CN101946692A - Tobacco compound pollen medium, preparation method and applications thereof - Google Patents
Tobacco compound pollen medium, preparation method and applications thereof Download PDFInfo
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- CN101946692A CN101946692A CN 201010252451 CN201010252451A CN101946692A CN 101946692 A CN101946692 A CN 101946692A CN 201010252451 CN201010252451 CN 201010252451 CN 201010252451 A CN201010252451 A CN 201010252451A CN 101946692 A CN101946692 A CN 101946692A
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Abstract
The invention discloses a tobacco compound pollen medium and a preparation method thereof. The tobacco compound pollen medium is prepared by respectively smashing and evenly mixing the following components in parts by weight: 25-30 parts of glucose, 25-30 parts of cane sugar, 40-49 parts of soluble starch, 0.5 part of sodium borate and 0.5 part of light calcium carbonate. The tobacco compound pollen medium can promote the germination of the pollen and the elongation of pollen tubes, improves the fertilization success rate, improves the tobacco seed yield at per unit, and lowers the seed production cost of the tobacco seeds. The method of the invention has simple operation, low cost, popularization and application values and good economic and social benefits.
Description
Technical field
The invention belongs to the tobacco artificial pollination technical field, be specifically related to the composite pollen medium that grows tobacco, also relate to the preparation method of this composite pollen medium simultaneously.
Background technology
In the tobacco male sterile line and the cross breeding seed course of work, artificial pollination is an important and requisite job.Because tobacco self botany characteristic, 1 flower has only 5 pieces of stamens, generates the pollen grain limited amount, and pollen collection difficulty often needs a large amount of male parents of plantation in the production, and drops into a large amount of manpowers collection pollen and satisfy maternal pollination needs, therefore, the pollen collection is the work that cost is high.At present, in tobacco male sterile line and cross breeding seed process, mainly use the laggard pedestrian worker's pollination of pure pollen of tobacco or single pollen medium and pure pollen proportioning.Pure pollen consumption is big, and the pollen effective rate of utilization is low, and production cost is too high; And the pollination effect is relatively poor after using single pollen medium and pure pollen proportioning.Be accompanied by the development process of China's tobacco agriculture, tobacco male sterile line and crossbreed are used rapidly and are promoted in China, and varieties of plant and area constantly increase.Therefore, in tobacco male sterile line and crossbreed artificial pollination process, how to promote the sprouting and the pollen tube growth of pollen, the final fertilization success rate that improves, under the prerequisite that guarantees seed quality, reduce the pollen consumption when improving the unit are seed production, effectively reduce breeding cost, just become problem demanding prompt solution in the tobacco production of hybrid seeds process.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, the composite pollen medium that grows tobacco is provided,, when improving pollen fertilization success rate, reduce the usage amount of pure pollen, reduce the seed production cost with instead of pure pollen or single pollen medium.
The present invention also aims to provide a kind of method for preparing described tobacco composite pollen medium.
Purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, percentage of the present invention is percetage by weight.
The one composite pollen medium that grows tobacco is made by the raw material of following weight parts: 25～30 parts of glucose, 25～30 parts of sucrose, 40～49 parts of soluble starches, 0.5 part of 0.5 part of borax and precipitated calcium carbonate; To mix after the described raw material pulverizing.
Prepare the method for described tobacco composite pollen medium, may further comprise the steps:
1, glucose, sucrose, soluble starch, borax are pulverized respectively, crossed 60 mesh sieves, precipitated calcium carbonate is crossed 600 mesh sieves, standby;
2, press 25～30 parts of glucose, 25～30 parts of sucrose, 40～49 parts of soluble starches, the ratio that 0.5 part of borax and precipitated calcium carbonate are 0.5 part mixes, and promptly obtains required tobacco composite pollen medium.
The application of described tobacco composite pollen medium is specially: described tobacco composite pollen medium and pure pollen are mixed the back pollination by 1: 1 weight ratio.
Have following advantage with respect to prior art the present invention: glucose, sucrose, soluble starch can provide energy and carbon source for the sprouting of pollen and the elongation of pollen tube; Borax promotes the elongation of pollen tube after being absorbed by column cap; Precipitated calcium carbonate can prevent composite pollen medium caking, can provide Ca under the column cap sour environment simultaneously
2+, promote the elongation of pollen tube.This tobacco composite pollen medium can improve the fertilization success rate, improves tobacco seed yield per unit area, reduces the tobacco seed breeding cost.The present invention is easy and simple to handle, and is with low cost, has a good application prospect.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but they are not limitation of the invention.
