CN101263780A - Northern sea area Sargassum thunbergii large size seedling seed breeding method ahead of time - Google Patents
Northern sea area Sargassum thunbergii large size seedling seed breeding method ahead of time Download PDFInfo
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Abstract
The invention provides a method for large scale early bred fingerlings of northern sea area sargassum thunbergii, which is characterized in that, in the middle and last ten days of May, early matured sea surface rafting algae seedlings in south are chosen; the algae seedlings are transported to northern sea area after long-distance transportation; in an indoor seedling workshop, pretreatment and seedling collection are performed to algae seedlings; after the algae seedlings are cultured indoor for about ten days, rafting temporary rearing seedling emergence is carried out in sea, meanwhile standardized sea management is performed; after the marine cultivation of about two months, more than 4cm large sized robust sargassum thunbergii seedlings are obtained.
Description
Technical field
The present invention relates to the method that the marine alga seed is bred, relate in particular to the method that mouse tail algae large size seedling seed is bred in advance.
Background technology
Mouse tail algae (Sargassum thunbergii) is the coastal common wild species brown alga of China, has important economic value.In China, North gets Liaodong Peninsula, reaching the Lezhou Peninsula in the south all has distribution, and its trace spreads all over provinces such as Liaoning, Shandong, Jiangsu, Zhejiang, Fujian, Guangdong.To the going deep into of its research, some important function of mouse tail algae were found gradually: except occupying in marine ecosystems the critical role, also have many developable potentiality in medicine, health care, aquaculture and chemical engineering industry along with in recent years.
At present, flourish along with sea cucumber, abalone culture industry, mouse tail algae wild resource as sea cucumber abalone high-quality bait is excessively gathered, make its natural resources amount day by day fail, do not satisfy the demand that every profession and trade increases year by year, the algae research worker is being devoted to the artificial breeding work of mouse tail algae.Domestic research about mouse tail algae breed aspect launches recent years, and Chinese scholar has been carried out number of research projects at the sexual reproduction process of mouse tail algae, seedling culture etc.In the northern China sea area; the mouse tail algae mature period approximately began in early July; and delay gradually from south orientation north with the sea area; conventional seedbed system is chosen in mid or late July season usually; after the seedling that conventional method is cultivated is plunged into the commercial sea; on the occasion of high temperature season; culturing various assorted algaes in sea area and invertebrate larva breeds in a large number; cover on the mouse tail algae seedling curtain; make mouse tail algae seedling death by suffocation; cause seedling to come off in a large number, often cause whole death of the artificial seedling of mouse tail algae when serious, therefore; the sexual artificial breeding technique of breeding of the scale of mouse tail algae can not be broken through so far; just occurring after seedling is plunged into the commercial sea and cultivates taking off seedling in a large number, can not form large size seedling seed, is the bottleneck problem of being badly in need of solution in the present mouse tail algae artificial breeding process.
Summary of the invention
The method that northern sea area Sargassum thunbergii large size seedling seed of the present invention is bred in advance, it can solve problems such as high temperature season mouse tail algae artificial breeding survival rate that prior art exists is low.
In order to reach the purpose that solves the problems of the technologies described above, technical scheme of the present invention is, the method that a kind of northern sea area Sargassum thunbergii large size seedling seed is bred in advance is characterized in that described method undertaken by following step:
What 1) mouse tail algae planted vegetables choosing and transporting: at southern area, utilize the microscopy receptacle to determine the frond maturity, before the maturation Sheng phase arrives, to plant vegetables from the mouse tail algae of sea results and directly pack into the incubator, and keep low temperature with the seawater ice chest, suitably drench seawater, moistening to guarantee that mouse tail algae plants vegetables, then incubator is added a cover and sealed transportation and arrive northern destination;
2) preliminary treatment that plants vegetables of mouse tail algae: the mouse tail algae of transporting back from south plants vegetables, in the seawater pond, clean, choose the mouse tail algae branch that has receptacle, again sandwich the Miao Shengzhong extension and support in top layer, pond, indoor workshop, every pond hangs supports 3 ropes, 8～15kg, and hanging the foster female-male proportion that plants vegetables is 4～8: 1, carrying out the flowing water inflation cultivates, water temperature is controlled at 18～22 ℃, and intensity of illumination is controlled at 3000～20000lx, and the visible receptacle of naked eyes has the ovum of extension phenomenon after 2～3 days.
