CN103858746A

CN103858746A - Seaweed cultivation method

Info

Publication number: CN103858746A
Application number: CN201410116553.3A
Authority: CN
Inventors: 王鑫
Original assignee: QINGDAO LAOHAOREN MARINE BIOTECHNOLOGY Co Ltd
Current assignee: Haining Economic Development Industrial Park Development and Construction Co., Ltd
Priority date: 2014-03-27
Filing date: 2014-03-27
Publication date: 2014-06-18
Anticipated expiration: 2034-03-27
Also published as: CN103858746B

Abstract

The invention belongs to the technical field of seaweed cultivation, and relates to a seaweed cultivation method. Natural seawater is contained in a seawater tank, and the temperature and the ultraviolet lamp irradiation are controlled to conduct disinfection treatment for standby application; selected and collected seed seaweeds are washed, disinfected and dried, then conventional culture media are selected according to different seaweed types, temperature control and dim-light static cultivation are carried out, and then enzymolysis or direct cultivation is carried out; after to-be-cultivated somatic cells, to-be-cultivated gametophytes or to-be-cultivated callus mature, bolting-silk filtering, sectioning processing and/or centrifugal collecting are carried out, the somatic cells, the gametophytes or the callus are placed into the culture media according to the requirements of different kinds of seaweeds, the temperature, the illumination and the osmotic pressure are controlled for cultivation, and the somatic cells, the gametophytes/sporophores and the callus of the seed seaweeds are attached to the silk net faces in the culture media; silk nets are then manually scattered into the offshore or the tidal zone to conduct conventional seawater cultivation. The seaweed cultivation method is simple in technology, low in cost, convenient to operate, short in seedling growing time, good in cultivation effect and high in cultivation yield, and manpower is saved.

Description

A kind of algae culturing breeding method

Technical field:

The invention belongs to algae culturing technical field, be specifically related to a kind of algae culturing breeding method, according to the marine alga characteristic without kind, select tissue or protoplast etc. to realize the cultivating of tangleweed.

Background technology:

Marine alga is sea-born algae, substantially there are the kinds such as red algae, green alga, brown alga, blue-green algae, diatom and potassium algae, common are sea-tangle, asparagus and laver etc., all contain rich in protein, polysaccharose, food fiber, vitamin, inorganic elements, amino acid and fatty acid etc., there is good edibility, industrial value and medical value.In the past few decades, along with the successful Application of tissue culture technique in higher plant, the tissue of tangleweed is cultivated the concern that also starts to be subject to gradually people, but the living environment complexity of marine alga, be different from the characteristic of higher plant, make Research Advances about Tissue Culture relatively slow, the cultivation of tangleweed tissue is mainly that marine alga tissue or segment are carried out to cultured in vitro in early days, after 20th century the eighties, the tissue that is applied to tangleweed due to various new antibiotic and cleaning technique is cultivated, making many marine algas successfully realize tissue cultivates, later along with the maturation of tissue culture technique and deepening continuously of development and research, in recent decades again more carrying out cell, the research that protoplast and callus are cultivated, existing more than 60 plant tangleweed and pass through protoplast so far according to statistics, organizing stripping and slicing to realize regeneration cultivates.The method of the disclosed a kind of improved cultivation marine alga of PCT patent 00812730.1, it utilizes antibiotic and fungicide to carry out after aseptic process the marine alga material of can growing, then on the agar plate of strengthening at PES, control temperature and illumination cultivation was isolated callus after 40 days, after adjusting temperature and illumination, cultivation obtains gemmule, proceed to again liquid PES medium and under vibration condition, cultivate the rataria developing into racemosus, finally in perforated polyethylene bag, cultivate, realize marine alga large-scale culture; Chinese patent 201120053539.5 discloses a kind of ventilation type culture bottle for algae culture, it is connected with the breather pipe of an opening upwards through ventilation hole in blake bottle bottom, and the bottleneck of blake bottle is provided with filter membrane, be convenient to passing into of gas, improve the success rate of algae culture; But disclosed algae culturing cultural method and device in above-mentioned patented technology, all there is complex process, condition is difficult to control, required cost of investment is high, the shortcomings such as hand labor intensity is large, therefore the present invention seeks to design a kind of easy enforcement, speed is fast, efficiency is high algae culturing breeding method, has a good application prospect and implementary value.

Summary of the invention:

The object of the invention is to overcome the shortcoming that prior art exists, seek design a kind of algae culturing breeding method is provided, cultivate for the rapid continuous cultivation of carrying out of variety classes marine alga, to improve algae culturing output.

