CN101167421A - Cuttage propagation method for Pinus kesiya var. langbianensis - Google Patents
Cuttage propagation method for Pinus kesiya var. langbianensis Download PDFInfo
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- CN101167421A CN101167421A CNA2007100664322A CN200710066432A CN101167421A CN 101167421 A CN101167421 A CN 101167421A CN A2007100664322 A CNA2007100664322 A CN A2007100664322A CN 200710066432 A CN200710066432 A CN 200710066432A CN 101167421 A CN101167421 A CN 101167421A
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Abstract
The invention provides a cuttage propagation method for Pinus khasya which is easy and practical to operate. After the choice and process of cutting slips, the choice and process of cuttage media, grow seedlings and propagation, the survival rate of seedlings is above 80 percent, which is high. The seedlings can keep the good hereditary character of the mother plants to guarantee the coincidence between the seedlings and the mother plants. The time of growing seedlings is substantially shortened, the height of the seedlings can reach above 15cm and the ground diameter can reach above 0.15cm after one hundred days after cottage, besides the root system grows rapidly. The survival rate of the seedlings is above 95 percent in the current year and is above 90 percent in the next year. The invention provides technical assurance for sustainable development and utilization of the elite resource of Pinus khasya.
Description
Technical field
The present invention relates to a kind of pinus khasys cuttage breeding method, belong to the plant asexual multiplication technology field.
Background technology
Pinus khasys (Pinus kesiyavar.langbianensis) be Yunnan Province main its genetic improvement work is subjected to great attention always with material and resin tapping seeds, these seeds now have a large amount of seed orchards, seed production stand.Yet, the same with other seeds, the pinus khasys seed orchard also run into yield poorly, instability, and the offspring breaks up problems such as strong.Therefore, be necessary the problems referred to above of existing at the pinus khasys seed orchard, implement Developing Clonal Forestry and solve, and Developing Clonal Forestry at first Gonna breakthrough be exactly the pinus khasys vegetative propagation technique.
Summary of the invention
The present invention provides a kind of survival rate of seedling height for Developing Clonal Forestry just, and required time is short, can guarantee that new plant is with the corresponding to pinus khasys cuttage breeding method of elite stand fine quality.
The present invention finishes by following technical proposal: a kind of pinus khasys cuttage breeding method is characterized in that its process the following step:
The selection of A, plugged ear and processing: the annual skill bar that germinates in the cutting orchard that grows directly from seeds of selecting fine individual plant to build, long 7～the 9cm of plugged ear, clip end footpath 0.2～0.3cm, fringe bar otch straight snips, and behind the pine needle of the above 3～4cm scope of fringe bar base portion of erasing, with concentration is the indole-3-acetic acid solution soaking 1 hour of 100～150mg/L, to handle the plugged ear base portion;
B, cuttage: the plugged ear that the A step process is crossed is inserted in the matrix of following mass ratio:
Black earth in the pinus khasys sylvan life soil: Cortex Pini kesiyae=1: 1～2, control cutting medium total porosity is 50%～70%, and the control insertion depth is 3～4cm, after the insertion with the compacting and water permeable a little of matrix around the plugged ear, it is that keeping the cuttage seeding row spacing even is 8～12cm in 50% the plastic greenhouse that the plugged ear plant of cuttage is placed with two-layer shading screen and degree of shading;
C, breeding: after the cuttage in 2 months every day spray water 3～5 times, temperature is controlled at 20～28 ℃, humidity is controlled at 80%～90%, watered 1 time every 2～3 days after 2 months, behind the cuttage one and a half months, adjust degree of shading to 2 5%, to increase intensity of illumination, and carry out in the canopy ventilatively between 12 noon to 15 every day, relative moisture in the canopy being gradually reduced move into to 75%, two first quarter moon degree of shading is in front of the mountains hardening on carry out in 30% zone.
Black earth in the pinus khasys sylvan life soil in the described B step take from soil thickness be 30cm with interior topsoil, Cortex Pini kesiyae is the bark more than 3 years of becoming thoroughly decomposed, and granularity is crossed 4 mesh sieves.
The plastic seeding culturing bag of 10cm * 15cm is used in cuttage in the described B step, puts into wide 1.0～1.4m behind the seedling medium of packing in the bag, and the management in cuttage and later stage is convenient in the seedbed of long 8～12m.
Breeding in the described C step, cuttage executed in right amount every 20 days after 1 month concentration be 1 ‰ potassium dihydrogen phosphate and concentration be 2 ‰ carbendazim solution once.
The present invention is in the practical application in Yunnan Province, and the cuttage Best Times is annual March, and the cuttage and seedling culture time is 100 days, the afforestation of going up a hill July.
