CN106508166A - Method for breaking dormancy of kmeria septentrionalis seeds - Google Patents
Method for breaking dormancy of kmeria septentrionalis seeds Download PDFInfo
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- CN106508166A CN106508166A CN201610838002.7A CN201610838002A CN106508166A CN 106508166 A CN106508166 A CN 106508166A CN 201610838002 A CN201610838002 A CN 201610838002A CN 106508166 A CN106508166 A CN 106508166A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The present invention discloses a method for breaking dormancy of kmeria septentrionalis seeds, and the method includes the following steps: seed disinfection: disinfecting kmeria septentrionalis seeds; gibberellin and stratification treatment: soaking disinfected kmeria septentrionalis seeds in a GA3 solution with a concentration of 0.5-2 mmol/L for 12-24 hour, then mixing with wet perlite at a volume ratio of 1:3, storing in a plastic valve bag, sealing the bag and putting in a 4 DEG C constant-temperature incubator for stratification for 90-100 days; germination accelerating: sowing the seeds subjected to stratification in a germination box, employing wet perlite as a matrix, putting the germination box in a seed germination case, controlling the temperature at 30 DEG C for 12 hours under a light condition, then controlling the temperature at 20 DEG C for 12 hours under a dark condition, alternating the above two operations for 28 days and then stopping accelerating germination. The method can break the dormancy of kmeria septentrionalis seeds, shorten the time for germination, and effectively improve the germination percentage of kmeria septentrionalis seeds.
Description
Technical field
The present invention relates to the seed dormant side of Kmeria septentrionalis is broken in nursery stock production raising technology field, specifically one kind
Method.
Background technology
Evergreen Broad-leaved Tree Species of the Kmeria septentrionalis for Magnoliaceae Kmeria, its tree body are tall and big, tree-like beautiful, spend pure white virtue
Perfume (or spice), material are excellent, are precious commerical tree species and flower garden afforestation tree.Kmeria septentrionalis is only distributed in the Libo County Mao Lan states in Guizhou
The wood in family level nature reserve area and Guangxi discusses Nature Reserve, and distribution in flakes is detected in the domestic Guangxi wood in ring river and discusses state
In family's level nature reserve area, area about 3hm2, existing 200 plants of the tree that grows into forest.Under natural conditions, Kmeria septentrionalis germination percentage is extremely low, from
So updating ability is poor, is listed in Chinese one-level national key protected plant.
Kmeria septentrionalis seed just starts to sprout in after planting 100 days or so, and completing germination process needs more than 80 days.Temperature
Though water, chemicals treatment and husky Tibetan etc. are processed can improve Kmeria septentrionalis percentage of seedgermination, effect on driving birds is not good, highest germination rate only 60%
Left and right, fails fully to break Kmeria septentrionalis seed dormancy, shortens which and sprouts required time.Therefore, need one kind badly effectively to beat
The seed dormant method of broken Kmeria septentrionalis, is that Kmeria septentrionalis artificial breeding and large-scale planting provide sound assurance.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least will be described later excellent
Point.
It is a still further object of the present invention to provide one kind breaks the seed dormant method of Kmeria septentrionalis.Which passes through seed and disappears
Poison, gibberellin and Stratificated treatment, vernalization break Kmeria septentrionalis seed dormancy, shorten the time required to sprouting, and effectively improve unisexuality wood
Blue percentage of seedgermination, while be also of very high actual application value in scientific research.
In order to realize these purposes of the invention and further advantage, there is provided one kind breaks Kmeria septentrionalis seed dormancy
Method, comprise the following steps：
S1, seed disinfection：Kmeria septentrionalis seed is carried out disinfection；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 0.5～2mmol/L3Solution
In, the seed after immersion is taken out after 12～24h, then with wet perlite with 1:3 volume ratio mixing, loads plastics valve bag
In, lamination 90～100 days in 4 DEG C of constant incubators are placed in after sealing；
S3, vernalization：Seed after lamination is seeded in germination box, with wet perlite as matrix, then germination box is put
Enter in seed germination box, seed germination box is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately
Carry out, after 28 days, terminate vernalization.
