TWI611755B - Seaweed seeding cultivation substrate and method thereof - Google Patents

Abstract

The method for artificially cultivating seaweed seedlings comprises: impregnating a plurality of seaweed fertilized eggs on a matrix body, wherein the matrix body has a plurality of fiber gaps; and the plurality of seaweed fertilized eggs are artificially cultivated for a first predetermined time for germination to form a plurality of seaweed seedlings; after the seaweed fertilized eggs release a plurality of attached filaments, the attached filaments are entangled and attached to the fiber gap; and the plurality of seaweed seedlings are further artificially cultivated for a second predetermined time, so as to form germination A seaweed seedling. In addition, when transplanting the seaweed seedlings, it is selected to directly cut the matrix body to form a tailored matrix unit.

Description

Seaweed seedling artificial cultivation substrate and method thereof

The present invention relates to a seaweed seeding cultivation medium and a method thereof; in particular, to a seaweed seedling artificially cultivating an adherent substrate and a method thereof.

The artificial seaweed seedling artificial breeding rope group and the manufacturing method thereof, for example, the invention patent of the self-adhesive seaweed seedling group production method disclosed in the Republic of China Announcement No. I339099, discloses a self-adhesive seaweed seedling group production method. The method comprises: a. First, selecting a rope group, and placing the rope group in an aqueous seawater solution, and first boiling the rope group for 30 minutes, and then replacing the new seawater solution to re-boil the rope group, and Repeating the above-mentioned operation of replacing seawater and re-boiling to uniformly adhere a salt to the rope group; b, and then drying the rope group at a drying temperature of 100 ° C to 130 ° C to dry the rope set Forming a dried rope group, and storing the salt in the dried rope group; c. Next, mixing the dried rope group with a seaweed glue to obtain an algae mixed rope group, and The algae-mixed rope group is subjected to an autoclave treatment to obtain a sterilized rope group; d. Finally, the sterilized rope group is subjected to air-drying treatment.

According to the above, the autoclave treatment of the above No. I339099 adopts a pressure value of 10 bar to 20 bar, and the autoclave treatment time is selected to be 10 minutes to 30 minutes; the air drying treatment is selected to be placed at room temperature. And choose to naturally dry for 24 hours to 72 hours to form a self-adhesive seaweed seedling group.

According to the above, the rope set of the aforementioned No. I339099 is selected by one Made of PVC material, and the rope set is made into a mesh shape. The aqueous seawater solution has a predetermined salt content, and the predetermined salt content is a 3 ppt value [thousands of grams]. The algin is selected from a 4% to 8% sodium alginate and a 90% or more RO water. The algin glue is selected from a brown algae, an agar, an amaranth, a carrageenan, a red algae (Furcellarin), a paste (Funoran) and or a laver (Porphyra).

However, the conventional seaweed seedling artificial breeding rope group and the manufacturing method thereof have the disadvantages of considerable process complexity. In short, the conventional seaweed seedling artificial breeding rope group and its manufacturing method necessarily have the potential need to further provide a simplified artificial algae seedling cultivation substrate and a manufacturing method thereof. The above-mentioned Republic of China Announcement No. I339099 is merely a reference to the technical background of the present invention and a state of the art development, which is not intended to limit the scope of the present invention.

In view of the above, in order to meet the above technical problems and needs, the present invention provides a seaweed seedling artificial growth substrate and a method thereof, which are characterized in that several seaweed fertilized eggs are impregnated and attached to a matrix body, and the matrix body has several fiber gaps. In order to artificially cultivate a plurality of seaweed seedlings, and after the seaweed fertilized eggs release a plurality of attached silks (seedling roots), the attached filaments are entangled and attached to the fiber gaps, thereby improving the artificially cultivated rope group of the conventional seaweed seedlings and The manufacturing method has a technical problem of complicated processes.

The main object of the present invention is to provide a seaweed seedling artificial breeding substrate and a method thereof, which are characterized in that several seaweed fertilized eggs are impregnated and attached to a matrix body, and the matrix body has several fiber gaps for artificially cultivating several seaweed seedlings. After the fertilized egg of the seaweed releases a plurality of attached filaments (seedling roots), the attached filament is wound and attached to the fiber gap to achieve the purpose of simplifying the artificial culture substrate structure of the seaweed seedling and the manufacturing method thereof.

