CN101606499A - A kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding - Google Patents

A kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding Download PDF

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Publication number
CN101606499A
CN101606499A CNA2009100171201A CN200910017120A CN101606499A CN 101606499 A CN101606499 A CN 101606499A CN A2009100171201 A CNA2009100171201 A CN A2009100171201A CN 200910017120 A CN200910017120 A CN 200910017120A CN 101606499 A CN101606499 A CN 101606499A
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jellyfish
hydrula
water
feeding
parent
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谭福祎
唐聚德
张术森
孙永军
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Shandong Homey Aquatic Development Co Ltd
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Shandong Homey Aquatic Development Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The present invention relates to the method that a kind of jellyfish hybrid breeding is survived the winter and the centre is bred.It is gathered the wild jellyfish of Yancheng, Jiangsu Haizhou Wan and does that the male parent parent stings, the maternal parent of the wild work in quiet bay, Shandong stings, through store up, the jellyfish kind stings to lay eggs and carries out low temperature preservation and overwintering culture, the short-term warming of hydrula with 3-10 ℃ of collection, the cultivation of hydrula, the control water temperature of hatching, floating wave larva and keep final water temperature and carry out hydrula strobilation, temperature control for 18 ℃, throw something and feed, change water, carry out ephyra and cultivate to the commercial seedling specification.The present invention makes jellyfish hybrid breeding technology obtain breaking through, solved the technical problem that the jellyfish germplasm is degenerated, it is strong, high temperature resistant that the jellyfish kind of cultivating with this method has adaptability, and resistance against diseases is strong, fast growth, through growth test, after this kind jellyfish grows up to, the body colour redness, physiological datas such as the umbrella footpath of jellyfish, body weight, gelatinous layer, oral arm have obvious raising, on average increase production more than 20%.

Description

A kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding
Technical field
The present invention relates to the breed of a kind of jellyfish, especially a kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding.
Background technology
Jellyfish (Rhopilema esculetum Kishinouye) is the large-scale water warm jellyfish class that is grown in the life of swimming in the ocean, be diploblastica, be under the jurisdiction of Coelenterata (Coelenterate), Scyphozoa (Scyphomedusae), the female order (Rhizostomeae) of root saliva, the female section (Rhizostomadae) of root saliva, jellyfish belongs to (Rhopilema).The young of jellyfish inhabits the sea area of river mouth degree of saltiness water intersection.Than the place, river mouth of big rivers, be the place of young breeding of stinging and growth in each sea area.Each breeds the place, and because of the geographical position difference, time and velocity contrast that spring, the seawater water temperature was gone up are bigger, therefore, each sea area jellyfish concentrate breeding, vegetative period and the migration route that swims also inequality.Coastal from south to north in China, formed multiple endemic population, as colonies such as East Guangdong, the south of Fujian Province, Min Dong, Zhe Nan, Hangzhou Wan, Haizhou Wan, Laizhou Wan, Bohai Sea Gulf and Liaodong Wans.
In order to satisfy the active demand in market, jellyfish is cultured and has formed upsurge in the whole nation at present, becomes a new industry in China's mariculture.But continuous development along with the jellyfish aquaculture, the problem that faces is outstanding day by day, survival rate and growth rate as jellyfish are descending year by year, unfolding before degree also is not so good as of jellyfish, the jellyfish of results goes out degradation under the skin rate, the appearance of these problems and the germplasm of jellyfish are degenerated confidential relation, and cultivating new kind by jellyfish hybridization is to solve the effective way that the jellyfish germplasm is degenerated, but survives the winter about the jellyfish hybrid breeding at present and the middle technology of breeding is not appeared in the newspapers as yet.
Summary of the invention
In order to solve the technical problem that existing jellyfish germplasm is degenerated, the invention provides the method that a kind of jellyfish hybrid breeding is survived the winter and the centre is bred.