Embodiment 1
Powdered glucose, sucrose, soluble starch, borax are pulverized respectively, crossed 60 mesh sieves, precipitated calcium carbonate is crossed 600 mesh sieves, standby.The ratio of 25 parts of glucose, 25 parts of sucrose, 49 parts of soluble starches, 0.5 part of borax, 0.5 part of precipitated calcium carbonate mixes by weight, promptly obtains required composite pollen medium.
Embodiment 2
Repeat embodiment 1, following difference is arranged: in the ratio preparation composite pollen medium of 25 parts of glucose, 30 parts of sucrose, 45 parts of soluble starches, 0.5 part of borax, 0.5 part of precipitated calcium carbonate.
Embodiment 3
Repeat embodiment 1, following difference is arranged: in the ratio preparation composite pollen medium of 30 parts of glucose, 25 parts of sucrose, 45 parts of soluble starches, 0.5 part of borax, 0.5 part of precipitated calcium carbonate.
Embodiment 4
Repeat embodiment 1, following difference is arranged: in the ratio preparation composite pollen medium of 29 parts of glucose, 30 parts of sucrose, 40 parts of soluble starches, 0.5 part of borax, 0.5 part of precipitated calcium carbonate.
Application Example 1
In year May in September, 2009 to 2010, carry out field experiment in numerous base of China Tobacco Seed Yuxi Co., Ltd. Xishuangbanna winter.The tobacco composite pollen medium that embodiment 1 is made and cloud and mist 87, K326 and the pure pollen of cloud and mist 202 male parents mix in 1: 1 ratio, are applied in respectively on MS cloud and mist 87, MSK326 and the cloud and mist 202 maternal column caps with cotton swab, carry out artificial pollination.With pure pollen pollination is contrast.200 flowers of every strain pollination, 0.5 mu of each kind pollination, 3 repetitions.Gather by fruit in the brown ripe back of capsule, adopt the selection by winnowing mode to seed carry out selected after, dry to water content less than 5.0%, add up every mu of seed average yield.Carry out germination test under indoor 25 ℃, statistics germination vigor and germination rate.The result shows, use composite pollen medium and pure pollen mix the back pollination in 1: 1 ratio, average every mu of seed production of MS cloud and mist 87, MSK326 and cloud and mist 202 female parents reaches 12.41kg respectively, 11.99kg and 13.15kg, potentiality of seed all reaches more than 91.0%, and germination rate reaches more than 95.0%.(adopt pure pollen to pollinate with contrast ratio, every mu of seed average yield of MS cloud and mist 87 and MSK326 is respectively 8.98kg, 9.12kg and 9.85kg, germination vigor reaches more than 91.0%, germination rate is more than 95.0%), per mu yield has improved 38.2%, 31.5% and 33.5% respectively, and seed quality is unaffected.
Application Example 2
Repeated application embodiment 1, and following difference is arranged: the tobacco composite pollen medium that uses embodiment 2 to make.The result shows that MS cloud and mist 87, MSK326 and cloud and mist 202 seed average yield per mus reach 11.85kg respectively, and 11.47kg and 12.63kg, potentiality of seed all reach more than 91.0%, and germination rate reaches more than 95.0%.(adopt pure pollen to pollinate with contrast ratio, every mu of seed average yield of MS cloud and mist 87 and MSK326 is respectively 8.98kg, 9.12kg and 9.85kg, germination vigor reaches more than 91.0%, germination rate is more than 95.0%), per mu yield has improved 32.0%, 25.8% and 28.2% respectively, and seed quality is unaffected.
Application Example 3
Repeated application embodiment 1, and following difference is arranged: the tobacco composite pollen medium that uses embodiment 3 to make.The result shows that MS cloud and mist 87, MSK326 and cloud and mist 202 seed average yield per mus reach 12.07kg respectively, and 11.83kg and 12.90kg, potentiality of seed all reach more than 91.0%, and germination rate reaches more than 95.0%.(adopt pure pollen to pollinate with contrast ratio, every mu of seed average yield of MS cloud and mist 87 and MSK326 is respectively 8.98kg, 9.12kg and 9.85kg, germination vigor reaches more than 91.0%, germination rate is more than 95.0%), per mu yield has improved 34.4%, 29.7% and 31.0% respectively, and seed quality is unaffected.
Application Example 4
Repeated application embodiment 1, and following difference is arranged: the tobacco composite pollen medium that uses embodiment 4 to make.The result shows that MS cloud and mist 87, MSK326 and cloud and mist 202 seed average yield per mus reach 11.63kg respectively, and 11.70kg and 12.70kg, potentiality of seed all reach more than 91.0%, and germination rate reaches more than 95.0%.(adopt pure pollen to pollinate with contrast ratio, every mu of seed average yield of MS cloud and mist 87 and MSK326 is respectively 8.98kg, 9.12kg and 9.85kg, germination vigor reaches more than 91.0%, germination rate is more than 95.0%), per mu yield has improved 29.50%, 28.30% and 29.0% respectively, and seed quality is unaffected.