In order to achieve the above object, a method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention comprises: impregnating a plurality of seaweed fertilized eggs on a matrix body, and The matrix body has a plurality of fiber gaps; the plurality of seaweed fertilized eggs are artificially cultivated for a first predetermined time to germination to form a plurality of seaweed seedlings; after the seaweed fertilized eggs release a plurality of attached filaments, the attached filaments are entangled and attached On the gap of the fiber; and the plurality of seaweed seedlings are further artificially cultivated for a second predetermined time to germinate to form a plurality of seaweed seedlings.

In a preferred embodiment of the invention, when the seaweed seedling is transplanted, the matrix body is selected to be directly cut to form a tailored matrix unit.

In the preferred embodiment of the invention, the matrix body has a plurality of cutting lines for cutting the matrix body along the cutting line.

The matrix body of the preferred embodiment of the invention is recovered as a reusable matrix body.

The tailored matrix unit of the matrix body of the preferred embodiment of the invention is recovered as a reusable matrix unit and the reusable matrix unit can be re-spliced.

In order to achieve the above object, the seaweed seedling artificial growth substrate of the preferred embodiment of the present invention comprises: a matrix body comprising a plurality of fiber filaments and at least one algae growth surface, and the plurality of fiber filaments are woven to form the matrix body; a fiber gap formed between the filaments of the matrix body; and a plurality of seaweed fertilized eggs, which are impregnated and attached to the filaments of the matrix body; wherein after the fertilized egg of the seaweed releases a plurality of attached filaments, The attached filament is wound and attached to the fiber gap, and the plurality of seaweed fertilized eggs are artificially cultivated for a first predetermined time to germination to form a plurality of seaweed seedlings, and the plurality of seaweed seedlings are further artificially cultivated for a second predetermined time. In order to germinate to form several seaweed seedlings.

In the preferred embodiment of the invention, the fiber filament of the matrix body is made of a linear polymer, a polyester fiber or a high density polyester fiber.

In the preferred embodiment of the invention, the fiber filaments of the matrix body are a linear intertwined braid or a mesh cross-woven fabric.

In the preferred embodiment of the present invention, the growth surface of the algae seedling is a top surface, a side surface of the surface or other algae growth surface that can illuminate the light.

In the preferred embodiment of the present invention, the shape of the matrix body is a disc body, a square block, a long block or other shape block.

1‧‧‧Matrix

1a‧‧‧Matrix

10‧‧‧Cutting matrix unit

11‧‧‧Fiber

11a‧‧‧Linear intertwined braid

11b‧‧‧ mesh crossover fabric

110‧‧‧Fiber gap

111‧‧‧ cutting line

2‧‧‧ seaweed fertilized eggs

21‧‧‧ Attached wire

3‧‧‧ farming containers

Fig. 1 is a schematic view showing the flow of a method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention.

Fig. 2 is a schematic view showing the artificially cultivated substrate of seaweed seedlings combined with seaweed fertilized eggs according to the first preferred embodiment of the present invention.

Fig. 3 is a schematic view showing the artificially cultivating substrate of seaweed seedlings combined with seaweed fertilized eggs according to the second preferred embodiment of the present invention.

Fig. 4 is a schematic view showing the artificially cultivating a cutting substrate unit of the artificially cultivated substrate of the seaweed seedling in the second preferred embodiment of the present invention.

Fig. 5 is a schematic view showing the growth of the seedlings of the seaweed fertilized eggs by the seaweed seedling artificially cultivating substrate according to the second preferred embodiment of the present invention.

6(A) to 6(C): The artificial culture method of seaweed seedlings according to a preferred embodiment of the present invention is used to culture a series of images of Sargassum.

7(A) to 7(C): The artificial culture method of seaweed seedlings according to a preferred embodiment of the present invention is used for cultivating a series of images of S. cerevisiae.

In order to fully understand the present invention, the preferred embodiments of the present invention are described in detail below, and are not intended to limit the invention.

The seaweed seedling artificial culture substrate structure, the manufacturing method thereof and the artificial breeding method thereof according to the preferred embodiment of the present invention can replace the seaweed collection in the field, and are suitable for artificially cultivating various seaweed seedlings, for example: Sargassum cristaefolium , thick Sargassum crassifolium , Sargassum hemiphyllum var. chinense , Sargassum polycystum , Sargassum siliquosum , Sargassum glaucescens, and crown leaves Sargassum crispifolium , but it is not intended to limit the scope of application of the present invention.

The seaweed seedling artificial culture substrate structure, the manufacturing method thereof and the artificial cultivation method thereof according to the preferred embodiment of the present invention are applicable to: the first large algae body contains chlorophyll a (Chlorophyll a) and fucoxanthin (Fucoxanthin) components, in classification It belongs to the genus Chromista, Ochrophyta, Phaeophyceae, and has multicellular algae with attachments (or anchors); the second largest species contains chlorophyll a and Chlorella Phycobiliprotein component, which belongs to the plant kingdom (Plantae), the red alga plant plant (Phodophyta), and has multicellular algae with attachments; the third largest algae body contains chlorophyll a component, which belongs to the plant kingdom in classification. , Chlorophyta, and multicellular algae with attachments.

1 is a schematic flow chart showing a method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention; and FIG. 2 is a schematic view showing the artificial breeding substrate of seaweed seedlings combined with seaweed fertilized eggs according to the first preferred embodiment of the present invention. Referring to Figures 1 and 2, the artificially cultivated substrate of seaweed seedlings according to the first preferred embodiment of the present invention comprises a matrix body 1, a plurality of fiber clearances 110 and a plurality of seaweed fertilized eggs (zygote) 2.

Referring to FIG. 2 again, for example, the shape of the matrix body 1 is a disk body, a square block, a long block or other shape block, such as a hemispherical block or a spherical block. Body, and the matrix body 1 comprises a plurality of filaments 11 and at least one algae growth surface, for example For example, the growth surface of the algae seedling is a top surface, a side surface or other growth surface of the algae that can illuminate the light. The filaments 11 of the matrix body 1 are a linear intertwined braid 11a as shown by the long black lines of Fig. 2.

Referring to FIG. 2 again, for example, the plurality of filaments 11 are woven to form the matrix body 1, and the filaments 11 of the matrix body 1 are composed of a linear macromolecule polymer. Made of ester or a high-density polyester fiber, and it has biological growth or physiological significance for the seaweed fertilized egg 2. Further, the plurality of fiber slits 110 are formed between the filaments 11 of the matrix body 1 to accommodate the plurality of seaweed fertilized eggs 2 by the plurality of fiber slits 110.

In another preferred embodiment of the present invention, the matrix body 1 further comprises a reinforcing fiber filament or the like, and the reinforcing fiber filament is bonded between the plurality of fiber filaments 11 and the reinforcing fiber filament The fiber filament 11 does not affect the growth or physiological significance of the seaweed fertilized egg 2.

Please refer to FIG. 2 again. For example, the preparation of the fertilized egg 2 of the seaweed can be selected from the field to select the mature S. cerevisiae with the reproductive support. The S. cerevisiae is to be kept in a clean marine aquaculture tank, and the ratio of the volume of the culture water to the total volume of the algae is 10:1 or other ratio, and the selected gas output can be selected to be pumped into the culture tank. And the optional air outlet output can be maintained at 1.2L/min. In addition, the luminaire is placed above the breeding barrel, and the color lamp can be selected from a color temperature of 2700K to 3000K (warm white light) LED lamp, and the light intensity of the horizontal plane is 100 to 150 μmol photons m ^-2 s ^-1 . Under the previous dry treatment and strong light stimulation, the reproductive organs of the male and female S. cerevisiae are separately released from the sperm and eggs, and fertilized in the aquaculture water.

Please refer to Fig. 2 again. For example, the S. cerevisiae stimulated by strong light is taken out from the culture barrel every 30 minutes and temporarily placed in a cool place. Next, the 50 μm plankton net was used to harvest the fertilized eggs of S. cerevisiae in the water. Next, the harvested S. cerevisiae fertilized egg Temporarily placed in a seawater storage tank.

Fig. 3 is a schematic view showing the artificially cultivated substrate of seaweed seedlings combined with seaweed fertilized eggs according to the second preferred embodiment of the present invention, which corresponds to the artificially cultivated substrate of seaweed seedlings of Fig. 2. Referring to FIG. 3, the artificial culture substrate of the seaweed seedling of the second preferred embodiment of the present invention comprises a matrix body (or an adherent matrix body) 1a, and the fiber filament 11 of the matrix body 1a is compared with the first embodiment. The braid 11b is crossed to each other in a mesh shape to strengthen the overall structure.

Referring to FIG. 3 again, the matrix body 1a of the second preferred embodiment of the present invention further has a plurality of cutting lines 111 (such as the dotted line shown in FIG. 3) or lines having similar functions, so as to Different requirements may be used to tailor the substrate 1a along the cutting line 111 on subsequent cutting or other processes.

Referring to Figures 1, 2 and 3 again, the method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention comprises the step S1: first, the plurality of seaweed fertilized eggs 2 after collection are appropriately subjected to various impregnation methods (for example: Soaking, spreading, coating, spraying or other suitable technical means are attached to the matrix body 1, such as the gray dots shown in Figures 2 and 3.

Figure 4 is a schematic view showing the artificially cultivating a cutting substrate unit of the artificially cultivated substrate of the seaweed seedling in the second preferred embodiment of the present invention. Referring to Figure 4, the matrix body 1a is cut to form a cutting matrix unit 10, and the cutting substrate unit 10 is placed in a culture vessel 3 or the like.

Referring again to Figures 1, 2, 3 and 4, for example, the matrix body 1, 1a [length 40 cm × width 30 cm × height 1.5 cm] is fixed in a transparent plastic case [length 40 cm × width 30 cm × The bottom of the height of 15 cm], and the collected heavy-edge leaf ponytail fertilized egg aqueous solution is uniformly dispersed on the matrix body 1, 1a. Next, fresh seawater is added to the substrate body 1, 1a 5 cm or other suitable height, and a seawater culture solution containing 1 ppm Provasoli's enriched seawater [PES] and having 30 to 35psu salinity.

Referring to Figures 1, 2 and 3 again, the method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention comprises the step S2: subsequently, the plurality of seaweed fertilized eggs 2 are artificially cultivated for a first predetermined time under appropriate breeding conditions. In order to germinate to form several seaweed seedlings.

Referring again to Figures 1, 2 and 3, for example, the matrix body 1, 1a is placed in a constant temperature 25 ± 2 ° C culture chamber, and provides an illumination intensity of 50 to 100 μmol photons m ^-2 s ^- The light source of ^{1 has a} photoperiod of 12 hours of light and 12 hours of light and darkness. During the culture, it is administered as a gas, and the gas output of the air compressor is maintained at 0.5 L/min.

Fig. 5 is a view showing the growth of the algae fertilized eggs of the seaweed seedling artificially cultivating substrate according to the second preferred embodiment of the present invention. Referring to Figures 1, 2, 3 and 5 again, the method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention comprises the step S3: Next, a plurality of attached filaments (seedling roots) are released in the fertilized egg 2 of the seaweed 21 Thereafter, the attached wire 21 is wound and attached to the fiber slit 110, and the filament 11 has biological growth or physiological significance to the attached silk 21 of the seaweed fertilized egg 2.

Referring to Figures 1, 2, 3 and 5 again, the method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention comprises the step S4: subsequently, the plurality of seaweed seedlings are continuously cultured under suitable breeding conditions for a second predetermined Time to germinate to form several seaweed seedlings.

Referring to Figures 1, 2, 3 and 4 again, the method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention further comprises the steps of: directly cutting the matrix body 1, 1a when transplanting the seaweed seedlings, The tailored substrate unit 10 is formed as shown in FIG.

Referring to Figures 1, 2, 3 and 5 again, on the 15th day after the start of the culture, the growth of the S. cerevisiae seedlings was observed to be about 0.05 to 0.09 cm. The seedlings of S. cerevisiae can grow from about 0.2 cm to 0.4 cm when cultured for 25 days. At this time, the present invention may choose to attach the Sargasso seedlings The substrate is cut to size and moved to the outer sea or outdoor pool for further cultivation.

Figures 6(A) to 6(C) show a series of images of a seaweed seedling artificial cultivation method for cultivating a species of S. cerevisiae according to a preferred embodiment of the present invention. Please refer to Figure 6(A) for the first time, after fertilization, the image of S. cerevisiae eggs (Bar = 200 μm). Please refer to Fig. 6(B), and then, the image of the attached silk is released by Barnella gracilis [Bar = 200 μm]. Please refer to Figure 6(C) for the image of the seedlings of S. cerevisiae (Bar=500μm).

7(A) to 7(C) are diagrams showing a method for artificially cultivating seaweed seedlings according to a preferred embodiment of the present invention for cultivating a series of images of S. cerevisiae, which corresponds to a series of images of Fig. 6. Please refer to Figure 7(A) for the image of the eight-splitting stage of fertilized eggs of Sargassum serrata [Bar = 200 μm]. Please refer to Figure 7(B) for the image of the seedlings of the fertilized egg of S. cerevisiae (Bar=200μm). Please refer to Fig. 7(C) for the image of seedlings germinated on the 25th day of culture of S. cerevisiae (Bar = 0.05 mm).

The foregoing preferred embodiments are merely illustrative of the invention and the technical features thereof, and the techniques of the embodiments can be carried out with various substantial equivalent modifications and/or alternatives; therefore, the scope of the invention is subject to the appended claims. The scope defined by the scope shall prevail. The copyright limitation of this case is used for the purpose of patent application in the Republic of China.

1‧‧‧Matrix

11‧‧‧Fiber

11a‧‧‧Linear intertwined braid

110‧‧‧Fiber gap

2‧‧‧ seaweed fertilized eggs

Claims (10)

A method for artificially cultivating seaweed seedlings comprises: impregnating a plurality of seaweed fertilized eggs on a matrix body, wherein the matrix body has a plurality of fiber gaps; and artificially cultivating the plurality of seaweed fertilized eggs for a first predetermined time so that Germinating forms a plurality of seaweed seedlings; after the seaweed fertilized eggs release a plurality of attached filaments, the attached filaments are entangled and attached to the fiber gap; and the plurality of seaweed seedlings are further artificially cultivated for a second predetermined time for germination Several algae seedlings are formed. The method for artificially cultivating seaweed seedlings according to claim 1, wherein when the seaweed seedlings are transplanted, the matrix body is directly cut to form a tailored matrix unit. The method for artificially cultivating seaweed seedlings according to claim 1, wherein the matrix body has a plurality of cutting lines for cutting the matrix body along the cutting line. The method for artificially cultivating seaweed seedlings according to claim 1, wherein the matrix body is recovered as a reusable matrix body. The method for artificially cultivating seaweed seedlings according to claim 2, wherein the cutting matrix unit of the matrix body is recovered as a reusable matrix unit, and the reusable matrix unit can be re-spliced. A seaweed seedling artificial growth substrate comprising: a matrix body comprising a plurality of fiber filaments and at least one algae growth surface, and the plurality of fiber filaments are woven to form the matrix body; and a plurality of fiber slits formed on the matrix Between the filaments of the body; and a plurality of seaweed fertilized eggs, which are impregnated and attached to the filaments of the matrix body; wherein after the fertilized eggs of the seaweed release a plurality of attached filaments, the attached filaments are entangled and attached to the fiber gap And then, the plurality of seaweed fertilized eggs are artificially cultivated for a first predetermined time to germination to form a plurality of seaweed seedlings, and then the plurality of The seaweed seedlings continue to be artificially cultivated for a second predetermined time to germinate to form several seaweed seedlings. The artificially cultivated substrate of the seaweed seedling according to the sixth aspect of the patent application, wherein the fiber filament of the matrix body is made of a linear polymer, a polyester fiber or a high density polyester fiber. The artificially cultivated substrate of the seaweed seedlings according to the sixth aspect of the patent application, wherein the fiber filaments of the matrix body are a linear intertwined braid or a mesh cross-woven fabric. According to the sixth aspect of the patent application, the seaweed seedling artificial cultivation substrate, wherein the growth surface of the algae seedling is a top surface, a side surface or an algae growth surface that can illuminate the light. The artificially cultivated substrate of the seaweed seedling according to the sixth aspect of the patent application, wherein the shape of the matrix body is a disc body, a square block, a long block or a spherical block.