CN111528082A - Method for removing attached foreign algae in cultivation process of sargassum fusiforme - Google Patents
Method for removing attached foreign algae in cultivation process of sargassum fusiforme Download PDFInfo
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Abstract
The invention discloses a method for removing attached miscellaneous algae in a sargassum fusiforme culture process, which comprises the steps of soaking sargassum fusiforme larvae attached by green algae in an acid solution for a period of time, transferring the sargassum fusiforme larvae into filtered seawater added with phytohormone for continuous culture, and dying the green algae after a period of time, wherein the sargassum fusiforme larvae are basically not influenced and can quickly recover normal growth. The method is simple to operate, saves time, is effective, and can solve the key problem of the attachment of the miscellaneous algae in the cultivation of the sargassum fusiforme.
Description
Technical Field
The invention relates to a method for removing miscellaneous algae and rapidly restoring normal growth of sargassum fusiforme larvae by using hormone in a sargassum fusiforme cultivation process.
Background
The sargassum fusiforme is a natural marine algae food, contains abundant polysaccharide, edible fiber, vitamins and various trace elements, has higher nutritional and medicinal values, and is a traditional food loved by people in China, Japan and Korea. Zhejiang province and Shantou county are the most important cultivation and processing bases for Cyrtymenia Sparsa in China, and the cultivation area, the yield and the export number of the Cyrtymenia Sparsa are the first places, so that the method is known as 'hometown of Cyrtymenia Sparsa in China'. The propagation mode of the sargassum fusiforme is mainly divided into sexual propagation and asexual propagation, wherein the sexual propagation refers to that sperms and ova are combined to generate sporophytes and then grow into seedlings; vegetative propagation refers to the growth of seedlings into adult plants through rhizoid regeneration. At present, the cultivation of offshore sargassum fusiforme in China is mainly carried out by sexual propagation and seedling.
In the cultivation period of the sargassum fusiforme in the sea area, especially from early spring to early summer each year, the cultivation period is not only the centralized period of sargassum fusiforme seedling culture, but also the most luxuriant season of marine organisms such as various seaweeds. In research, it has been found that during the cultivation period of the sea area of the economic algae such as sargassum fusiforme, especially during the summer crossing of the seedling curtain, a great amount of macroalgae such as Ulva (Ulva) are attached to the cultivation raft frame and the net curtain, even directly attached to the cultivation algae. The attached algae and other organisms compete with the economic algae Hizikia fusiforme for inorganic carbon, nutrient substances, illumination, space (net curtain or raft frame) and other environmental resources. And the fast growth of the epiphytic algae interferes the physiological processes of the sargassum fusiforme, such as photosynthesis and the like, influences the growth, development and morphogenesis of the sargassum fusiforme, particularly sargassum fusiforme larvae, finally reduces the yield and quality of the sargassum fusiforme, and seriously influences the cultivation of the economic sargassum fusiforme.
At present, methods for removing the miscellaneous algae from the sargassum fusiforme mainly comprise a manual picking method, a chemical reagent treatment and the like. The manual picking method is time-consuming and labor-consuming, the removal effect of the mixed algae is poor, only the surface mixed algae can be removed because part of the mixed algae is attached to the inner part of the rhizoid of the sargassum fusiforme, the complete removal effect is difficult to achieve, and the mixed algae can still be regenerated after a period of time. The chemical agent treatment comprises treating the sargassum fusiforme with 8mmol/L sodium hypochlorite for 18min or using 5-10% ammonium sulfate seawater to perform medicated bath on the sargassum fusiforme for 5-10min to remove attached miscellaneous algae, and the like, which can not completely and effectively remove the attached miscellaneous algae of the cultivated sargassum fusiforme, and the treated sargassum fusiforme can completely recover the normal growth after a long time. Therefore, an appropriate method is found to solve the problem of green algae adhesion in the cultivation process of the sargassum fusiforme, and the method has important practical significance for artificial cultivation of the sargassum fusiforme.
Disclosure of Invention
The invention aims to provide a method for removing attachment of green algae in the growth process of sargassum fusiforme larvae and quickly recovering the sargassum fusiforme larvae after treatment.