The technical solution adopted for the present invention to solve the technical problems is: a kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding, it is characterized in that: this method is stung and is laid eggs and hatching, the collection of floating wave larva, the cultivation of hydrula and five stages of cultivation of low temperature preservation and overwintering culture, hydrula strobilation and ephyra by its step storing up of being divided into that the jellyfish parent stings, jellyfish kind, and it is as follows to show division:
What 1, the jellyfish parent stung stores up
Jellyfish first sexually matured reach 9 months by the end of August at the beginning of, the jellyfish of gathering the different sea areas of individual weight more than 5kg stings as male parent, maternal parent, by female-male proportion 2-3: 1 goes into the pond, the control water temperature is 20-26 ℃ stores up, and stores up in the phase, changes water every day 2 times, morning 100%, the dusk 50%; Respectively throw something and feed in every morning, afternoon artemia nauplii 1 time, daily ration, feeding quantity with the 1.5-2h that throws something and feeds after in the water body no obvious artemia nauplii distribute and be as the criterion;
2, the jellyfish kind is stung and is laid eggs and hatching
2.1 retaining and water treatment
To obtain ovum pond outwash before growing seedlings, inject fresh filtering sea, 1 meter of the depth of water, every cube of water body adds 4-6g/m 3EDTA, and inflate stand-by;
2.2 lay eggs
Select time is 2:00-3:00 in morning, stops inflation, the parent who cultivates is bitten by male and female obtained in the ovum pond than immigration in 3: 1, and density is controlled at 1-2/m 3Smart back 3h is bitten and lay eggs, arranged to the parent, and it is shifted out from obtain the ovum pond; Treat that Chi Shui leaves standstill 0.5h, upper strata 65cm water siphon is discharged, do not stir Chi Shui during draining, in order to avoid fertilized egg runs off in a large number, and draining will be finished in 1h; Afterwards, add the isothermal filtering sea again to original position, and additional EDTA, little inflation hatching;
2.3 hatching
Under water temperature 20-24 ℃ condition, fertilized egg begins the spilting of an egg through 0.5h, when growing to blastocoele, just floats gradually at the bottom of the pond, and fertilized egg is through 6-8h bud into floating wave larva;
3, the collection of floating wave larva
With floating wave larvae cultivation 20-24h, throw in adherance, this adherance is suspended in the pond, the 30cm at the bottom of the pond of its lower floor, be advisable just to be submerged in the underwater in the upper strata, and injected volume is 3 groups/m 2, stop inflation between the floating wave larva setting stage, add seawater to the water surface every 2-3 days and exceed adherance 25cm; Reach 1-1.5/cm in adherance spatfall density 2The time, begin to change water and trace inflation;
4, the cultivation of hydrula and low temperature are preserved and overwintering culture
4.1 the cultivation of hydrula
After becoming 4 tentacle hydrulas attached to the floating wave larvae development on the adherance, throw something and feed this kind jellyfish floating wave larva or shellfish trochophore, the time of throwing something and feeding begins after 3-4 days for throwing in adherance, throw something and feed every day 1 time, daily ration, feeding quantity be the spatfall amount 5-6 doubly; Grow after 5-6 days to tool 8 tentacle hydrulas in mid-term, the artemia nauplii of throwing something and feeding, bait throwing in per 2 days once, daily ration, feeding quantity with throw something and feed the back 1-2h in no residual bait be as the criterion, and throw something and feed and inflate 2h after change water, quantity of exchanged water is the 1/3-1/2 in full pond; The later stage hydrula for tool 16 tentacles is grown in cultivation through 8-10 days;
4.2 the overwintering culture of hydrula and low temperature are preserved
After hydrula developed into 16 tentacles, the trend of progressively lowering the temperature along with outer seawater was controlled at 3-10 ℃ with the cultivation water temperature of hydrula, and hydrula is carried out overwintering culture and low temperature is preserved; During this period, shut out the light with black plastic film, the control intensity of illumination is no more than 500lux; With the artemia nauplii is that bait is thrown something and fed, and controls daily ration, feeding quantity and the number of times of throwing something and feeding, and throws something and feeds in 1 week 1 time in the time of 3-5 ℃, throws something and feeds weekly in the time of 6-10 ℃ 2 times, and feeding volume is 8-10 a times of hydrula quantity; Changed water once in 5-7 days, quantity of exchanged water is 1/3-1/2, changes before the water dirt at the bottom of the first sucking-off pond, adds the equivalent fresh seawater again;
5, the cultivation of the strobilation of hydrula and ephyra
5.1 the strobilation of hydrula