Pinus khasys cuttage breeding method provided by the invention, its practicality simple to operate, the seedling percent height, reach more than 80%, and seedling can effectively keep the good genetic character of elite stand, guaranteed the unanimity of new plant with the elite stand fine quality, seedling raise period shortens greatly, and after the cuttage 100 days, seedling height can reach more than the 15cm, more than the leading thread 0.15cm, and well developed root system, the survival rate of the afforestation of going up a hill then is more than 95%, and the storage rate in 1 year is more than 90%, grow fine, for the sustainable development and utilization of pinus khasys fine germplasm resources provides technical guarantee.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment 1
A, the selection of plugged ear and processing: between March, in the cutting orchard that grows directly from seeds of pinus khasys fine individual plant construction, choose annual germinating skill bar, the fringe bar lignification degree of this moment is not high, be beneficial to the quick formation of fringe bar callus, and then formation young root, it is full also to note selecting terminal bud when the fringe bar is chosen, and the training needle is blackish green, no damage by disease and insect, the germinating branch of robust growth, long 7～the 9cm of the plugged ear of cutting, base diameter 0.2～0.3cm, straight snips fringe bar otch is behind the pine needle of fringe bar base portion 3～4cm scope of erasing, be the indole-3-acetic acid solution soaking plugged ear base portion 1 hour of 106.6mg/L immediately with concentration, so that can do sth. in advance fringe bar rootage duration, and the root of hair number increases the raising seedling percent, shorten seedling raise period, guarantee the quality of nursery stock wood;
B, cuttage: the pinus khasys cottage propagation should be in conjunction with the actual conditions of mountain area afforestation, mostly be and set up the temporary nursery, mountain region, therefore the nursery must be prepared in advance, work mainly comprises whole ground and nursery sterilization, without the forest land of felling as the pinus khasys nursery of growing seedlings, at first should remove ground cover, the ground cover of felling generally removes with burning, the nursery of cleaning, by wide 1.5～1.8m, longly decide on landform, but should be no longer than the smooth seedbed that goes out the container bag placement of the standard of 10m, if nursery lot is opened up wasteland down in the Simao pine forest, then topsoil can be used as seedling medium and places bed surface standby, and sterilization mainly finger nursery bed disinfection in nursery is especially at the farmland of farming, grow seedlings in the frequent place of human activity such as nursery or garden, necessary strict sterilization, otherwise nursery stock will infect large tracts of land lethal diseases such as damping off, nursery sterilization: 1. can carry out in conjunction with the ground foreign material burn off in whole ground, the nursery scale removal process etc., adopt this method sterilization, if can burn under the less situation of foreign material on the ground, pile up the burn off sterilization but should outside the nursery, be transported into incinerated matter; 2. be 0.5% solution disinfection with carbendazim and potassium permanganate pulvis or the concentration of the two, concrete grammar: 8g/m is pressed in smooth good seedbed
2Carbendazim mixing 3g/m
2The potassium permanganate pulvis evenly let sterilization go, or be that 0.5% carbendazim solution evenly squirts seedbed 20cm topsoil with concentration, water the top layer, seedbed 1 time of same depth afterwards again with the liquor potassic permanganate of same concentrations;
The plastic seeding culturing bag of 10cm * 15cm is used in cuttage, pack in the bag and put into wide 1.2m behind the seedling medium, the seedbed of long 8m, use the Seedling bag at soil compaction edge around the seedbed, prevent that Seedling bag from collapsing, cutting medium is following mass ratio: the black earth in the pinus khasys sylvan life soil: the pinus khasys more than 3 years of becoming thoroughly decomposed crosses 4 mesh sieve barks=1: 2, soil thickness should be controlled at 30cm with interior topsoil when fetching earth, the total porosity of cutting medium is controlled at 55%, use this kind matrix, can guarantee the growth of seedling desired nutritional, increased soil porosity simultaneously, the moderate generation that can effectively avoid bad root phenomenon again of water retaining capacity can adapt to the bigger weather conditions of rainfall between the nursery stage in summer of Yunnan;
Before the cuttage, with concentration is that 0.5% carbendazim solution evenly squirts the grow seedling nutritional bag, liquor potassic permanganate with same concentrations waters Seedling bag 1 time more afterwards, during cuttage, the control insertion depth is 3～4cm, to transplant a cutting on every side matrix compacting and water permeable a little after inserting, the cuttage seeding row spacing is even to be 8cm, after cuttage is finished, on the seedbed, build the shed that height is 1.0m with plastic film immediately, real with the earth envelope around the shed, in order to avoid wind and prevent that insect from moving in the canopy, build two-layer degree of shading on the plastic greenhouse again and be 50% shading screen;
C, grow seedlings, breed: the temperature of shed of above-mentioned B process is controlled at 20～28 ℃, humidity is controlled at 80%～90%, should in time adjust temperature, humidity in the canopy when changing greatly, slotting machine is sprayed water 3～5 times every day in 2 months after the cuttage, temperature is controlled at below 28 ℃ in the seedbed, when being higher than 28 ℃, increase the water spray number of times; Watered water 1 time in per 2～3 days after 2 months, take one deck shading screen behind the cuttage one and a half months off, open plastic film one side between 12 noon to 15 every day and carry out in the canopy ventilatively, make that relative moisture gradually reduces to 75% in the canopy; Carry out the transition to behind two first quarter moons of cuttage and do not cover film, move into degree of shading and be the hardening of going up a hill in 30% the district of shading before the afforestation, cuttage is after 1 month, executes concentration every 20 days and be 1 ‰ potassium dihydrogen phosphate and concentration and be 2 ‰ carbendazim solution once; Also should in time weed with a hoe between nursery stage and carry out the extermination of disease and insect pest by existing mode, during to July, seedling height reaches more than the 15cm, more than the leading thread 0.15cm, and well developed root system, the afforestation of can going up a hill, survival rate and growth of young plantations then are all better.Go up a hill then afforestation survival rate more than 95%, the storage rate in 1 year grows fine more than 90%.
