CN106106119A - A kind of cultural method of Monostroma nitidum Wittr. spore collection massing - Google Patents
A kind of cultural method of Monostroma nitidum Wittr. spore collection massing Download PDFInfo
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- CN106106119A CN106106119A CN201610550725.7A CN201610550725A CN106106119A CN 106106119 A CN106106119 A CN 106106119A CN 201610550725 A CN201610550725 A CN 201610550725A CN 106106119 A CN106106119 A CN 106106119A
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- Prior art keywords
- spore
- sea water
- monostroma nitidum
- nutritional solution
- massing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Life Sciences & Earth Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses the cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, specifically comprise the following steps that step one, Monostroma nitidum Wittr. pre-treatment；Step 2, spore release；Step 3, spore is collected；Step 4, collection massing method is cultivated.The present invention solves the preservation problem during Monostroma nitidum Wittr. crosses the summer；The cultural method stability of the present invention is high, and controllability is strong, efficiently solves spread required kind source problem；The cultural method of the present invention can alleviate kind of a matter degenerate problem, compares more traditional cultivation, and suspension culture is gathered easily, can use manpower and material resources sparingly, and using effect is good.
Description
Technical field
The present invention relates to the cultural method of a kind of marine algae, the cultural method of a kind of Monostroma nitidum Wittr. spore collection massing.
Background technology
Algae is Protista one class eukaryote, the most aquatic, without vascular bundle, can carry out photosynthesis.The earth
On photosynthesis 90% carried out by algae, in earth history in early days, algae plays important work in creating oxygen enriched environment
With.The algae swum is very important link in marine food chain, and all high hydrobiological existence finally rely on algae
Existence.Monostroma nitidum Wittr. is the economic algae that edibility is the highest, be grown on more the Low-salinity sea water such as estuary, neritic area rock or
On stone, the growth temperature subject range of the most sexual Monostroma nitidum Wittr. is relatively wide, is the principal item of cultivation, there is now reef in the market
Film is cultivated and cultivation successfully report, but does not occurs that facilityization cultivates successful story.The key technology of the artificial cultivation of Monostroma nitidum Wittr.
It is that the suspension collection massing of Monostroma nitidum Wittr. spore collection cultivation and Monostroma nitidum Wittr. is cultivated, the most domestic there is no perfect Monostroma nitidum Wittr. spore collection and cultivate
Technology.
Summary of the invention
It is an object of the invention to provide the cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, to solve in above-mentioned background technology
The problem proposed.
For achieving the above object, the present invention provides following technical scheme:
The cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, specifically comprises the following steps that
Step one, Monostroma nitidum Wittr. pre-treatment: select the Monostroma nitidum Wittr. that color is healthy and frond is complete, then with sterilizing seawater flushing 2-
6 times, removing the attachment on surface, be incubated at 20 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is the antiseptic sea water of 12h/12h
In and add the nutritional solution that mass fraction is 0.05%；
Step 2, spore release: in time cultivating Monostroma nitidum Wittr. and be nearly at same growth conditions, use sterile razor blade to be cut off
To 5-10mm, rinsing 3-8 time by sterile pure water, be then incubated at 25 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is 12h/
12h, seawater salinity is in 10-15ppt sterile artificial's sea water and to add the nutritional solution that mass fraction is 0.05%；
Step 3, spore collection: after spore discharges the most rapidly, (pin hole allows spore to lead to use the pipettor after wire drawing
Cross), to draw spore, be placed in the culture plate in aseptic 12 or 24 holes, adding in culture plate containing mass fraction is the battalion of 0.05%
Sterile artificial's sea water of nutrient solution, repeats 2-5 above-mentioned steps, until it reaches desired spore concentration, carries out 24-immediately after
The dark treatment of 36h；
Step 4, collection massing method cultivation: the spore after dark treatment changes fresh medium immediately, is then incubated at 20 DEG C,
Light intensity is 100 μm ol/m2s, and periodicity of illumination is in the antiseptic sea water of 12h/12h, is added with mass fraction and is in antiseptic sea water
The nutritional solution of 0.05%, treats that frond length, to more than 1mm, scrapes the algae Seedling of attachment gently with sterile scalpel, and use mesh number is
The screen filtration of 200-300 mesh, goes to algae Seedling in aseptic triangular flask, has and add 0.05% nutritional solution in aseptic triangular flask
Antiseptic sea water, aseptic triangular flask sealing ventilation is cultivated, and within every 3 days, changes a culture fluid, i.e. can get finished product.
As the further scheme of the present invention: pipettor uses high temperature wire drawing to process.
As the further scheme of the present invention: nutritional solution uses ES nutritional solution.
