CN110959334A

CN110959334A - Germination culture medium for improving activity of tobacco seeds under low-temperature condition

Info

Publication number: CN110959334A
Application number: CN201911286741.XA
Authority: CN
Inventors: 郑昀晔; 马文广; 古吉; 牛永志; 乔雨
Original assignee: YUXI ZHONGYAN TOBACCO SEED CO Ltd
Current assignee: YUXI ZHONGYAN TOBACCO SEED CO Ltd
Priority date: 2019-12-14
Filing date: 2019-12-14
Publication date: 2020-04-07

Abstract

The invention discloses a germination culture medium for improving the activity of tobacco seeds under a low-temperature condition, wherein the germination culture medium is a starch solution, or a gibberellin solution, or a starch and gibberellin composite solution. The culture medium provided by the invention can effectively improve the activity of the tobacco seeds at a low temperature and promote the germination of the tobacco seeds.

Description

Germination culture medium for improving activity of tobacco seeds under low-temperature condition

Technical Field

The invention belongs to the field of plant seed vitality, and particularly relates to a germination culture medium for improving the vitality of tobacco seeds under a low-temperature condition.

Background

The temperature has a great influence on the seed germination, and the physiological and biochemical actions in the seeds need to be carried out under certain temperature conditions. Seeds are susceptible to loss of germination capacity at the highest or lowest temperature ranges, high temperatures denature seeds, and low temperatures inhibit seeds. Tobacco is an important economic crop in China, and high-quality tobacco seeds are the most basic guarantee measures for tobacco leaf production. The proper germination temperature of the tobacco seeds is 25-30 ℃, the tobacco seedlings are very sensitive to cold damage in the seedling raising process of the tobacco seeds, the seedling emergence time and seedling emergence quality of the tobacco seedlings can be seriously influenced by large day and night temperature difference, low temperature of a seedling raising pond, low temperature of air environment and the like. In addition, Yunnan is a big province of tobacco production, tobacco growers in early spring often adopt floating early sowing in the beginning of 1-2 months every year, the ground temperature of some places is still low, tobacco seeds germinate slowly under the condition of low temperature, the germination rate is low, and partial seeds are in a dormant state.

Therefore, in order to improve the activity of the tobacco seeds under the low-temperature condition, promote the germination of the tobacco seeds and effectively ensure the quality of tobacco seedlings, the invention needs to invent a germination culture medium for improving the activity of the tobacco seeds under the low-temperature condition.

Disclosure of Invention

The invention aims to provide a germination culture medium for improving the activity of tobacco seeds under a low-temperature condition.

The invention is realized in such a way that the germination culture medium is a starch solution, or a gibberellin solution, or a starch and gibberellin composite solution.

The invention has the following technical effects: under the condition of low temperature, the culture medium can obviously improve the germination rate and the germination index of tobacco seeds and shorten the germination time.

Detailed Description

The invention provides a germination culture medium for improving the activity of tobacco seeds under a low-temperature condition, wherein the germination culture medium is a starch solution or a gibberellin solution, or a starch and gibberellin composite solution.

The starch is soluble starch, and when the culture medium is a single starch solution, the mass concentration of the starch is 50 mg/L-500 mg/L.

The purity of the gibberellin is more than or equal to 98.0 percent, and when the culture medium is a single gibberellin solution, the mass concentration of the gibberellin solution is 2.50 mg/L-4.00 mg/L.

The mixture ratio of the starch and gibberellin composite solution is 100mg/L starch and 4.00mg/L gibberellin, or 500mg/L starch and 2.50mg/L gibberellin.

The invention has the beneficial effects that: the invention can obviously improve the activity of the tobacco seeds under the low-temperature condition, obviously improve the germination rate and the germination index of the tobacco seeds and shorten the germination time. The invention can help the tobacco seeds to effectively resist low-temperature freeze injury, improves the production efficiency and has strong application prospect.

Example 1

First, sample

And selecting the tobacco naked seeds of different varieties and preserved under different storage conditions for germination test. Sample specific information is shown in table 1.

Second, preparation of culture medium

The configuration combinations are shown in table 2.

Third, seed germination test

And (3) carrying out a seed germination test in a low-temperature (10-15 ℃) environment. The specific operation is as follows: and (3) filling 2 layers of wet filter paper in the culture dishes, uniformly placing 100 naked tobacco seeds to be detected in each culture dish, and covering. 3 times of germination are set for each sample to be detected, the sample to be detected germinates at the low temperature of 10-15 ℃ under the condition of 12h/12h of light culture/dark culture, the filter paper is kept wet during germination, and the germination number of the seeds is counted every day until the number reaches 21 d. And calculating the germination rate, the germination time and the germination index of the seeds.

Fourth, data processing and statistics

Germination rate (%) × (14 days germination number of seeds/number of seeds examined) × 100%;

germination time (d) ═ Σ (G)_t×D_t)/ΣG_t；

Germination index ═ sigma G_t/D_t；

In the formula, G_tTo obtain germination numbers at different times, D_tDays for germination.

Statistical data analysis was performed using Excel 2013, SPSS Statistics 24 software, significance was detected (P < 0.05).

Fifth, test results

The results are shown in Table 3: under the condition of low temperature, the culture medium provided by the invention obviously improves the germination rate and germination index of tobacco seeds and obviously shortens the germination time.

Claims

1. A germination culture medium for improving the activity of tobacco seeds under a low-temperature condition is characterized in that the germination culture medium is a starch solution, or a gibberellin solution, or a starch and gibberellin composite solution.

2. The germination medium for improving the viability of tobacco seeds under cryogenic conditions of claim 1, wherein the starch is soluble starch.

3. The germination medium for improving the activity of tobacco seeds under the low-temperature condition of claim 1, wherein the mass concentration of the starch solution is 50-500 mg/L.

4. The germination medium for improving the activity of tobacco seeds under the low-temperature condition of claim 1, wherein the gibberellin purity is greater than or equal to 98.0%.

5. The germination medium for improving the activity of tobacco seeds under the low-temperature condition as claimed in claim 1, wherein the mass concentration of the gibberellin solution is 2.50-4.00 mg/L.

6. The germination medium for improving the activity of tobacco seeds under the low-temperature condition of claim 1, wherein the ratio of the starch and gibberellin composite solution is 100mg/L starch and 4.00mg/L gibberellin.

7. The germination medium for improving the activity of tobacco seeds under the low-temperature condition of claim 1, wherein the ratio of the starch and gibberellin composite solution is 500mg/L starch and 2.50mg/L gibberellin.