CN105850747B - A kind of jade of the tissue rapid propagation method of the jade of succulent rainbow and its rainbow of culture - Google Patents
A kind of jade of the tissue rapid propagation method of the jade of succulent rainbow and its rainbow of culture Download PDFInfo
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- CN105850747B CN105850747B CN201610317835.9A CN201610317835A CN105850747B CN 105850747 B CN105850747 B CN 105850747B CN 201610317835 A CN201610317835 A CN 201610317835A CN 105850747 B CN105850747 B CN 105850747B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The present invention relates to a kind of tissue rapid propagation method of jade of succulent rainbow and its jade of the rainbow of culture, belong to field of plant tissue culture technique.The main technical schemes of the present invention are as follows：The selection and sterilization of explant：Using the beautiful blade or stem section of rainbow as explant, by the clean explant of surface clean with alcohol-pickled, then soaked with mercuric chloride solution to obtain aseptic explant；Aseptic explant is inoculated in induction Multiple Buds culture medium, culture can obtain intact plant in 23 weeks.The beautiful seedling that the present invention obtains rainbow by carrying out tissue cultures to the beautiful blade or stem section of rainbow, this method material is easy to get, sterilization is convenient, survival rate is high, it is with short production cycle, the breeding coefficient and reproduction speed of the jade of rainbow are improved, and can preferably keep the strain advantage of the jade of rainbow, the beautiful seedling of a large amount of excellent rainbows for being adapted to transplanting can be quickly bred, meets production requirement.
Description
Technical field
The present invention relates to a kind of rapid propagation method of plant tissue, specially a kind of tissue of the jade of succulent rainbow is fast
The jade of fast propagation method and its rainbow of culture, belongs to field of plant tissue culture technique.
Background technology
Rainbow beautiful (Sedum rubrotinctum) is the succulent of Crassulaceae Sedum, and perennial meat draft is planted
Thing, the speed of growth is than very fast, and spring and summer is the main season of growth, and 35 degree of high temperature of summer need to shelter from heat or light, 10-20 centimetres of its plant height, more points
Branch, fleshy leaf expand alternate, cylindrical shape to avette, long 2 centimetres, green, and epidermis is bright, without white powder, in sun-drenched condition
Under switch to bronzing, the yellowish red of little Hua, form is lovely, deep to be liked by everybody, and ornamental value is high.The culture of the jade of rainbow is generally adopted
Cuttage is taken, i.e., intact leaf is removed from stem, cuttage again after placing 3 days, the method that row breeding is injected using leaf, although breeding
Coefficient is big, but shaping is slower, and growth period length, there is presently no the correlation technique that the jade on rainbow is quickly bred.
The content of the invention
In view of the shortcomings of the prior art, the present invention provide a kind of red jade of succulent tissue rapid propagation method and its
The jade of the rainbow of culture, this method have the characteristics of easy to operate, cultivation cycle is short, survival rate is high.
Technical scheme is as follows：A kind of tissue rapid propagation method of the jade of succulent rainbow, this method include
Following steps：(1) selection and sterilization of explant：Using the beautiful blade or stem section of rainbow as explant, by clean outer of surface clean
Implant soaks 3-10 minutes with 70vt%-75vt% alcohol-pickled 20-30 seconds, then with 0.1wt% mercuric chloride solution, often
It is secondary soaked after with sterile water washing, obtain aseptic explant；
(2) induction of Multiple Buds：Aseptic explant is inoculated in induction Multiple Buds culture medium, done with the stem section of the jade of rainbow
During explant, inoculated after stem section first to be cut into 1cm or so segment in culture medium, in 23-26 DEG C, intensity of illumination 800-
1500lux, light application time be 24 hours/day, cultivate 3-7 days in the environment of relative humidity 55%-65% after explant understand Mr.