Preferably, need before seed disinfection in S1 through collecting seed and post-processing：In 9～October gather natural cracking into
Ripe Kmeria septentrionalis fruit, is stacked at the shady and cool interior of ventilation, picks seed, be placed in clear water and be dipped to after fruit fully ftractures
After planting soft and soggyization, removal outer layer redness aril is washed by rubbing with the hands.
Preferably, in S1, seed disinfection method is：By the liquor potassic permanganate immersion that seed mass fraction is 0.1%
2h。
Preferably, in S2 valve bag bottom equipped with sodium peroxide as oxygenating agent, oxygenating agent volume account for seed with it is wet
The 1%～1.5% of perlite cumulative volume.
Preferably, in S2 during earthing of seeds, the ventilative 30～40min of sack was opened per 7 days, and is kept the skin wet.
Preferably, in S2 and S3, wet perlite preparation method is：By 2～3 weight portion of ethanol, 0.1～0.2 weight of propane diols
Amount part, 0.01～0.03 weight portion of hydroquinones, 0.2～0.3 weight portion of alanine, 0.1～0.3 weight portion of glutamic acid, apple
Sour 0.1～0.3 weight portion, 0.1～0.3 weight portion of butanedioic acid are dissolved in the distilled water of 300～500 weight portions, then lead to thereto
Enter ozone gas and soak is obtained, ozone concentration reaches 8mg/L in soak, perlite is soaked 3～5h in soak, i.e.,
Obtain wet perlite.
Preferably, the seed in S3 after lamination is needed through following process before being placed in germination box：To the seed after lamination
Surface sprays mixed liquor, then carries out the irradiation of 20～25min using uviol lamp to the surface of the seed, reuses infrared lamp to seed
Surface carries out the irradiation of 10～15min, persistently stirs seed twice in irradiation process；It is described mixing liquid and preparation method thereof be：To 100
Ozone gas being continually fed in weight portion distilled water Ozone Water being obtained, ozone Measuring Ozone Concentration in Water is 5mg/L, is subsequently adding 2～3
The sodium carbonate of weight portion, 1～2 weight portion sodium acid carbonate, the talcum powder for adding 1～2 weight portion after dissolving stir, that is, make
Obtain mixed liquor.
Preferably, it is 15 to be also added with S3 mesostromas with wet perlite volume ratio:1～1.2 dodder extract；
Dodder extract preparation method is：The fresh seed of Chinese dodder with root is cleaned, it is drained, be cut into 0.8～1cm into segment after
50g is weighed, is then fully mixed with 500mL absolute ethyl alcohols, 100mL distilled water, use quality fraction is that 0.5% pyrolkigneous liquid will
PH is adjusted to 4.5～6.5,10～20min of ultrasound under 60～70KHz frequencies, then stands 10～12h, then at 30～40KHz
20～30min of ultrasound under frequency, then stand 24～36h, then with 4 layers of filtered through gauze 2 times, then will filter gained filtrate move into from
Heart pipe, 3500r/min centrifugation 10min, takes supernatant after centrifugation, absolute ethyl alcohol, then plus 500mL distillations is evaporated off in 80 DEG C of backspins
Water dilutes, and obtains dodder extract.
Preferably, seed germination box is opened every 36～48h in S3 pregermination procedures in germination box spray a Tu silk
Sub- leaching liquor.
Preferably, in S2 and S3, wet perlitic absolute water content is maintained at 200%～300%.
