CN103651200B - A kind of method for culturing seedlings of Portunus pelagicus - Google Patents

A kind of method for culturing seedlings of Portunus pelagicus Download PDF

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Publication number
CN103651200B
CN103651200B CN201310646623.1A CN201310646623A CN103651200B CN 103651200 B CN103651200 B CN 103651200B CN 201310646623 A CN201310646623 A CN 201310646623A CN 103651200 B CN103651200 B CN 103651200B
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pond
crab
ovigerous
holding
storage pond
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CN103651200A (en
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谭雄亮
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BEIHAI ZHENFA MARINE INDUSTRY SCIENCE & TECHNOLOGY Co Ltd
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BEIHAI ZHENFA MARINE INDUSTRY SCIENCE & TECHNOLOGY Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The present invention relates to the method for culturing seedlings of a kind of Portunus pelagicus, including: parent's crab selection, the preparation of storage pond, holding pond, the management of non-ovigerous crab, the management of ovigerous crab and larval culture, the present invention establishes the method for culturing seedlings of a kind of Portunus pelagicus, carry out the research of Portunus pelagicus artificial breeding technique and increase excellent cultivation new varieties for Guangxi, the breed structure readjustment and marine economy development promoting sea-farming industry is had great importance.

Description

A kind of method for culturing seedlings of Portunus pelagicus
Technical field
The present invention relates to the Cultivating techniques field of swimming crab, the method for culturing seedlings of a kind of Portunus pelagicus.
Background technology
Swimming crab is the seafood product of people's eating, and meat flavour is delicious, nutritious and favored by consumers.I State-owned four big economic crab, i.e. mud crab, Eriocheir sinensis, Portunus trituberculatus Miers, Portunus pelagicus. Portunus trituberculatus Miers fecund China Huanghai Sea, the Bohai Sea, the East Sea, and Portunus pelagicus originates in China South Sea, east more Sea.
Portunus pelagicus (Sea crab), though in west of Guangdong Province Yuexi, that yield is caught in the sea in Guangxi and Hainan is quite big, its money Source is reduced day by day.Portunus pelagicus build is big, adaptable, fast growth, disease are few, economical It is worth high, is a kind of sea-farming kind having much development prospect, is also shrimp pond integrated culture One of improved seeds, good in economic efficiency (per kilogram 20-60 unit).Culture with seawall, the sandy end is Good, utilize the low-quality shrimp pool to carry out culture in a large number, wherein west of Guangdong Province Yuexi, the North Sea and Hainan Region carry out people Work nursery and to form condition the most suitable, best results, but at present, propagate Portunus pelagicus artificially few, The fewest with the technical research data of cultivation about Portunus pelagicus nursery.
Summary of the invention
The present invention is directed to above-mentioned deficiency, disclose the method for culturing seedlings of a kind of Portunus pelagicus:
1) parent crab selects: selecting parent's crab body weight 80~150g, long more than the 7cm of first, appendage is complete;
2) preparation of storage pond, holding pond
20ppmKMnO is used after 2.1 cleanings4Sterilization;
Paving is cleaned in 2.2 storage pond, holding ponds, fine sand 3~5cm after sterilization, accounts for the 50~60% of pond floor space, Described storage pond, holding pond every 4~5 days, thoroughly turn over wash, sterilize fine sand once, prevent sand bed blackening, or fall Pond is once;
2.3 with tile, fragment of brick nests, and for parent, crab is hidden, perch;
2.4 storage pond, holding pond face black cloth shadings, daylight controls 50~100Lux, stone 1 of exitting in pond Individual/m2
3) management of non-ovigerous crab
The close crab of non-oogenesis is put into storage pond, holding pond by 3.1, and the close crab density in described storage pond, holding pond is 1~2 /m2
3.2 storage pond, holding pond water temperatures control at 25~30 DEG C, change water temperature difference and are less than 1~2 DEG C, proportion 1.018~1.025, the depth of water 45~55cm;
Change water 100% 3.3 mornings every day, observe with or without ovigerous crab;