3) collection of fertilized egg: with ripe mouse tail algae plant vegetables fully clean after, evenly loose in the pond that arranges the seedling curtain in advance in the shop, it is 0.4～0.6kg that each seedling curtain plants vegetables with female mouse tail algae, male planting vegetables in 4～8: 1 ratio is arranged in pairs or groups.In hydrostatic, gather fertilized egg, in this process, constantly stir seawater or stir and plant vegetables, make fertilized egg come off fast and adhere to evenly, fertilized egg was adhered to time remaining 15～24 hours, the microscopy slide, fertilized egg is adhered to density and is reached 30～40 in every visual field (10 * 10), just can pull out planting vegetables;
4) indoor culture: the seedling curtain after fertilized egg is adhered to, the hydrostatic cultivation changes the miniflow water planting over to after 1～2 day and educates, and changes flowing water after 3～4 days over to and cultivates, water temperature is controlled at 18～22 ℃, intensity of illumination is controlled at 4000～15000lx, and at 12～14 hours sunshine time, nutrient concentration is N0 in the control seawater
3-N:3～6g/m
3, PO
4-P:0.3～0.6g/m
3, the raft frame that floats is set in the pond, make the seedling curtain place the top layer, pond all the time, seedling curtain two ends are exceptionally straight to be strained and fixed on the raft frame, so that seedling is subjected to light even;
The seedling curtain cleans: after collecting seedling 2～3 days, the seedling curtain is scrubbed in beginning swing gently, removes mud scum and assorted algae spore; Bring into use spray gun to scrub the seedling curtain after 5～6 days, seedling curtain two ends are tightened carry out the spary flushing, after 10～15 days indoor culture time, transfer marine raft formula to and cultivate;
5) sea of seedling is cultivated and to be emerged: the setting of a. culture raft frame, between two floating well ropes, one of vertical steel pipe is set every 3～4 meters, and every length is 2～4 meters, tie on floating well rope with rope at the steel pipe two ends, needs 13～18 of steel pipes altogether between per 2 floating well ropes; Form 3～4 * 2～4 meters water surface area between 2 steel pipes;
B. the seedling curtain hangs: seedling curtain two ends lie on the steel pipe with lifting rope, and can put down therebetween and hang chemical fabric seedling curtain 2 * 0.3m is that 2～4 or coir rope seedling curtain 1 * 0.5m are 4～6, the stretching fully flat seawater surface that is hung on;
C. the management of seedling curtain is scrubbed: under the normal condition, the seedling curtain was scrubbed once every 1～2 day, and assurance seedling cleaning is beneficial to carries out light and effect and respiration, promotes that seedling grows fast;
D. regulate seedling curtain water layer: under the normal condition, the seedling curtain is arranged on surface layer of water, when intensity of illumination is 25000lx when above, should be with seedling curtain decline water layer to 0.3～0.5m depth of water place, and marine cultivation the through 50～70 days, seedling reaches the long upright seedling of 4cm.
Choose and delivery period that described mouse tail algae plants vegetables are that mid-May is to late May.
When spray gun was scrubbed the seedling curtain, the ascending scope of hydraulic pressure intensity was 0.3kg/cm
2～1.5kg/cm
2
When scrubbing, adopts indoor culture electrical water jetting formula flusher; The marine cultivation adopted the water jet flusher of diesel powered when scrubbing.
