CN113925058B - Mixed acid treatment solution for removing green algae fixed on Porphyra yezoensis cultivation raft frame and method for removing green algae - Google Patents
Mixed acid treatment solution for removing green algae fixed on Porphyra yezoensis cultivation raft frame and method for removing green algae Download PDFInfo
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- CN113925058B CN113925058B CN202111282288.2A CN202111282288A CN113925058B CN 113925058 B CN113925058 B CN 113925058B CN 202111282288 A CN202111282288 A CN 202111282288A CN 113925058 B CN113925058 B CN 113925058B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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Abstract
The invention provides a mixed acid treatment solution for removing green algae fixed on a Porphyra yezoensis cultivation raft frame and a method for removing the green algae, wherein the solvent of the mixed acid treatment solution is sea water in a Porphyra yezoensis cultivation area, and the solute of the mixed acid treatment solution comprises 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate; the pH value of the mixed acid treatment solution is 2.0-2.4, wherein the volume ratio of hydrochloric acid to citric acid solution is 4:3; the mixed acid treatment liquid is utilized, and more than 90% of green algae attached to the cable of the laver raft frame and the net curtain can be killed after 1min; the mixed acid treatment liquid has no obvious influence on the growth of the Porphyra yezoensis in the operation process, and the Porphyra yezoensis can quickly recover the growth state in a short time when the fixed green algae are removed, so that the invention can further promote the benign development of the Porphyra yezoensis breeding industry.
Description
Technical Field
The invention belongs to the technical field of removing fouling organisms in a Porphyra yezoensis cultivation process, and particularly relates to an environment-friendly mixed acid treatment solution for removing green algae fixed on a Porphyra yezoensis cultivation raft frame and a method for removing the green algae.
Background
The Porphyra yezoensis cultivation economic benefit is obvious, and the seaweed is also a health food and is deeply favored by people. Porphyra yezoensis is a main laver cultivation species in Jiangsu province and Shandong province in China at present, and the cultivation scale is huge. Along with the increase of global warming, the laver cultivation industry in the northern hemisphere gradually expands to the area with latitude far north, and the Liaoning province has also tried to develop Porphyra yezoensis cultivation.
The Porphyra yezoensis cultivation facility mainly comprises a net curtain, a cable, a bamboo pole, a bracket, a foam pontoon and the like, and has the advantages of simple structure, time and labor saving in assembly.
However, in the Porphyra yezoensis cultivation process, fouling organisms such as Ulva (blidinia minima), enteromorpha enteromorpha (Ulva intestinalis), enteromorpha prolifera (Ulva prolifera), enteromorpha prolifera (Ulva clathrate), enteromorpha limnodosa (Ulva linza), qu Hutai (Ulva flexuosa), enteromorpha platyphylla (Ulva combssa), eriosema chinense (Tetraclita squamosa) and the like are easily fixed and grown on the cultivation raft frame. The laver raft frame fouling organisms have a periodical species succession process, so that the laver raft frame fouling organisms have rich biodiversity, the presence of the fouling organisms can influence the growth of the laver, the laver raft frame fouling organisms occupy ecological niches of the laver, and finally the yield of the laver and the income level of laver farmers are influenced.
Research finds that the laver raft frame fouling organisms mainly grow on cables and net curtains, and the time and the labor are wasted for removing the fouling organisms, and in the treatment process, the growth of the porphyra yezoensis on the net curtains needs to be prevented from being influenced, so that no effective mode is provided for treating the raft frame fouling organisms.
In addition, if the fouling organisms of the laver raft frames are not treated, the enteromorpha fallen off by the laver culture raft frames is extremely easy to cause outbreak of yellow sea Green tide (Green tide), and the method has great influence on the offshore area and the intertidal zone ecosystem of the coastal city of south yellow sea in China. It is therefore of great importance to develop a method for removing cable and screen sessile biofouling organisms.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame and a method for removing the green algae by using the mixed acid treatment solution.
