpha on lace curtaining
Method.
Background technology
Seaweed has important nutritive value and economic value, cultivates extensively in the coastal various countries in Southeast Asia.The world in 2013
Seaweed yield is about 1,800,000 tons, and the output value is up to 1,200,000,000 dollars (FAO).China's seaweed industry development is rapid, according to 2016 China Fisheries
The data that statistical yearbook is announced, China's laver culture area in 2016 is 65766 hectares, and cultured output is 115875 tons.The Changjiang river
To the north of based on Porphyra yezoensis, be concentrated mainly on Jiangsu Province and Shandong Province.Wherein about 330,000 mu of Jiangsu Province seaweed cultivated area, from
Industry personnel 80,000 or so, and the main producing region of China's Porphyra yezoensis, the industry gross output value 20 are formd in Nantong, Yancheng and Lianyun Harbour sea area
Hundred million yuan or so, be the important component that China's export is earned foreign exchange.
A variety of miscellaneous algaes that grow nonparasitically upon another plant are easy in seaweed cultivation, on lace curtaining, the wherein harm of Enteromorpha is maximum.In seaweed children
Seedling stage, if Enteromorpha is grown nonparasitically upon another plant will have a strong impact on the growth of seaweed seedling in lace curtaining;In the middle and later periods of cultivation, the meeting of growing nonparasitically upon another plant of Enteromorpha
Influence yield, harvest and the quality of seaweed.Therefore, having carried out technology and the methods such as " cold net ", " dry dew ", kill purple with streak
Dish has the miscellaneous algae of competitive relation, but excessive (time, human cost etc.) due to being restricted factor, and progress is relatively slow.Such as
What simplicity, economically removing are attached to the miscellaneous algae on seaweed lace curtaining, it has also become the problem of restriction Porphyra yezoensis cultivation.
The content of the invention
It is an object of the invention to provide a kind of method for removing miscellaneous algae Enteromorpha on Porphyra yezoensis cultivation lace curtaining.
To achieve the above object, the present invention use technical scheme for:
A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining, the Porphyra yezoensis lace curtaining that has miscellaneous algae Enteromorpha of growing nonparasitically upon another plant is placed in acid
Soaked in solution, be placed in nature seawater and recover again afterwards, Enteromorpha is removed, and the Porphyra yezoensis on lace curtaining is given birth to
It is long, the photochemical vitality change of frond is determined after restoring sea water, concentration and influence of the time to frond of acid treatment is analyzed,
And then miscellaneous algae Enteromorpha on acid treated lace curtaining can be enable to remove.
The pH of the acid solution is 1.0-2.0;Soak time is 30s-10min.
The pH of the acid solution is 1.0 or 2.0.
Described to be immersed in the acid solution that pH is 1.0, the immersion treatment time is 30s-120s;
In pH in 2.0 acid solution, the immersion treatment time is 3min-10min.
The acid is one kind in hydrochloric acid, sulfuric acid and phosphoric acid.
Comprise the following steps that:
1. it is 2.0 sulfuric acid or phosphoric acid solution to configure hydrochloric acid solution (pH is 1.0,2.0,3.0,4.0) and pH.
2. the good Porphyra yezoensis of growth conditions and Enteromorpha frond are thrown in different acid solutions, Dual-PAM is used
Determine photosynthetic parameters.
3. after acid treatment for a period of time, frond being taken out to be placed in nature seawater and recovered, photosynthetic parameters are determined.
Enteromorpha and Porphyra yezoensis are respectively placed in the hydrochloric acid solution that pH is 1.0,2.0,3.0 and 4.0 and handled by the present invention.