Next year, the mid or late March water temperature was gone up to more than 10 ℃ the time, remove black plastic film, improve indoor intensity of illumination, the reproduction function of hydrula is recovered gradually, and strengthen daily ration, feeding quantity and the number of times of throwing something and feeding, throw something and feed every day 4 times, no obvious artemia nauplii distributes daily ration, feeding quantity in the water body, hydrula is crocus and is as the criterion after with the 1h that throws something and feeds; Treat that water temperature is raised to about 13 ℃, fall the pond when hydrula transverse fissure begins, and falling behind the pond to heat up 2 ℃ every day, final water temperature maintains 18 ℃, same hydrula repeatedly carries out strobilation during the strobilation, breaks away from parent in succession, produces the ephyra of free swimming;
5.2 the cultivation of ephyra
Under 18-22 ℃ of condition, carry out the cultivation of ephyra, density is cultivated in control, the trace inflation, cultivating through 7-10d becomes umbrella and reaches directly that the 10mm left and right sides is young bites, in the nurturing period, along with the growth of ephyra strengthens the number of times of throwing something and feeding, daily ration, feeding quantity and quantity of exchanged water gradually, artemia nauplii 2-3 time of throwing something and feeding every day, each individual daily ration of feeding 20-300 artemia nauplii; Change water 2-3 every day, and quantity of exchanged water is the 1/2-3/4 in full pond; Cultivating through 7-10d becomes the children that umbrella directly reaches about 20mm and bites again, and reaches the commercial seedling specification, and young biting cultivated the children and bitten, and its food ration heightens, and oxygen consumption and metabolite increase gradually, change water 3-4 time in this every day nurturing period, and quantity of exchanged water is 3/4 of full pond; Throw something and feed every day artemia nauplii 3-4 time, each individual daily ration of feeding 1000-1500 artemia nauplii; And keep being limited to 4mg/L under the water body dissolved oxygen, being limited to 0.15mg/L on the ammoniacal nitrogen, the pH scope is 7.5-8.5.
The jellyfish in the different sea areas of described collection stings as male parent, maternal parent, and its male parent parent stings and is that it is that the wild jellyfish in bay is made tranquil in Shandong that the wild jellyfish of Yancheng, Jiangsu Haizhou Wan, maternal parent sting.
Jellyfish hybrid breeding of the present invention is survived the winter and method is bred in the centre, passed through the cultivation that the jellyfish parent stings, the jellyfish kind is stung and is laid eggs and hatching, the collection of floating wave larva, the cultivation of hydrula and low temperature are preserved and are survived the winter, five stage steps of the cultivation of hydrula strobilation and ephyra, jellyfish by gathering different sea areas is as male parent, maternal parent stings, make jellyfish hybrid breeding technology obtain breaking through, especially solved the technical problem that the jellyfish germplasm is degenerated, it is strong that the jellyfish kind of using this method to cultivate has adaptability, high temperature resistant, resistance against diseases is strong, fast growth.Through contrast, after the jellyfish seedling of cultivation grows up to, the body colour redness, physiological datas such as the umbrella footpath of jellyfish, body weight, gelatinous layer, oral arm have obvious raising, on average increase production more than 20%.
Embodiment
Below in conjunction with embodiment the present invention is described further.
A kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding, and stings and lays eggs and hatching, the collection of floating wave larva, the cultivation of hydrula and five stages of cultivation of low temperature preservation and overwintering culture, hydrula strobilation and ephyra by its step storing up of being divided into that the jellyfish parent stings, jellyfish kind:
1, the jellyfish parent cultivation of stinging
The first sexual maturity time of jellyfish is at the beginning of reaching 9 months by the end of August, gather the wild jellyfish of Yancheng, Jiangsu Haizhou Wan as male parent, the wild jellyfish in quiet bay, Shandong is as female parent, be elected to be body weight that the parent stings more than 5kg, big to guarantee that the parent is bitten fish brood amount, the reproduction rate height is laid eggs early, and fertilization rate, incubation rate, survival rate are high.Go into the pond at 3: 1 by female-male proportion, the control water temperature is stored up for 20-26 ℃, and during storing up, change water 2 general every day, morning 100%, dusk 50%.Every morning, 1 artemia nauplii of each bait throwing in afternoon, daily ration, feeding quantity with the 1.5-2h that throws something and feeds after in the water body no obvious artemia nauplii distribute and be as the criterion.