The present invention is that described method is undertaken by following step in the southern area method of cultivating of collecting seedling:
1) collects seedling: at southern area, select local sea manually to press from both sides the seedling raft and support the ripe frond of mouse tail algae, collect seedling as kind of an algae;
2) collection of fertilized egg: with ripe mouse tail algae plant vegetables fully clean after, evenly loose in the pond that arranges the seedling curtain in advance in the shop, it is 0.4～0.6kg that each seedling curtain plants vegetables with female mouse tail algae, male planting vegetables: 1 ratio collocation in 4～8, in hydrostatic, gather fertilized egg, in this process, constantly stir seawater or stir and plant vegetables, make fertilized egg come off fast and adhere to evenly, fertilized egg was adhered to time remaining 15～24 hours, microscopy slide, fertilized egg adhere to density and reach 30～40 in every visual field (10 * 10), just can pull out planting vegetables;
3) indoor culture: the seedling curtain after fertilized egg is adhered to, the hydrostatic cultivation changes the miniflow water planting over to after 1～2 day and educates, and changes flowing water after 3～4 days over to and cultivates, and intensity of illumination is controlled at 4000～15000lx, and at 12～14 hours sunshine time, nutrient concentration is N0 in the control seawater
3-N:3～6g/m
3, PO
4-P:0.3～0.6g/m
3, the raft frame that floats is set in the pond, make the seedling curtain place the top layer, pond all the time, seedling curtain two ends are exceptionally straight to be strained and fixed on the raft frame, so that seedling is subjected to light even;
The seedling curtain cleans: after collecting seedling 2～3 days, the seedling curtain is scrubbed in beginning swing gently, removes mud scum and assorted algae spore; Bring into use spray gun to scrub the seedling curtain after 5～6 days, seedling curtain two ends are tightened carry out spary flushing, 10～15 days indoor culture time;
4) transportation of seedling curtain: the seedling curtain is put into incubator, and keep low temperature with the seawater ice chest, the seedling curtain needs fully moistening with seawater, seals and transports for long-distance, and is transported to northern destination and transfers marine raft formula cultivation to;
5) sea of seedling is cultivated and to be emerged: the setting of a. culture raft frame, between two floating well ropes, one of vertical steel pipe is set every 3～4 meters, and every length is 2～4 meters, tie on floating well rope with rope at the steel pipe two ends, needs 13～18 of steel pipes altogether between per 2 floating well ropes; Form 3～4 * 2～4 meters water surface area between 2 steel pipes;
B. the seedling curtain hangs: seedling curtain two ends lie on the steel pipe with lifting rope, and can put down therebetween and hang chemical fabric seedling curtain 2 * 0.3m is that 2～4 or coir rope seedling curtain 1 * 0.5m are 4～6, the stretching fully flat seawater surface that is hung on;
C. the management of seedling curtain is scrubbed: under the normal condition, the seedling curtain was scrubbed once every 1～2 day, and assurance seedling cleaning is beneficial to carries out light and effect and respiration, promotes that seedling grows fast;
D. regulate seedling curtain water layer: under the normal condition, the seedling curtain is arranged on surface layer of water, when intensity of illumination is 25000lx when above, should be with seedling curtain decline water layer to 0.3～0.5m depth of water place, and marine cultivation the through 50～70 days, seedling reaches the long upright seedling of 4cm.
The described phase of collecting seedling is that mid-May is to late May.
When spray gun was scrubbed the seedling curtain, the ascending scope of hydraulic pressure intensity was 0.3kg/cm
2～1.5kg/cm
2
When scrubbing, adopts indoor culture electrical water jetting formula flusher; The marine cultivation adopted the water jet flusher of diesel powered when scrubbing.