In order to achieve the purpose, the invention adopts the technical scheme that the method for removing the attached mixed algae in the cultivation process of the sargassum fusiforme is characterized by comprising the following steps:
the method comprises the following steps: preparing 8% citric acid bath solution with deionized water, and introducing into a medicinal bath pool for disinfection and standing in advance;
step two: transferring the young sargassum fusiforme attached with the miscellaneous algae (mainly green algae) from the first culture pond to a medicinal bath pond, completely soaking the young sargassum fusiforme in 8% citric acid medicinal bath liquid for 3min at the temperature of 20-23 ℃, and starting water circulation of the medicinal bath pond to keep the liquid in the medicinal bath pond flowing automatically;
step three: transferring the sargassum fusiforme larvae soaked for 3min to a cleaning pool, starting water circulation of the cleaning pool, and washing the sargassum fusiforme larvae clean by using prefiltered and disinfected primary seawater for 30-60 s;
step four: transferring the cleaned sargassum fusiforme larvae in the third step to a second culture tank, wherein primary seawater containing 1.5mg/L naphthylacetic acid (NAA) and subjected to pre-filtration and disinfection is prepared in the second culture tank, and sufficient dissolved oxygen is ensured to be contained in the water, so that the illumination intensity of 90-120 mu mol/m/s is ensured;
step five: the recovery culture of the step four lasts for 9-10 h, the fact that the juvenile sargassum fusiforme in the pool is recovered to a normal growth state can be directly observed under specific conditions, and the comparison between the surface color and texture softness of the juvenile sargassum fusiforme and the normal juvenile sargassum fusiforme is mainly observed;
step six: after the larvae of the sargassum fusiforme are restored to a normal growth state, the larvae are transferred to the first culture pond with the culture solution replaced to continue to restore the normal culture process.
In the method, the dipping time of the second step is strictly controlled to be 3min with an error of less than 10 s.
In the method, the three washing steps are to wash the sargassum fusiforme larvae comprehensively at multiple angles, so that the situation that the recovery is influenced by bringing the medicinal bath liquid into the second culture pond due to the fact that the washing is not in place is avoided.
In the method of the present invention, the texture and color of the young hijiki in step five after the recovery culture are comparable to those of normal young hijiki, and if the texture is found to be too soft or the color is found to be too light, the recovery culture time in the second culture tank can be increased, but it is not preferable to exceed 10 hours.
The method utilizes the citric acid to soak the larvae of the sargassum fusiforme attached by the mixed algae, can effectively remove the green algae, has very simple operation, easy operation in practical application and short treatment time, and saves time compared with the traditional physical method for removing the mixed algae. The juvenile Sargassum fusiforme soaked by citric acid is treated by hormone (NAA) to recover rapidly, and compared with natural recovery in seawater, the juvenile Sargassum fusiforme has good recovery effect and good growth state. The method can solve the problems that the Sargassum fusiforme is polluted by the mixed algae in the laboratory culture and the Sargassum fusiforme seedlings are adhered by the mixed algae in the actual culture process.
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FIG. 1 is a graph showing comparative experimental records before and after the treatment of Hizikia fusiforme larvae with attached green algae with citric acid;
FIG. 2 is a graph showing comparative experimental records of the removal of green algae and the status of Hizikia fusiforme larvae after soaking the larvae with the green algae in 8% citric acid for 3min,3h, 6h and 9h, respectively;
FIG. 3 is a graph showing a record of the growth process of the young Sargassum fusiforme which is recovered and cultured by using filtered seawater to which 1.5mg/L of naphthylacetic acid (NAA) is added after the young Sargassum fusiforme which is attached with green algae is treated with acid;
FIG. 4 is a graph showing the comparison between the growth of the larvae of Hizikia fusiforme using filtered seawater recovery culture with 1.5mg/L naphthylacetic acid (NAA) added and natural filtered seawater recovery culture.
Detailed Description
The following examples of the present invention are described in detail, and the examples are intended to illustrate the present invention and should not be construed as limiting the present invention, and those without specifying any particular technique or condition in the examples are performed according to the techniques or conditions described in the literature in the art or according to the product specification, and all reagents or apparatuses are not specified, and are all conventional products commercially available.
