CN85103042A - Method of culturing marine chlorella - Google Patents
Method of culturing marine chlorella Download PDFInfo
- Publication number
- CN85103042A CN85103042A CN 85103042 CN85103042A CN85103042A CN 85103042 A CN85103042 A CN 85103042A CN 85103042 CN85103042 CN 85103042 CN 85103042 A CN85103042 A CN 85103042A CN 85103042 A CN85103042 A CN 85103042A
- Authority
- CN
- China
- Prior art keywords
- medium
- acetate
- carbon source
- addition
- green alga
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Method of culturing marine chlorella is that to utilize seawater be that medium is cultivated the marine products green alga, it is characterized by, and in this medium, adds to be selected from and at least aly in bicarbonate, acetate and the acetate is carbon source, and add vitamin h (biotin) to carry out culturist.
Description
The present invention system is about method of culturing marine chlorella, and You Zhike improves eicosapentenoic acid content, improving in the marine products green alga of being cultivated fat valency content always, and the marine products green alga cultural method that can quicken the speed of giving birth to.
Eicosapentenoic acid (Eicosapentanoic acid is to call EPA in the following text) is the presoma of various prostaglandins (Prostaglandin) hazardous substance in the organism, has physiologically actives such as anti-platelet aggregation in recent years because of discovery, quite attracts attention.In the past, though EPA can be by the fish oil separation and purification, for the TL in the fish oil, EPA content is low to moderate about 10-15%, and in fish oil, in the high-purity unsaturated fatty acid beyond the EPA, contain the similar fatty acid of relatively large and EPA structure, be not easy further to be concentrated.
Aspect in addition, when the marine products green alga is cultivated under certain condition, its EPA content can reach be equivalent to the total fatty acids amount about 30% or more than, be height than fish oil.Yet the fertility speed of marine products green alga is slow, and total fatty acid content is low, so absolute quantity is also low, is its problem place.
Therefore, the present invention's purpose is to provide a kind of method of culturing marine chlorella, can improve TL amount (thereby can improve EPA content) and can improve fertility speed.
According to marine products green alga cultural method provided by the present invention, be that to utilize seawater be medium culture marine products green algas, it is characterized by, in this medium, add being selected from bicarbonate, in acetate and the acetate, at least a is carbon source, and adds in addition culturist of vitamin h.
Now be described in further detail the present invention.
As mentioned above, method of culturing marine chlorella of the present invention is characterized in that the medium of employing, and lying in seawater is to add specific carbon source and vitamin h in the medium.
Used minimal medium, all natural sea-waters or artificial seawater (general name " sea water medium ") are all available.Yet the sodium chloride concentration in medium is good (when using natural sea-water, but mat adds water etc. to regulate its concentration) with 0.1-0.2 weight %.When sodium chloride content exceeded this scope, fertility speed can significantly descend, the subsidiary EPA content that reduces.
According to the present invention, be the carbon source that medium adds at above-mentioned seawater, bicarbonate is arranged, acetate, acetate, or its wantonly mixture more than two kinds.The addition of this carbon source is generally about 30 to 1000 milligrams of every liter of medium.This addition is less than 30 milligrams of/liter medium, and then the result can't improve the total lipid content of the green alga of cultivating, aspect in addition, and how addition is as surpassing 1000 milligrams of/liter medium, can shows that effect has special raising.The preferable addition of carbon source is 100-600 milligram/litre medium.The example of sodium bicarbonate has sodium bicarbonate, and saleratus etc., acetate then have sodium acetate, potassium acetate, ammonium acetate etc.
According to the present invention, make an addition to the vitamin h that seawater is a medium (biotin) simultaneously with above-mentioned carbon source, it measures not specially provided for, only in 0.1 microgram/when the litre medium is following, several days effects.The preferable addition of vitamin h is the 0.5-10 microgram.
Except that above-mentioned carbon source and vitamin h, seawater is that medium still can add nitrogenous sources such as ammonium sulfate, phosphorus sources such as peracid calcium.Nitrogenous source is with the ratio of about 100 milligrams of/liter medium, and the phosphorus source is added to good with the ratio of about 10 milligrams of/liter medium.