3.4 bait throwing in every day 1 time, dusk or evening throw, and daily ration, feeding quantity is the 5~10% of parent's crab body weight, according to Situation of ingesting adjusts, and shows a small surplus preferably;
4) management of ovigerous crab
After 4.1 find ovigerous crab, it is separated with non-ovigerous crab, puts in other storage pond, holding pond;
The storage pond, holding pond salinity of 4.2 pairs of ovigerous crabs controls 26~30 ‰, and daily amplitude is less than 2 ‰;Temperature 25~30 DEG C, temperature difference per day is less than 1 DEG C, full pool spilling head EDTA5ppm, 2~3 days antimicrobials of splashing, and keeps Inflation;
4.3 monitoring embryonated eggs and embryonic development situation, infect with or without fungi or Zoothamnium, prediction ovigerous crab Hatching date, when ovum colored appearance becomes grey black, in conjunction with microscopy, embryo heartbeat reach 150 times/min with Time upper, ovigerous crab moved into hatchery and hatches;
5) larval culture
5.1 nursery ponds process: scrub with watery hydrochloric acid, and the bleaching powder of 10ppm soaks half an hour, and rinses Totally, then rinsing well with filtering sea after the sterilization of 20ppm potassium permanganate, gas stone is 1.5-2/m2
5.2 nursery ponds management: nursery pond adds water 80~100cm, full pool spilling head EDTA5ppm, Chi Zhong Inoculate a small amount of Micro Algae, concentration 10~12 × 104Cell/ml, water body shows light algae water colour, and the young enters pond First 1 day inoculating rotifer, 5/ml of density, water temperature 25~30 DEG C, proportion 1.015~1.022, pH value 7.8~8.6, Ammonia nitrogen is less than 0.02mg/l, more than dissolved oxygen 5mg/l, illumination 1000~10000Lux;
5.3 young input density: 5 × 104Individual/m3, bait throwing in every day 2~4 times;
5.4, when paedomorphosis to young crab, go out pond. 。
Preferred version is: the described storage pond, holding pond putting non-ovigerous crab every 3~5 days with formalin, Gao Meng Acid potassium or nystatin are to parent's crab dipping.
Preferred version is: bait is according to being respectively Micro Algae, wheel animalcule and artemia the period that the young is different without joint The young.
The present invention establishes the method for culturing seedlings of a kind of Portunus pelagicus, carries out Portunus pelagicus artificial breeding skill Art research, increases the excellent cultivation new varieties breed structure to promoting sea-farming industry for Guangxi Adjust and marine economy development has great importance.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in further detail:
1) parent crab selects: selecting parent's crab body weight 80~150g, long more than the 7cm of first, appendage is complete;
2) preparation of storage pond, holding pond
20ppmKMnO is used after 2.1 cleanings4Sterilization, every 3~5 days with formalin, potassium permanganate Or nystatin is to parent's crab dipping;
Paving is cleaned in 2.2 storage pond, holding ponds, fine sand 3~5cm after sterilization, accounts for the 50~60% of pond floor space, Without sanding near discharge outlet, as feeding area, feeding ground, described storage pond, holding pond every 4~5 days, thoroughly turn over wash, Sterilization fine sand once, prevents sand bed blackening, or pond is once;
2.3 with tile, fragment of brick nests, and for parent, crab is hidden, perch;
2.4 storage pond, holding pond face black cloth shadings, daylight controls 50~100Lux, stone 1 of exitting in pond Individual/m2
3) management of non-ovigerous crab
The close crab of non-oogenesis is put into storage pond, holding pond by 3.1;
3.2 storage pond, holding pond water temperatures control at 25~30 DEG C, best 28 DEG C, change water temperature difference and are less than 1~2 DEG C, Proportion 1.018~1.025, the depth of water 45~55cm;
Changing water 100% 3.3 mornings every day, every day, 8:00 cleared up bait field, removed residual bait, dirt observation With or without ovigerous crab;
3.4 bait throwing in every day 1 time, dusk or evening throw, and daily ration, feeding quantity is the 5~10% of parent's crab body weight, according to Situation of ingesting adjusts, and shows a small surplus preferably, and the density of parent crab is: 1~2/m2
4) management of ovigerous crab
After 4.1 find ovigerous crab, it is separated with non-ovigerous crab, puts in other storage pond, holding pond.Described Storage pond, holding pond every 4~5 days, thoroughly turn over wash, sterilize fine sand once, prevent sand bed blackening, or pond one Secondary, every 3~5 days with formalin, potassium permanganate or nystatin to parent's crab dipping;