The present invention at least includes following beneficial effect：
Firstth, the Kmeria septentrionalis seed after sterilization is soaked in into the GA that concentration is 0.5～2mmol/L3In solution, 12～
The seed after immersion is taken out after 24h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 90～100 days in 4 DEG C of constant incubators, lamination is carried out to Kmeria septentrionalis seed by gibberellin and wet perlite
Process, intraseminal inhibiting substances ABA content can be reduced, increase the content of seed inner gibberellin and the basic element of cell division, effectively
Break the dormancy of Kmeria septentrionalis seed；
Secondth, in S2, the bottom of valve bag is equipped with sodium peroxide as oxygenating agent, and oxygenating agent volume accounts for seed with wet pearl
The 1%～1.5% of rock cumulative volume, seed can absorb O due to respiration2Discharge CO2, cause O in valve bag2Content get over
Come lower, sodium peroxide and CO2React and can discharge O2, reach O in supplementary valve bag2Effect, exhaling for seed can be promoted
Inhale, accelerate the speed of breaking dormancy；
3rd, ethanol, propane diols, hydroquinones, alanine, glutamic acid, malic acid, butanedioic acid are dissolved in into distilled water, so
It is passed through ozone gas afterwards thereto and soak is obtained, ozone concentration reaches 8mg/L in soak, and perlite is soaked in soak
3～5h of bubble, is obtained wet perlite, can effectively kill the harmful levels of pathogens on perlite surface, prevents Kmeria septentrionalis seed from occurring to rot
It is rotten；
4th, the surface of the seed after lamination is sprayed containing ozone, sodium carbonate, sodium acid carbonate and talcous mixed liquor,
Then the irradiation of 20～25min is carried out using uviol lamp to the surface of the seed, reuse infrared lamp carry out 10 to the surface of the seed～
The irradiation of 15min, persistently stirs seed twice in irradiation process, talcum powder can be by ozone, sodium carbonate and bicarbonate in mixed liquor
Sodium effectively absorption, on its surface, is sprayed after the surface of the seed, and the surface of the seed occurs one layer of talcum powder film, then with uviol lamp,
Infrared light irradiation, will not hurt seed, moreover it is possible to effectively kill the harmful levels of pathogens of the surface of the seed after lamination, while promoting in seed
Gibberellin and Cytokinin Contents further increase；
5th, the fresh seed of Chinese dodder with root is cleaned, it is drained, fully mixed with absolute ethyl alcohol, distilled water, using matter
Amount fraction is that 0.5% pyrolkigneous liquid adjusts pH to 4.5～6.5, then ultrasound, standing, filtration, centrifugation, takes supernatant revolving and removes
Absolute ethyl alcohol, then plus distilled water diluting, obtain dodder extract, in acid condition the seed of Chinese dodder extracted, can be by Tu
The materials such as the glucoside in silk are extracted, and after dodder extract is mixed with wet pearlite interstitial substance, can promote Kmeria septentrionalis seed
Sprouting, effectively improve the germination percentage of seed.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention is elaborated with reference to embodiment, to make those of ordinary skill in the art refering to after this specification
Can implement according to this.
<Embodiment 1>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：Kmeria septentrionalis seed is carried out disinfection；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 0.5mmol/L3In solution,
The seed after immersion is taken out after 12h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 90 days in 4 DEG C of constant incubators；
S3, vernalization：By the seed after lamination be seeded in germination box in, with wet perlite as matrix, be added with matrix with
Wet perlite volume ratio is 15:1 dodder extract, is then put into germination box in seed germination box, and seed germination box sets
It is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately, terminate vernalization after 28 days.
<Embodiment 2>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：Kmeria septentrionalis seed is carried out disinfection；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 2mmol/L3In solution, 24h
Afterwards the seed after immersion is taken out, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, after sealing
It is placed in lamination 100 days in 4 DEG C of constant incubators；
S3, vernalization：By the seed after lamination be seeded in germination box in, with wet perlite as matrix, be added with matrix with
Wet perlite volume ratio is 15:1.2 dodder extract, is then put into germination box in seed germination box, seed germination box
It is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately, terminate vernalization after 28 days.