The pH value of medium can be with incubation time through changing, but the medium of (be the inoculation of marine products green alga before) before the marine products green alga is cultivated beginning at least, is good to be set in 6 to 8 pH value.
In the present invention, when adopting above-mentioned medium, marine products green alga (for example chlorellaminutissima) is preferably in 15 ℃ to 25 ℃ temperature and cultivates.When cultivation temperature exceeded this scope, fertility speed descended, and EPA content has the anxiety of decline in the green alga.Cultivation is generally 3-20 days between planting.
When cultivating the marine products green alga according to the present invention, the green alga inoculum concentration does not have special restriction, normally inoculates with the ratio of 0-0.5 gram (wet thallus weight)/litre medium.
According to marine products green alga cultural method of the present invention, can accelerate fertility speed, keep shared EPA content height in the TL, stabilized the marine products green alga that produces the total lipid content height, this be selected from bicarbonate, at least a carbon source of selecting among acetate and the acetate group, and the effect that multiplies each other of vitamin h, according to inference is that fatty acid generates in the first step, promote the third two anilide COA to generate so.
Again, utilize the carbon source supply method of photosynthesis, known have the method that carbonic acid gas is sneaked in sudden and violent gas, but according to the present invention, compare with prior art method, and utilizing of carbon source is preferable, can reduce production costs.
After cultivating the marine products green alga according to the present invention, will cultivate the green alga thalline with appropriate solvent, for example the mixed extractant solvent of hexane and isopropyl alcohol separates lipid components, utilizes the well-established law halogenation to decompose.After removing not halogenation composition, collect fatty acid, saturated with urea additives method, and remove unsaturated mono fatty acid.Gained fatty acid like this, its EPA content is quite high, improves purity again as desiring, and then can utilize column type chromatographies such as silica gel.The column type chromatography is separated by this, can get 95% above purity.
Now it is as follows to record and narrate example of the present invention.
Embodiment
Carbon source shown in the table 1 and vitamin h, with ratio shown in this table (/ litre medium), making an addition to seawater is that basic culture solution (to call BM in the following text) is modulated into medium, pH regulator to 7.4 respectively.The composition of basic culture solution is as shown in table 2.Each medium of so being ready for., cultivated 4 days down in sudden and violent gas at 20 ℃ with the fluorescent lamp Continuous irradiation inoculation marine products green algas (0.1 gram/litre medium) back.After cultivation is finished, utilize centrifugal separation to collect the thalline freeze drying after, the extraction lipid is analyzed fatty acid and is formed and divide.The result is as shown in table 3.Again, fertility speed is cell concentration (wet thallus weight/litre medium) expression when finishing with cultivation.
Table 1 (medium is formed and divided)
By result shown in the table 3 as can be known, the inventive method (medium 4-6) is compared with additive method (medium 1-3), determines that fertility speeds up, and total lipid content increases.Though this result does not change EPA content,, can significantly improve total receipts amount of EPA according to the present invention.
Claims (3)
1, method of culturing marine chlorella is that to utilize seawater be medium culture marine products green algas, it is characterized by, and in this medium, adds being selected from sodium bicarbonate, at least a carbon source in acetate and the sodium acetate, and vitamin h, in addition culturist.
2, as the cultural method of the 1st of request patent part, wherein, the carbon source addition is every liter of medium 30-1000 milligram person.