To achieve the above object, the solution of the present invention is:
the invention provides a mixed acid treatment solution for removing green algae fixed on a Porphyra yezoensis cultivation raft frame, wherein the solvent of the mixed acid treatment solution is sea water in a Porphyra yezoensis cultivation area, and the solute of the mixed acid treatment solution comprises 0.1-0.2wt% of standard hydrochloric acid (38%), 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3.
The second object of the invention is to provide a method for removing fixed green algae of a porphyra yezoensis cultivation raft frame curtain by using the mixed acid treatment solution, which comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3;
(2) Removing the net curtain of the cultured Porphyra yezoensis during the period of damping off, and immersing the net curtain in the mixed acid treatment solution for 1min; and then taking out the net curtain for cultivating the Porphyra yezoensis, immersing the net curtain into clean seawater, and fully rinsing until no residual solution exists on the net curtain for cultivating the Porphyra yezoensis.
Preferably, in the step (2), when the curtain of the thallus Porphyrae is immersed in the mixed acid treatment liquid, the pH value of the mixed acid treatment liquid is monitored by using a portable pH meter, and if the pH value is higher than 2.4, the mixed acid treatment liquid needs to be timely supplemented.
Preferably, the laver recovery period: the treated net curtain for cultivating Porphyra yezoensis needs to be cultivated in the sea area for 7 days so as to carry out nutrition transformation and leaf surface restoration, and the Porphyra yezoensis can be harvested.
The invention provides a method for removing cable fixation green algae on a porphyra yezoensis cultivation raft frame by using the mixed acid treatment solution, which comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3;
(2) Loading the mixed acid treatment liquid into a spraying device (such as a backpack pesticide spraying machine), spraying the mixed acid treatment liquid onto a cable on a Porphyra yezoensis cultivation raft frame, reloading the recovered mixed acid treatment liquid into the spraying device, and timely monitoring the pH value of the mixed acid treatment liquid, wherein if the pH value is higher than 2.4, the mixed acid treatment liquid needs to be timely replenished.
By adopting the scheme, the invention has the beneficial effects that:
according to the invention, the mixed acid treatment liquid is utilized to treat for 1min, more than 90% of green algae attached to the cable of the laver raft frame and the net curtain can be killed, so that the effect of removing the green algae is achieved; the mixed acid treatment liquid has no obvious influence (or little influence) on the growth of the Porphyra yezoensis in the operation process, and the Porphyra yezoensis can also quickly recover the growth state in a short time while the fixed green algae are removed. Therefore, the invention can better ensure the growth of Porphyra yezoensis and further promote the benign development of the Porphyra yezoensis breeding industry.
Drawings
FIG. 1 is a schematic view of green algae attached to a laver net according to the present invention.
FIG. 2 is a schematic diagram of the present invention, in which a laver net is soaked in seawater to clean the surface of the laver net with residual acid.
FIG. 3 is a schematic view of a screen not treated with a mixed acid treatment of the present invention.
FIG. 4 is a schematic view of a screen treated with a mixed acid treatment of the present invention.
Fig. 5 is a schematic view of green algae attached to a laver rope according to the present invention.
FIG. 6 is a schematic representation of a cable of the present invention untreated with a mixed acid treatment solution.
FIG. 7 is a schematic representation of a cable treated with a mixed acid treatment of the present invention.
FIG. 8 is a data schematic of photosynthetic fluorescence parameters of Enteromorpha prolifera according to the present invention.
FIG. 9 is a data diagram of photosynthetic fluorescence parameters of Porphyra yezoensis according to the present invention.
Detailed Description
The invention provides a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame and a method for removing the green algae.
< Mixed acid treatment solution for removing Chlorella fixed on Porphyra yezoensis cultivation raft frame >)
The solvent in the environment-friendly mixed acid treatment solution for removing the green algae fixed on the Porphyra yezoensis cultivation raft frame is the seawater in the local Porphyra yezoensis cultivation area, and the solute of the mixed acid treatment solution comprises 0.1-0.2wt% of standard hydrochloric acid (38%), 50-60g/L anhydrous citric acid, 0.04-0.06g/L sodium glycerophosphate and 0.1-0.3mg/L disodium ethylenediamine tetraacetate; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3.