In the hydrochloric acid solution that pH is 1.0, Enteromorpha F after 30sv/FmValue be to be reduced to 0, and can not recover;After Porphyra yezoensis processing 2min
Recover 1 day, its photosynthetic activity can recover.In the hydrochloric acid solution that pH is 2.0, Enteromorpha F after 3minv/FmValue be reduced to 0, it is impossible to it is extensive
It is multiple;Even if recovering again after Porphyra yezoensis acid treatment 10min, its photosynthetic activity can also recover.In the hydrochloric acid solution that pH is 3.0,
F after Enteromorpha acid treatment 20minv/FmValue be reduced to 0, and can not recover;Recovered again after Porphyra yezoensis processing 20min, it is photosynthetic
Activity can be recovered.In the hydrochloric acid solution that pH is 4.0, Enteromorpha F after 120min acid treatmentv/FmValue remain unchanged it is very big,
The F of Porphyra yezoensisv/FmValue be remarkably decreased on the contrary, frond recover after can all recover photosynthetic activity.At the acid that pH is 3.0 and 4.0
The reason time is longer and effect is not good, is not suitable for removing the removing of miscellaneous algae Enteromorpha on cultivation lace curtaining.
Enteromorpha and Porphyra yezoensis are respectively placed in the sulfuric acid and phosphoric acid solution that pH is 1.0 and 2.0, its effect and phase by the present invention
It is similar with the HCl treatment result of pH value.After pH is 1.0 acid treatment 30s, Enteromorpha Fv/FmValue be to be reduced to 0, and can not
Recover;Recover after Porphyra yezoensis processing 2min, its photosynthetic activity can recover.In the acid solution that pH is 2.0, Enteromorpha after 3min
Fv/Fm0 is reduced to, is recovered 1 day in nature seawater, its photosynthetic activity is irrecoverable, it is still irrecoverable even if recovering 3 days;It is purple
After dish acid treatment 3min, Fv/FmValue be down to about 0.35 (value of normal frond is about 0.60), after recovering 1 day, its photosynthetic activity
Recover to normal level, even if acid treatment 5min (Fv/FmValue be about that 0.30), frond photosynthetic activity, which remains unchanged, to be recovered.
From above-mentioned, through certain density acid treatment for a period of time, Porphyra yezoensis can be made to cultivate the miscellaneous algae waterside on lace curtaining
Tongue is dead, the growth without influenceing Porphyra yezoensis frond.The acid treatment time is too short, does not reach the purpose for removing Enteromorpha;Acid treatment
Overlong time, can also influence the growth of Porphyra yezoensis.The pH of acid solution is too high, even if increase processing time, does not reach removing yet
The purpose of miscellaneous algae Enteromorpha, is not suitable for production application, and then find using specific processing mode, and for the specific of which
Optimal conditions cause high treating effect, are adapted to industrial applications.
The invention has the advantages that:
The present invention is using Porphyra yezoensis cultivation lace curtaining of the acid treatment with miscellaneous algae Enteromorpha, removing Enteromorpha (making frond dead)
The growth of Porphyra yezoensis is not influenceed, its is simple to operate, technique is simple, and processing time is short, method effectively, can improve Porphyra yezoensis
Yield and quality, improve the economic benefit of seaweed industry;Specially:
, only need to be by the Porphyra yezoensis cultivation lace curtaining leaching with miscellaneous algae Enteromorpha in practical operation of the present invention 1. operating process is simple
Bubble a period of time in acid solution.
2. wide, either hydrochloric acid, sulfuric acid, or phosphoric acid in process of the present invention are selected, in the acid that pH is 1.0 or 2.0
A period of time is handled in solution, you can make Enteromorpha dead without influenceing the growth of Porphyra yezoensis;And the acid solution used can
Recycling, the acid solution pH for treating cultivation lace curtaining changes and little, can reuse.
3. the time of saving and cost, the cultivation lace curtaining processing 30s-2min in pH is 1.0 acid solution, are 2.0 in pH
Lace curtaining processing 3-10min is cultivated in acid solution, you can make Enteromorpha dead without influenceing the growth of Porphyra yezoensis.
Brief description of the drawings
Fig. 1 is that the photosynthetic activity after Enteromorpha provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 1.0 changes, R
(recovery) the recovery figure in nature seawater is represented after frond acid treatment for a period of time.