<Embodiment 3>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：Kmeria septentrionalis seed is carried out disinfection；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 1.2mmol/L3In solution,
The seed after immersion is taken out after 18h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 95 days in 4 DEG C of constant incubators；
S3, vernalization：By the seed after lamination be seeded in germination box in, with wet perlite as matrix, be added with matrix with
Wet perlite volume ratio is 15:1.1 dodder extract, is then put into germination box in seed germination box, seed germination box
It is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately, terminate vernalization after 28 days.
<Embodiment 4>
The invention provides breaking the seed dormant method of Kmeria septentrionalis, comprise the following steps：
S1, seed disinfection：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in September part, the shady and cool room of ventilation is stacked at
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin, obtains Kmeria septentrionalis seed, and Kmeria septentrionalis seed is disappeared with the liquor potassic permanganate immersion 2h that mass fraction is 0.1%
Poison；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 0.5mmol/L3In solution,
The seed after immersion is taken out after 12h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 90 days in 4 DEG C of constant incubators, lamination process opened the ventilative 30min of sack per 7 days, and kept the skin wet；
S3, vernalization：Seed after lamination is seeded in germination box, with wet perlite as matrix, then germination box is put
Enter in seed germination box, seed germination box is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately
Carry out, after 28 days, terminate vernalization；
Wherein, in S2 and S3, wet perlitic absolute water content is maintained at 200%.
<Embodiment 5>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in October, is stacked at the shady and cool room of ventilation
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin, obtains Kmeria septentrionalis seed, and Kmeria septentrionalis seed is disappeared with the liquor potassic permanganate immersion 2h that mass fraction is 0.1%
Poison；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 2mmol/L3In solution, 24h
Afterwards the seed after immersion is taken out, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, after sealing
Lamination 100 days in 4 DEG C of constant incubators are placed in, lamination process opened the ventilative 40min of sack per 7 days, and kept the skin wet；
S3, vernalization：Seed after lamination is seeded in germination box, with wet perlite as matrix, then germination box is put
Enter in seed germination box, seed germination box is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately
Carry out, after 28 days, terminate vernalization；
Wherein, in S2 and S3, wet perlitic absolute water content is maintained at 300%.
<Embodiment 6>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in September part, the shady and cool room of ventilation is stacked at
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin, obtains Kmeria septentrionalis seed, and Kmeria septentrionalis seed is disappeared with the liquor potassic permanganate immersion 2h that mass fraction is 0.1%
Poison；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 1.2mmol/L3In solution,
The seed after immersion is taken out after 18h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 95 days in 4 DEG C of constant incubators, lamination process opened the ventilative 35min of sack per 7 days, and kept the skin wet；
S3, vernalization：Seed after lamination is seeded in germination box, with wet perlite as matrix, then germination box is put
Enter in seed germination box, seed germination box is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately
Carry out, after 28 days, terminate vernalization；
Wherein, in S2 and S3, wet perlitic absolute water content is maintained at 250%.
<Embodiment 7>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in September part, the shady and cool room of ventilation is stacked at
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin, obtains Kmeria septentrionalis seed, and Kmeria septentrionalis seed is disappeared with the liquor potassic permanganate immersion 2h that mass fraction is 0.1%
Poison；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 0.5mmol/L3In solution,
The seed after immersion is taken out after 12h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 90 days in 4 DEG C of constant incubators, lamination process opened the ventilative 30min of sack per 7 days, and kept the skin wet；It is described
Equipped with sodium peroxide as oxygenating agent, oxygenating agent volume accounts for the 1% of seed and wet perlite cumulative volume for the bottom of valve bag；
S3, vernalization：The surface of the seed after lamination is sprayed into mixed liquor, then the surface of the seed is carried out using uviol lamp
The irradiation of 20min, reuse infrared lamp carries out the irradiation of 10min to the surface of the seed, persistently stirs seed twice in irradiation process；
Again the seed after process is seeded in germination box, with wet perlite as matrix, is added with matrix and wet perlite volume ratio
For 15:1 dodder extract, is then put into germination box in seed germination box, and seed germination box is set to：30 DEG C of illumination bars