Root, Multiple Buds are obtained after continuing culture 3-5 days, induction Multiple Buds culture medium is using MS culture mediums as minimal medium, to every liter
0.6-1.5mg 6-benzyladenines, 0.6-1.5mg heteroauxins, 25-30g sucrose, 8.0-8.4g are added in minimal medium
Agar, 3-7g activated carbons, NaOH regulation pH value is 5.80-6.0, is preferably：1.0mg 6-benzyladenines, 1.0mg indoles second
Acid, 30g sucrose, 8.0g agar, 5g activated carbons, NaOH regulation pH value is 5.82；
(3) subculture：The young shoot for the Multiple Buds that step (2) culture is obtained, which is cut, to be transferred in subculture medium, cultivates 2-3
Week can obtain intact plant；Subculture medium is using MS culture mediums as minimal medium, is added into every liter of minimal medium
1.0mg 6-benzyladenines, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, 5g activated carbons, pH value 5.82.
(4) transplant：The culture medium remained on the intact plant obtained through squamous subculture its root is rinsed well, is placed in cool place
Transplanting shade, grows into sand soil in the case where temperature is 18-26 DEG C, relative humidity is 55%-65% after place air-dries.
The jade for the succulent rainbow for cultivating to obtain according to the method described above is claimed in the present invention simultaneously.
Beneficial effects of the present invention are as follows：The present invention obtains rainbow by carrying out tissue cultures to the beautiful blade or stem section of rainbow
Beautiful seedling, this method material is easy to get, and sterilization is convenient, and survival rate is high, with short production cycle, improve rainbow jade breeding coefficient with
Reproduction speed, and the strain advantage of the jade of rainbow can be preferably kept, it can quickly breed the jade of a large amount of excellent rainbows for being adapted to transplanting
Seedling, meet production requirement；The charcoal absorption that induction Multiple Buds culture medium in the present invention with the addition of suitable concentration is harmful to
Brown stain of the material not only to explant has certain inhibitory action, and then improves the breeding coefficient of the jade of rainbow, breeding speed
Degree and survival rate of plant, improve the economic benefit of grower.
Brief description of the drawings
Fig. 1 is the photo that the blade that embodiment 1 obtains is taken root；
Fig. 2 is the photo for the Multiple Buds that embodiment 2 obtains；
Fig. 3 is the intact plant that embodiment 1 is transplanted in soil；
Fig. 4 is the photo of comparative example blade；
Fig. 5 is the photo that brown stain occurs for comparative example blade.
Embodiment
The present invention is described in more detail with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited only to
These embodiments, unless otherwise specified, the present invention are raw materials used commercially available.
Embodiment 1
In the present embodiment：It is using MS culture mediums as minimal medium, in every liter of minimal medium to induce Multiple Buds culture medium
1.0mg 6-benzyladenines, 1.0mg heteroauxins, 30g sucrose, 8.0g agar are added, 5g activated carbons are formulated, then are used
NaOH regulation pH value is 5.82；
Subculture medium is using MS culture mediums as minimal medium, and 1.0mg6- benzyls are added into every liter of minimal medium
Adenine, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, 5g activated carbons, then it is 5.82 to adjust pH value with NaOH.
(1) selection and sterilization of explant
The beautiful plant of healthy rainbow is chosen, plant surface soil is eluted with water and is rinsed 15 minutes with flowing water again.In ultra-clean work
First with the beautiful plant 30 seconds for the alcohol-pickled rainbow that volume fraction is 75% on platform, then with sterile water washing 3 times, every time 2 minutes；
Take the blade of the jade of rainbow that explant is transferred in the mercuric chloride solution that mass fraction is 0.1% and soaks 3 points as explant
Clock, then with sterile water washing 6 times, 2 minutes every time, obtain aseptic explant；
(2) induction of Multiple Buds
Aseptic explant is inoculated in the induction Multiple Buds culture medium in tissue culture bottle, every bottle is inoculated with 4, in 25 DEG C, light
According to intensity 1200lux, light application time be 24 hours/day, cultivate 6 days in the environment of relative humidity 55% after explant take root, after
Continuous culture is germinateed after 3 days obtains Multiple Buds；By above-mentioned 9 days Fiber differentiations, the beautiful blade of rainbow induced synthesis Multiple Buds；
(3) subculture：Multiple Buds are accessed in subculture medium and continue to cultivate 3 weeks intact plants taken root；
(4) transplant：It will be rinsed well with the culture medium remained on intact plant root, In Shade to air-dry 1 day, transplanting is extremely
In sand soil, shade, temperature be 22 DEG C, relative humidity be 55% time grow.
Embodiment 2
In the present embodiment：It is using MS culture mediums as minimal medium, in every liter of minimal medium to induce Multiple Buds culture medium
0.6mg 6-benzyladenines, 0.6mg heteroauxins, 25g sucrose, 8.0g agar are added, 3g activated carbons are formulated, then are used
NaOH regulation pH value is 5.80；
Subculture medium is using MS culture mediums as minimal medium, and 1.0mg6- benzyls are added into every liter of minimal medium
Adenine, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, 5g activated carbons, then it is 5.82 to adjust pH value with NaOH.
(1) selection and sterilization of explant
The beautiful plant of healthy rainbow is chosen, plant surface soil is eluted with water and is rinsed 15 minutes with flowing water again.In ultra-clean work
First with the beautiful plant 30 seconds for the alcohol-pickled rainbow that volume fraction is 70% on platform, then with sterile water washing 3 times, every time 2 minutes；
Take the stem section of the jade of rainbow that explant is transferred in the mercuric chloride solution that mass fraction is 0.1% and soaks 3 points as explant
Clock, then with sterile water washing 6 times, 2 minutes every time, obtain aseptic explant；
(2) induction of Multiple Buds
Inoculated after stem section first to be cut into 1cm or so segment in culture medium, every bottle is inoculated with 3 sections, in 26 DEG C, illumination
Intensity 1500lux, light application time be 24 hours/day, cultivate 6 days in the environment of relative humidity 55% after explant take root, continuation
Culture is germinateed after 5 days obtains Multiple Buds；By above-mentioned 11 days Fiber differentiations, the beautiful blade of rainbow induced synthesis Multiple Buds, see
Fig. 2；
(3) subculture：Multiple Buds are accessed in subculture medium and continue to cultivate 2 weeks intact plants taken root；
(4) transplant：It will be rinsed well with the culture medium remained on intact plant root, In Shade to air-dry 1 day, transplanting is extremely
In sand soil, shade, temperature be 22 DEG C, relative humidity be 55% time grow.
Embodiment 3
In the present embodiment：It is using MS culture mediums as minimal medium, in every liter of minimal medium to induce Multiple Buds culture medium
1.5mg 6-benzyladenines, 1.5mg heteroauxins, 30g sucrose, 8.4g agar are added, 7g activated carbons are formulated, then are used
NaOH regulation pH value is 6.0；
Subculture medium is using MS culture mediums as minimal medium, and 1.0mg6- benzyls are added into every liter of minimal medium
Adenine, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, 5g activated carbons, then it is 5.82 to adjust pH value with NaOH.
(1) selection and sterilization of explant
The beautiful plant of healthy rainbow is chosen, plant surface soil is eluted with water and is rinsed 15 minutes with flowing water again.In ultra-clean work
First with the beautiful plant 30 seconds for the alcohol-pickled rainbow that volume fraction is 70% on platform, then with sterile water washing 3 times, every time 2 minutes；
Take the blade of the jade of rainbow that explant is transferred in the mercuric chloride solution that mass fraction is 0.1% and soaks 10 points as explant
Clock, then with sterile water washing 6 times, 2 minutes every time, obtain aseptic explant；
(2) induction of Multiple Buds
Aseptic explant is inoculated in the induction Multiple Buds culture medium in tissue culture bottle, every bottle is inoculated with 4, in 24 DEG C, light
According to intensity 1000lux, light application time be 24 hours/day, cultivate 6 days in the environment of relative humidity 65% after explant take root, after
Continuous culture is germinateed after 5 days obtains Multiple Buds；By above-mentioned 11 days Fiber differentiations, the beautiful blade of rainbow induced synthesis Multiple Buds；
(3) subculture：Multiple Buds are accessed in subculture medium and continue to cultivate 2 weeks intact plants taken root；
(4) transplant：It will be rinsed well with the culture medium remained on intact plant root, In Shade to air-dry 1 day, transplanting is extremely
In sand soil, shade, temperature be 22 DEG C, relative humidity be 65% time grow.
Comparative example
In comparative example：Induction Multiple Buds culture medium is using MS culture mediums as minimal medium, is added in every liter of minimal medium
Enter 1.5mg 6-benzyladenines, 1.5mg heteroauxins, 30g sucrose, 8.4g agar to be formulated, then pH value is adjusted with NaOH
For 6.0；
Subculture medium is using MS culture mediums as minimal medium, and 1.0mg6- benzyls are added into every liter of minimal medium
Adenine, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, then it is 5.82 to adjust pH value with NaOH.
For comparative example using the blade of the jade of rainbow as explant, cultural method, condition are identical with embodiment 1.In Multiple Buds
Induction Process in, do not take root when cultivating to 6 days, also do not germinate, cultivate to 10 days partial blades and brown stain occurs, see Fig. 4-
5.And the culture medium of activated carbon is with the addition of using the present invention, induction and squamous subculture through Multiple Buds grow intact plant, see figure
1、3.In summary, the induction Multiple Buds culture medium in the present invention and subculture medium with the addition of the charcoal absorption of suitable concentration
Harmful substance, the brown stain to explant have certain inhibitory action, and then improve the breeding coefficient of the jade of rainbow, breeding speed
Degree and survival rate of plant, improve the economic benefit of grower.
The specific embodiment of the invention and application effect are explained in detail above, but the present invention be not limited to it is above-mentioned
Embodiment, can also be without departing from the inventive concept of the premise in those skilled in the art's possessed knowledge
Various changes can be made.
Claims (2)
1. a kind of tissue rapid propagation method of the jade of succulent rainbow, it is characterised in that this method comprises the following steps：
(1) selection and sterilization of explant：The jade of the clean rainbow of surface clean is used to 70vt%-75vt% alcohol-pickled 20-
30 seconds, it was explant to take the beautiful blade of rainbow or stem section, and explant is soaked into 3-10 minutes with 0.1wt% mercuric chloride solution,
Aseptic explant is obtained after having soaked every time with sterile water washing；
(2) induction of Multiple Buds：Aseptic explant is inoculated in induction Multiple Buds culture medium, in 23-26 DEG C, intensity of illumination
800-1500lux, light application time be 24 hours/day, cultivate 6-12 days in the environment of relative humidity 55%-65% after grown thickly
Bud；Induction Multiple Buds culture medium is using MS culture mediums as minimal medium, and 1.0mg6- benzyls are added into every liter of minimal medium
Adenine, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, 5g activated carbons, pH value 5.82；
(3) subculture, transplanting；
Described subculture is：The young shoot for the Multiple Buds that step (2) culture is obtained, which is cut, to be transferred in subculture medium, cultivates 2-
It can obtain intact plant within 3 weeks；Described subculture medium is using MS culture mediums as minimal medium, to every liter of minimal medium
Middle addition 1.0mg 6-benzyladenines, 1.0mg heteroauxins, 30g sucrose, 8.0g agar, 5g activated carbons, pH value 5.82.
2. the tissue rapid propagation method of the jade of succulent rainbow as claimed in claim 1, it is characterised in that described transplanting
For：The culture medium remained on the intact plant obtained through squamous subculture its root is rinsed well, it is In Shade air-dry after transplant
Into sand soil, shade, grown in the case where temperature is 18-26 DEG C, relative humidity is 55%-65%.
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| CN106577294B (en) * | 2016-12-24 | 2018-08-24 | 河南中医药大学 | A kind of method for in-vitro rapid propagation of Ji Yu dew |
| CN107182487A (en) * | 2017-05-31 | 2017-09-22 | 柳州市盛顺种植场 | A kind of beautiful butterfly breeding method |
| CN108719065A (en) * | 2018-05-21 | 2018-11-02 | 句容市茂润苗木有限公司 | A kind of succulent rapid propagation method |
| CN111066654A (en) * | 2019-12-11 | 2020-04-28 | 云南爱花多肉花卉有限公司 | Tissue culture rapid propagation method of succulent plants |
| CN112997881A (en) * | 2021-02-02 | 2021-06-22 | 中信建设有限责任公司 | Sedum aizoon explant sterilization method, callus induction culture medium and induction method |
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| CN101444185A (en) * | 2006-12-15 | 2009-06-03 | 浙江大学 | Method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit |
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| CN101444185A (en) * | 2006-12-15 | 2009-06-03 | 浙江大学 | Method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit |
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