For solving the bottleneck problem of cultivating large seedling difficulty in the mouse tail algae seedling raising process; the south of employing ripen in advance plants vegetables and northern locality plants vegetables carries out the indoors artificial accelerating in advance; collect seedling in advance; shorten the indoor culture time; a series of standardized operation programs such as seedling takes to plunge into the commercial sea in advance, marine seedling curtain spary, assorted algae are prevented and kill off; before arriving, high temperature season forms big seed; can effectively resist the harm of marine a large amount of mix algaes and invertebrate larvas of megathermal period; improve seedling percent greatly, mouse tail algae large-scale seed breeding is achieved.
The present invention is directed to the bottleneck problem of cultivating large seedling difficulty in the above mouse tail algae artificial breeding process, the characteristics of utilizing south to plant vegetables ripe morning, and collected seedling in 50 days in advance by planting vegetables of northern artificial ripening, shorten the indoor culture time, finally can shift to an earlier date 1 wheat harvesting period and cultivate mouse tail algae large size seedling seed.Can effectively avoid the adverse effect that the marine megathermal period brings after seedling is plunged into the commercial sea, growth rate is accelerated, seedling body stalwartness, the marine cultivation through 2 months can reach the above healthy and strong mouse tail algae vertical branch seedling of 4cm, can reach the commercial seedling standard fully.
The present invention has the following advantages and good effect: broken the traditional mouse tail algae in northern sea area and collected seedling the time (late July), utilize planting vegetables of ripe morning of south, to collect seedling 50 days in advance time, shorten the indoor culture time, take early to plunge into the commercial sea, and effectively spary seedling curtain is prevented the removal of impurities algae, technology such as attachment, hang the water layer control method with suitable seedling curtain, before arriving, the marine megathermal period formed big seedling, can effectively resist the harm of adhering to of a large amount of mix algaes and animal larvas of megathermal period, guarantee nursery effect, finally can shift to an earlier date 1 wheat harvesting period and cultivate large size seedling seed, shortened the seedling culture phase, prolonged the breed phase on the mouse tail segasso sea, can effectively improve output and reduce cost.
Description of drawings
Fig. 1 is that mariculture raft frame of the present invention is provided with schematic diagram.
1. steel pipe; 2. chemical fabric seedling curtain; 3. floating well rope; 4. coir rope seedling curtain.
Embodiment
What 1) mouse tail algae planted vegetables choosing and transporting: at southern area, utilize the microscopy receptacle to determine the frond maturity, before the maturation Sheng phase arrives, to plant vegetables from the mouse tail algae of sea results and directly pack into the incubator, and keep low temperature with the seawater ice chest, suitably drench seawater, moistening to guarantee that mouse tail algae plants vegetables, then incubator is added a cover and sealed transportation and arrive northern destination;
2) preliminary treatment that plants vegetables of mouse tail algae: the mouse tail algae of transporting back from south plants vegetables, in the seawater pond, clean, choose the mouse tail algae branch that has receptacle, again sandwiching the Miao Shengzhong extension supports in top layer, pond, indoor workshop, every pond hangs supports 3 ropes, 8～15kg, hanging and supporting the female-male proportion that plants vegetables is 4～8: 1, carries out the flowing water inflation and cultivates, and water temperature is controlled at 18～22 ℃ of intensities of illumination is controlled at 3000～20000lx;