To achieve these goals, the concrete technology step of the algae culturing cultural method of the present invention's design is:

(1) nature seawater is packed in seawater tank, control temperature 70-90 DEG C, ultraviolet lamp irradiation is carried out heat sterilization for 2-5 hour and is processed rear for subsequent use;

(2) clean, sterilize and be dried processing to reduce cell moisture content selecting the kind marine alga of collection, then select to put into conventional medium according to different marine alga kinds and comprise that PESI medium, PES medium, MES medium, VAS medium, ERD medium or the static cultivation of 15-20 DEG C of low light level of ESS medium temperature control are after 4-15 days, or according to variety classes marine alga need to carry out enzymolysis processing, or control temperature and illumination and directly carry out second incubation;

(3) after somatic cell, gametophyte or the callus maturation of cultivating in step (2), carry out silk cover filtering, segment processing and/or centrifugal collection, then according to the needs of variety classes marine alga, put into the medium that routine contains the seawater of sterilizing, control 15-28 DEG C of temperature, illumination 500-4000Lx and osmotic pressure, cultivate 10-20 days, somatic cell, gametophyte/sporophyte, the callus of kind of marine alga are attached on the gauze face in medium;

(4) manually step (3) is sprinkled into coastal waters or tidal zone with the gauze of kind of a marine alga somatic cell, gametophyte/sporophyte, callus, carries out mariculture, between culture period, net is regularly applied fertilizer, shone to cultural method routinely.

The present invention compared with prior art, can be widely used in all kinds of algae culturings and cultivate, and to improve the output of marine alga, its method technique is simple, with low cost, easy to operate, saves manpower, and seedling raise period is short, and culture efficiency is good, and cultivation output is high.

Embodiment:

Be described further below by embodiment.

Embodiment 1:

The present embodiment describes as an example of laver cell cultivation example, and concrete grammar comprises following processing step:

(2) be dried and process to cell moisture content below 20% selecting the main laver of collection, in medium, the static cultivation of the temperature control 15-20 DEG C low light level is placed in the enzyme liquid that contains sea snail enzymes, abalone enzyme and agarase for 4-5 days, temperature control 23-28 DEG C, pH-value 5.6-6.4, carry out enzymolysis processing, then use silk cover filtering enzyme liquid and the cell in 30-60um aperture, and centrifugal collection main laver somatic cell is stand-by;

(3) the main laver somatic cell of collecting in step (2) is placed in and contains the conventional 2-(N-morpholino of (1) sterilization seawater in steps) ethyl sulfonic acid (MES) medium, control temperature 19-22 DEG C, osmotic pressure 1.02-1.03mol/L, illumination 500-3000Lx, cultivate after 10-16 days, main laver somatic cell is attached on the gauze face in medium;

(4) manually will be sprinkled into coastal waters or tidal zone with the somatic gauze of main laver, and carry out mariculture, between culture period, net is regularly applied fertilizer, shone to cultural method routinely.

Embodiment 2:

The present embodiment describes as an example of kelp gametophyte cultivation cultivation example, and concrete grammar comprises following processing step:

(2) select frond generous, be rich in sea-tangle that gloss, stalwartness and sporangiorus area are large as main laminaria, with after the seawater flushing main laminaria 3-5 after sterilization in step (1) time, be placed in and contain the conventional synthetic medium of the rear seawater of (1) sterilization in steps, control temperature 15-19 DEG C, day illumination 500-4000Lx, cultivated after 6-10 days, formed trip spore, improve temperature and after 5-10 days, form egagametophyte and male gametophyte to 18-21 DEG C of cultivation;

(3) temperature is adjusted to 10-15 DEG C, adopt long illumination, continue to cultivate 5-10 days, female, male gametophyte forms spherical or bunch shape trophosome through vegetative propagation, expand to cultivate after algae ball or algae number of clusters amount are increased and be cut into cellule section, broken section of female, male gametophyte is attached on the gauze of Seedling culturing device in medium;

(4) cultivate 10-15 days for temperature control 16-19 DEG C; female, male gametophyte on gauze is ripe and carry out sexual propagation and produce zygote gradually; zygote is sprouted into juvenile sporophyte again; then manually will be sprinkled into coastal waters or tidal zone with the lace curtaining of main laminaria juvenile sporophyte; carry out mariculture, between culture period, net is regularly applied fertilizer, shone to cultural method routinely.

Claims

1. an algae culturing breeding method, is characterized in that concrete technology step is:

(2) clean, sterilize and be dried processing to reduce cell moisture content selecting the kind marine alga of collection, then select to put into the 15-20 DEG C of static cultivation of low light level 4-15 days of conventional medium temperature control according to different marine alga kinds, again according to the needs of variety classes marine alga or carry out enzymolysis processing, or control temperature and second incubation is directly carried out in illumination;