Fig. 2 is that the photosynthetic activity after Porphyra yezoensis provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 1.0 changes
Figure.
Fig. 3 is the photosynthetic activity variation diagram after Enteromorpha provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 2.0.
Fig. 4 is that the photosynthetic activity after Porphyra yezoensis provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 2.0 changes
Figure.
Fig. 5 is the photosynthetic activity variation diagram after Enteromorpha provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 3.0.
Fig. 6 is that the photosynthetic activity after Porphyra yezoensis provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 3.0 changes
Figure.
Fig. 7 is the photosynthetic activity variation diagram after Enteromorpha provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 4.0.
Fig. 8 is that the photosynthetic activity after Porphyra yezoensis provided in an embodiment of the present invention is handled through the hydrochloric acid solutions of pH 4.0 changes
Figure.
Fig. 9 is photosynthetic activity variation diagram of the Enteromorpha provided in an embodiment of the present invention after the sulfuric acid solutions of pH 1.0.
Figure 10 is the photosynthetic activity variation diagram after Enteromorpha provided in an embodiment of the present invention is handled through the phosphoric acid solutions of pH 1.0.
Figure 11 is photosynthetic activity change of the Porphyra yezoensis provided in an embodiment of the present invention after the sulfuric acid solutions of pH 1.0
Figure.
Figure 12 is that the photosynthetic activity after Porphyra yezoensis provided in an embodiment of the present invention is handled through the phosphoric acid solutions of pH 1.0 changes
Figure.
Figure 13 is photosynthetic activity variation diagram of the Enteromorpha provided in an embodiment of the present invention after the sulfuric acid solutions of pH 2.0.
Figure 14 is the photosynthetic activity variation diagram after Enteromorpha provided in an embodiment of the present invention is handled through the phosphoric acid solutions of pH 2.0.
Figure 15 is photosynthetic activity change of the Porphyra yezoensis provided in an embodiment of the present invention after the sulfuric acid solutions of pH 2.0
Figure.
Figure 16 is that the photosynthetic activity after Porphyra yezoensis provided in an embodiment of the present invention is handled through the phosphoric acid solutions of pH 2.0 changes
Figure.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after present disclosure has been read, those skilled in the art can
To be made various changes or modifications to the present invention, these equivalent form of values equally fall within limited range of the present invention.
Embodiment 1, hydrochloric acid solution processing Enteromorpha and Porphyra yezoensis that pH is 1.0,2.0,3.0 and 4.0
It is 1.0,2.0,3.0 and 4.0 with pH meter regulation pH 1. adding concentrated hydrochloric acid in nature seawater.
2. the good Enteromorpha of growth conditions and Porphyra yezoensis are placed in the hydrochloric acid that pH is 1.0, determined using Dual-PAM
Photosynthetic parameters.About 30s, the F of Enteromorpha frondv/Fm0 is reduced to, frond is taken out and is placed on progress recovery 1 day in nature seawater,
Its photosynthetic activity can not recover.After Porphyra yezoensis processing 120s, Fv/Fm0.40 or so is down to, is now recovered 1 day, the photosynthetic work of frond
Property can be recovered;Handle 210s, Fv/Fm0.20 or so is down to, after now recovering 1 day, frond can not recover to normal level (ginseng
See Fig. 1 and 2).
From above-mentioned Fig. 1 and 2, pH 1.0 hydrochloric acid solution processing frond 30s to 120s, Enteromorpha can be made dead, together
When do not influence the growth of Porphyra yezoensis.
3. Enteromorpha and Porphyra yezoensis are placed in the hydrochloric acid that pH is 2.0, photosynthetic parameters are determined using Dual-PAM.About
3min, the F of Enteromorpha frondv/Fm0 is reduced to, frond is taken out and is placed on progress recovery 1 day and 3 days, its photosynthetic work in nature seawater
Property can not be recovered.Porphyra yezoensis processing 3min (Fv/FmBe down to 0.35), processing 5min (Fv/FmBe down to 0.30), processing 10min
(Fv/FmIt is down to 0.20), recovers 1 day, frond photosynthetic activity can recover;Handle 15min, Fv/FmLess than 0.10 is down to, this
After Shi Huifu 1 day and 3 days, frond can not recover to normal level (referring to Fig. 3 and 4).
Frond 3min-10min is handled from above-mentioned Fig. 3 and 4, pH 2.0 hydrochloric acid solution, Enteromorpha can be made dead, together
When do not influence the growth of Porphyra yezoensis.
4. Enteromorpha and Porphyra yezoensis are placed in the hydrochloric acid that pH is 3.0, photosynthetic parameters are determined using Dual-PAM.About
20min, the F of Enteromorpha frondv/FmValue be less than 0.1, be placed in nature seawater carry out recover 1 day, its photosynthetic activity can not
Recover to normal level.Porphyra yezoensis processing 20min (Fv/FmIt is down to 0.25), recovers 1 day, frond photosynthetic activity can recover;
Handle 30min, Fv/FmLess than 0.10 is down to, after now recovering 1 day and 3 days, frond can not recover to normal level (referring to Fig. 5
With 6).
Frond 20min is handled from above-mentioned Fig. 5 and 6, pH 3.0 hydrochloric acid solution, Enteromorpha can be caused dead, and not shadow
Ring Porphyra yezoensis growth.But it is due to that processing time is longer, cost increase is not suitable for the removing of miscellaneous algae Enteromorpha.
5. Enteromorpha and Porphyra yezoensis are placed in the hydrochloric acid that pH is 4.0, photosynthetic parameters are determined using Dual-PAM.About
120min, the F of Enteromorpha frondv/FmValue is compared with the control without significant difference, and the value conspicuousnesses of Y II decline, and are placed on nature
Recover 1 day in seawater, Y II recovers to normal level.Porphyra yezoensis processing 120min (Fv/FmIt is down to less than 0.20), Y II declines
To less than 0.10, recover 1 day, frond photosynthetic activity can recover (referring to Fig. 7 and 8).
It is not notable to the treatment effect of Enteromorpha from above-mentioned Fig. 7 and 8, pH 4.0 hydrochloric acid solution, it is not suitable for miscellaneous algae
The removing of Enteromorpha.
Embodiment 2, pH handles Enteromorpha and Porphyra yezoensis for 1.0 sulfuric acid solution and phosphoric acid solution
It is 1.0 with pH meter regulation pH 1. adding the concentrated sulfuric acid and concentrated phosphoric acid respectively in nature seawater.
2. Enteromorpha is respectively placed in the sulfuric acid solution and phosphoric acid solution that pH is 1.0, photosynthetic work is determined using Dual-PAM
With parameter, photosynthetic parameters F is treatedv/Fm(about 30s) takes out when being reduced to 0, is placed in nature seawater and recovers, its photochemical vitality can not
Recover (referring to Fig. 9 and 10).
From above-mentioned Fig. 9 and 10, Enteromorpha i.e. death after pH is 1.0 acid (sulfuric acid or phosphoric acid) solution processing 30s.
3. Porphyra yezoensis is respectively placed in the sulfuric acid solution and phosphoric acid solution that pH is 1.0, light is determined using Dual-PAM
Taken out after closing operational factors, processing 120s and 210s, be placed in nature seawater and recover 1 day.Handle after 120s, its photosynthetic work
Property can be recovered;Handle 210s, its photosynthetic activity can not return to normal level (referring to Figure 11 and 12).
From above-mentioned Figure 11 and 12, Porphyra yezoensis after pH 1.0 acid (sulfuric acid or phosphoric acid) solution processing 120s again
Recovered, its photosynthetic activity can recover.