2, the jellyfish kind is stung and is laid eggs and hatching
2.1 retaining and water treatment
Grow seedlings and to obtain ovum pond outwash noon before that day, inject 1 meter of fresh filtering sea.Every cube of water body adds 5g/m 3EDTA, and inflate stand-by;
2.2 lay eggs
Select time for morning 2:00-3:00 stop inflation, the parent is bitten to move into than 3: 1 by male and female obtains in the ovum pond, the parent of laying eggs bite density be controlled at 1-2 individual/m 3, the parent is bitten to move into to obtain and is laid eggs, arrange behind the about 1h in ovum pond smart, and the 3h afterwards of laying eggs is bitten the parent from spawning pond and to be shifted out, and treats that Chi Shui leaves standstill 0.5h, with the discharge of upper strata 65cm water, does not as far as possible stir Chi Shui during draining with siphonage, in order to avoid fertilized egg runs off in a large number, and finishes in 1h.Afterwards, add the isothermal filtering sea again to original position, and additional EDTA, little inflation hatching,
2.3 hatching
Under 20~24 ℃ of water temperatures, fertilized egg begins the spilting of an egg through 0.5h, when growing to blastocoele, just at the bottom of the pond, float gradually, fertilized egg is grown through 6~8h and is the floating wave larva, and use volumetric method to carry out quantitatively this moment, and hatching process is taken a sample at the bottom of the pond every 30min and examined under a microscope the embryonic development situation.
3, the collection of floating wave larva
The floating wave larva is cultivated under natural temperature, swimming ability, left-handed rotation, how activity at the bottom of the pond, the swimming ability strengthens after several hours, distributes for helping larva, can the trace inflation cultivate, when floating wave larvae cultivation during to 20-24h, should in time throw in adherance, this adherance is suspended in the pond, the 30cm at the bottom of the pond of its lower floor, be advisable just to be submerged in the underwater in the upper strata, injected volume is 3 groups/m 2, stop inflation this moment, added an amount of seawater every 2 days later on, adds to the water surface and exceed adherance 25cm.During settlement and metamorphosis, floating wave larva front end adheres to formation pedal disk and shank, and the rear end forms mouth and tentacle, does not run into adherance during as metamorphosis, and then abnormal under floating state, shank is upwards hung by the feet and bubbled through the water column.If the floating wave larva lazy weight that obtains for the first time, the floating wave larva that can will newly hatch for several times continuously goes in the pond with 400 purpose net cage collection, has so both increased spatfall quantity, can be used as the bait that has adhered to 4 tentacle hydrulas again.When average spatfall density reaches 1~1.5/cm 2The time, no longer collection is gone into the floating wave larva, can begin to change water and trace inflation.
4, the cultivation of hydrula and low temperature are preserved and overwintering culture
4.1 the cultivation of hydrula
The floating wave larva adheres to after 1 day that to grow be 4 tentacle hydrulas, begins to ingest.This moment, individuality was very little, all can cause mortality if bait throwing in is untimely or open-mouthed bait is uncomfortable.Present embodiment is mainly thrown in this kind jellyfish floating wave larva or shellfish trochophore as open-mouthed bait.The time of throwing something and feeding should begin after 3 days at the input adherance, throws something and feeds every day 1 time, and daily ration, feeding quantity is 5-6 a times of spatfall amount.Can grow after 5-6 days to tool 8 tentacle hydrulas in mid-term, the long 0.5-0.8mm of body begins the artemia nauplii of throwing something and feeding this moment, bait throwing in per 2 days once, daily ration, feeding quantity is as the criterion with no residual bait in the 1-2h that throws something and feeds, and throw something and feed and inflate 2 hours after change water, quantity of exchanged water is the 1/3-1/2 in full pond; Pass through 8-10 days cultivation again, grow later stage hydrula for tool 16 tentacles, the long 1-3mm of body, this moment, hydrula was grown normal.
4.2 the low temperature of hydrula is preserved and overwintering culture
Because time of artificial culture hydrula is the 9-10 month, the time of cultivating the young next year is the 3-6 month, during about 6 months at interval, and have one to survive the winter the stage.During this period, carry out low temperature to hydrula preserves and overwintering culture.Specific practice is in the present embodiment: after hydrula develops into 16 tentacles, the trend of progressively lowering the temperature along with outer seawater adopts the progressively method of cooling, make it adapt to low temperature, and water temperature is controlled at 3-10 ℃, reduce its physiological metabolism level, enter the diapause state, hydrula is carried out low temperature preserve and overwintering culture.During low temperature preservation and overwintering culture, adopt black plastic film to block the workshop door and window, make the workshop intensity of illumination be no more than 500lux, the algae that prevents to mix etc. multiplies in a large number at Seedling culturing device, improves the hydrula survival rate, also helps the podocyst reproduction.In the winter time, the indoor heating installation lagging facility that is provided with does not freeze to keep the pond; When spring, indoor water temperature reached more than 10 ℃, adopt to increase and change the waterside number, make indoor water temperature reach the natural waters water temperature gradually; Summer hydrula keep a full stand of seedings, indoor employing is placed ice cube and is lowered the temperature, and makes in hydrula keeps 10 ℃, bites seedling production demand can guarantee 7-8 month children.
Low temperature preserve and overwintering culture during be bait with the artemia nauplii, daily ration, feeding quantity and number of times are controlled in good time, generally throw something and feed in 1 week 1 time in the time of 3-5 ℃, throw something and feed weekly in the time of 6-10 ℃ 2 times, feeding volume be hydrula quantity 8-10 doubly.In preservation of hydrula low temperature and overwintering culture process, be also noted that what and cycle of quantity of exchanged water, take in the present embodiment to change water once in 5-7 days, change water 1/3-1/2 after general each the throwing something and feeding.Change before the water dirt at the bottom of the first sucking-off pond, add the equivalent fresh seawater again.
5, the cultivation of the strobilation of hydrula and ephyra
5.1 the strobilation of hydrula
Next year, the mid or late March water temperature was gone up to more than 10 ℃ the time, and the reproduction function of hydrula is recovered gradually, enters the new developmental stage.Remove black plastic film on the window this moment, improves indoor intensity of illumination, to improve the strobilation incidence.
Water temperature is raised to 13 ℃ of left and right sides hydrulas and begins transverse fissure, horizontal hanging promptly occur from top to bottom and form strobila in holder below the hydrula tentacle.Split joint when dark, its shape is like a folded plate, will fall the pond this moment.For in the short time (about general two weeks), turning out young jellyfish, can be in falling behind the pond to heat up about 2 ℃ every day, final water temperature maintains 18 ℃, and hydrula breaks away from parent from top to bottom in succession during the strobilation, produces the ephyra of free swimming.The quantity that discharges ephyra is directly proportional with the size of hydrula, and nutrition is the principal element that influences the hydrula growth, after so hydrula is survived the winter, should pay attention to the abundant supply of bait especially, strengthen its daily ration, feeding quantity and the number of times of throwing something and feeding, throw something and feed every day 4 times, because of hydrula is crocus on a full stomach, be pale asphyxia when hungry, thus daily ration, feeding quantity with the 1h that throws something and feeds after no obvious artemia nauplii distribution in the water body, hydrula be crocus and be as the criterion.The ephyra that the strobilation first of each hydrula produces, can reach 20 at most surplus, minimum 4, general 5-10.Last ephyra reverts to the hydrula state again, through growing of a period of time after breaking away from, strobilation once more, and same hydrula can repeatedly carry out strobilation, but the quantity that forms ephyra reduces gradually, finally once only discharges 1-2.
5.2 the cultivation of ephyra
Nascent ephyra water white transparency, diameter 2-4mm.The cultivation of ephyra will be carried out under 18-22 ℃ condition, controls it and cultivates density with 10000/m 3Be advisable, and the trace inflation; Can be grown to umbrella through 7-10d and reach directly that the 10mm left and right sides is young bites; Be grown to the children that umbrella directly reaches about 20mm through 7-10d again and bite, reach the commercial seedling specification.Along with growing that the young children of biting is bitten, its oxygen demand and ammonia excretion amount sharply rise.Therefore, in cultivating process, keep water quality fresh most important, be limited to 4mg/L under the water body dissolved oxygen, be limited to 0.15mg/L on the ammoniacal nitrogen, the pH scope is 7.5~8.5.
The stage cultivating and managing that ephyra bites to the children sees the following form.
