CN116171850B - System and method for large-scale long-term preservation of seaweed seeds based on upward flow - Google Patents
System and method for large-scale long-term preservation of seaweed seeds based on upward flow Download PDFInfo
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- CN116171850B CN116171850B CN202310108591.3A CN202310108591A CN116171850B CN 116171850 B CN116171850 B CN 116171850B CN 202310108591 A CN202310108591 A CN 202310108591A CN 116171850 B CN116171850 B CN 116171850B
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
- A01F25/14—Containers specially adapted for storing
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/24—Treatment of water, waste water, or sewage by flotation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
- C02F1/32—Treatment of water, waste water, or sewage by irradiation with ultraviolet light
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The invention provides a large-scale long-term seaweed seed preservation system based on upflow, which comprises a plurality of seed suspension devices, an air inlet pipeline, a water inlet pipeline and a water return pipeline, wherein the seed suspension devices comprise an organic glass barrel, an air disc support and a separation net, the air disc support is arranged at a certain height away from the barrel bottom of the organic glass barrel, the separation net is arranged in the organic glass barrel and is positioned above the air disc support, the air inlet pipeline is communicated with the lower part of the organic glass barrel, the water inlet pipeline is communicated with the bottom of the organic glass barrel through a water inlet, and the water return pipeline is communicated with the upper part of the organic glass barrel through a water return port. Based on the same inventive concept, the invention also provides a large-scale long-term preservation method of seaweed seeds based on upward flow. The sea weed seed large-scale long-term preservation system based on the upward flow provided by the invention has the advantages that the retention period of the sea weed seeds is more than 12 months; after 12 months, the seed germination rate was kept at 82.5-83.1% before preservation.
Description
Technical Field
The invention belongs to the field of seaweed seed preservation, and particularly relates to a large-scale long-term seaweed seed preservation system and method based on upward flow.
Background
Seaweed beds are one of the typical marine ecosystems and have extremely high primary productivity and ecological service value. Repair of seaweed beds is not sustained. In the current repair activity, grass bed repair by utilizing seeds is an important mode, does not influence the existing grass beds, and is beneficial to maintaining population gene diversity. However, the seeds can be effectively stored regardless of whether the seeds are directly sown or transplanted after artificial cultivation. At present, research reports on a preservation method of sinking seaweed seeds represented by temperate dominant species, namely eel grass, are available, namely the seeds are preserved at a low temperature of 0-4 ℃ under a sterile condition, so that germination of the seeds is inhibited, and vigor of the seeds is maintained. However, this approach is demanding and the seeds must be sterile and free of impurities.
However, in the large-scale seaweed bed repairing engineering, the operation of primarily sorting and removing the large-particle impurities from the seeds is very tedious and time-consuming, and the removal of the small-particle impurities is more difficult to realize, so that the sterile condition cannot be realized, and the seeds are stored on a large scale. The main reasons for the failure of seed preservation under non-sterile conditions are that the seeds are extremely easy to grow mould, the mass propagation of the mould causes dissolution of the content, so that the seeds lose vigor, and the low-oxygen microenvironment caused by the mould promotes the premature germination of other surrounding seeds, so that the failure of seed preservation is further caused.
Therefore, the problems in the prior art need to be solved, and how to design a seed large-scale preservation device and method suitable for the development of seaweed restoration industry is the technical problem to be solved by the invention.
Disclosure of Invention
The invention aims to solve the problems in the background technology and provides a large-scale long-term seaweed seed preservation system and method based on upward flow. According to the large-scale long-term seaweed seed preservation system and method based on the upflow, provided by the invention, the saturated oxygen system is constructed to inhibit germination of seeds, enable the seeds to suspend continuously, inhibit growth of mould, and additionally a circulating filtration and low-temperature system is used to realize large-scale and light-simplified preservation of the seeds, so that the requirement of seed preservation in repair engineering is met.
The technical aim of the invention is realized by the following technical scheme:
the utility model provides a sea weed seed scale long-term preservation system based on upflow, its characterized in that includes a plurality of seed suspending device, air inlet pipeline, water inlet pipeline and return line, seed suspending device includes organic glass bucket, gas dish support and separation net, the gas dish support sets up in the certain altitude department apart from the barrel head of organic glass bucket, the separation net sets up in the organic glass bucket and is located the top of gas dish support, the air inlet pipeline communicates in the below of organic glass bucket, the water inlet pipeline communicates in the bottom of organic glass bucket through the water inlet, the return line communicates in the top of organic glass bucket through the return water mouth.
The large-scale long-term seaweed seed preservation system based on the upward flow is characterized in that the air disc support is of an organic glass structure, the outer ring is annular, and the inner part of the air disc support is shaped like a Chinese character 'mi'; the nanometer micropore trachea winds many circles in the outer lane inside of gas dish support, forms the gas dish, the fixed setting of gas dish is on the gas dish support, gas dish intercommunication admission line.