12h under part, 12h under 20 DEG C of dark conditions, so alternately, open seed germination box every 36h and spray one in germination box
Secondary dodder extract, terminated vernalization after 28 days；It is described mixing liquid and preparation method thereof be：Persistently lead to in 100 weight portion distilled water
Enter ozone gas and Ozone Water is obtained, ozone Measuring Ozone Concentration in Water is 5mg/L, is subsequently adding sodium carbonate, 1 weight portion of 2 weight portions
Sodium acid carbonate, the talcum powder for adding 1 weight portion after dissolving stir, that is, mixed liquor is obtained；The dodder extract system
As method it is：The fresh seed of Chinese dodder with root is cleaned, it is drained, be cut into 0.8cm into segment after weigh 50g, Ran Houyu
500mL absolute ethyl alcohols, 100mL distilled water are fully mixed, and use quality fraction is that pH is adjusted to 4.5 by 0.5% pyrolkigneous liquid, then at
Under 60KHz frequencies, ultrasound 10min, then stands 10h, the ultrasound 20min under 30KHz frequencies, then stands 24h, then with 4 layers
Filtered through gauze 2 times, then gained filtrate immigration centrifuge tube will be filtered, 3500r/min centrifugation 10min take supernatant, in 80 after centrifugation
DEG C backspin is evaporated off absolute ethyl alcohol, then plus 500mL distilled water dilutings, obtain dodder extract；
Wherein, in S2 and S3, wet perlitic absolute water content is maintained at 250%；Wet perlite preparation method is：By second
2 weight portion of alcohol, 0.1 weight portion of propane diols, 0.01 weight portion of hydroquinones, 0.2 weight portion of alanine, 0.1 weight portion of glutamic acid,
0.1 weight portion of malic acid, 0.1 weight portion of butanedioic acid are dissolved in the distilled water of 300 weight portions, are then passed through ozone gas system thereto
Soak is obtained, ozone concentration reaches 8mg/L in soak, perlite is soaked 3h in soak, that is, obtains wet perlite.
<Embodiment 8>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in October, is stacked at the shady and cool room of ventilation
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin, obtains Kmeria septentrionalis seed, and Kmeria septentrionalis seed is disappeared with the liquor potassic permanganate immersion 2h that mass fraction is 0.1%
Poison；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 2mmol/L3In solution, 24h
Afterwards the seed after immersion is taken out, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, after sealing
Lamination 100 days in 4 DEG C of constant incubators are placed in, lamination process opened the ventilative 40min of sack per 7 days, and kept the skin wet；It is described from
Equipped with sodium peroxide as oxygenating agent, oxygenating agent volume accounts for the 1.5% of seed and wet perlite cumulative volume for the bottom of envelope；
S3, vernalization：The surface of the seed after lamination is sprayed into mixed liquor, then the surface of the seed is carried out using uviol lamp
The irradiation of 25min, reuse infrared lamp carries out the irradiation of 15min to the surface of the seed, persistently stirs seed twice in irradiation process；
Again the seed after process is seeded in germination box, with wet perlite as matrix, is added with matrix and wet pearl
Rock volume ratio is 15:1.2 dodder extract, is then put into germination box in seed germination box, and seed germination box is set to：
12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately, open seed germination box in germination every 48h
A dodder extract is sprayed in box, terminates vernalization after 28 days；It is described mixing liquid and preparation method thereof be：Distill to 100 weight portions
Ozone gas being continually fed in water Ozone Water being obtained, ozone Measuring Ozone Concentration in Water is 5mg/L, is subsequently adding the carbonic acid of 3 weight portions
Sodium, 2 weight portion sodium acid carbonates, the talcum powder for adding 2 weight portions after dissolving stir, that is, mixed liquor is obtained；The Tu silks
Son extracts liquor manufacture method：The fresh seed of Chinese dodder with root is cleaned, it is drained, be cut into 1cm into segment after weigh 50g,
Then fully mix with 500mL absolute ethyl alcohols, 100mL distilled water, use quality fraction is that pH is adjusted to by 0.5% pyrolkigneous liquid
6.5, under 70KHz frequencies, ultrasound 20min, then stands 12h, the ultrasound 30min under 40KHz frequencies, then stands 36h,
Then with 4 layers of filtered through gauze 2 times, then gained filtrate will be filtered move into centrifuge tube, 3500r/min centrifugation 10min will be taken after centrifugation
Clear liquid, is evaporated off absolute ethyl alcohol in 80 DEG C of backspins, then plus 500mL distilled water dilutings, obtain dodder extract；
Wherein, in S2 and S3, wet perlitic absolute water content is maintained at 300%；Wet perlite preparation method is：By second
3 weight portion of alcohol, 0.2 weight portion of propane diols, 0.03 weight portion of hydroquinones, 0.3 weight portion of alanine, 0.3 weight portion of glutamic acid,
0.3 weight portion of malic acid, 0.3 weight portion of butanedioic acid are dissolved in the distilled water of 500 weight portions, are then passed through ozone gas system thereto
Soak is obtained, ozone concentration reaches 8mg/L in soak, perlite is soaked 5h in soak, that is, obtains wet perlite.
