CN106717248A - A kind of method for extending the eelgrass Seed storage time - Google Patents
A kind of method for extending the eelgrass Seed storage time Download PDFInfo
- Publication number
- CN106717248A CN106717248A CN201611028977.XA CN201611028977A CN106717248A CN 106717248 A CN106717248 A CN 106717248A CN 201611028977 A CN201611028977 A CN 201611028977A CN 106717248 A CN106717248 A CN 106717248A
- Authority
- CN
- China
- Prior art keywords
- seed
- eelgrass
- water
- seed storage
- storage time
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001123263 Zostera Species 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 26
- 108010016634 Seed Storage Proteins Proteins 0.000 title claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000013535 sea water Substances 0.000 claims abstract description 25
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 3
- 238000004321 preservation Methods 0.000 claims description 17
- 238000001914 filtration Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 9
- 238000011084 recovery Methods 0.000 abstract description 7
- 238000010899 nucleation Methods 0.000 abstract description 2
- 240000007058 Halophila ovalis Species 0.000 abstract 1
- 241000544058 Halophila Species 0.000 description 24
- 230000000694 effects Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 5
- 230000007774 longterm Effects 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 244000025254 Cannabis sativa Species 0.000 description 3
- 240000002044 Rhizophora apiculata Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 238000005057 refrigeration Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 244000132059 Carica parviflora Species 0.000 description 2
- 235000014653 Carica parviflora Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000251511 Holothuroidea Species 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 241000238413 Octopus Species 0.000 description 1
- 208000028804 PERCHING syndrome Diseases 0.000 description 1
- 241000543657 Triglochin Species 0.000 description 1
- 241000196252 Ulva Species 0.000 description 1
- 241001148683 Zostera marina Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000003287 bathing Methods 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 125000000058 cyclopentadienyl group Chemical group C1(=CC=CC1)* 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- ZSWFCLXCOIISFI-UHFFFAOYSA-N endo-cyclopentadiene Natural products C1C=CC=C1 ZSWFCLXCOIISFI-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000007958 sleep Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The present invention relates to the store method of seed, specifically a kind of method for extending the eelgrass Seed storage time.Sterilized after common natural sea-water is filtered；The eelgrass seed that will be rinsed well is directly placed into the sealing container equipped with above-mentioned sterilizing seawater；Make to be maintained at 0.2-0.2 DEG C immersed with the ocean temperature of eelgrass seed；And during Seed storage, refresh the water periodically.Using the present invention retention rate during eelgrass Seed storage can improve, also the holding time of seed is extended, seed vigor higher and quality can be kept simultaneously, and improve eelgrass seedling builds up rate, with reference to the eelgrass Seed storage method that the present invention is simple and effective, high-quality eelgrass seed and healthy seedling can be provided for artificial seeding, be conducive to the recovery or reconstruction of eelgrass sea grass bed, for the good marine eco-environment of fast quick-recovery provides infrastructural support；Meanwhile, the present invention is preserved with the ordinary sea water of sterilizing, thus convenient, simple, practical, cost-effective.
Description
Technical field
The present invention relates to the store method of seed, specifically a kind of method for extending the eelgrass Seed storage time.
Background technology
Sea grass bed (Seagrass meadows) ecosystem and mangrove, coral reef ecologic system are considered as together near
The typical marine ecosystems of Hai San great.And international research result shows, sea grass bed Ecosystem Service Value is apparently higher than mangrove
With the Ecosystem Service Value of coral reef." sea grass " described here means unique angiosperm being submerged under shallow sea water, differentiation
Go out the organs such as root, stem, leaf, flower and seed, it is completely different with " marine alga " (such as sea-tangle, the Enteromorpha) as rudimentary plant.
Sea grass bed in great numbers, such as the forest of land, is the important symbol of healthy marine environment, is precious " seabed grass
It is former " or " submarine forest ".Sea grass bed is high productivity area, can provide good habitat for marine organisms such as fish shrimp crab shellfishes, educate
Seedling and protection ground, are also beneficial to perching for seabird；Simultaneously for many biologies provide important food source.Sea grass bed is also ground
Maximally effective carbon is captured and seals one of system up for safekeeping on ball, and the UN organizations think:The most urgent action of reply climate change is dimension
Hold and recover " blue carbon " to converge, sea grass is given priority to together with mangrove, sabkha.
Northern China temperate zone marine site, especially Along The Coast of Shandong Province, are that sea grass is distributed important region.Eelgrass (Zostera
Marina L.) it is a kind of coastal distribution of the northern China most most sea grass of wide, quantity, and only it is distributed in northern China temperate zone sea
Domain, such as Shandong, Hebei and Liaoning coastal waters.Eelgrass sea grass bed is sea cucumber, shellfish (including octopus), Shrimp waste and fish etc.
The good place that marine economy biology is perched, breeds and hidden oneself.Eelgrass is herbaceos perennial, and main heavy to be born in physical features gentle
Argillo arenaceous shallow water seabed, the shallow water area from intertidal zone to subtidal zone is all distributed, and maximum water depth is up to 30 meters, and its leaf is elongated
In banding, Triglochin martimum is commonly called as, leaf is long up to more than 3 meters.
In northern China temperate zone marine site, such as Along The Coast of Shandong Province, eelgrass sea grass bed was once very in great numbers, seriously moved back now
Change, sea grass is sparse in block distribution, and piece is not linked to be mostly.In northern China, coastal fisherman often makees construction house with eelgrass sea grass
The material on top.Seaweed house is the exclusive significant folk custom symbol in northern Shandong, is also considered as the most representational ecological people in the world
One of occupy, it is mainly distributed on the coastal areas such as Weihai, Yantai, Qingdao.This illustrates that conventional sea grass is how numerous from another side
Cyclopentadienyl, the now is but stayed very few.According to estimates, Along The Coast of Shandong Province eelgrass sea grass bed deteriorates more than 80%.
For a long time, important function of the sea grass in ocean does not obtain enough attention, and sea grass bed area is in global model
Interior decline is enclosed, many marine sites are on the brink of to become extinct, and injure the existence of other marine organisms, and bio-diversity is also decreased, edge
The bank ecosystem also becomes more fragile.
In recent years, people start to recognize important function of the sea grass in marine ecosystems, start to pay attention to give birth to sea grass
The protection of state system and the ecological recovery of the sea grass ecosystem to degenerating.Sea grass kind is carried out using the eelgrass seed for artificially collecting
It is one of the important technical for carrying out sea grass bed reconstruction and recovery that word bank is set up.It is currently, there are the long-term preservation of eelgrass seed
Problem, lacks simple and effective eelgrass seed long-term preservation method.At present, eelgrass Seed storage method has patent CN
High salt conditions (45psu~70psu) are preserved at published preservation condition, i.e., 4 DEG C in 101816229A.In practical application, should
The defect of store method is but easily seed is entered deep sleep state, next spring after planting dormancy under high salt preservation condition
In seed be difficult the short time " revival " and come, typically at least need could start rudiment within one month, the eel for causing field to sow
Grass seed has very big loss because marine organisms ingest and are easily taken away by stormy waves, reduces field seed germination rate；Although
The seed that patent CN 101816229A statement high salts are preserved germination rate under low-salt conditions is higher, but the eelgrass that the method is preserved
Seed seedling establishment rate is relatively low, it is difficult to complete building up for eelgrass seedling under field condition.Therefore, high salt preservation condition not only slows down
The after planting rudiment of seed, and seriously suppression and reduction eelgrass seedling build up, so as to be extremely difficult to the recovery effects of anticipation.Separately
Outward, 4 DEG C of preservation seeds, are common knowledges, and various seeds can be preserved in this temperature.But for sea-plant
For eelgrass seed, 4 DEG C of preservations, shortcoming will start to sprout it is obvious that seed is in the preservation of 4 DEG C of ordinary sea water after 1-3 month,
The purpose that seed longer-term preserves (the spring sowing for being at least saved in next year) is not reached.In addition, with common sea under the conditions of 4 DEG C
Water preserves seed, and it is still relatively frequent to change water, and seed because corrupt, loss late is still higher.
In a word, effective eelgrass seed long-term preservation method is currently lacked.Variation of Salinity Condition high, beyond seed from
Category is so tolerated, the infringement to seed is obvious.Seed is preserved under the conditions of 4 DEG C of common refrigeration case, it is clear that for eelgrass, preserve effect
It is really very poor.
The content of the invention
It is an object of the invention to provide a kind of method for extending the eelgrass Seed storage time.
To achieve the above object, the present invention use technical scheme for：
A kind of method for extending the eelgrass Seed storage time, is sterilized after common natural sea-water is filtered；To rinse
Clean eelgrass seed is directly placed into the sealing container equipped with above-mentioned sterilizing seawater；Make the ocean temperature immersed with eelgrass seed
It is maintained at-0.2-0.2 DEG C；And during Seed storage, refresh the water periodically.
32~the 35psu of its salinity range of ordinary sea water for preserving seed is advisable.
The ocean temperature of the immersion eelgrass seed is 0 DEG C.
It is described to refresh the water periodically as every month changes the sterilizing seawater after once filtering.