The seaweed seed large-scale long-term preservation system based on the upward flow comprises a separation net support and a first stainless steel net, wherein the first stainless steel net is formed by pressing a double-layer organic glass ring, an annular gasket is arranged on the upper portion of the first stainless steel net and is pressed by a plastic ring and fastened by screws, and the annular gasket is tightly attached to the barrel wall of the organic glass barrel.
The seaweed seed large-scale long-term preservation system based on the upward flow, which is disclosed by the invention, further comprises a rope and/or a flexible barrel-shaped net bag, wherein the rope is used for lifting the seaweed seeds out of the organic glass barrel, the rope is fixedly connected to an organic glass ring of the separation net, and the organic glass ring is fixedly arranged on the inner wall of the organic glass barrel; the flexible barrel-shaped net is arranged in the organic glass barrel and turned out of the organic glass barrel, and the first stainless steel net and the blocking net are positioned in the flexible barrel-shaped net and fix the flexible barrel-shaped net on the inner wall of the organic glass barrel.
According to the large-scale long-term seaweed seed preservation system based on the upward flow, the bottom of the organic glass barrel is further provided with the water outlet for draining water in the organic glass barrel, and the water outlet is positioned at the opposite side of the water inlet.
According to the large-scale long-term seaweed seed preservation system based on the upward flow, the water overflow preventing port is further arranged above the water return port. Preferably, a cylindrical filter screen is arranged on the water return port.
According to the large-scale long-term seaweed seed preservation system based on the upward flow, the top of the organic glass barrel is provided with the blocking net.
The seaweed seed large-scale long-term preservation system based on the upward flow further comprises a first water storage barrel, a second water storage barrel, a protein separator and a microporous filter, wherein the first water storage barrel is connected with a water inlet pipeline, external seawater is coarsely filtered and stored in the first water storage barrel and then flows into the second water storage barrel, then part of water sequentially enters the organic glass barrel through the microporous filter, part of water enters the protein separator to remove protein, and the second water storage barrel is returned; seawater in the organic glass barrel sequentially enters the first water storage barrel, the second water storage barrel, the microporous filter and the protein separator through the filter cotton through the water return pipeline, and then enters the organic glass barrel again, and the first water storage barrel, the second water storage barrel, the microporous filter, the protein separator and the organic glass barrel are communicated to form a circulation loop.
Based on the same inventive concept, the invention also provides a large-scale long-term seaweed seed preservation method based on upward flow, which comprises the following steps:
s1, after the seaweed seeds are ripe and fall, collecting a separating net arranged in an organic glass barrel, after the seaweed seeds are placed, pressing an upper opening of the organic glass barrel by using a blocking net, opening a water inlet pipeline to store water, roughly filtering external seawater by using filter cotton and storing the seawater into a first water storage barrel, and then opening a protein separator, a microporous filter, an ultraviolet sterilizer and a water inlet pipeline until the water level of the first water storage barrel is 10cm below the upper edge of the first water storage barrel, closing external water inlet, and continuously and circularly operating a seaweed seed large-scale long-term storage system;
s2, simultaneously adjusting the temperature of a cooling chamber where a large-scale long-term seaweed seed preservation system is located to 0 ℃; and opening a valve of a water outlet of each organic glass barrel every 3-4 weeks, draining water in the system, and replacing new water.
According to the large-scale long-term seaweed seed preservation method based on the upward flow, the seed preservation quantity of each organic glass barrel is 10-100 ten thousand.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. the sea weed seed large-scale long-term preservation system based on the upward flow provided by the invention has the advantages that the retention period of the sea weed seeds is more than 12 months; after 12 months, the seed germination rate was kept at 82.5-83.1% before preservation.
2. The large-scale long-term preservation method for seaweed seeds based on upward flow provided by the invention has the advantages that complicated treatments such as disinfection and the like are not needed for the seeds, and the operation is simple.
Drawings
FIG. 1 is a schematic flow diagram of a large-scale long-term preservation system for seaweed seeds based on upflow provided by the invention;
FIG. 2 is a schematic diagram of the organic glass barrel according to the present invention;
FIG. 3 is a schematic diagram of the structure of the air disk support provided by the invention;
FIG. 4 is a schematic diagram of the structure of the air disk provided by the invention;
FIG. 5 is a graph showing the comparison of the numbers of water body cells after 1 day, after 2 days, after 5 days, and after 7 days, respectively, of the control group, example 1 (seed density of 20 thousand grains/barrel), and example 2 (seed density of 40 thousand grains/barrel) provided by the present invention.
Fig. 6 is a plot of germination ratio of seeds provided by the present invention for the control group, example 1 (seed density of 20 thousand grains/barrel) and example 2 (seed density of 40 thousand grains/barrel) after 15 days, after 2 months, after 6 months and after 12 months, respectively, relative to prior to storage.
In the figure, a 101-organic glass barrel, a 102-air disk bracket, a 103-separation net, a 104-air disk, a 105-water outlet, a 106-water inlet, a 107-water return, a 2-air inlet pipeline, a 3-water inlet pipeline, a 4-water return pipeline, a 5-air pump, a 6-water pump, a 7-first water storage barrel, an 8-protein separator, a 9-second water storage barrel, a 10-microporous filter and an 11-ultraviolet sterilizer.
Detailed Description
The following description of the embodiments of the present invention will clearly and fully describe the technical solutions of the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used in this specification includes any and all combinations of one or more of the associated listed items.
The equipment, devices, etc. used in the examples described below are commercially available unless otherwise specified. The ultraviolet sterilizer of the invention is purchased from model ZKH-ZW4003 of the sea water treatment Co., ltd, and the protein separator is purchased from model ZKH-DB20 of the sea water treatment Co., ltd. The seaweed seed large-scale long-term preservation system of the invention utilizes the air pump to supply air, and the air pump is a daily LP200 model oxygenation pump product. The inner diameter of the nanometer micropore trachea is 20mm, and the nanometer micropore trachea is commercially available.
The utility model provides a sea weed seed scale long-term preservation system based on upflow, includes a plurality of seed suspending device, air inlet pipeline, water inlet pipeline and return water pipeline, seed suspending device includes organic glass bucket, gas dish support and separation net, the gas dish support sets up in the certain altitude department of the barrel head apart from organic glass bucket, the separation net sets up in organic glass bucket and is located the top of gas dish support, the air inlet pipeline communicates in the below of organic glass bucket, the water inlet pipeline communicates in the bottom of organic glass bucket through the water inlet, the return water pipeline communicates in the top of organic glass bucket through the return water mouth.
The principle of the invention is as follows: the upward flow refers to water inflow through a bottom air disc and a water pipe, so that a water body rises, seeds are brought from the bottom of a container for storing seeds to the top of the container for storing seeds and kept in suspension continuously. In the present invention, sea weed seeds are preferably eel seeds. Eel seeds are easy to mildew, the vigor state is affected, and germination is easy to occur under high temperature and low oxygen conditions. In the invention, the air inlet pipeline comprises a main air inlet pipeline and a sub air inlet pipeline, air enters the sub air inlet pipes of each organic glass barrel through the main air inlet pipeline, enters the organic glass barrel, and is dispersed by the air disc formed by the nano microporous air pipes to form ascending air flow, the ascending air flow can prevent mildew generated by standing and stacking of seeds, and sufficient oxygen and low temperature are provided to prevent seed germination. In order to facilitate checking of the state and the ascending state of seeds in the preservation process, a main body preservation container of the system adopts an organic glass barrel made of transparent materials; in order to ensure that the seeds are in a good preservation state, the seeds in the system are in continuously circulating seawater. The method comprises the following steps: the external seawater enters a preserved organic glass barrel after filtration and sterilization, then water flows back to a main backwater main pipeline from the organic glass barrel, and dissolved substances such as protein and the like enter the barrel body after filtration, sterilization and removal in sequence, and the circulation is continued. In the invention, the air disc support is arranged at a certain height from the bottom of the organic glass barrel, as shown in fig. 2, the height of the organic glass barrel is set to be L, the height distance from the air disc support to the bottom of the organic glass barrel is set to be H, namely the height ratio between L and H is 7-12. For example, if the height L of the organic glass barrel is set to be 108cm, the air disc support is arranged at a position which is 10-15cm away from the barrel bottom of the organic glass barrel, more preferably, the air disc support is arranged at a position which is 10-12cm away from the barrel bottom of the organic glass barrel, most preferably, the air disc support is arranged at a position which is 10cm away from the barrel bottom of the organic glass barrel or 12cm away from the barrel bottom, because under the height, on one hand, seaweed seeds are kept at a distance from the barrel bottom of the organic glass barrel, keep to be suspended, and are not accumulated still on the barrel bottom, so that the problem that hypoxia and mildew are easily caused by seed accumulation is avoided, and on the other hand, more space above the organic glass barrel is used for suspending seaweed seeds.
Preferably, the air disk support is of an organic glass structure, the outer ring is annular, and the inner part of the air disk support is in a shape like a Chinese character 'mi'; the nanometer micropore trachea winds many circles in the outer lane inside of gas dish support, forms the gas dish, and as shown in fig. 3-4, nanometer micropore trachea can be for regular spiral multiturn, of course also can irregular spiral multiturn, the gas dish is fixed to be set up on the gas dish support, gas dish intercommunication admission line. In the invention, the shape and structure of the air disc bracket can better provide support for the nanometer micropore air pipe. The formed air disk can form large-area and uniform bubbles, carry seaweed seeds to float upwards, and is coiled for a plurality of circles so as to ensure that the air is better dispersed in the organic glass barrel.