The storage pond, holding pond salinity of 4.2 pairs of ovigerous crabs controls 26~30 ‰, and daily amplitude is less than 2 ‰, temperature 25~30 DEG C, temperature difference per day is less than 1 DEG C, full pool spilling head EDTA5ppm, 2~3 days antimicrobials of splashing, and keeps Inflation;
4.3 monitoring embryonated eggs and embryonic development situation, infect with or without fungi or Zoothamnium, prediction ovigerous crab Hatching date, when ovum colored appearance becomes grey black, in conjunction with microscopy, embryo heartbeat reach 150 times/min with Time upper, ovigerous crab moved into hatchery and hatches;
5) larval culture
5.1 nursery ponds process: scrub with watery hydrochloric acid, and the bleaching powder of 10ppm soaks half an hour, and rinses Totally, then rinsing well with filtering sea after the sterilization of 20ppm potassium permanganate, gas stone is 1.5-2/m2
5.2 nursery ponds management: nursery pond adds water 80~100cm, full pool spilling head EDTA5ppm, Chi Zhong Inoculate a small amount of Micro Algae, concentration 10~12 × 104Cell/ml, water body shows light algae water colour, and the young enters pond First 1 day inoculating rotifer, 5/ml of density, water temperature 25~30 DEG C, proportion 1.015~1.022, pH value 7.8~8.6, Ammonia nitrogen is less than 0.02mg/l, more than dissolved oxygen 5mg/l, illumination 1000~10000Lux;
5.3 young input density: 5 × 104Individual/m3, bait throwing in every day 2~4 times, bait is different according to the young Period be respectively Micro Algae, wheel animalcule and artemia nauplii;
5.4, when paedomorphosis to young crab, go out pond;
6) food organisms are cultivated
6.1 Micro Algaes are cultivated
Nutrient solution is filled a prescription: sodium nitrate 60ppm, potassium dihydrogen phosphate 5ppm, ironic citrate 1ppm.Algae kind It is 1: 2-5 that level cultivates the ratio of the algae kind and the new nutrient solution that access, and inoculation time is typically chosen in the morning 8: Carrying out between 00-10:00, Micro Algae is at normal temperatures through the cultivation of 10d, and concentration reaches 4 × 105Individual/ml Above, now as bait feeding.
6.2 wheel animalcules are cultivated
The bait of wheel animalcule is based on Micro Algae and yeast.When illumination condition is preferable, first add in culture pond Enter nutritive salt, to cultivate Micro Algae, after the water in container slightly has color, inoculate wheel animalcule.Connecing of wheel animalcule Plant density to be typically advisable with 100-200/ml.When Micro Algae can not meet the nutritional need of wheel animalcule, it is necessary to Based on yeast of throwing something and feeding, with 250 mesh silk cover filterings before throwing something and feeding, day feeding volume be 0.8~1.2g/ (d 106Wheel Worm), throw something and feed for twice.Under the conditions of suitable temperature, bait, dissolved oxygen, illumination etc., through 8~10d trainings Educate, expand cultivation or results when the density of wheel animalcule reaches 200/more than ml.Micro Algae is used before results Strengthening uses two days later.The special mesh bag made with 200 mesh sieve thin,tough silk filters results.Wheel animalcule can not meet life When product needs, buy from Zhanjiang and Fujian.
When wheel animalcule density is relatively low, and when daily ration, feeding quantity is less, generality is inflated, when wheel animalcule density is higher by (500 Individual/ml), when daily ration, feeding quantity is bigger, it is necessary to strength inflation, to ensure dissolved oxygen certain in water body.Daily ration, feeding quantity is 0.8~1.2g/ (d 106Wheel animalcule) it is ideal.Total principle is: when wheel animalcule density is low in early days, bait Material to be thrown, and when wheel animalcule density is higher, bait is thrown less.Wheel animalcule density is low in early days Time, throw 1~2 every day;And later stage wheel animalcule density is high, throw 2~5 every day.
Wheel animalcule culture pond, through the cultivation of 7d, is necessary for carrying out down pond, to remove what residual bait corruption caused It is unfavorable for the factor that wheel animalcule is cultivated.When High Density Cultivation all the more so.In order to ensure that High Density Cultivation is taken turns Worm can be normally carried out, and preferably ensures that 2~3d fall 1 pond.So can in wheel animalcule filtration and transfer process Can lose a part of wheel animalcule, but can guarantee that the purpose reaching short time High Density Cultivation.

Claims (3)

1. the method for culturing seedlings of a Portunus pelagicus, it is characterised in that step includes:
1) parent crab selects: selecting parent's crab body weight 80~150g, long more than the 7cm of first, appendage is complete;
2) preparation of storage pond, holding pond
20ppmKMnO is used after 2.1 cleanings4Sterilization;
Paving is cleaned in 2.2 storage pond, holding ponds, fine sand 3~5cm after sterilization, accounts for the 50~60% of pond floor space, institute The storage pond, holding pond stated every 4~5 days, thoroughly turn over wash, sterilize fine sand once, prevent sand bed blackening, or pond Once;
2.3 with tile, fragment of brick nests, and for parent, crab is hidden, perch;
2.4 storage pond, holding pond face black cloth shadings, daylight controls 50~100Lux, and exit in pond 1/m of stone2
3) management of non-ovigerous crab
The close crab of non-oogenesis is put into storage pond, holding pond by 3.1, and the close crab density in described storage pond, holding pond is 1~2/m2
3.2 storage pond, holding pond water temperatures control at 25~30 DEG C, change water temperature difference and are less than 1~2 DEG C, proportion 1.018~1.025, The depth of water 45~55cm;Change water 100% 3.3 mornings every day, observe with or without ovigerous crab;
3.4 bait throwing in every day 1 time, dusk or evening throw, and daily ration, feeding quantity is the 5~10% of parent's crab body weight, according to ingesting Situation adjusts, and shows a small surplus preferably;
4) management of ovigerous crab
After 4.1 find ovigerous crab, it is separated with non-ovigerous crab, puts in other storage pond, holding pond;
The storage pond, holding pond salinity of 4.2 pairs of ovigerous crabs controls 26~30 ‰, and daily amplitude is less than 2 ‰;Temperature 25~30 DEG C, Temperature difference per day is less than 1 DEG C, full pool spilling head EDTA5ppm, 2~3 days antimicrobials of splashing, and keeps inflation;
4.3 monitoring embryonated eggs and embryonic development situation, infect with or without fungi or Zoothamnium, prediction ovigerous crab Hatching date, when ovum colored appearance becomes grey black, in conjunction with microscopy, embryo heartbeat reaches 150 times/more than min Time, ovigerous crab is moved into hatchery and hatches;
5) larval culture
5.1 nursery ponds process: scrub with watery hydrochloric acid, and the bleaching powder of 10ppm soaks half an hour, and rinses well, Rinsing well with filtering sea after sterilizing with 20ppm potassium permanganate, gas stone is 1.5-2/m again2
5.2 nursery ponds management: nursery pond adds water 80~100cm, full pool spilling head EDTA5ppm, inoculates in pond A small amount of Micro Algae, concentration 10~12 × 104Cell/ml, water body shows light algae water colour, and the young enters before pond 1 It inoculating rotifer, 5/ml of density, water temperature 25~30 DEG C, proportion 1.015~1.022, pH value 7.8~8.6, Ammonia nitrogen is less than 0.02mg/l, more than dissolved oxygen 5mg/l, illumination 1000~10000Lux;
5.3 young input density: 5 × 104Individual/m3, bait throwing in every day 2~4 times;
5.4, when paedomorphosis to young crab, go out pond.
The method for culturing seedlings of Portunus pelagicus the most according to claim 1, it is characterised in that put non-ovigerous crab Storage pond, holding pond every 3~5 days with formalin, potassium permanganate or nystatin to parent's crab dipping.
The method for culturing seedlings of Portunus pelagicus the most according to claim 1, it is characterised in that bait is according to children Body is respectively Micro Algae, wheel animalcule and artemia nauplii at different period.
CN201310646623.1A 2013-12-04 2013-12-04 A kind of method for culturing seedlings of Portunus pelagicus Expired - Fee Related CN103651200B (en)

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CN105028261B (en) * 2015-06-18 2017-09-29 中国水产科学研究院东海水产研究所 One kind preventing and treating mycotic method of Scylla paramamosain seed
CN105145424A (en) * 2015-08-04 2015-12-16 厦门大学 Culturing method for blue crab egg-carrying crab phase
CN106035164B (en) * 2016-06-16 2018-11-09 中国水产科学研究院下营增殖实验站 A kind of method that northern area improves Portunus trituberculatus Miers seed rearing survival rate
CN109997754A (en) * 2019-05-15 2019-07-12 湖南助农农业科技发展有限公司 A kind of cultural method of lobster volume increase

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CN100455188C (en) * 2006-12-21 2009-01-28 张玉满 Outdoor earth pond ecological culturing of portunid juvenile crabs
CN102106278A (en) * 2010-12-30 2011-06-29 广东海洋大学 Method for breeding marine crabs artificially
CN102119675A (en) * 2010-12-30 2011-07-13 广东海洋大学 Method for cultivating portunus pelagicus

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