3, as request patent part the 1st or 2 s' cultural method, wherein, the vitamin h addition is every liter of medium 0.5-10 microgram person.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85103042 CN85103042B (en) | 1985-04-20 | 1985-04-20 | Method of culturing marine chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85103042 CN85103042B (en) | 1985-04-20 | 1985-04-20 | Method of culturing marine chlorella |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN85103042A true CN85103042A (en) | 1986-10-15 |
| CN85103042B CN85103042B (en) | 1987-01-14 |
Family
ID=4792941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 85103042 Expired CN85103042B (en) | 1985-04-20 | 1985-04-20 | Method of culturing marine chlorella |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN85103042B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930949B (en) * | 2005-09-16 | 2010-06-09 | 中国科学院大连化学物理研究所 | Marine green algae cultivating process |
| CN101822271A (en) * | 2010-05-11 | 2010-09-08 | 宁波大学 | Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof |
| CN110122312A (en) * | 2019-03-11 | 2019-08-16 | 华南理工大学 | A method of improving sargassum fusifome growth of seedling |
-
1985
- 1985-04-20 CN CN 85103042 patent/CN85103042B/en not_active Expired
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930949B (en) * | 2005-09-16 | 2010-06-09 | 中国科学院大连化学物理研究所 | Marine green algae cultivating process |
| CN101822271A (en) * | 2010-05-11 | 2010-09-08 | 宁波大学 | Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof |
| CN110122312A (en) * | 2019-03-11 | 2019-08-16 | 华南理工大学 | A method of improving sargassum fusifome growth of seedling |
| CN110122312B (en) * | 2019-03-11 | 2021-05-14 | 华南理工大学 | Method for improving growth of seedlings of sargassum fusiforme |
Also Published As
| Publication number | Publication date |
|---|---|
| CN85103042B (en) | 1987-01-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101519676B (en) | Method for producing docosahexenoic acid by fermenting schizochytrium | |
| Gibor | The culture of brine algae | |
| Barghbani et al. | Investigating the effects of several parameters on the growth of Chlorella vulgaris using Taguchi's experimental approach | |
| CN101812484B (en) | Method for producing DHA by Schizochytrium in high-density culture through fermentation | |
| Yongmanltchal et al. | Growth and eicosapentaenoic acid production by Phaeodactylum tricornutum in batch and continuous culture systems | |
| ATE374531T1 (en) | ENHANCED PRODUCTION OF LIPIDS CONTAINING POLYUNSATURATED FATTY ACIDS BY HIGH DENSITY CULTURES OF EUCARIOTIC MICROBE IN FERMENTATION DEVICES | |
| Grant Burgess et al. | An optical fibre photobioreactor for enhanced production of the marine unicellular alga Isochrysis aff. galbana T-Iso (UTEX LB 2307) rich in docosahexaenoic acid | |
| US6410282B1 (en) | Method for enhancing levels of polyunsaturated fatty acids in thraustochytrid fungi | |
| Emelyanova | Lipid and γ-linolenic acid production by Mucor inaquisporus | |
| CN1218035C (en) | Culture of long chain unsaturated fatty acid by heterotrophic marine microalgal | |
| US4485172A (en) | Multistage process for the preparation of fats and oils | |
| US4485173A (en) | Preparation of fats and oils | |
| CN85103042A (en) | Method of culturing marine chlorella | |
| EA011877B1 (en) | Process for the cultivation of microorganisms of the genus thraustochytriales | |
| Spectorova et al. | High-density culture of marine microalgae—promising items for mariculture: I. mineral feeding regime and installations for culturing Dunaliella tertiolecta Butch | |
| CN1710060A (en) | Traingular brown algae open culture method and its special culture meidum | |
| JPS57144986A (en) | Preparation of lipid having high content of gamma-linolenic acid | |
| KR20060019507A (en) | A method of enhancing levels of polyunsaturated fatty acids in thraustochytrid protists | |
| McLachlan | The growth of unicellular algae in artificial and enriched sea water media | |
| RU92008481A (en) | METHOD OF OBTAINING BIOMASS METHANE OXIDIZING MICROORGANISMS | |
| JP5371750B2 (en) | Method for producing DHA-containing phospholipids by microbial fermentation | |
| JPS63219387A (en) | Production of optically active cis-cyclopentene-3,5-diol monoester | |
| SU1324627A1 (en) | Strain of algae dunaliella salina teod calv-834 - producer of protein-carotene biomass | |
| KR20020092156A (en) | A method for enhancing levels of polyunsaturated fatty acids in thraustochytrid fungi | |
| RU93056583A (en) | METHOD FOR PRODUCING BIOMASS ENRICHED WITH SELENIUM |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| PB01 | Publication | ||
| C06 | Publication | ||
| C13 | Decision | ||
| GR02 | Examined patent application | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |