CN1973613A - Temperature controlling low-salinity sargassum thunbergii seedling growing process - Google Patents
Temperature controlling low-salinity sargassum thunbergii seedling growing process Download PDFInfo
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- CN1973613A CN1973613A CNA2006100707658A CN200610070765A CN1973613A CN 1973613 A CN1973613 A CN 1973613A CN A2006100707658 A CNA2006100707658 A CN A2006100707658A CN 200610070765 A CN200610070765 A CN 200610070765A CN 1973613 A CN1973613 A CN 1973613A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The temperature controlling low-salinity Sargassum thunbergii seedling growing process includes regulating and controlling water temperature in 17-23 deg.c by means of forced air cooling and water cooling; compounding optimized-salinity nutritious slat solution with salinity of 2.3-2.7 wt%, NaNO3 concentration of 4 mg/l and KH2PO4 concentration of 0.4 mg/l through adding fresh water and measuring specific weight with a gravimeter; and collecting seedling and seedling culturing through selecting and treating seed Sargassum thunbergii, controlling ratio between female and male Sargassum thunbergii, collecting, attaching with medium, controlling salinity and temperature, controlling illumination and nutritious salts and refreshing sea water. The present invention can culture Sargassum thunbergii seedlings in high speed.
Description
Technical field:
The invention belongs to Sargassum thunbergii offspring cultivation technology, is the seedling-cultivating method of a kind of mouse tail algae under temperature control, low salinity.
Background technology:
Mouse tail algae is a kind of emerging economical alga, can be used as the best bait of sea cucumber.Owing to be subjected to the fast-developing influence of holothruian cultures industry, artificial a large amount of collections make this algae natural resources tend to exhausted.
In recent years, people gathered wild mouse tail algae and directly carry out raft formula examination and support, and proved that mouse tail algae can propagate artificially.
Nearly 1-2 has the people to carry out the seed rearing test at normal temperatures, and evidence can obtain the seedling of 1-2 millimeter.But the seedling size is very irregular, and emergence rate accounts for about 1/3 of whole seedling curtain; Especially in summer high temperature season, mashed seedling, seedling, the seedling situation that stops growing of taking off are very outstanding.
Summary of the invention:
The purpose of this method is exactly to create the stable temperature controlling low-salinity sargassum thunbergii seedling growing process of a cover, solve seed produce unstable, seedling is childlike, especially in mashed seedling of summer high temperature seedling in season and dormant problem.
This method takes following technical scheme to realize that the whole technique step mainly comprises: the allotment of the adjusting of water temperature and control, optimal salinity and nutritive salt, collect seedling, seed rearing:
1, the adjusting of water temperature and control: when between growing seedlings, growing seedlings, adopt air-cooled, water-cooling pattern, will cultivate water temperature and be controlled at 17-23 ℃.
2, the allotment of optimal salinity and nutritive salt: to add the fresh water method, to modulate by densimeter, salinity is controlled at 23-27 ‰; Inwardly add N, P nutritive salt (NaNO by calculating water body
3, KH
2PO
4), make culture fluid N, P concentration be controlled at 4mg/L, 0.4mg/L respectively.
3, collect seedling: the preparation and the utilization of 1) planting algae: the ovum phenomenon occurs hanging as the fully ripe sign of mouse tail algae with female receptacle, the male and female frond that collection is fully ripe also cleans, with frond dry in the shade stimulation 20-60 minute, female, male frond proportion control was about 1: 8 before collecting seedling; 2) water temperature of collecting seedling is controlled at 17-21 ℃; 3) salinity water of collecting seedling is controlled at 25-27 ‰; 4) adopt brown curtain, vinylon curtain or polyethylene rope as growing seedlings adherance; 5) seedling is gathered: adherance is tiled in the pond of collecting seedling, and collection fertilized egg is watered equably and is spread across in the pond of collecting seedling, and falls on the adherance; Also the kind algae behind the dry in the shade stimulation evenly can be laid on the adherance, swing and move kind of an algae after 24 hours gently, make fertilized egg break away from receptacle, to plant algae then takes out, microscopy fertilized egg adhere to density, as the density deficiency, can replenish fertilized egg until reaching requirement.
4, seed rearing: 1) cultivate water temperature and be controlled at 17-23 ℃; 2) seawater salinity is adjusted to 23-27 ‰; 3) add N, P nutritive salt: N concentration is controlled at 3-5mg/L, and P concentration is controlled at 2-4mg/L; 4) illumination: adopt natural daylight to grow seedlings, light intensity is controlled at 4000-100001x; Low hot house adopts fluorescent light source, and light intensity is controlled at 3000-50001x; 5) scrubbed one time the seedling curtain every 2-4 days, change once new water, or flowing water is cultivated; When 6) seed is cultured to 5mm, plunges into the commercial sea and support temporarily.
Present technique and prior art contrast are characterized in:
1, adopts temperature control technique to carry out the seed rearing of mouse tail algae first, make mouse tail algae seedling be in optimum growth temp all the time during the broiling summer at 17-23 ℃.
2, adopt low salinity water (23-26 ‰) to cultivate mouse tail algae seedling first, solve the seed poor growth, solve the 1-2 millimeter seedling situation that stops growing especially.Experimental results show that mouse tail algae has extremely strong anti-low salinity ability, under 20-25 ‰ salinity, the growth of mouse tail algae is splendid, and disease-resistant mashed ability is also extremely strong.
3, adopting temperature control and low salinity to carry out mouse tail algae grows seedlings, can in 40-50 days time, cultivate size evenly, the big specification Sargassum thunbergii offspring of long 5 millimeters of body, density suitable (15-25 strain/centimetre), the low salinity seedling-cultivating method of temperature control can make that growth of seedling is fast, healthy, emergence rate is high.
Embodiment:
Be described in detail the technology contents of this method below by embodiment:
The whole technique step mainly comprises: the allotment of the adjusting of water temperature and control, optimal salinity and nutritive salt, collect seedling and seed rearing:
1, the adjusting of water temperature and control: when between small-sized growing seedlings, growing seedlings, adopt air cooling way will cultivate water temperature and be controlled at 19 ± 1 ℃.When grow seedlings in the large-scale production workshop, can adopt water-cooling pattern to make water at low temperature, by setting up a distribution reservoir, it is standby that water temperature is adjusted to 19 ± 1 ℃ of the optimum temperatures of growing seedlings, and by pipeline or water pump moisture is fitted on the cultivation pond when needing.
2, the allotment of optimal salinity and nutritive salt: when between small-sized growing seedlings, growing seedlings, adopt bucket or tank to contain seawater and also inwardly add fresh water, modulate best salinity 25 ± 1 ‰ by densimeter.When grow seedlings in the large-scale production workshop, in distribution reservoir, add fresh water, salinity is modulated to optimum range 25 ± 1 ‰.Inwardly add N, P nutritive salt (NaNO by calculating water body
3, KH
2PO
4), make culture fluid N, P concentration be controlled at 4mg/L, 0.4mg/L respectively.
3, collect seedling: plant 1) that algae is prepared and utilization: with female receptacle the ovum phenomenon appears hanging as the fully ripe sign of mouse tail algae, the male and female frond that collection is fully ripe also cleans, select the female frond of rinsing well of hanging ovum, cut the vegetative shoot part of frond base portion, only leave strip receptacle part.Select ripe male frond, remove base portion vegetative shoot part, with male and female frond dry in the shade stimulation 30 minutes, female, male frond proportion control was about 1: 8; 2) control of water temperature and salinity: at distribution reservoir water temperature and salinity are modulated to 19 ± 1 ℃ and 25 ± 1 ‰ respectively, are injected into the pond of collecting seedling and collect seedling; 3) use of adherance: adopt brown curtain, vinylon curtain material as growing seedlings adherance; 4) seedling is gathered: will collect seedling curtain or seedl-picking rope lie in the pond of collecting seedling, and are spread across in the water of tiling seedling curtain or rope concentrating the fertilized egg of gathering to water equably, and it is fallen on collect seedling curtain or the rope; Also the kind algae behind the dry in the shade stimulation evenly can be laid on seedling curtain or the rope, swing and move kind of an algae after 24 hours gently, make fertilized egg fully break away from receptacle, adhere on the adherance, to plant algae then and take out, microscopy fertilized egg adhere to density, as the density deficiency, can adopt and concentrate the fertilized egg liquid of gathering to replenish, in reaching 100 times of visuals field of microscope, on average contain till 8-20 the healthy fertilized egg requirement.