Compared with prior art, the invention has the beneficial effects as follows: the present invention solves the preservation that Monostroma nitidum Wittr. crosses during the summer and asks
Topic；The cultural method stability of the present invention is high, and controllability is strong, efficiently solves spread required kind source problem；The present invention
Cultural method can alleviate kind of a matter degenerate problem, compare more traditional cultivation, suspension culture is gathered easily, can use manpower and material resources sparingly,
Using effect is good.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the technical scheme of this patent is described in more detail.
Embodiment 1
The cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, specifically comprises the following steps that
Step one, Monostroma nitidum Wittr. pre-treatment: select the Monostroma nitidum Wittr. that color is healthy and frond is complete, then with sterilizing seawater flushing 2
Secondary, to remove the attachment on surface, be incubated at 20 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is the antiseptic sea water of 12h/12h
In and add the ES nutritional solution that mass fraction is 0.05%；
Step 2, spore release: in time cultivating Monostroma nitidum Wittr. and be nearly at same growth conditions, use sterile razor blade to be cut off
To 5mm, rinsing 4 times by sterile pure water, be then incubated at 25 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is 12h/12h, sea
Salinity water is in 10ppt sterile artificial's sea water and adds the ES nutritional solution that mass fraction is 0.05%；
Step 3, spore collection: after spore discharges the most rapidly, due to the positive/negative phototaxis of spore, spore can concentrate on
Side, uses the pipettor after high temperature wire drawing (pin hole allows spore to pass through), draws spore, be placed in the cultivation in aseptic 12 holes
Plate, adds the sterile artificial's sea water containing the ES nutritional solution that mass fraction is 0.05%, is repeated 2 times above-mentioned steps in culture plate,
Until reaching desired spore concentration, carry out the dark treatment of 24h immediately after；
Step 4, collection massing method cultivation: the spore after dark treatment changes fresh medium immediately, is then incubated at 20 DEG C,
Light intensity is 100 μm ol/m2s, and periodicity of illumination is in the antiseptic sea water of 12h/12h, is added with mass fraction and is in antiseptic sea water
The ES nutritional solution of 0.05%, treats that frond length, to 2mm, scrapes the algae Seedling of attachment gently with sterile scalpel, and using mesh number is 200 mesh
Screen filtration, algae Seedling is gone in aseptic triangular flask, aseptic triangular flask has add 0.05%ES nutritional solution aseptic sea
Water, aseptic triangular flask sealing ventilation is cultivated, and within every 3 days, changes a culture fluid, i.e. can get finished product.
Embodiment 2
The cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, specifically comprises the following steps that
Step one, Monostroma nitidum Wittr. pre-treatment: select the Monostroma nitidum Wittr. that color is healthy and frond is complete, then with sterilizing seawater flushing 3
Secondary, to remove the attachment on surface, be incubated at 20 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is the antiseptic sea water of 12h/12h
In and add the ES nutritional solution that mass fraction is 0.05%；
Step 2, spore release: in time cultivating Monostroma nitidum Wittr. and be nearly at same growth conditions, use sterile razor blade to be cut off
To 8mm, rinsing 6 times by sterile pure water, be then incubated at 25 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is 12h/12h, sea
Salinity water is in 12ppt sterile artificial's sea water and adds the ES nutritional solution that mass fraction is 0.05%；
Step 3, spore collection: after spore discharges the most rapidly, due to the positive/negative phototaxis of spore, spore can concentrate on
Side, uses the pipettor after high temperature wire drawing (pin hole allows spore to pass through), draws spore, be placed in the cultivation in aseptic 24 holes
Plate, adds the sterile artificial's sea water containing the ES nutritional solution that mass fraction is 0.05%, is repeated 4 times above-mentioned steps in culture plate,
Until reaching desired spore concentration, carry out the dark treatment of 28h immediately after；
Step 4, collection massing method cultivation: the spore after dark treatment changes fresh medium immediately, is then incubated at 20 DEG C,
Light intensity is 100 μm ol/m2s, and periodicity of illumination is in the antiseptic sea water of 12h/12h, is added with mass fraction and is in antiseptic sea water
The ES nutritional solution of 0.05%, treats that frond length, to 1mm, scrapes the algae Seedling of attachment gently with sterile scalpel, and using mesh number is 240 mesh
Screen filtration, algae Seedling is gone in aseptic triangular flask, aseptic triangular flask has add 0.05%ES nutritional solution aseptic sea