Preferably, the separation net comprises a separation net support and a first stainless steel net, the first stainless steel net is formed by pressing a double-layer organic glass ring, an annular gasket is arranged on the upper portion of the first stainless steel net and is pressed by a plastic ring, the annular gasket is fastened by screws, and the annular gasket is tightly attached to the barrel wall of the organic glass barrel to play a role in sealing and pressing.
Preferably, the seed suspension device further comprises a rope and/or a flexible barrel-shaped net bag for lifting seaweed seeds out of the plexiglas barrel. The rope is fixedly connected to the organic glass ring of the separation net, the organic glass ring is fixedly arranged on the inner wall of the organic glass barrel, the flexible barrel-shaped net is a soft yarn net, for example, a chemical fiber net with soft texture can be selected, the flexible barrel-shaped net is arranged in the organic glass barrel and turned out of the organic glass barrel, the first stainless steel net and the blocking net are positioned in the flexible barrel-shaped net and are fixed on the inner wall of the organic glass barrel, and the flexible barrel-shaped net is used for conveniently collecting seaweed seeds after the storage is finished. After the seaweed seeds are stored, or when the stored seaweed seeds need to be taken out, the seeds are pulled out of the barrel under the combined action of the rope and the flexible barrel-shaped net bag.
Preferably, a water outlet for draining water in the organic glass barrel is further arranged at the bottom of the organic glass barrel, and the water outlet is positioned at the opposite side of the water inlet. The valve of the water outlet of each organic glass barrel is opened every 3-4 weeks, water in the system is drained, and new water is replaced, because substances such as soluble amino acid proteoglycan and the like are released in the seed preservation process, and the water is changed periodically in order to ensure that dissolved oxygen is not influenced, seed change is not caused.
Preferably, a cylindrical filter screen is arranged on the water return port; and an anti-overflow port is also arranged above the water return port. The cylindrical filter screen is formed by adding a stainless steel net after strip-shaped slotting of the organic glass barrel, and the strip-shaped slotting is inserted into a water return port of the organic glass barrel and used for blocking seeds and preventing the seeds from entering a water return pipeline along with water flow. The distance between the water return opening and the top of the organic glass barrel is 14-16 cm, and the distance between the water overflow preventing opening and the top of the organic glass barrel is 7-9 cm. Most preferably, the distance from the water return port to the top of the organic glass barrel is 15cm, and the distance from the water overflow preventing port to the top of the organic glass barrel is 8cm.
Preferably, a blocking net is arranged at the top of the organic glass barrel. The blocking net is located at a position 5cm away from the top of the organic glass barrel and is movably connected with the organic glass barrel. The baffle net and the first stainless steel net are the same in material.
Preferably, the device further comprises a first water storage barrel, a second water storage barrel, a protein separator and a microporous filter, wherein the first water storage barrel is connected with a water inlet pipeline, external seawater is stored in the first water storage barrel through rough filtration and then flows into the second water storage barrel, then part of water body sequentially enters the organic glass barrel through the microporous filter, part of water body enters the protein separator to remove protein, and the water body returns to the second water storage barrel; seawater in the organic glass barrel sequentially enters the first water storage barrel, the second water storage barrel, the microporous filter and the protein separator through the filter cotton through the water return pipeline, and then enters the organic glass barrel again, and the first water storage barrel, the second water storage barrel, the microporous filter, the protein separator and the organic glass barrel are communicated to form a circulation loop.
More preferably, the device also comprises an ultraviolet sterilizer, wherein the first water storage barrel is connected with a water inlet pipeline, external seawater is stored into the first water storage barrel through filter cotton and activated carbon coralline, then flows into the second water storage barrel, then part of water body sequentially enters the organic glass barrel through the ultraviolet sterilizer, part of water body enters the protein separator to remove protein, and returns to the second water storage barrel; seawater in the organic glass barrel sequentially enters the first water storage barrel, the second water storage barrel, the microporous filter, the ultraviolet sterilizer and the protein separator through the filter cotton through the water return pipeline, and then enters the organic glass barrel again, wherein the first water storage barrel, the second water storage barrel, the microporous filter, the ultraviolet sterilizer, the protein separator and the organic glass barrel are communicated to form a circulation loop. The second water storage barrel and the protein separator form a branch and are converged. In the invention, seawater is purified by a protein separator, then is subjected to microporous filtration, and is sterilized after impurity filtration. In the invention, the protein separator is also called a foam fractionator, and the principle that the surface of bubbles in the seawater can adsorb various granular dirt and soluble organic matters mixed in the water is utilized, an oxygenation device or a vortex pump is adopted to generate a large number of bubbles, the seawater is purified by the protein separator, all the bubbles are concentrated on the water surface to form foam, the foam adsorbed with dirt is collected in a container on the water surface, and the liquid which is later turned into turbidity is removed.