Claims (3)
1. the composite pollen medium that grows tobacco is made by the raw material of following weight parts: 25～30 parts of glucose, 25～30 parts of sucrose, 40～49 parts of soluble starches, 0.5 part of 0.5 part of borax and precipitated calcium carbonate; To mix after the described raw material pulverizing.
2. prepare the method for the described tobacco composite pollen of claim 1 medium, may further comprise the steps:
(1) glucose, sucrose, soluble starch, borax are pulverized respectively, crossed 60 mesh sieves, precipitated calcium carbonate is crossed 600 mesh sieves, standby;
(2) press 25～30 parts of glucose, 25～30 parts of sucrose, 40～49 parts of soluble starches, the ratio that 0.5 part of borax and precipitated calcium carbonate are 0.5 part mixes, and promptly obtains required tobacco composite pollen medium.
3. the application of the described tobacco composite pollen of claim 1 medium is specially: described tobacco composite pollen medium and pure pollen are mixed the back pollination by 1: 1 weight ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010252451 CN101946692B (en) | 2010-08-13 | 2010-08-13 | Tobacco compound pollen medium, preparation method and applications thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010252451 CN101946692B (en) | 2010-08-13 | 2010-08-13 | Tobacco compound pollen medium, preparation method and applications thereof |
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| CN101946692A true CN101946692A (en) | 2011-01-19 |
| CN101946692B CN101946692B (en) | 2012-08-15 |
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| CN 201010252451 Active CN101946692B (en) | 2010-08-13 | 2010-08-13 | Tobacco compound pollen medium, preparation method and applications thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103539507A (en) * | 2013-09-29 | 2014-01-29 | 四川省烟草公司凉山州公司 | Pollen starch culture medium and preparation method thereof |
| CN105123506A (en) * | 2015-09-08 | 2015-12-09 | 玉溪中烟种子有限责任公司 | Tobacco seed oil medium pollen and preparation method and purpose thereof. |
| CN110959334A (en) * | 2019-12-14 | 2020-04-07 | 玉溪中烟种子有限责任公司 | Germination culture medium for improving activity of tobacco seeds under low-temperature condition |
| CN115191452A (en) * | 2022-07-20 | 2022-10-18 | 云南大学 | Composite pollen medium, marigold composite medium pollen, and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1118649A (en) * | 1994-09-15 | 1996-03-20 | 湖南师范大学 | Application technology for storing pollen |
| CN101385437A (en) * | 2008-09-26 | 2009-03-18 | 玉溪中烟种子有限责任公司 | Solid medium pollen and preparation method and use thereof |
-
2010
- 2010-08-13 CN CN 201010252451 patent/CN101946692B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1118649A (en) * | 1994-09-15 | 1996-03-20 | 湖南师范大学 | Application technology for storing pollen |
| CN101385437A (en) * | 2008-09-26 | 2009-03-18 | 玉溪中烟种子有限责任公司 | Solid medium pollen and preparation method and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《云南农业大学学报》 20100731 邓盛斌等 不同花粉介质对烤烟授粉效果及种子质量的影响 475 1-3 第25卷, 第4期 2 * |
| 《林业科学研究》 20100430 律春燕等 黄牡丹花粉生活力测定方法的比较研究 272-276 1-3 第23卷, 第2期 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103539507A (en) * | 2013-09-29 | 2014-01-29 | 四川省烟草公司凉山州公司 | Pollen starch culture medium and preparation method thereof |
| CN103539507B (en) * | 2013-09-29 | 2015-04-01 | 四川省烟草公司凉山州公司 | Pollen starch culture medium and preparation method thereof |
| CN105123506A (en) * | 2015-09-08 | 2015-12-09 | 玉溪中烟种子有限责任公司 | Tobacco seed oil medium pollen and preparation method and purpose thereof. |
| CN110959334A (en) * | 2019-12-14 | 2020-04-07 | 玉溪中烟种子有限责任公司 | Germination culture medium for improving activity of tobacco seeds under low-temperature condition |
| CN115191452A (en) * | 2022-07-20 | 2022-10-18 | 云南大学 | Composite pollen medium, marigold composite medium pollen, and preparation method and application thereof |
| CN115191452B (en) * | 2022-07-20 | 2023-03-14 | 云南大学 | Composite pollen medium, marigold composite medium pollen and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101946692B (en) | 2012-08-15 |
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