The seed of corruption is refreshed the water periodically and cleared up during the preservation.
During the corrupt seed of the cleaning, carried out in 0 DEG C of water-bath, it is to avoid water temperature it is significantly raised, stimulate seed to sprout
Bud.
The beneficial effects of the invention are as follows：
Using the method for present invention extension eelgrass Seed storage time, preserved using under the conditions of 0 DEG C of nature seawater, can promoted
Enter seed and keep synchronous resting state, without rudiment, so that the retention rate during improving Seed storage, and eelgrass seed can be extended
Holding time；Simultaneously eelgrass seed vigor higher and quality can be kept, and improve eelgrass seedling build up rate.Profit
High-quality eelgrass seed and healthy seedling can be provided for artificial seeding with the inventive method, be conducive to the recovery of eelgrass sea grass bed
With structure, the good marine eco-environment of fast quick-recovery.
In the preservation of seed, common knowledge is that 4 DEG C of conventional common refrigeration casees are preserved.But for eelgrass seed, 4 DEG C of preservations
Shortcoming, seed will start to sprout after 1-3 month, not reach seed longer-term and preserve (at least in the preservation of 4 DEG C of ordinary sea water
Be saved in next year spring sowing) purpose.In addition, preserving seed with ordinary sea water under the conditions of 4 DEG C, change water and still compare frequency
It is numerous, and seed because corrupt, loss late is still higher.The present invention (preferably 0 DEG C) under the conditions of -0.2-0.2 DEG C preserves eelgrass kind
Son, its Heterosis is in following several respects：First, it is ensured that under conditions of seed greater activity, eelgrass seed is greatly extended
Holding time, reached the purpose of long-term preservation；Secondly, under -0.2-0.2 DEG C of (preferably 0 DEG C) preservation condition, eelgrass seed is rotten
Rate is lost well below the Decayed rate under the conditions of 4 DEG C, therefore substantially increases the seed retention rate during eelgrass Seed storage；Most
Afterwards, due under the conditions of -0.2-0.2 DEG C (preferably 0 DEG C) Decayed rate well below 4 DEG C under the conditions of, therefore reduce eelgrass seed guarantor
Water frequency is changed during depositing, human cost is significantly reduced, the disturbance to preserving seed is reduced, it is to avoid germination.Separately
Outward, during Seed storage, it is -0.2-0.2 DEG C (preferably 0 DEG C) to change the new water water temperature changed during water to the present invention, and without room
Warm seawater, it is therefore an objective to avoid the rising of water temperature and stimulate germination；Equally in the corrupt seed of cleaning, in 0 DEG C of water-bath
In carry out, stimulate seed germination also for the significantly raised of water temperature is avoided.
In a word, effective eelgrass seed long-term preservation method is currently lacked.Variation of Salinity Condition high, beyond seed from
Category is so tolerated, the infringement to seed is obvious.Seed is preserved under the conditions of 4 DEG C of common refrigeration case, it is clear that for eelgrass, preserve effect
It is really very poor.
Brief description of the drawings
Fig. 1 is a kind of method and step flow chart for extending the eelgrass Seed storage time of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawings to further detailed description of the present invention.
Not only the retention rate of seed is high during Seed storage for the inventive method, while the good of eelgrass seed can be kept
Quality and vigor, and substantially prolongs the holding time of eelgrass seed, and improve eelgrass seedling build up rate.
As shown in figure 1, its step is：
By ordinary sea water (32-35psu) through the glass fibre membrane filtration of a diameter of 47 millimeters of filter opening, 5000 milliliters of filterings are taken
Seawater afterwards is put into full-automatic high-pressure autoclave the 5h that sterilizes.
1000 eelgrass seeds are put into sealing preserve in the wide-mouth bottle for filling sterilizing seawater, are preserved under the conditions of being placed in 0 DEG C.
Embodiment
5000 milliliters of ordinary sea waters (34psu) are carried out into filtration sterilization.1000 eelgrass seeds are randomly selected, is put into and is filled
During salinity is for the wide-mouth bottle of 34psu sterilizing seawater, it is placed in after good seal under the conditions of 0 DEG C of temperature.An inferior salinity is carried out weekly to go out
Bacterium seawater is changed, and checks seed situation within every 2 months, and the seed to sprouting is counted, and randomly selects the eelgrass kind of non-rudiment
Son carries out seed activity inspection (rudiment experiment), and the duration is 13 months.Eel is preserved under the conditions of salt angle value 34psu, 0 DEG C of temperature
Grass seed retention rate and seedling establishment rate record are as shown in table 1.
Table 1
| Time | Retention rate (%) | Seedling establishment rate (%) |
| In August, 2015 | 100 | 93 |
| In September, 2015 | 92 | 90 |
| In November, 2015 | 86 | 91 |
| In January, 2016 | 82 | 63 |
| In March, 2016 | 80 | 56 |
| In May, 2016 | 79 | 50 |
| In July, 2016 | 65 | 65 |
| In September, 2016 | 59 | 64 |
Its retention rate is up to 80% after eelgrass seed is preserved 7 months under the conditions of embodiment；After 11 months, most of eelgrass
Seed (65%) still retains and rots, and does not occur in rotten seed about 65% and remains in that activity and can complete children
Seedling builds up；After the eelgrass seed for preserving of the invention 13 months, its retention rate is 59%, the seedling of the eelgrass seed for retaining
The rate of building up is up to 64%.Therefore, it is stored in 0 DEG C of filtration sterilization ordinary sea water, the holding time of eelgrass seed is obviously prolonged, can
At least 13 months are extended to by some months of original effectively holding time, the eelgrass seed for preserving 13 months still has kind higher
Sub- retention rate and seedling establishment rate.Application of the invention will keep the premise of eelgrass seed greater activity and seedling establishment rate
Under, the Seed storage time is greatly prolonged, so as to realize the foundation of eelgrass seed bank, and reduce human cost.The present invention is related to
And to the specific data of seedling establishment rate be by test obtain, that is, 50, eelgrass seed (3 weights do not sprouted after depositing of going bail for
It is multiple) it is immersed in the container for filling nature seawater (34psu), in 15 DEG C of temperature, photoperiod 12L：Quiescent culture 2 under the conditions of 12D
Individual month, seedling establishment number is recorded, and seedling establishment rate is calculated.
The model gi54dw's that the high-pressure sterilizing pot that the present embodiment is used is produced for Zealway (Xiamen) Instrument Inc.
Full-automatic high-pressure autoclave, the source water of the present embodiment sterilizing seawater is the second bathing beach of Qingdao City seawater.
Pure water used by the present embodiment is the pure water of water purification machine process for producing, and the water purifior used by the present embodiment is ultrapure for Chengdu
The water purifior of the model UPT-H-10 of Science and Technology Ltd.'s production, the source water of the present embodiment water purifior is municipal tap water.
0 DEG C keeps steady temperature, product type using low temperature incubator in the present embodiment：KRC-150CA, manufacturer：
Shanghai Qi Xin scientific instrument Co., Ltd；Other temperature and lighting requirements keep steady temperature and light using intelligent illumination box
According to product type：MGC-250, manufacturer：The permanent Science and Technology Ltd. in Shanghai one.
Claims (6)
1. it is a kind of extend the eelgrass Seed storage time method, it is characterised in that will common natural sea-water filter after be gone out
Bacterium；The eelgrass seed that will be rinsed well is directly placed into the sealing container equipped with above-mentioned sterilizing seawater；Make immersed with eelgrass seed
Ocean temperature be maintained at-0.2-0.2 DEG C；And during Seed storage, refresh the water periodically.
2. the method for extending the eelgrass Seed storage time according to claim 1, it is characterised in that：It is described to preserve the general of seed
32~35psu of its salinity range of maritime water is advisable.
3. the method for extending the eelgrass Seed storage time according to claim 1, it is characterised in that：The immersion eelgrass seed
Ocean temperature be 0 DEG C.
4. the method for extending the eelgrass Seed storage time according to claim 1, it is characterised in that：Described refreshing the water periodically is every
The sterilizing seawater changed after once filtering for individual month.
5. the method for extending the eelgrass Seed storage time according to claim 1, it is characterised in that：It is fixed during the preservation
Phase changes water and clears up the seed of corruption.
6. the method for extending the eelgrass Seed storage time according to claim 5, it is characterised in that：The corrupt kind of the cleaning
The period of the day from 11 p.m. to 1 a.m, is carried out in 0 DEG C of water-bath.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611028977.XA CN106717248A (en) | 2016-11-22 | 2016-11-22 | A kind of method for extending the eelgrass Seed storage time |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611028977.XA CN106717248A (en) | 2016-11-22 | 2016-11-22 | A kind of method for extending the eelgrass Seed storage time |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106717248A true CN106717248A (en) | 2017-05-31 |
Family
ID=58970507
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611028977.XA Pending CN106717248A (en) | 2016-11-22 | 2016-11-22 | A kind of method for extending the eelgrass Seed storage time |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106717248A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593687A (en) * | 2017-09-12 | 2018-01-19 | 中国科学院海洋研究所 | A kind of method of river creeping weed Seed storage |
| CN110973122A (en) * | 2019-12-18 | 2020-04-10 | 中国科学院海洋研究所 | Antibacterial preservation method for zostera marina seeds |
| CN116171850A (en) * | 2023-02-10 | 2023-05-30 | 中国水产科学研究院黄海水产研究所 | System and method for large-scale long-term preservation of seaweed seeds based on upward flow |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232345A (en) * | 2010-04-30 | 2011-11-09 | 中国科学院海洋研究所 | Method for budding eelgrass seeds, cultivating seedlings and recovering sea grass bed |
-
2016
- 2016-11-22 CN CN201611028977.XA patent/CN106717248A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232345A (en) * | 2010-04-30 | 2011-11-09 | 中国科学院海洋研究所 | Method for budding eelgrass seeds, cultivating seedlings and recovering sea grass bed |
Non-Patent Citations (3)
| Title |
|---|
| MAHIKO ABE ET AL.: ""Temperature characteristics in seed germination and growth of Zostera japonica Ascherson & Graebner from Ago Bay,Mie Prefecture, central Japan"", 《FISH SCI》 * |
| MAHIKO ABE ET AL: ""Temperature requirements for seed germination and seedling growth of Zostera marina from center Japan"", 《FISHERIES SCIENCE》 * |
| 岩濑荣一: "《盐及其化学》", 31 October 1960, 轻工业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593687A (en) * | 2017-09-12 | 2018-01-19 | 中国科学院海洋研究所 | A kind of method of river creeping weed Seed storage |
| CN107593687B (en) * | 2017-09-12 | 2020-12-04 | 中国科学院海洋研究所 | Method for preserving chuanman grass seeds |
| CN110973122A (en) * | 2019-12-18 | 2020-04-10 | 中国科学院海洋研究所 | Antibacterial preservation method for zostera marina seeds |
| CN116171850A (en) * | 2023-02-10 | 2023-05-30 | 中国水产科学研究院黄海水产研究所 | System and method for large-scale long-term preservation of seaweed seeds based on upward flow |
| CN116171850B (en) * | 2023-02-10 | 2023-09-19 | 中国水产科学研究院黄海水产研究所 | System and method for large-scale long-term preservation of seaweed seeds based on upward flow |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sahoo et al. | Mariculture of seaweeds | |
| CN102232345B (en) | Method for budding eelgrass seeds, cultivating seedlings and recovering sea grass bed | |
| Kuntz et al. | Effects of water nutrients on regeneration capacity of submerged aquatic plant fragments | |
| CN104285747B (en) | A kind of method of mangrove sea paint seedling breeding cultivation | |
| CN104430079A (en) | Vegetable, rice and flower planting and net cage intercropping combination device and method | |
| CN104351034B (en) | A kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology | |
| CN205455363U (en) | Multi -functional chlamys nobilis breeds cover cylinder mould | |
| CN106717248A (en) | A kind of method for extending the eelgrass Seed storage time | |
| CN1806491A (en) | Method for breeding Sonneratia apetala sprout | |
| CN108668880A (en) | A kind of semi-submersible type sea grass breeding apparatus and breeding method | |
| CN104145804B (en) | Eel grass seed proliferation device and method adaptable to rapid water flow conditions | |
| CN108990675B (en) | Method for cuttage breeding of tung tree | |
| CN103609422B (en) | Zostera marina seed multiplication method | |
| CN103518657A (en) | Intermediate cultivating method capable of improving survival rate of pinctada maxima spats | |
| CN103270947A (en) | Duvalia angustiloba tissue culturing method | |
| CN107593687B (en) | Method for preserving chuanman grass seeds | |
| CN105475270B (en) | A kind of store method of Zostera marina seedling | |
| CN102657125A (en) | Application of floating deep-water cages to stichopus japonicas cultivation and stichopus japonicas cultivation method using floating deep-water cages | |
| KR20180036450A (en) | Equipment for planting halophyte and method of planting halophyte | |
| CN106258968A (en) | The iris tissue culture method that a kind of browning rate is low | |
| CN113925058A (en) | Mixed acid treatment solution for removing sessile green algae on porphyra yezoensis cultivation raft and method for removing green algae by using mixed acid treatment solution | |
| CN107455042B (en) | Method for drying and preserving chuanman grass seeds | |
| CN203985414U (en) | The anxious flow condition grass wrack seeds propagation of a kind of adaptation device | |
| JP2021078438A (en) | Plane arrangement type bivalve rearing system | |
| CN103907466A (en) | Method for utilizing slack-winter-season mariculture pond to plant New Zealand spinach |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |
|
| RJ01 | Rejection of invention patent application after publication |