3) collection of fertilized egg: with ripe mouse tail algae plant vegetables fully clean after, evenly loose in the pond that arranges the seedling curtain in advance in the shop, it is 0.4～0.6kg that each seedling curtain plants vegetables with female mouse tail algae, male planting vegetables in 4～8: 1 ratio is arranged in pairs or groups.In hydrostatic, gather fertilized egg, in this process, constantly stir seawater or stir and plant vegetables, make fertilized egg come off fast and adhere to evenly, fertilized egg was adhered to time remaining 15～24 hours, the microscopy slide, fertilized egg is adhered to density and is reached 30～40 in every visual field (10 * 10), just can pull out planting vegetables;
4) indoor culture: the seedling curtain after fertilized egg is adhered to, the hydrostatic cultivation changes the miniflow water planting over to after 1～2 day and educates, and changes flowing water after 3～4 days over to and cultivates, water temperature is controlled at 18～22 ℃, intensity of illumination is controlled at 4000～15000lx, and at 12～14 hours sunshine time, nutrient concentration is N0 in the control seawater
3-N:3～6g/m
3, PO
4-P:0.3～0.6g/m
3, the raft frame that floats is set in the pond, make the seedling curtain place the top layer, pond all the time, seedling curtain two ends are exceptionally straight to be strained and fixed on the raft frame, so that seedling is subjected to light even;
The seedling curtain cleans: after collecting seedling 2～3 days, the seedling curtain is scrubbed in beginning swing gently, removes mud scum and assorted algae spore; Bring into use spray gun to scrub the seedling curtain after 5～6 days, seedling curtain two ends are tightened carry out the spary flushing, after 10～15 days indoor culture time, transfer marine raft formula to and cultivate;
5) sea of seedling is cultivated and emerged: a. is referring to Fig. 1, the setting of culture raft frame is provided with 1 one of vertical steel pipes every 3～4 meters between two floating well ropes 3, every length is 2～4 meters, tie on floating well rope 3 with rope at steel pipe 1 two ends, needs 13～18 of steel pipes altogether between per 2 floating well ropes 3; Form 3～4 * 2～4 meters water surface area between 2 steel pipes;
B. the seedling curtain hangs: seedling curtain two ends lie on the steel pipe 1 with lifting rope, can put down therebetween and hang chemical fabric seedling curtain 2, and the specification of chemical fabric seedling curtain 2 is 2 * 0.3m, the quantity of chemical fabric seedling curtain 2 is 2～4, coir rope seedling curtain 4 is 4～6, specification 1 * 0.5m, the stretching fully flat seawater surface that is hung on;
C. the management of seedling curtain is scrubbed: under the normal condition, the seedling curtain was scrubbed once every 1～2 day, and assurance seedling cleaning is beneficial to carries out light and effect and respiration, promotes that seedling grows fast;
D. regulate seedling curtain water layer: under the normal condition, the seedling curtain is arranged on surface layer of water, when intensity of illumination is 25000lx when above, should be with seedling curtain decline water layer to 0.3～0.5m depth of water place, and marine cultivation the through 50～70 days, seedling reaches the long upright seedling of 4cm.
Choose and delivery period that described mouse tail algae plants vegetables are that mid-May is to late May.
When spray gun was scrubbed the seedling curtain, the ascending scope of hydraulic pressure intensity was 0.3kg/cm
2～1.5kg/cm
2
When being electrical water jetting formula flusher, the marine cultivation during equipment indoor cultivation that adopts during described scrubbing with the water jet flusher of diesel powered.
The present invention is that described method is undertaken by following step in the southern area method of cultivating of collecting seedling:
1) collects seedling: at southern area,, select local sea manually to press from both sides the seedling raft and support the ripe frond of mouse tail algae, collect seedling as kind of an algae as the Dongtou County, Zhejiang;
2) collection of fertilized egg: with ripe mouse tail algae plant vegetables fully clean after, evenly loose in the pond that arranges the seedling curtain in advance in the shop, it is 0.4～0.6kg that each seedling curtain plants vegetables with female mouse tail algae, male planting vegetables: 1 ratio collocation in 4～8, in hydrostatic, gather fertilized egg, in this process, constantly stir seawater or stir and plant vegetables, make fertilized egg come off fast and adhere to evenly, fertilized egg was adhered to time remaining 15～24 hours, microscopy slide, fertilized egg adhere to density and reach 30～40 in every visual field (10 * 10), just can pull out planting vegetables;
3) indoor culture: the seedling curtain after fertilized egg is adhered to, the hydrostatic cultivation changes the miniflow water planting over to after 1～2 day and educates, and changes flowing water after 3～4 days over to and cultivates, and intensity of illumination is controlled at 4000～15000lx, and at 12～14 hours sunshine time, nutrient concentration is N0 in the control seawater
3-N:3～6g/m
3, PO
4-P:0.3～0.6g/m
3, the raft frame that floats is set in the pond, make the seedling curtain place the top layer, pond all the time, seedling curtain two ends are exceptionally straight to be strained and fixed on the raft frame, so that seedling is subjected to light even;
The seedling curtain cleans: after collecting seedling 2～3 days, the seedling curtain is scrubbed in beginning swing gently, removes mud scum and assorted algae spore; Bring into use spray gun to scrub the seedling curtain after 5～6 days, seedling curtain two ends are tightened carry out spary flushing, 10～15 days indoor culture time;
4) transportation of seedling curtain: the seedling curtain is put into incubator, and keep low temperature with the seawater ice chest, the seedling curtain needs fully moistening with seawater, seals and transports for long-distance, and is transported to northern destination and transfers marine raft formula cultivation to;
During transportation, with specification is the doubling of 2 * 0.3m chemical fabric seedling curtain, put into the foam thermal insulation case from level to level, (1 * 0.6m) approximately can put into 35 curtains to every case, and put into 4 set seawater ice chests, the middle level is put 2, top layer and is put 2, and the seedling curtain needs fully moistening with seawater, seals and transports for long-distance.Be transported to the destination through more than 20 hours, after the workshop is stablized 1～2 day, move on to marine the cultivation;
5) sea of seedling is cultivated and to be emerged: the setting of a. culture raft frame, between two floating well ropes, one of vertical steel pipe is set every 3～4 meters, and every length is 2～4 meters, tie on floating well rope with rope at the steel pipe two ends, needs 13～18 of steel pipes altogether between per 2 floating well ropes; Form 3～4 * 2～4 meters water surface area between 2 steel pipes;
B. the seedling curtain hangs: referring to Fig. 1, seedling curtain two ends lie on the steel pipe with lifting rope, and can put down therebetween and hang chemical fabric seedling curtain 2 is 2～4, and specification is 2 * 0.3m, and coir rope seedling curtain 4 is 4～6, and specification is 1 * 0.5m, the stretching fully flat seawater surface that is hung on;
C. the management of seedling curtain is scrubbed: under the normal condition, the seedling curtain was scrubbed once every 1～2 day, and assurance seedling cleaning is beneficial to carries out light and effect and respiration, promotes that seedling grows fast;
D. regulate seedling curtain water layer: under the normal condition, the seedling curtain is arranged on surface layer of water, when intensity of illumination is 25000lx when above, should be with seedling curtain decline water layer to 0.3～0.5m depth of water place, and marine cultivation the through 50～70 days, seedling reaches the long upright seedling of 4cm.
The described phase of collecting seedling is that mid-May is to late May.
When spray gun was scrubbed the seedling curtain, the ascending scope of hydraulic pressure intensity was 0.3kg/cm
2～1.5kg/cm
2
When scrubbing, adopts indoor culture electrical water jetting formula flusher; The marine cultivation adopted the water jet flusher of diesel powered when scrubbing.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (8)
1. method that northern sea area Sargassum thunbergii large size seedling seed is bred in advance is characterized in that described method undertaken by following step:
What 1) mouse tail algae planted vegetables choosing and transporting: at southern area, utilize the microscopy receptacle to determine the frond maturity, before the maturation Sheng phase arrives, to plant vegetables from the mouse tail algae of sea results and directly pack into the incubator, and keep low temperature with the seawater ice chest, suitably drench seawater, moistening to guarantee that mouse tail algae plants vegetables, then incubator is added a cover and sealed transportation and arrive northern destination;
2) preliminary treatment that plants vegetables of mouse tail algae: the mouse tail algae of transporting back from south plants vegetables, in the seawater pond, clean, choose the mouse tail algae branch that has receptacle, again sandwiching the Miao Shengzhong extension supports in top layer, pond, indoor workshop, every pond 8 * 1 * 0.8m hangs and supports 3 ropes, 8～15kg, and hanging and supporting the female-male proportion that plants vegetables is 4～8: 1, carries out the flowing water inflation and cultivates, ocean temperature is controlled at 18～22 ℃, and intensity of illumination is controlled at 3000～20000lx;
3) collection of fertilized egg: plant vegetables preliminary treatment after 1～2 day, the visible receptacle of naked eyes has ovum to hang the holder phenomenon, begin to gather fertilized egg this moment, with ripe mouse tail algae plant vegetables fully clean after, evenly loose in the pond that arranges the seedling curtain in advance in the shop, it is 0.4～0.6kg that each seedling curtain plants vegetables with female mouse tail algae, male planting vegetables is provided with according to the above ratio, gathers fertilized egg in hydrostatic, in this process, constantly stir seawater or stir and plant vegetables, make fertilized egg come off fast and adhere to evenly, fertilized egg was adhered to time remaining 15～24 hours, the microscopy slide, fertilized egg is adhered to density and is reached 30～40 in every visual field (10 * 10), just can pull out planting vegetables;
4) indoor culture: the seedling curtain after fertilized egg is adhered to, the hydrostatic cultivation changes the miniflow water planting over to after 1～2 day and educates, and changes flowing water after 3～4 days over to and cultivates, water temperature is controlled at 18～22 ℃, intensity of illumination is controlled at 4000～15000lx, and at 12～14 hours sunshine time, nutrient concentration is N0 in the control seawater
3-N:3～6g/m
3, PO
4-P:0.3～0.6g/m
3, the raft frame that floats is set in the pond, make the seedling curtain place the top layer, pond all the time, seedling curtain two ends are exceptionally straight to be strained and fixed on the raft frame, so that seedling is subjected to light even;
The seedling curtain cleans: after collecting seedling 2～3 days, the seedling curtain is scrubbed in beginning swing gently, removes mud scum and assorted algae spore; Bring into use spray gun to scrub the seedling curtain after 5～6 days, seedling curtain two ends are tightened carry out the spary flushing, after 10～15 days indoor culture time, transfer marine raft formula to and cultivate;
5) sea of seedling is cultivated and to be emerged: the setting of a. culture raft frame, between two floating well ropes, one of vertical steel pipe is set every 3～4 meters, and every length is 2～4 meters, tie on floating well rope with rope at the steel pipe two ends, needs 13～18 of steel pipes altogether between per 2 floating well ropes; Form 2～4 * 2～4 meters water surface area between 2 steel pipes;
B. the seedling curtain hangs: seedling curtain two ends lie on the steel pipe with lifting rope, and can put down therebetween and hang chemical fabric seedling curtain 2 * 0.3m is that 2～4 or coir rope seedling curtain 1 * 0.5m are 4～6, the stretching fully flat seawater surface that is hung on;
C. the management of seedling curtain is scrubbed: under the normal condition, the seedling curtain was scrubbed once every 1～2 day, and assurance seedling cleaning is beneficial to carries out light and effect and respiration, promotes that seedling grows fast;
D. regulate seedling curtain water layer: under the normal condition, the seedling curtain is arranged on surface layer of water, when intensity of illumination is 25000lx when above, should be with seedling curtain decline water layer to 0.3～0.5m depth of water place, and marine cultivation the through 50～70 days, seedling reaches the long upright seedling of 4cm.
2. method according to claim 1, choose and the delivery period that it is characterized in that described mouse tail algae plants vegetables are that mid-May is to late May.
3. method according to claim 2, when it is characterized in that spray gun is scrubbed the seedling curtain, the ascending scope of hydraulic pressure intensity is 0.3kg/cm
2～1.5kg/cm
2
4. method according to claim 3 is characterized in that adopting when indoor culture is scrubbed electrical water jetting formula flusher; The marine cultivation adopted the water jet flusher of diesel powered when scrubbing.
5. method that northern sea area Sargassum thunbergii large size seedling seed is bred in advance is characterized in that described method undertaken by following step:
1) collects seedling: at southern area, select local sea manually to press from both sides the seedling raft and support the ripe frond of mouse tail algae, collect seedling as kind of an algae;
2) collection of fertilized egg: with ripe mouse tail algae plant vegetables fully clean after, evenly loose in the pond that arranges the seedling curtain in advance in the shop, it is 0.4～0.6kg that each seedling curtain plants vegetables with female mouse tail algae, male planting vegetables: 1 ratio collocation in 4～8, in hydrostatic, gather fertilized egg, in this process, constantly stir seawater or stir and plant vegetables, make fertilized egg come off fast and adhere to evenly, fertilized egg was adhered to time remaining 15～24 hours, microscopy slide, fertilized egg adhere to density and reach 30～40 in every visual field (10 * 10), just can pull out planting vegetables;
3) indoor culture; Seedling curtain after fertilized egg is adhered to, the hydrostatic cultivation changes the miniflow water planting over to after 1～2 day and educates, and changes flowing water after 3～4 days over to and cultivates, and intensity of illumination is controlled at 4000～15000lx, and at 12～14 hours sunshine time, nutrient concentration is N0 in the control seawater
3-N:3～6g/m
3, PO
4-P:0.3～0.6g/m
3, the raft frame that floats is set in the pond, make the seedling curtain place the top layer, pond all the time, seedling curtain two ends are exceptionally straight to be strained and fixed on the raft frame, so that seedling is subjected to light even;
The seedling curtain cleans: after collecting seedling 2～3 days, the seedling curtain is scrubbed in beginning swing gently, removes mud scum and assorted algae spore; Bring into use spray gun to scrub the seedling curtain after 5～6 days, seedling curtain two ends are tightened carry out spary flushing, 10～15 days indoor culture time;
4) transportation of seedling curtain: the seedling curtain is put into incubator, and keep low temperature with the seawater ice chest, the seedling curtain needs fully moistening with seawater, seals and transports for long-distance, and is transported to northern destination and transfers marine raft formula cultivation to;
5) sea of seedling is cultivated and to be emerged: the setting of a. culture raft frame, between two floating well ropes, one of vertical steel pipe is set every 3～4 meters, and every length is 2～4 meters, tie on floating well rope with rope at the steel pipe two ends, needs 13～18 of steel pipes altogether between per 2 floating well ropes; Form 3～4 * 2～4 meters water surface area between 2 steel pipes;
B. the seedling curtain hangs: seedling curtain two ends lie on the steel pipe with lifting rope, and can put down therebetween and hang chemical fabric seedling curtain 2 * 0.3m is that 2～4 or coir rope seedling curtain 1 * 0.5m are 4～6, the stretching fully flat seawater surface that is hung on;
C. the management of seedling curtain is scrubbed: under the normal condition, the seedling curtain was scrubbed once every 1～2 day, and assurance seedling cleaning is beneficial to carries out light and effect and respiration, promotes that seedling grows fast;
D. regulate seedling curtain water layer: under the normal condition, the seedling curtain is arranged on surface layer of water, when intensity of illumination is 25000lx when above, should be with seedling curtain decline water layer to 0.3～0.5m depth of water place, and marine cultivation the through 50～70 days, seedling reaches the long upright seedling of 4cm.
6. method according to claim 5 is characterized in that the described phase of collecting seedling is that mid-May is to late May.
7. method according to claim 6, when it is characterized in that spray gun is scrubbed the seedling curtain, the ascending scope of hydraulic pressure intensity is 0.3kg/cm
2～1.5kg/cm
2
8. method according to claim 7 is characterized in that adopting when indoor culture is scrubbed electrical water jetting formula flusher; The marine cultivation adopted the water jet flusher of diesel powered when scrubbing.
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