Embodiment 2
The selection of A, plugged ear and processing: between March, the annual skill bar that germinates in the cutting orchard that grows directly from seeds of selecting fine individual plant to build, long 7～the 9cm of plugged ear, clip end footpath 0.2～0.3cm, fringe bar otch straight snips, and behind the pine needle of the above 3～4cm scope of fringe bar base portion of erasing, at once be the indole-3-acetic acid solution soaking 1 hour of 145mg/L with concentration, to handle the plugged ear base portion, all the other operations and points for attention are identical with embodiment 1;
B, cuttage: the nursery before the cuttage is built and is sterilized with embodiment 1, and the plugged ear that the A step process is crossed is inserted in the matrix of following mass ratio:
Black earth in the pinus khasys sylvan life soil: Cortex Pini kesiyae=1: 1, it is that 30cm is with interior topsoil that black earth in the pinus khasys sylvan life soil is taken from soil thickness, Cortex Pini kesiyae is the bark more than 3 years of becoming thoroughly decomposed, and granularity is crossed 4 mesh sieves, control cutting medium total porosity is 65%, and the control insertion depth is 3～4cm, the plastic seeding culturing bag of 10cm * 15cm is used in cuttage, pack in the bag and put into wide 1.4m behind the seedling medium, the seedbed of long 10m, use the Seedling bag at soil compaction edge around the seedbed, prevent that Seedling bag from collapsing, with the compacting and water permeablely a little of matrix around the plugged ear, placing the plugged ear plant of cuttage with two-layer shading screen and degree of shading is in 50% the plastic greenhouse after the insertion, keeps that the cuttage seeding row spacing is even to be 12cm;
C, the breeding: after the cuttage in 2 months every day spray water 3～5 times, temperature is controlled at 20～28 ℃, humidity is controlled at 80%～90%, watered 1 time every 2～3 days after 2 months, behind the cuttage one and a half months, adjustment degree of shading is to 2 5%, to increase intensity of illumination, and carry out in the canopy ventilative between 12 noon to 15 every day, the interior relative moisture of canopy is gradually reduced to 75%, move into degree of shading behind two first quarter moons and be 30% zone carry out in front of the mountains hardening, cuttage executed in right amount every 20 days after 1 month concentration be 1 ‰ potassium dihydrogen phosphate and concentration be 2 ‰ carbendazim solution once.Also should in time weed with a hoe between nursery stage and carry out the extermination of disease and insect pest by existing mode, during to July, seedling height reaches more than the 15cm, more than the leading thread 0.15cm, and well developed root system, the afforestation of can going up a hill, survival rate and growth of young plantations then are all better.Go up a hill then afforestation survival rate more than 95%, the storage rate in 1 year grows fine more than 90%.