In fact, sodium glycerophosphate and disodium ethylenediamine tetraacetate contribute to restoration of the sea area of Porphyra yezoensis.
< method for removing fixed green algae of Porphyra yezoensis culture raft frame curtain by using mixed acid treatment solution >)
As shown in fig. 1 to 4, the method for removing fixed green algae of a porphyra yezoensis culture raft curtain by using a mixed acid treatment solution of the invention comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3;
(2) Removing the net curtain of the cultured Porphyra yezoensis during the period of damping off, and immersing the net curtain in the mixed acid treatment solution for 1min; and then taking out the net curtain for cultivating the Porphyra yezoensis, immersing the net curtain into clean seawater, and fully rinsing until no residual solution exists on the net curtain for cultivating the Porphyra yezoensis.
Preferably, in the step (2), when the curtain of the porphyra yezoensis is immersed in the mixed acid treatment liquid, the pH value of the mixed acid treatment liquid is monitored by using a portable pH meter, and if the pH value is higher than 2.4, the mixed acid treatment liquid needs to be timely supplemented.
Preferably, the laver recovery period: the treated net curtain for cultivating Porphyra yezoensis needs to be cultivated in the sea area for 7 days so as to carry out nutrition transformation and leaf surface restoration, and the Porphyra yezoensis can be harvested.
< method for removing Cable-fixed green algae on Porphyra yezoensis culture raft frame by using mixed acid treatment solution >)
As shown in fig. 5 to 7, the method for removing cable-fixed green algae on a porphyra yezoensis cultivation raft frame by using a mixed acid treatment solution of the invention comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3;
(2) Loading the mixed acid treatment liquid into a spraying device (such as a backpack pesticide spraying machine), spraying the mixed acid treatment liquid onto a cable on a Porphyra yezoensis cultivation raft frame, reloading the recovered mixed acid treatment liquid into the spraying device, and timely monitoring the pH value of the mixed acid treatment liquid, wherein if the pH value is higher than 2.4, the mixed acid treatment liquid needs to be timely supplemented.
The technical contents of the present invention are further described below with reference to examples. The following examples are illustrative, not limiting, and are not intended to limit the scope of the invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Examples:
the method for removing the fixed green algae of the porphyra yezoensis cultivation raft frame curtain by using the mixed acid treatment liquid comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1wt% of standard hydrochloric acid, 50g/L of anhydrous citric acid, 0.04g/L of sodium glycerophosphate and 0.1mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain an environment-friendly mixed acid treatment solution for removing green algae fixed on a laver cultivation raft frame; the pH value of the mixed acid treatment solution is 2.2, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3.
(2) And during the period of damping, taking down the curtain of the cultured Porphyra yezoensis, immersing the curtain in the mixed acid treatment solution, fully immersing the curtain for 1min, taking out the curtain of the cultured Porphyra yezoensis, immersing the curtain in clean seawater, and fully rinsing until no solution remains on the curtain of the cultured Porphyra yezoensis.
In the treatment process, a portable pH meter is used for monitoring the pH value of the mixed acid treatment liquid, and if the pH value is higher than 2.4, the mixed acid treatment liquid needs to be timely replenished.
(3) Loading the mixed acid treatment liquid obtained in the step (1) into a backpack pesticide spraying machine, spraying the mixed acid treatment liquid onto a cable on a porphyra yezoensis cultivation raft frame, reloading the recovered mixed acid treatment liquid into the backpack pesticide spraying machine, monitoring the pH value of the mixed acid treatment liquid in time, and if the pH value is higher than 2.4, supplementing the mixed acid treatment liquid in time.
(4) Laver recovery period: the processed Porphyra yezoensis net curtain can be harvested after 7 days of sea area cultivation so that Porphyra yezoensis is subjected to nutrition transformation and leaf surface restoration.