Based on the same inventive concept, the invention also provides a large-scale long-term seaweed seed preservation method based on upward flow, which comprises the following steps:
s1, after the seaweed seeds are ripe and fall, collecting a separating net arranged in an organic glass barrel, after the seaweed seeds are placed, pressing an upper opening of the organic glass barrel by using a blocking net, opening a water inlet pipeline to store water, roughly filtering external seawater by using filter cotton and storing the seawater into a first water storage barrel, and then opening a protein separator, a microporous filter, an ultraviolet sterilizer and a water inlet pipeline until the water level of the first water storage barrel is 10cm below the upper edge of the first water storage barrel, closing external water inlet, and continuously and circularly operating a seaweed seed large-scale long-term storage system;
s2, simultaneously adjusting the temperature of a cooling chamber where a large-scale long-term seaweed seed preservation system is located to 0 ℃; and opening a valve of a water outlet of each organic glass barrel every 3-4 weeks, draining water in the system, and replacing new water. Preferably, the valve of the water outlet of each organic glass barrel is opened every 3 weeks to drain the water in the system and replace new water.
Preferably, the seed preservation quantity of each organic glass barrel is 10 ten thousand grains to 100 ten thousand grains. More preferably, the seed preservation quantity of each organic glass barrel is 20 ten thousand grains to 40 ten thousand grains. Preferably, the seed storage amount of each organic glass barrel is 20 ten thousand grains, under the condition that the seed germination rate is kept at 83.1% before storage after 12 months. Preferably, the seed storage quantity of each organic glass barrel is 40 ten thousand grains, and more seeds can be stored under a certain volume of a container for storing seeds. The air pump with the air quantity of 250L/min can be used for 3 organic glass barrels. The air pump is arranged at a height of at least 10cm above the top of the organic glass barrel so as to prevent water from being sucked back into the air pump.
The sea water in the invention adopts common sea water, the salinity is 25-35 per mill, and the sea water has no special requirement on the temperature, because the sea grass seed large-scale long-term preservation system is arranged in the refrigeration house, the temperature of the sea water can be controlled by the refrigeration house. The step of searching and preserving the salinity and temperature conditions in the environment in the prior art is omitted, and the operation is simpler and more convenient.
The large-scale long-term preservation method for seaweed seeds based on the upward flow provided by the invention can realize large-scale long-term preservation of seaweed seeds. The seeds do not need complex treatment such as disinfection at all, and the operation is simple. The sea weed seed large-scale long-term preservation system based on the upward flow provided by the invention has the advantages that the retention period of the sea weed seeds is more than 12 months; after 12 months, the germination rate of the seeds still keeps 64.1-64.5% after 12 months, namely 82.5-83.1% before preservation.
The present invention will be described in detail with reference to examples.
Example 1:
as shown in fig. 1 and 2, the present embodiment provides a large-scale long-term preservation system for seaweed seeds based on upflow, which comprises 6 seed suspension devices, an air inlet pipeline 2, an water inlet pipeline 3 and a water return pipeline 4, wherein each seed suspension device comprises a plexiglass barrel 101, an air disc bracket 102 and a separation net 103. In this example, the inside diameter of the organic glass barrel 101 is 40cm, the height is 108cm, the wall thickness is 8mm, and eel seeds are put into the organic glass barrel 101 according to 20 ten thousand grains/barrel. The air inlet pipeline 2 comprises an air inlet main pipeline and a branch air inlet pipeline, wherein the diameter of the main air inlet main pipeline is 5cm, and the diameter of the branch air inlet pipeline is 1cm; the diameter of the water inlet pipeline 3 is 2.5cm, the diameter of the main pipe of the water return pipeline 4 is 5cm, and the diameter of the water discharge main pipe corresponding to the water outlet is 5cm. In this embodiment, two air pumps 5 are used to supply air to 6 organic glass barrels 101, and the air amount of the air pump 5 is 250L/min to supply air to 3 organic glass barrels 101. The annular width of the air disk support 102 is 2cm, and the width of the Chinese character 'mi' shaped support structure is 2cm. In this example, a water pump 6 with a flow rate of 9000L/h supplies 6 plexiglas barrels 101. The present embodiment employs an ultraviolet sterilizer 11 equipped with an ultraviolet sterilizer lamp having a power of 120w to adapt a circulation system composed of 6 organic glass barrels 101.
Preferably, the separation net 103 comprises a separation net support and a first stainless steel net, the first stainless steel net is formed by pressing a double-layer organic glass ring, an annular gasket is arranged on the upper portion of the first stainless steel net, the annular gasket is pressed by a plastic ring, and is fastened by screws, and the annular gasket is tightly attached to the barrel wall of the organic glass barrel 101. Preferably, a blocking net is arranged at the top of the plexiglas barrel 101. The first stainless steel net and the baffle net are 16-mesh stainless steel nets made of the same material. The organic glass ring is 2cm wide and 3mm thick. The annular gasket is a silica gel gasket, and has a width of 2.2cm and a thickness of 2mm.
As shown in fig. 3 and 4, preferably, the air disk support 102 has a plexiglas structure, the outer ring has a ring shape, and the inner part has a shape of a Chinese character 'mi'; the nanometer micropore air pipe winds a plurality of circles inside the outer ring of the air disk bracket 102 to form an air disk 104, and the air disk 104 is fixedly arranged on the air disk bracket 102.
The gas disk support 102 is arranged at a position 10cm away from the bottom of the organic glass barrel 101, the separation net 103 is arranged in the organic glass barrel 101 and is positioned above the gas disk support 102, the gas inlet pipeline 2 is communicated with the lower part of the organic glass barrel 101, the water inlet pipeline 3 is communicated with the bottom of the organic glass barrel 101 through the water outlet 106, and the water return pipeline 4 is communicated with the upper part of the organic glass barrel 101 through the water return port 107. In this embodiment, the separation net 103 is disposed 10cm above the air tray support 102.
Preferably, ropes and flexible barrel-shaped net bags for lifting seaweed seeds out of the organic glass barrel 101 are arranged on the separation net 103. Rope fixed connection is on the organic glass ring of separation net 103, the organic glass ring is fixed to be set up on the inner wall of organic glass bucket 101, and the soft chemical fiber account of texture is selected for use to the flexible barreled net of this implementation, flexible barreled net sets up in organic glass bucket 101 and turns out outside the organic glass bucket 101, first stainless steel net and fender net are located flexible barreled net and are fixed in on the inner wall of organic glass bucket 101 with flexible barreled net, flexible barreled net is used for conveniently preserving the back and collects eel grass seed. When the eel seeds are stored, or the eel seeds need to be taken out, the eel seeds are pulled out of the barrel under the combined action of the rope and the flexible barrel-shaped net bag.
Preferably, the bottom of the plexiglass tub 101 is further provided with a drain opening 105 for draining water in the plexiglass tub 101, and the drain opening 105 is located at the opposite side of the drain opening 106. The drain port 105 communicates with a drain main.
Preferably, a cylindrical filter screen is arranged on the water return port 107; an anti-overflow port is further arranged above the water return port 107. The cylindrical filter screen is formed by externally adding a stainless steel net after the strip-shaped slotting of the organic glass barrel 101, in the embodiment, the strip-shaped slotting is the slotting of the organic glass barrel 101 inserted on the water return port 107, under the scheme, after the water return port 107 is accidentally plugged, the water can enter the top opening of the first water storage barrel 7 through the anti-overflow port and the overflow pipeline, and then the seeds are recovered after being accepted by the filter cotton, so that the loss of the seeds is prevented. In this embodiment, the water return port 107 is 15cm from the top of the organic glass barrel 101, and the water overflow preventing port is 8cm from the top of the organic glass barrel 101.
Preferably, the device also comprises a first water storage barrel 7, a second water storage barrel 9, a protein separator 8, a microporous filter 10 and an ultraviolet sterilizer 11, wherein the first water storage barrel 7 is connected with the water inlet pipeline 3, external seawater is stored into the first water storage barrel 7 through filter cotton and activated carbon coralline and then flows into the second water storage barrel 9, then part of water sequentially enters the organic glass barrel 101 through the filter cotton and the activated carbon coralline, 10 and the ultraviolet sterilizer 11, part of water enters the protein separator 8 to remove protein, and the water returns to the second water storage barrel 9; seawater in the organic glass barrel 101 sequentially enters the first water storage barrel 7, the second water storage barrel 9, the microporous filter 10, the ultraviolet sterilizer 11 and the protein separator 8 through filter cotton through the water return pipeline 4, and then enters the organic glass barrel 101 again, wherein the first water storage barrel 7, the second water storage barrel 9, the microporous filter 10, the ultraviolet sterilizer 11, the protein separator 8 and the organic glass barrel 101 are communicated to form a circulation loop.
Example 2:
the difference from example 1 is that eel seeds were put into the plexiglas tub 101 in 40 ten thousand grains/tub.