Claims (4)
1. pinus khasys cuttage breeding method is characterized in that its is through the following step:
The selection of A, plugged ear and processing: the annual skill bar that germinates in the cutting orchard that grows directly from seeds of selecting fine individual plant to build, long 7～the 9cm of plugged ear, clip end footpath 0.2～0.3cm, fringe bar otch straight snips, and behind the pine needle of the above 3～4cm scope of fringe bar base portion of erasing, with concentration is the indole-3-acetic acid solution soaking 1 hour of 100～150mg/L, to handle the plugged ear base portion;
B, cuttage: the cutting that the A step process is crossed is inserted in the matrix of following mass ratio:
Black earth in the pinus khasys sylvan life soil: Cortex Pini kesiyae=1: 1～2, control cutting medium total porosity is 50%～70%, and the control insertion depth is 3～4cm, matrix compacting and water permeable a little around will transplanting a cutting after the insertion, it is that keeping the cuttage seeding row spacing even is 8～12cm in 50% the plastic greenhouse that the cuttage plant is placed with two-layer shading screen and degree of shading;
C, grow seedlings, breed: after the cuttage in 2 months every day spray water 3～5 times, temperature is controlled at 20～28 ℃, humidity is controlled at 80%～90%, watered 1 time every 2～3 days after 2 months, behind the cuttage one and a half months, adjust degree of shading to 25%, to increase intensity of illumination, and carry out in the canopy ventilatively between 12 noon to 15 every day, relative moisture in the canopy being gradually reduced move into to 75%, two first quarter moon degree of shading is in front of the mountains hardening on carry out in 30% zone.
2. pinus khasys cuttage breeding method according to claim 1, it is characterized in that it is that 30cm is with interior topsoil that black earth in the pinus khasys sylvan life soil in the described B step is taken from soil thickness, Cortex Pini kesiyae is the bark more than 3 years of becoming thoroughly decomposed, and granularity is crossed 4 mesh sieves.
3. pinus khasys cuttage breeding method according to claim 1, the plastic seeding culturing bag that it is characterized in that the cuttage use 10cm * 15cm in the described B step, pack in the bag and put into wide 1.0～1.4m behind the seedling medium, the management in cuttage and later stage is convenient in the seedbed of long 8～12m.
4. pinus khasys cuttage breeding method according to claim 1 is characterized in that the breeding in the described C step, cuttage executed in right amount every 20 days after 1 month concentration be 1 ‰ potassium dihydrogen phosphate and concentration be 2 ‰ carbendazim solution once.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100664322A CN101167421A (en) | 2007-12-10 | 2007-12-10 | Cuttage propagation method for Pinus kesiya var. langbianensis |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100664322A CN101167421A (en) | 2007-12-10 | 2007-12-10 | Cuttage propagation method for Pinus kesiya var. langbianensis |
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| CN101167421A true CN101167421A (en) | 2008-04-30 |
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| CNA2007100664322A Pending CN101167421A (en) | 2007-12-10 | 2007-12-10 | Cuttage propagation method for Pinus kesiya var. langbianensis |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870579A (en) * | 2012-10-23 | 2013-01-16 | 泰安市泰山林业科学研究院 | Cutting propagation method of mono maple in seedling bags |
| CN104106360A (en) * | 2014-07-06 | 2014-10-22 | 普洱市林业科学研究所 | Method for shoot cuttage rejuvenation of superior adult trees of pinus khasys |
| CN104322226A (en) * | 2013-11-30 | 2015-02-04 | 钟诚 | Pine tree cutting seedling raising method |
| CN110178674A (en) * | 2019-05-28 | 2019-08-30 | 广西贺州市正丰现代农业股份有限公司 | A kind of cultural method of high yield Pachyrhizua angulatus |
| CN110463501A (en) * | 2019-09-19 | 2019-11-19 | 云南省林业科学院 | A kind of above Simao pine cuttage fringe bar rush of triennial sprouts method |
-
2007
- 2007-12-10 CN CNA2007100664322A patent/CN101167421A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870579A (en) * | 2012-10-23 | 2013-01-16 | 泰安市泰山林业科学研究院 | Cutting propagation method of mono maple in seedling bags |
| CN104322226A (en) * | 2013-11-30 | 2015-02-04 | 钟诚 | Pine tree cutting seedling raising method |
| CN104106360A (en) * | 2014-07-06 | 2014-10-22 | 普洱市林业科学研究所 | Method for shoot cuttage rejuvenation of superior adult trees of pinus khasys |
| CN104106360B (en) * | 2014-07-06 | 2016-04-27 | 普洱市林业科学研究所 | A kind of pinus khasys grows up the method for select tree epicormic branch cuttage childrenization |
| CN110178674A (en) * | 2019-05-28 | 2019-08-30 | 广西贺州市正丰现代农业股份有限公司 | A kind of cultural method of high yield Pachyrhizua angulatus |
| CN110463501A (en) * | 2019-09-19 | 2019-11-19 | 云南省林业科学院 | A kind of above Simao pine cuttage fringe bar rush of triennial sprouts method |
| CN110463501B (en) * | 2019-09-19 | 2022-03-29 | 云南省林业科学院 | Scion germination promoting method for cutting pinus khasys of 3 years old or older |
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Open date: 20080430 |