<Embodiment 9>
One kind breaks the seed dormant method of Kmeria septentrionalis, comprises the following steps：
S1, seed disinfection：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in September part, the shady and cool room of ventilation is stacked at
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin, obtains Kmeria septentrionalis seed, and Kmeria septentrionalis seed is disappeared with the liquor potassic permanganate immersion 2h that mass fraction is 0.1%
Poison；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 1.1mmol/L3In solution,
The seed after immersion is taken out after 18h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, sealing
After be placed in lamination 95 days in 4 DEG C of constant incubators, lamination process opened the ventilative 35min of sack per 7 days, and kept the skin wet；It is described
Equipped with sodium peroxide as oxygenating agent, oxygenating agent volume accounts for the 1.3% of seed and wet perlite cumulative volume for the bottom of valve bag；
S3, vernalization：The surface of the seed after lamination is sprayed into mixed liquor, then the surface of the seed is carried out using uviol lamp
The irradiation of 22min, reuse infrared lamp carries out the irradiation of 12min to the surface of the seed, persistently stirs seed twice in irradiation process；
Again the seed after process is seeded in germination box, with wet perlite as matrix, is added with matrix and wet perlite volume ratio
For 15:1.1 dodder extract, is then put into germination box in seed germination box, and seed germination box is set to：30 DEG C of illumination
Under the conditions of 12h, 12h under 20 DEG C of dark conditions, so alternately, every 42h open seed germination box germination box in spray
Dodder extract, terminated vernalization after 28 days；It is described mixing liquid and preparation method thereof be：Continue in 100 weight portion distilled water
Be passed through ozone gas be obtained Ozone Water, ozone Measuring Ozone Concentration in Water be 5mg/L, be subsequently adding 2.5 weight portions sodium carbonate, 1.5
Weight portion sodium acid carbonate, the talcum powder for adding 1.5 weight portions after dissolving stir, that is, mixed liquor is obtained；The seed of Chinese dodder
Extracting liquor manufacture method is：The fresh seed of Chinese dodder with root is cleaned, it is drained, be cut into 0.9cm into segment after weigh 50g,
Then fully mix with 500mL absolute ethyl alcohols, 100mL distilled water, use quality fraction is that pH is adjusted to by 0.5% pyrolkigneous liquid
5.5, under 65KHz frequencies, ultrasound 15min, then stands 11h, the ultrasound 25min under 35KHz frequencies, then stands 30h,
Then with 4 layers of filtered through gauze 2 times, then gained filtrate will be filtered move into centrifuge tube, 3500r/min centrifugation 10min will be taken after centrifugation
Clear liquid, is evaporated off absolute ethyl alcohol in 80 DEG C of backspins, then plus 500mL distilled water dilutings, obtain dodder extract；
Wherein, in S2 and S3, wet perlitic absolute water content is maintained at 250%；Wet perlite preparation method is：By second
2.5 weight portion of alcohol, 0.1 weight portion of propane diols, 0.02 weight portion of hydroquinones, 0.2 weight portion of alanine, 0.2 weight of glutamic acid
Part, 0.2 weight portion of malic acid, 0.2 weight portion of butanedioic acid are dissolved in the distilled water of 400 weight portions, are then passed through ozone gas thereto
Body is obtained soak, and in soak, ozone concentration reaches 8mg/L, perlite is soaked 4h in soak, that is, obtains wet pearl
Rock.
<Contrast test>
After by fresh Kmeria septentrionalis seed conventional method Stratificated treatment, sprouting test is then carried out, sprouting condition is sowing
In the soil under outdoor natural conditions, 28 days are sprouted, as a comparison case 1.By the sowing to embodiment 1～9 and comparative example 1
Kmeria septentrionalis seed sprouts initial time and germination rate is contrasted afterwards, draws following data：
1 each embodiment of table sprouts the impact of initial time and germination rate to after planting Kmeria septentrionalis seed
As can be seen from Table 1, Kmeria septentrionalis seed is sterilized, gibberellin and Stratificated treatment, brightness change vernalization, can break list
Property lily magnolia seed dormancy, effectively improve Kmeria septentrionalis percentage of seedgermination, the time required to effectively shortening Seed Germination；Add Tu
The sub- leaching liquor of silk, the wet perlitic absolute water content of holding, wet perlitic preparation, the use of oxygenating agent, to the kind after lamination
Sublist face sprays containing ozone, sodium carbonate, sodium acid carbonate and talcous mixed liquor and then seed is entered with uviol lamp, infrared lamp
The dormancy of Kmeria septentrionalis seed can be further broken in row irradiation, improve germination percentage.
Although embodiment of the present invention is disclosed as above, which is not restricted to listed by specification and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the embodiment with description.
Claims (10)
1. one kind breaks the seed dormant method of Kmeria septentrionalis, it is characterised in that comprise the following steps：
S1, seed disinfection：Kmeria septentrionalis seed is carried out disinfection；
S2, gibberellin and Stratificated treatment：Seed after sterilization is soaked in into the GA that concentration is 0.5～2mmol/L3In solution, 12～
The seed after immersion is taken out after 24h, then with wet perlite with 1:3 volume ratio mixing, loads in plastics valve bag, after sealing
It is placed in lamination 90～100 days in 4 DEG C of constant incubators；
S3, vernalization：Seed after lamination is seeded in germination box, with wet perlite as matrix, germination box is put into into kind then
In sub- germinating box, seed germination box is set to：12h under 30 DEG C of illumination conditions, 12h under 20 DEG C of dark conditions, so alternately,
Terminate vernalization after 28 days.
2. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that need before seed disinfection in S1
Through collecting seed and post-processing：The ripe Kmeria septentrionalis fruit of natural cracking is gathered in 9～October, is stacked at the shady and cool room of ventilation
It is interior, seed is picked after fruit fully ftractures, be placed in clear water after being dipped to kind of soft and soggyization, wash the red false kind of removal outer layer by rubbing with the hands
Skin.
3. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that seed disinfection method in S1
For：By the liquor potassic permanganate immersion 2h that seed mass fraction is 0.1%.
4. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that the bottom of valve bag in S2
Equipped with sodium peroxide as oxygenating agent, oxygenating agent volume accounts for the 1%～1.5% of seed and wet perlite cumulative volume.
5. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that earthing of seeds process in S2
In, the ventilative 30～40min of sack was opened per 7 days, and was kept the skin wet.
6. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that wet perlite in S2 and S3
Preparation method is：By 2～3 weight portion of ethanol, 0.1～0.2 weight portion of propane diols, 0.01～0.03 weight portion of hydroquinones, third
0.2～0.3 weight portion of propylhomoserin, 0.1～0.3 weight portion of glutamic acid, 0.1～0.3 weight portion of malic acid, 0.1～0.3 weight of butanedioic acid
Amount part is dissolved in the distilled water of 300～500 weight portions, is then passed through ozone gas thereto and soak, ozone in soak is obtained
Concentration reaches 8mg/L, perlite is soaked 3～5h in soak, that is, obtains wet perlite.
7. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that the seed in S3 after lamination
Need through following process before being placed in germination box：Mixed liquor is sprayed to the surface of the seed after lamination, then using uviol lamp to planting
Sublist face carries out the irradiation of 20～25min, and reuse infrared lamp carries out the irradiation of 10～15min to the surface of the seed, irradiates twice
During persistently stir seed；It is described mixing liquid and preparation method thereof be：Ozone gas system is continually fed into in 100 weight portion distilled water
Ozone Water is obtained, ozone Measuring Ozone Concentration in Water is 5mg/L, is subsequently adding sodium carbonate, 1～2 weight portion bicarbonate of 2～3 weight portions
Sodium, the talcum powder for adding 1～2 weight portion after dissolving stir, that is, mixed liquor is obtained.
8. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that also add in S3 mesostromas
It is 15 to have with wet perlite volume ratio:1～1.2 dodder extract；Dodder extract preparation method is：By fresh band root
The seed of Chinese dodder clean, it is drained, be cut into 0.8～1cm into segment after weigh 50g, then with 500mL absolute ethyl alcohols, 100mL
Distilled water is fully mixed, and use quality fraction is that pH is adjusted to 4.5～6.5 by 0.5% pyrolkigneous liquid, then at 60～70KHz frequencies
Lower ultrasonic 10～20min, then stands 10～12h, 20～30min of ultrasound under 30～40KHz frequencies, then stand 24～
36h, then with 4 layers of filtered through gauze 2 times, then will filter gained filtrate and move into centrifuge tube, and 3500r/min centrifugation 10min, after centrifugation
Take supernatant, be evaporated off absolute ethyl alcohol in 80 DEG C of backspins, then plus 500mL distilled water dilutings, obtain dodder extract.
9. break the seed dormant method of Kmeria septentrionalis as claimed in claim 8, it is characterised in that in S3 pregermination procedures every
36～48h opens seed germination box and sprays a dodder extract in germination box.
10. the seed dormant method of Kmeria septentrionalis is broken as claimed in claim 1, it is characterised in that wet pearl in S2 and S3
The absolute water content of rock is maintained at 200%～300%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108650929A (en) * | 2018-04-04 | 2018-10-16 | 付华玲 | A method of abolishing Kmeria septentrionalis seed dormancy |
| CN113179703A (en) * | 2021-05-28 | 2021-07-30 | 四川省中医药科学院 | Germination accelerating treatment method for stemona tuberosa seeds |
| CN113179700A (en) * | 2021-04-30 | 2021-07-30 | 烟台市森林资源监测保护服务中心(烟台市林业技术推广中心、烟台沿海防护林省级自然保护区管理中心) | Method for rapidly breaking dormancy of barberry seeds through pretreatment, temperature control and constant humidity |
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| CN103006131A (en) * | 2011-09-23 | 2013-04-03 | 张水珍 | Wet tissue |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108650929A (en) * | 2018-04-04 | 2018-10-16 | 付华玲 | A method of abolishing Kmeria septentrionalis seed dormancy |
| CN113179700A (en) * | 2021-04-30 | 2021-07-30 | 烟台市森林资源监测保护服务中心(烟台市林业技术推广中心、烟台沿海防护林省级自然保护区管理中心) | Method for rapidly breaking dormancy of barberry seeds through pretreatment, temperature control and constant humidity |
| CN113179703A (en) * | 2021-05-28 | 2021-07-30 | 四川省中医药科学院 | Germination accelerating treatment method for stemona tuberosa seeds |
| CN113179703B (en) * | 2021-05-28 | 2022-05-31 | 四川省中医药科学院 | Stemona tuberosa seed germination accelerating treatment method |
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Application publication date: 20170322 |