Example 3:
in this embodiment, using the system for large-scale long-term preservation of seaweed seeds based on upflow described in embodiment 1, a method for large-scale long-term preservation of seaweed seeds based on upflow is provided, comprising the following steps:
s1, after eel seeds mature and fall, collecting the eel seeds on a separation net 103 in an organic glass barrel 101, after the eel seeds are placed, pressing the upper opening of the organic glass barrel 101 by using a blocking net, opening a water pump 6, opening a valve of a water inlet pipeline 3 to store water, roughly filtering external seawater by using filter cotton, storing the seawater into a first water storage barrel 7, and then opening a protein separator 8, a microporous filter 10, an ultraviolet sterilizer 11 and an air inlet pipeline 2; when the water pump 6 is turned on, the air pump 5 is turned on, the valve of the air inlet pipeline 2 is turned on, the air inlet pipeline 2 is divided into a main air inlet pipeline and a branch air inlet pipeline, air enters the branch air inlet pipelines of all barrels through the main air inlet pipeline, enters the organic glass barrel 101, and the air disk 104 formed by the nano micropore air pipe is dispersed to form ascending air flow; when the water level reaching the first water storage barrel 7 is 10cm below the upper edge of the first water storage barrel 7, external water inflow is closed, seawater in the organic glass barrel 101 enters the upper side of the first water storage barrel 7 through a water return pipeline 4 after being filtered by a barrel-shaped filter screen of a water return port 107, and after the water return port 107 is accidentally plugged, the seawater can enter the top opening of the first water storage barrel 7 through a water overflow preventing port and an overflow pipe; the first water storage barrel 7, the protein separator 8, the second water storage barrel 9, the microporous filter 10, the ultraviolet sterilizer 11 and the organic glass barrel 101 are sequentially communicated to form a circulation loop, and the seaweed seed large-scale long-term preservation system continuously and circularly operates under the action of the air pump 5 and the water pump 6;
s2, simultaneously adjusting the temperature of a cooling chamber where a large-scale long-term seaweed seed preservation system is located to 0 ℃; opening a valve of the water outlet 105 of each organic glass barrel 101 every 3 weeks, draining water in the system, and replacing new water;
s3, after the preservation of the eel seeds is finished, or when the preserved eel seeds need to be taken out, the seeds are lifted out of the barrel under the combined action of the rope and the flexible barrel-shaped net bag.
Test example:
control group: the upflow system of the embodiment 1 is not adopted for eel seed preservation, no air inlet pipeline 2, no water inlet pipeline 3 and no water return pipeline 4 are adopted, only eel seeds are put into the organic glass barrel 101 according to 20 ten thousand grains/barrel, seawater is added, the adopted seawater is consistent with the embodiment 1, the eel seeds are placed in a refrigerator at 0 ℃, and the eel seeds are kept stand for storage, so that the water body is not updated.
In the large-scale long-term preservation system of seaweed seeds based on upflow in the embodiment 1 and the embodiment 2 of the invention, the seaweed seeds are respectively stored according to the densities of 20 ten thousand grains/barrel and 40 ten thousand grains/barrel, and the quantity of the water body thalli is compared in one week. The change of the number of the bacteria in the water body measured in one week corresponds to the water changing frequency of 1-2 weeks.
Test results: as shown in FIG. 5, the seed cells stored statically in the control group were propagated in large amounts, the number of the bacteria after 1 day was 1.66 ten thousand/mL, the number was increased to 4.1 times that of the system of example 1, the water was odorized, and the number of the bacteria after 2 days was 2.21 ten thousand/mL; the number of bacteria after 5 days was 6.11 ten thousand/mL, and the number of bacteria after 7 days was 7.34 ten thousand/mL. In the large-scale long-term preservation system of seaweed seeds based on the upward flow in example 1, even if only the upward flow is opened, the ultraviolet sterilization by the ultraviolet sterilizer 11 is not performed, the number of bacteria after 1 day is 0.41 ten thousand/mL, the number of bacteria in the system of example 2 is 0.46 ten thousand/mL, the number of bacteria in the system of example 1 is 0.68 ten thousand/mL, and the number of bacteria in the system of example 2 is 0.76 ten thousand/mL; the number of bacteria in the systems of examples 1 and 2 was 0.66 and 0.80 ten thousand/mL, respectively, after 5 days, the number of bacteria in the system of example 1 was 0.54 ten thousand/mL, the number of bacteria in the system of example 2 was 0.71 ten thousand/mL, and the number of bacteria remained low for one week. It is expected that the number of bacteria will be lower in one week than the existing number provided that the uv sterilizer 11 is turned on.
As shown in FIG. 6, experiments with batches of seeds with germination rate of 83.2% before preservation show that during long-term preservation, the germination rate of the seeds stored in a control group is affected after 15 days, the germination rate is reduced to 70.1%, the germination rate is reduced to 85.3% at the beginning of preservation, the germination rate is reduced to 4.5% after 2 months, the germination rate is reduced to only 5.4% before preservation, and the seeds almost all mildew and lose vigor. After 6 months, the germination rate was 0.