< experiment >
And carrying out the following experiment of enteromorpha and laver on the mixed acid treatment solution.
As shown in fig. 8, the group 1 is untreated, the group 2 is hydrochloric acid and citric acid solution with a volume ratio of 4:1, and enteromorpha is not completely killed after treatment and recovery; the volume ratio of the group 3 to the hydrochloric acid to the citric acid solution is 4:3, and the enteromorpha is completely dead after treatment and recovery; the volume ratio of the hydrochloric acid to the citric acid solution in the group 4 is 4:5, and the enteromorpha is completely killed after treatment and recovery. And comparing the ratio of the hydrochloric acid to the citric acid through different groups of treatment and recovery conditions, and selecting a group with the optimal treatment effect, namely, the volume ratio of the hydrochloric acid to the citric acid solution is 4:3.
As shown in fig. 9, group 1 is untreated; the volume ratio of the group 2 to the hydrochloric acid to the citric acid solution is 4:1, and after treatment and recovery, the laver recovers the health of algae; the volume ratio of the group 3 to the hydrochloric acid to the citric acid solution is 4:3, and after treatment and recovery, the laver recovers the health of algae; the volume ratio of the group 4 to the hydrochloric acid to the citric acid solution is 4:5, and after treatment and recovery, the laver does not completely recover the health of algae.
In summary, the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is preferably 4:3.
As shown in fig. 4 to 9, after the mixed acid treatment solution treatment, the growth state, photosynthetic fluorescence parameters, and biomass of the porphyra yezoensis were not affected.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments. Those skilled in the art will appreciate that, in light of the principles of the present invention, improvements and modifications can be made without departing from the scope of the invention.
Claims (2)
1. A method for removing fixed green algae of Porphyra yezoensis cultivation raft frame net curtain by mixed acid treatment solution is characterized in that: the method comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3;
(2) Removing the net curtain of the cultured Porphyra yezoensis during the period of damping off, and immersing the Porphyra yezoensis in the mixed acid treatment solution for 1min; then taking out the net curtain for cultivating the Porphyra yezoensis, immersing the net curtain into clean seawater, and fully rinsing until no residual solution exists on the net curtain for cultivating the Porphyra yezoensis;
in the step (2), when the curtain of the cultured Porphyra yezoensis is immersed in the mixed acid treatment liquid, the pH value of the mixed acid treatment liquid is monitored in time, and if the pH value is higher than 2.4, the mixed acid treatment liquid needs to be supplemented in time;
the treated net curtain for cultivating Porphyra yezoensis needs to be cultivated in the sea area for 7 days so as to carry out nutrition transformation and leaf surface restoration, and the Porphyra yezoensis can be harvested.
2. The method for removing cable fixation green algae on a porphyra yezoensis cultivation raft frame by using the mixed acid treatment solution according to claim 1, which is characterized in that: the method comprises the following steps:
(1) Preparing a mixed acid treatment solution for removing green algae fixed on the porphyra yezoensis cultivation raft frame: dissolving 0.1-0.2wt% of standard hydrochloric acid, 50-60g/L of anhydrous citric acid, 0.04-0.06g/L of sodium glycerophosphate and 0.1-0.3mg/L of disodium ethylenediamine tetraacetate into sea water in a laver cultivation area to obtain a mixed acid treatment solution for removing green algae fixed on a porphyra yezoensis cultivation raft frame; the pH value of the mixed acid treatment solution is 2.0-2.4, and the volume ratio of hydrochloric acid to citric acid solution in the mixed acid treatment solution is 4:3;
(2) And loading the mixed acid treatment liquid into a spraying device, spraying the mixed acid treatment liquid onto a cable on a porphyra yezoensis cultivation raft frame, reloading the recovered mixed acid treatment liquid into the spraying device, monitoring the pH value of the mixed acid treatment liquid in time, and if the pH value is higher than 2.4, supplementing the mixed acid treatment liquid in time.
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| CN113925058A (en) | 2022-01-14 |
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