As shown in fig. 1 to 4, an embodiment of the present invention is specifically a method for removing attached miscellaneous algae in a sargassum fusiforme culture process, which is characterized by comprising the following steps:
the method comprises the following steps: preparing 8% citric acid bath solution with deionized water, and introducing into a medicinal bath pool for disinfection and standing in advance;
step two: transferring the young sargassum fusiforme attached with the miscellaneous algae (mainly green algae) from the first culture pond to a medicinal bath pond, completely soaking the young sargassum fusiforme in 8% citric acid medicinal bath liquid for 3min at the temperature of 20-23 ℃, and starting water circulation of the medicinal bath pond to keep the liquid in the medicinal bath pond flowing automatically;
step three: transferring the sargassum fusiforme larvae soaked for 3min to a cleaning pool, starting water circulation of the cleaning pool, and washing the sargassum fusiforme larvae clean by using prefiltered and disinfected primary seawater for 30-60 s;
step four: transferring the cleaned sargassum fusiforme larvae in the third step to a second culture tank, wherein primary seawater containing 1.5mg/L naphthylacetic acid (NAA) and subjected to pre-filtration and disinfection is prepared in the second culture tank, and sufficient dissolved oxygen is ensured to be contained in the water, so that the illumination intensity of 90-120 mu mol/m/s is ensured;
step five: the recovery culture of the step four lasts for 9-10 h, the fact that the juvenile sargassum fusiforme in the pool is recovered to a normal growth state can be directly observed under specific conditions, and the comparison between the surface color and texture softness of the juvenile sargassum fusiforme and the normal juvenile sargassum fusiforme is mainly observed;
step six: after the larvae of the sargassum fusiforme are restored to a normal growth state, the larvae are transferred to the first culture pond with the culture solution replaced to continue to restore the normal culture process.
In the method, the dipping time of the second step is strictly controlled to be 3min with an error of less than 10 s.
In the method, the three washing steps are to wash the sargassum fusiforme larvae comprehensively at multiple angles, so that the situation that the recovery is influenced by bringing the medicinal bath liquid into the second culture pond due to the fact that the washing is not in place is avoided.
In the method of the present invention, the texture and color of the young hijiki in step five after the recovery culture are comparable to those of normal young hijiki, and if the texture is found to be too soft or the color is found to be too light, the recovery culture time in the second culture tank can be increased, but it is not preferable to exceed 10 hours.
Example 1 comparative experiment of Hizikia fusiforme larvae with attached foreign algae treated with 8% citric acid, the procedure was as follows:
1) deionized water was used to formulate 8% citric acid.
2) Soaking the Sargassum fusiforme larva with attached Sargassum fusiforme in 8% citric acid solution, and recording the soaking time for 3min by using a timer. (as shown in figure 1, A-C is Sargassum fusiforme larva attached with green algae before soaking, D-E is Sargassum fusiforme larva treated with citric acid)
3) Taking out the larvae of the sargassum fusiforme, washing the larvae with naturally filtered seawater for 40s, and dividing the larvae into two groups of comparison experiments:
experimental groups: putting the sargassum fusiforme larvae into filtered seawater added with 1.5mg/L of naphthylacetic acid (NAA) for continuous culture;
comparison group: putting the sargassum fusiforme larvae into naturally filtered seawater for recovery culture.
4) The method ensures that the water contains sufficient dissolved oxygen, ensures the illumination intensity of 90-120 mu mol/m/s, and observes and records two groups of experimental sargassum fusiforme seedling states which are carried out synchronously every three hours:
after 3h, the green algae are observed not to fade;
observing after 6h, soaking for 3min with 8% citric acid, wherein the green algae death rate is 66.7%, and the young Cyrtymenia Sparsa slightly fades;
after 9h, the larvae are soaked by 8% citric acid for 3min, and green algae death conditions of the two groups are basically the same. (as shown in figure 2, A is sargassum fusiforme larva attached by green algae; B is sargassum fusiforme larva treated with 8% citric acid for 3min and observed after 3H; C is sargassum fusiforme larva treated with 8% citric acid for 3min and observed after 6H; D is sargassum fusiforme larva treated with 8% citric acid for 3min and observed after 9H; E-H is local detail corresponding to A-D)
5) The recovery condition of the sargassum fusiforme larvae after green algae removal:
experimental groups: the young Hizikia fusiforme which is recovered and cultured by using seawater added with naphthylacetic acid (NAA) basically recovers to a normal growth state and is hard.