Stage Inferior/d Food species Individual day feeding volume Change the waterside number Quantity of exchanged water
Ephyra is to young biting the initial stage ??2 Artemia nauplii ??20-40 ??2 ??1/2V
Bite to 10mm is young ??2-3 Artemia nauplii ??200-300 ??2-3 ??3/4V
Bite to 20mm children ??3-4 Artemia nauplii ??1000-1500 ??3-4 ??3/4V
Poly-by the above-mentioned step, ephyra generally can reach more than 80% to young survival rate of biting the stage, and biting the survival rate in stage to the children can reach more than 60%.
Jellyfish hybrid breeding of the present invention is survived the winter and method is bred in the centre, sting as male parent, maternal parent by the jellyfish of gathering different sea areas, make jellyfish hybrid breeding technology obtain breaking through, solved the technical problem that the jellyfish germplasm is degenerated, it is strong that the jellyfish seed of cultivating has adaptability, high temperature resistant, resistance against diseases is strong, fast growth.Through growth test, after this kind jellyfish grows up to, the body colour redness, physiological datas such as the umbrella footpath of jellyfish, body weight, gelatinous layer, oral arm have obvious raising, on average increase production more than 20%.

Claims (2)

1, a kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding, it is characterized in that: this method is stung and is laid eggs and hatching, the collection of floating wave larva, the cultivation of hydrula and five stages of cultivation of low temperature preservation and overwintering culture, hydrula strobilation and ephyra by its step storing up of being divided into that the jellyfish parent stings, jellyfish kind, and it is as follows to show division:
What 1, the jellyfish parent stung stores up
Jellyfish first sexually matured reach 9 months by the end of August at the beginning of, the jellyfish of gathering the different sea areas of individual weight more than 5kg stings as male parent, maternal parent, by female-male proportion 2-3: 1 goes into the pond, the control water temperature is 20-26 ℃ stores up, and stores up in the phase, changes water every day 2 times, morning 100%, the dusk 50%; Respectively throw something and feed in every morning, afternoon artemia nauplii 1 time, daily ration, feeding quantity with the 1.5-2h that throws something and feeds after in the water body no obvious artemia nauplii distribute and be as the criterion;
2, the jellyfish kind is stung and is laid eggs and hatching
2.1 retaining and water treatment
To obtain ovum pond outwash before growing seedlings, inject fresh filtering sea, 1 meter of the depth of water, every cube of water body adds 4-6g/m 3EDTA, and inflate stand-by;
2.2 lay eggs
Select time is 2:00-3:00 in morning, stops inflation, the parent who cultivates is bitten by male and female obtained in the ovum pond than immigration in 3: 1, and density is controlled at 1-2/m 3Smart back 3h is bitten and lay eggs, arranged to the parent, and it is shifted out from obtain the ovum pond; Treat that Chi Shui leaves standstill 0.5h, upper strata 65cm water siphon is discharged, do not stir Chi Shui during draining, in order to avoid fertilized egg runs off in a large number, and draining will be finished in 1h; Afterwards, add the isothermal filtering sea again to original position, and additional EDTA, little inflation hatching;
2.3 hatching
Under water temperature 20-24 ℃ condition, fertilized egg begins the spilting of an egg through 0.5h, when growing to blastocoele, just floats gradually at the bottom of the pond, and fertilized egg is through 6-8h bud into floating wave larva;
3, the collection of floating wave larva
With floating wave larvae cultivation 20-24h, throw in adherance, this adherance is suspended in the pond, the 30cm at the bottom of the pond of its lower floor, be advisable just to be submerged in the underwater in the upper strata, and injected volume is 3 groups/m 2, stop inflation between the floating wave larva setting stage, add seawater to the water surface every 2-3 days and exceed adherance 25cm; Reach 1-1.5/cm in adherance spatfall density 2The time, begin to change water and trace inflation;
4, the cultivation of hydrula and low temperature are preserved and overwintering culture
4.1 the cultivation of hydrula
After becoming 4 tentacle hydrulas attached to the floating wave larvae development on the adherance, throw something and feed this kind jellyfish floating wave larva or shellfish trochophore, the time of throwing something and feeding begins after 3-4 days for throwing in adherance, throw something and feed every day 1 time, daily ration, feeding quantity be the spatfall amount 5-6 doubly; Grow after 5-6 days to tool 8 tentacle hydrulas in mid-term, the artemia nauplii of throwing something and feeding, bait throwing in per 2 days once, daily ration, feeding quantity with throw something and feed the back 1-2h in no residual bait be as the criterion, and throw something and feed and inflate 2h after change water, quantity of exchanged water is the 1/3-1/2 in full pond; The later stage hydrula for tool 16 tentacles is grown in cultivation through 8-10 days;