Water, aseptic triangular flask sealing ventilation is cultivated, and within every 3 days, changes a culture fluid, i.e. can get finished product
Embodiment 3
The cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, specifically comprises the following steps that
Step one, Monostroma nitidum Wittr. pre-treatment: select the Monostroma nitidum Wittr. that color is healthy and frond is complete, then with sterilizing seawater flushing 2
Secondary, to remove the attachment on surface, be incubated at 20 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is the antiseptic sea water of 12h/12h
In and add the ES nutritional solution that mass fraction is 0.05%；
Step 2, spore release: in time cultivating Monostroma nitidum Wittr. and be nearly at same growth conditions, use sterile razor blade to be cut off
To 10mm, rinsing 8 times by sterile pure water, be then incubated at 25 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is 12h/12h,
Seawater salinity is in 15ppt sterile artificial's sea water and adds the ES nutritional solution that mass fraction is 0.05%；
Step 3, spore collection: after spore discharges the most rapidly, due to the positive/negative phototaxis of spore, spore can concentrate on
Side, uses the pipettor after high temperature wire drawing (pin hole allows spore to pass through), draws spore, be placed in the cultivation in aseptic 24 holes
Plate, adds the sterile artificial's sea water containing the ES nutritional solution that mass fraction is 0.05%, is repeated 5 times above-mentioned steps in culture plate,
Until reaching desired spore concentration, carry out the dark treatment of 36h immediately after；
Step 4, collection massing method cultivation: the spore after dark treatment changes fresh medium immediately, is then incubated at 20 DEG C,
Light intensity is 100 μm ol/m2s, and periodicity of illumination is in the antiseptic sea water of 12h/12h, is added with mass fraction and is in antiseptic sea water
The ES nutritional solution of 0.05%, treats that frond length, to 1.5mm, scrapes the algae Seedling of attachment gently with sterile scalpel, and using mesh number is 300
Purpose screen filtration, goes to algae Seedling in aseptic triangular flask, has and add the aseptic of 0.05%ES nutritional solution in aseptic triangular flask
Sea water, aseptic triangular flask sealing ventilation is cultivated, and within every 3 days, changes a culture fluid, i.e. can get finished product
Embodiment 4
The cultural method of a kind of Monostroma nitidum Wittr. spore collection massing, specifically comprises the following steps that
Step one, Monostroma nitidum Wittr. pre-treatment: select the Monostroma nitidum Wittr. that color is healthy and frond is complete, then with sterilizing seawater flushing 2
Secondary, to remove the attachment on surface, be incubated at 20 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is the antiseptic sea water of 12h/12h
In and add the ES nutritional solution that mass fraction is 0.05%；
Step 2, spore release: in time cultivating Monostroma nitidum Wittr. and be nearly at same growth conditions, use sterile razor blade to be cut off
To 5mm, rinsing 6 times by sterile pure water, be then incubated at 25 DEG C, light intensity is 100 μm ol/m2s, and periodicity of illumination is 12h/12h, sea
Salinity water is in 15ppt sterile artificial's sea water and adds the ES nutritional solution that mass fraction is 0.05%；
Step 3, spore collection: after spore discharges the most rapidly, due to the positive/negative phototaxis of spore, spore can concentrate on
Side, uses the pipettor after high temperature wire drawing (pin hole allows spore to pass through), draws spore, be placed in the cultivation in aseptic 12 holes
Plate, adds the sterile artificial's sea water containing the ES nutritional solution that mass fraction is 0.05%, is repeated 3 times above-mentioned steps in culture plate,
Until reaching desired spore concentration, carry out the dark treatment of 27h immediately after；
Step 4, collection massing method cultivation: the spore after dark treatment changes fresh medium immediately, is then incubated at 20 DEG C,
Light intensity is 100 μm ol/m2s, and periodicity of illumination is in the antiseptic sea water of 12h/12h, is added with mass fraction and is in antiseptic sea water
The ES nutritional solution of 0.05%, treats that frond length, to more than 1mm, scrapes the algae Seedling of attachment gently with sterile scalpel, and use mesh number is
The screen filtration of 300 mesh, goes to algae Seedling in aseptic triangular flask, has the nothing adding 0.05%ES nutritional solution in aseptic triangular flask
Bacterium sea water, aseptic triangular flask sealing ventilation is cultivated, and within every 3 days, changes a culture fluid, i.e. can get finished product.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment party
Formula, in the ken that one skilled in the relevant art is possessed, it is also possible on the premise of without departing from this patent objective
Make a variety of changes.