4, seed rearing: 1) temperature control: the seed rearing water temperature is controlled at 19 ± 1 ℃; 2) salinity control: seawater salinity is adjusted to 25 ± 1 ‰; 3) add N, P nutritive salt, N concentration is controlled at 3-5mg/L, and P concentration is controlled at 2-4mg/L; 4) illumination: adopt natural daylight to grow seedlings, light intensity is controlled at 6000-200001x; Low hot house adopts fluorescent light source, and light intensity is controlled at 3000-70001x; 5) scrub a seedling curtain (rope) every 2-4 days, change once new water.Later stage of growing seedlings also can be adopted the flowing water training method; When 6) seed is cultured to 5mm, plunges into the commercial sea and support temporarily.
Claims (1)
1, a kind of temperature controlling low-salinity sargassum thunbergii seedling growing process, comprise water temperature adjusting and control, optimal salinity and nutritive salt allotment, collect seedling and seed rearing: it is characterized in that described water temperature is regulated and control; Its optimum water temperature is controlled at 17-23 ℃; Described optimal salinity: salinity is controlled at 23-27 ‰.
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CNA2006100707658A CN1973613A (en) | 2006-12-18 | 2006-12-18 | Temperature controlling low-salinity sargassum thunbergii seedling growing process |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101263780B (en) * | 2008-04-24 | 2010-06-09 | 山东省海水养殖研究所 | Method for breeding Sargassum thunbergii large size seedling in northern sea area ahead of time |
CN105532421A (en) * | 2015-12-16 | 2016-05-04 | 胡彩军 | Method for artificially producing sargassum fusiforme seedlings in freshwater |
CN105993907A (en) * | 2016-06-21 | 2016-10-12 | 山东省海洋生物研究院 | Seedling collecting method and early breeding method of sargassum thunbergii |
CN109729968A (en) * | 2019-03-19 | 2019-05-10 | 山东省海洋生物研究院 | The proliferation big specification breeding method of sargassum thunbergii seedling in a kind of sea jungle nursery |
CN111492966A (en) * | 2020-05-07 | 2020-08-07 | 海南热带海洋学院 | Seedling growing method for tylophora |
CN116267578A (en) * | 2021-09-24 | 2023-06-23 | 福建省水产研究所(福建水产病害防治中心) | Rapid sprouting method for asparagus seedling cut segments and application of low-salt culture solution |
-
2006
- 2006-12-18 CN CNA2006100707658A patent/CN1973613A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101263780B (en) * | 2008-04-24 | 2010-06-09 | 山东省海水养殖研究所 | Method for breeding Sargassum thunbergii large size seedling in northern sea area ahead of time |
CN105532421A (en) * | 2015-12-16 | 2016-05-04 | 胡彩军 | Method for artificially producing sargassum fusiforme seedlings in freshwater |
CN105993907A (en) * | 2016-06-21 | 2016-10-12 | 山东省海洋生物研究院 | Seedling collecting method and early breeding method of sargassum thunbergii |
CN109729968A (en) * | 2019-03-19 | 2019-05-10 | 山东省海洋生物研究院 | The proliferation big specification breeding method of sargassum thunbergii seedling in a kind of sea jungle nursery |
CN111492966A (en) * | 2020-05-07 | 2020-08-07 | 海南热带海洋学院 | Seedling growing method for tylophora |
CN116267578A (en) * | 2021-09-24 | 2023-06-23 | 福建省水产研究所(福建水产病害防治中心) | Rapid sprouting method for asparagus seedling cut segments and application of low-salt culture solution |
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