4.2 the overwintering culture of hydrula and low temperature are preserved
After hydrula developed into 16 tentacles, the trend of progressively lowering the temperature along with outer seawater was controlled at 3-10 ℃ with the cultivation water temperature of hydrula, and hydrula is carried out overwintering culture and low temperature is preserved; During this period, shut out the light with black plastic film, the control intensity of illumination is no more than 500lux; With the artemia nauplii is that bait is thrown something and fed, and controls daily ration, feeding quantity and the number of times of throwing something and feeding, and throws something and feeds in 1 week 1 time in the time of 3-5 ℃, throws something and feeds weekly in the time of 6-10 ℃ 2 times, and feeding volume is 8-10 a times of hydrula quantity; Changed water once in 5-7 days, quantity of exchanged water is 1/3-1/2, changes before the water dirt at the bottom of the first sucking-off pond, adds the equivalent fresh seawater again;
5, the cultivation of the strobilation of hydrula and ephyra
5.1 the strobilation of hydrula
Next year, the mid or late March water temperature was gone up to more than 10 ℃ the time, remove black plastic film, improve indoor intensity of illumination, the reproduction function of hydrula is recovered gradually, and strengthen daily ration, feeding quantity and the number of times of throwing something and feeding, throw something and feed every day 4 times, no obvious artemia nauplii distributes daily ration, feeding quantity in the water body, hydrula is crocus and is as the criterion after with the 1h that throws something and feeds; Treat that water temperature is raised to about 13 ℃, fall the pond when hydrula transverse fissure begins, and falling behind the pond to heat up 2 ℃ every day, final water temperature maintains 18 ℃, same hydrula repeatedly carries out strobilation during the strobilation, breaks away from parent in succession, produces the ephyra of free swimming;
5.2 the cultivation of ephyra
Under 18-22 ℃ of condition, carry out the cultivation of ephyra, density is cultivated in control, the trace inflation, cultivating through 7-10d becomes umbrella and reaches directly that the 10mm left and right sides is young bites, in the nurturing period, along with the growth of ephyra strengthens the number of times of throwing something and feeding, daily ration, feeding quantity and quantity of exchanged water gradually, artemia nauplii 2-3 time of throwing something and feeding every day, each individual daily ration of feeding 20-300 artemia nauplii; Change water 2-3 every day, and quantity of exchanged water is the 1/2-3/4 in full pond; Cultivating through 7-10d becomes the children that umbrella directly reaches about 20mm and bites again, and reaches the commercial seedling specification, and young biting cultivated the children and bitten, and its food ration heightens, and oxygen consumption and metabolite increase gradually, change water 3-4 time in this every day nurturing period, and quantity of exchanged water is 3/4 of full pond; Throw something and feed every day artemia nauplii 3-4 time, each individual daily ration of feeding 1000-1500 artemia nauplii; And keep being limited to 4mg/L under the water body dissolved oxygen, being limited to 0.15mg/L on the ammoniacal nitrogen, the pH scope is 7.5-8.5.
2, a kind of jellyfish hybrid breeding according to claim 1 is survived the winter and the middle method of breeding, it is characterized in that: the jellyfish in the different sea areas of described collection stings as male parent, maternal parent, its male parent parent stings and is that it is that the wild jellyfish in bay is made tranquil in Shandong that the wild jellyfish of Yancheng, Jiangsu Haizhou Wan, maternal parent sting.
CNA2009100171201A 2009-07-06 2009-07-06 A kind of jellyfish hybrid breeding is survived the winter and the middle method of breeding Pending CN101606499A (en)

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CN103621442A (en) * 2013-11-27 2014-03-12 中国科学院海洋研究所 Indoor totally-artificial jellyfish breeding method
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CN104521844B (en) * 2015-01-20 2016-08-24 大连理工大学 A kind of jellyfish vegetative propagation fine-variety breeding method
CN106417119A (en) * 2016-09-30 2017-02-22 辽宁省海洋水产科学研究院 Method of jellyfish fertilization and hatching
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