In contrast, in the present invention, the germination rate of the seeds in the large-scale long-term preservation system of seaweed seeds based on the upflow in one of the embodiments 1 and 2 is still 83.2% after 15 days, the germination rate is 100% before preservation, the germination rate of the seeds in the embodiment 1 system is 82.7% after 2 months, the germination rate of the seeds in the embodiment 2 system is 99.4% before preservation, and the germination rate of the seeds in the embodiment 2 system is 81.6% and 98.1% before preservation; after 6 months, the germination rate of the seeds of the system of the example 1 is 75.3 percent, which is 90.5 percent before preservation, and the germination rate of the seeds of the system of the example 2 is 74.9 percent, which is 90.0 percent before preservation; after 12 months, the seed germination rate of the system of example 1 was 69.1%, 83.1% before preservation, and the seed germination rate of the system of example 2 was 68.6%, 82.5% before preservation.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (8)
1. The seaweed seed large-scale long-term preservation system based on the upflow is characterized by comprising a plurality of seed suspension devices, an air inlet pipeline (2), a water inlet pipeline (3) and a water return pipeline (4), wherein the seed suspension devices comprise an organic glass barrel (101), an air disc support (102) and a separation net (103), the air disc support (102) is arranged at a certain height away from the barrel bottom of the organic glass barrel (101), the separation net (103) is arranged in the organic glass barrel (101) and is positioned above the air disc support (102), the air inlet pipeline (2) is communicated below the organic glass barrel (101), the water inlet pipeline (3) is communicated with the bottom of the organic glass barrel (101) through a water inlet (106), and the water return pipeline (4) is communicated with the upper part of the organic glass barrel (101) through a water return port (107);
the device comprises a water inlet pipeline (3), a first water storage barrel (7), a second water storage barrel (9), a protein separator (8) and a microporous filter (10), wherein the first water storage barrel (7) is connected with the water inlet pipeline (3), external seawater is stored in the first water storage barrel (7) through rough filtration and then flows into the second water storage barrel (9), then part of water sequentially enters an organic glass barrel (101) through the microporous filter (10), part of water enters the protein separator (8) to remove protein, and returns to the second water storage barrel (9); seawater in the organic glass barrel (101) sequentially enters a first water storage barrel (7), a second water storage barrel (9), a microporous filter (10) and a protein separator (8) through a water return pipeline (4), and then enters the organic glass barrel (101) again, wherein the first water storage barrel (7), the second water storage barrel (9), the microporous filter (10), the protein separator (8) and the organic glass barrel (101) are communicated to form a circulation loop;
the air disc support (102) is of an organic glass structure, the outer ring is annular, and the inner part of the air disc support is shaped like a Chinese character 'mi'; the nanometer micropore trachea winds many circles in the outer lane inside of gas dish support (102), forms gas dish (104), gas dish (104) are fixed to be set up on gas dish support (102), gas dish (104) intercommunication air inlet line (2).
2. The large-scale long-term sea weed seed preservation system based on upflow according to claim 1, wherein the separation net (103) comprises a separation net support and a first stainless steel net, the first stainless steel net is formed by pressing a double-layer organic glass ring, an annular gasket is arranged on the upper portion of the first stainless steel net and is pressed by a plastic ring, and the annular gasket is tightly attached to the barrel wall of the organic glass barrel (101) through screw fastening.
3. The upflow-based seaweed seed large-scale long-term preservation system of claim 2, wherein the seed suspension device further comprises ropes and/or flexible barrel-shaped net bags for lifting seaweed seeds out of the organic glass barrel (101), the ropes are fixedly connected to organic glass rings of the separation net (103), and the organic glass rings are fixedly arranged on the inner wall of the organic glass barrel (101); the flexible barrel-shaped net is arranged in the organic glass barrel (101) and turned out of the organic glass barrel (101), and the first stainless steel and the blocking net are positioned in the flexible barrel-shaped net and fix the flexible barrel-shaped net on the inner wall of the organic glass barrel (101).
4. The large-scale long-term preservation system of seaweed seeds based on upward flow according to claim 1, wherein a drain outlet (105) for draining water in the organic glass tank (101) is further provided at the bottom of the organic glass tank (101), and the drain outlet (105) is located at the opposite side of the water inlet (106).
5. The large-scale long-term preservation system for seaweed seeds based on upflow according to claim 1, wherein an anti-overflow port is further arranged above the water return port (107).
6. The upflow-based seaweed seed large-scale long-term preservation system as claimed in claim 1, wherein a barrier net is provided on top of the plexiglas tub (101).
7. The method of using an upflow-based seaweed seed large-scale long-term preservation system as claimed in any of the claims 1 to 6, comprising the steps of:
s1, after the seaweed seeds are ripe and fall, collecting the seaweed seeds on a separation net (103) in an organic glass barrel (101), after the seaweed seeds are placed, pressing the upper opening of the organic glass barrel (101) by using a blocking net, opening a water inlet pipeline (3) to store water, roughly filtering external seawater through filter cotton and storing the seawater into a first water storage barrel (7), then opening a protein separator (8), a microporous filter (10) and an air inlet pipeline (2), and closing external water inlet when the water level of the first water storage barrel (7) is 10cm below the upper edge of the first water storage barrel (7), wherein the seaweed seeds continuously and circularly run in a large-scale long-term storage system;
s2, simultaneously adjusting the temperature of a cooling chamber where the seaweed seeds are stored in a large-scale long-term storage system to be 0 ℃; and opening a valve of a water outlet (105) of each organic glass barrel (101) every 3-4 weeks, draining water in the system, and replacing new water.
8. The method of using a large-scale long-term preservation system for seaweed seeds based on upflow as claimed in claim 7, wherein the number of seeds stored in each organic glass tank (101) is 10-100 ten thousand.
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