Embodiment 3, pH handles Enteromorpha and Porphyra yezoensis for 2.0 sulfuric acid and phosphoric acid solution
It is 2.0 with pH meter regulation pH 1. adding the concentrated sulfuric acid and concentrated phosphoric acid respectively in nature seawater.
2. Enteromorpha is respectively placed in the sulfuric acid solution and phosphoric acid solution that pH is 2.0, photosynthetic work is determined using Dual-PAM
With parameter, photosynthetic parameters F is treatedv/Fm(about 3min) takes out when being reduced to 0, is placed in nature seawater and recovers 1 day and 3 days.Recovery is handled
Photosynthetic parameters are determined using Dual-PAM afterwards, it is found that Enteromorpha recovers 3 days in nature seawater, its photochemical vitality
Still irrecoverable (referring to Figure 13 and 14).
From above-mentioned Figure 13 and 14, Enteromorpha is i.e. dead after pH is 2.0 acid (sulfuric acid or phosphoric acid) solution processing 3min
Die.
3. Porphyra yezoensis is respectively placed in the sulfuric acid solution and phosphoric acid solution that pH is 2.0, light is determined using Dual-PAM
Close operational factors, processing 3min (Fv/FmAbout 0.35) and 5min (Fv/FmAbout 0.30) after take out, be placed in extensive in nature seawater
It is multiple 1 day.No matter Porphyra yezoensis is handled in 3min or 5min in acid solution, can be recovered after recovering 1 day to normal level (ginseng
See Figure 15 and 16).
From above-mentioned Figure 15 and 16, Porphyra yezoensis after pH 2.0 acid (sulfuric acid or phosphoric acid) solution processing 5min again
Recovered, its photosynthetic activity can recover.
The present invention removes Enteromorpha without influenceing streak purple using Porphyra yezoensis cultivation lace curtaining of the acid treatment with miscellaneous algae Enteromorpha
The growth of dish.Easy to operate, technique is simple, and processing time is short, and cost is low, improves the yield and quality of Porphyra yezoensis.
It is above the description to the embodiment of the present invention, by the foregoing description of the disclosed embodiments, makes this area special
Industry technical staff can realize or using the present invention.A variety of modifications to these embodiments come to those skilled in the art
Say and will be apparent, generic principles defined herein can not depart from the situation of the spirit or scope of the present invention
Under, realize in other embodiments.Therefore, the present invention is not intended to be limited to the embodiments shown herein, but to accord with
Close the most wide scope consistent with features of novelty with principles disclosed herein.
Claims (5)
1. a kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining, it is characterised in that:There is the Porphyra yezoensis of miscellaneous algae Enteromorpha by growing nonparasitically upon another plant
Lace curtaining, which is placed in acid solution, to be soaked, and is placed in nature seawater and is recovered again afterwards, Enteromorpha is removed, and the streak on lace curtaining
Seaweed is grown.
2. the method according to claim 1 for removing miscellaneous algae Enteromorpha on cultivation lace curtaining, it is characterised in that:The acid solution
PH is 1.0-2.0;Soak time is 30s-10min.
3. the method according to claim 2 for removing miscellaneous algae Enteromorpha on cultivation lace curtaining, it is characterised in that:The acid solution
PH is 1.0 or 2.0.
4. the method for miscellaneous algae Enteromorpha on the removing cultivation lace curtaining according to Claims 2 or 3, it is characterised in that:The immersion
In pH in 1.0 acid solution, the immersion treatment time is 30s-120s;
In pH in 2.0 acid solution, the immersion treatment time is 3min-10min.
5. the method according to claim 1 for removing miscellaneous algae Enteromorpha on cultivation lace curtaining, it is characterised in that:The acid is salt
One kind in acid, sulfuric acid and phosphoric acid.
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CN108751272A (en) * | 2018-05-18 | 2018-11-06 | 中国科学院海洋研究所 | A kind of method of acid processing Enteromorpha under low-salt conditions |
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Application publication date: 20171020 |