Claims (3)
1. the cultural method of a Monostroma nitidum Wittr. spore collection massing, it is characterised in that specifically comprise the following steps that
Step one, Monostroma nitidum Wittr. pre-treatment: select the Monostroma nitidum Wittr. that color is healthy and frond is complete, then use sterilizing seawater flushing 2-6 time,
Remove surface attachment, be incubated at 20 DEG C, light intensity is 100 μm ol/m2s, periodicity of illumination be 12h/12h antiseptic sea water in also
And interpolation mass fraction is the nutritional solution of 0.05%；
Step 2, spore release: in time cultivating Monostroma nitidum Wittr. and be nearly at same growth conditions, use sterile razor blade to be cut to 5-
10mm, rinses 3-8 time by sterile pure water, is then incubated at 25 DEG C, and light intensity is 100 μm ol/m2s, and periodicity of illumination is 12h/12h,
Seawater salinity is in 10-15ppt sterile artificial's sea water and to add the nutritional solution that mass fraction is 0.05%；
Step 3, spore is collected: after spore discharges the most rapidly, use the pipettor after wire drawing (pin hole allows spore to pass through),
Drawing spore, be placed in the culture plate in aseptic 12 or 24 holes, adding in culture plate containing mass fraction is the nutritional solution of 0.05%
Sterile artificial's sea water, repeat 2-5 above-mentioned steps, until it reaches desired spore concentration, carry out 24-36h's immediately after
Dark treatment；
Step 4, collection massing method cultivation: the spore after dark treatment changes fresh medium immediately, is then incubated at 20 DEG C, light intensity
Being 100 μm ol/m2s, periodicity of illumination is in the antiseptic sea water of 12h/12h, and being added with mass fraction in antiseptic sea water is 0.05%
Nutritional solution, treat that frond length, to more than 1mm, scrapes the algae Seedling of attachment gently with sterile scalpel, using mesh number is 200-300 mesh
Screen filtration, algae Seedling is gone in aseptic triangular flask, aseptic triangular flask has add 0.05% nutritional solution antiseptic sea water,
Aseptic triangular flask sealing ventilation is cultivated, and within every 3 days, changes a culture fluid, i.e. can get finished product.
The cultural method of Monostroma nitidum Wittr. spore collection massing the most according to claim 1, it is characterised in that described pipettor uses height
Temperature wire drawing processes.
The cultural method of Monostroma nitidum Wittr. spore collection massing the most according to claim 1, it is characterised in that described nutritional solution uses ES
Nutritional solution.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718817A (en) * | 2016-12-27 | 2017-05-31 | 广东海洋大学 | A kind of a large amount of attachments of reef film zygote and cultural method |
| CN106818454A (en) * | 2016-12-28 | 2017-06-13 | 大连海宝渔业有限公司 | Reef film can be made to concentrate the method for diffusing gamete |
| CN112534042A (en) * | 2018-08-01 | 2021-03-19 | 国立大学法人高知大学 | Method for producing algal cells |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003189845A (en) * | 2001-12-27 | 2003-07-08 | Marine Biotechnol Inst Co Ltd | Method for culturing marine foliate green alga |
| CN101611693A (en) * | 2009-06-09 | 2009-12-30 | 宁波大学 | The method that a kind of seed of wild monostroma nitidum is gathered and cultivates |
| CN103392585A (en) * | 2013-07-15 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | New method for culturing kelp seedlings in scale without substratum |
| CN105684879A (en) * | 2016-04-21 | 2016-06-22 | 象山旭文海藻开发有限公司 | Method for suspension culture of chondrus ocellatus |
-
2016
- 2016-07-11 CN CN201610550725.7A patent/CN106106119A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003189845A (en) * | 2001-12-27 | 2003-07-08 | Marine Biotechnol Inst Co Ltd | Method for culturing marine foliate green alga |
| CN101611693A (en) * | 2009-06-09 | 2009-12-30 | 宁波大学 | The method that a kind of seed of wild monostroma nitidum is gathered and cultivates |
| CN103392585A (en) * | 2013-07-15 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | New method for culturing kelp seedlings in scale without substratum |
| CN105684879A (en) * | 2016-04-21 | 2016-06-22 | 象山旭文海藻开发有限公司 | Method for suspension culture of chondrus ocellatus |
Non-Patent Citations (4)
| Title |
|---|
| 李德 等: "生态因子对礁膜配子放散及黑暗时间对合子固着的影响", 《茂名学院学报》 * |
| 谢恩义 等: "宽礁膜的人工育苗及栽培", 《宽礁膜的人工育苗及栽培》 * |
| 赵素芬 等: "《海藻与海藻栽培学》", 30 September 2012, 国防工业出版社 * |
| 陈昌生: "光照对礁膜合子生长发育的影响", 《水产学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718817A (en) * | 2016-12-27 | 2017-05-31 | 广东海洋大学 | A kind of a large amount of attachments of reef film zygote and cultural method |
| CN106818454A (en) * | 2016-12-28 | 2017-06-13 | 大连海宝渔业有限公司 | Reef film can be made to concentrate the method for diffusing gamete |
| CN112534042A (en) * | 2018-08-01 | 2021-03-19 | 国立大学法人高知大学 | Method for producing algal cells |
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Application publication date: 20161116 |