Comparison group: the color and luster of the larvae which are recovered and cultured by using the natural seawater filtration are still light, and the tested texture is found to be softer than that of the normal sargassum fusiforme larvae. (as shown in figure 4, A-D is the seawater added with NAA hormone after being treated with 8% citric acid, and E-H is the seawater added with NAA hormone after being treated with 8% citric acid and cultured)
6) According to the results, the 8% citric acid can completely remove the green algae attached to the sargassum fusiforme larvae after 3min treatment for 9h, and the death rate of the green algae reaches 100%.
7) As can be seen from the above, the larvae of Cyrtymenia Sparsa can be restored to a normal growth state more rapidly by the seawater restoration culture containing 1.5mg/L of naphthylacetic acid (NAA) than by the natural seawater restoration culture.
The method utilizes the citric acid to soak the larvae of the sargassum fusiforme attached by the mixed algae, can effectively remove the green algae, has very simple operation, easy operation in practical application and short treatment time, and saves time compared with the traditional physical method for removing the mixed algae. And then treating the larvae of the sargassum fusiforme soaked by the citric acid by using hormone (NAA) to quickly recover the larvae of the sargassum fusiforme, and compared with natural recovery by placing the larvae in seawater, the larvae of the sargassum fusiforme have good recovery effect and better later-stage growth condition. The method can solve the problems that the Sargassum fusiforme is polluted by the mixed algae in the laboratory culture and the Sargassum fusiforme seedlings are adhered by the mixed algae in the actual culture process.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and it is to be understood that the above description is not to be construed as limiting the present invention, and any modifications, equivalents, improvements and the like made within the design concept of the present invention are included in the scope of the present invention.
Claims (4)
1. A method for removing attached hybrid algae in the cultivation process of sargassum fusiforme is characterized by comprising the following steps:
the method comprises the following steps: preparing 8% citric acid bath solution with deionized water, and introducing into a medicinal bath pool for disinfection and standing in advance;
step two: transferring the young sargassum fusiforme attached with the miscellaneous algae (mainly green algae) from the first culture pond to a medicinal bath pond, completely soaking the young sargassum fusiforme in 8% citric acid medicinal bath liquid for 3min at the temperature of 20-23 ℃, and starting water circulation of the medicinal bath pond to keep the liquid in the medicinal bath pond flowing automatically;
step three: transferring the sargassum fusiforme larvae soaked for 3min to a cleaning pool, starting water circulation of the cleaning pool, and washing the sargassum fusiforme larvae clean by using prefiltered and disinfected primary seawater for 30-60 s;
step four: transferring the cleaned sargassum fusiforme larvae obtained in the third step to a second culture recovery pond containing 1.5mg/L naphthylacetic acid (NAA) of prefiltered and disinfected primary seawater, ensuring that the water contains sufficient dissolved oxygen, ensuring the illumination intensity of 90-120 mu mol/m/s and ensuring manual control 12: 12 to 15: 9 light period;
step five: the recovery culture of the step four lasts for 9-10 h, the fact that the juvenile sargassum fusiforme in the pool is recovered to a normal growth state can be directly observed under specific conditions, and the comparison between the surface color and texture softness of the juvenile sargassum fusiforme and the normal juvenile sargassum fusiforme is mainly observed;
step six: after the larvae of the sargassum fusiforme are restored to a normal growth state, the larvae are transferred to the first culture pond with the culture solution replaced to continue to restore the normal culture process.
2. The method for removing the attached miscellaneous algae during the cultivation of sargassum fusiforme according to claim 1, wherein the method comprises the following steps: and the medicated bath time in the second step is strictly controlled to be 3min with an error of less than 10 s.
3. The method for removing the attached miscellaneous algae during the cultivation of sargassum fusiforme according to claim 2, wherein the method comprises the following steps: and in the third washing step, the sargassum fusiforme larvae are washed comprehensively at multiple angles, so that the situation that the recovery is influenced by bringing the liquid medicine into the second culture pond due to the fact that the liquid medicine is not washed in place is avoided.
4. The method of claim 3, wherein the method comprises the following steps: the texture and color of the young sargassum fusiforme after recovery culture in the fifth step are the same as those of normal sargassum fusiforme, if the texture is too soft or the color is too light, the recovery culture time in the second culture pond can be properly increased, but the recovery culture time is not longer than 10 hours.
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Cited By (1)
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| CN113973799A (en) * | 2021-10-12 | 2022-01-28 | 广东海洋大学 | Application of hydroxyethyl sulfonic acid in removing adsorption type benthic diatom |
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