CN101076537B - 标记有多个荧光团的寡核苷酸 - Google Patents

标记有多个荧光团的寡核苷酸 Download PDF

Info

Publication number
CN101076537B
CN101076537B CN200480040647.1A CN200480040647A CN101076537B CN 101076537 B CN101076537 B CN 101076537B CN 200480040647 A CN200480040647 A CN 200480040647A CN 101076537 B CN101076537 B CN 101076537B
Authority
CN
China
Prior art keywords
oligonucleotide
labeled
probes
dye
dyes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200480040647.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN101076537A (zh
Inventor
毛飞
忻星
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AlleLogic Biosciences Corp
Original Assignee
AlleLogic Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AlleLogic Biosciences Corp filed Critical AlleLogic Biosciences Corp
Publication of CN101076537A publication Critical patent/CN101076537A/zh
Application granted granted Critical
Publication of CN101076537B publication Critical patent/CN101076537B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200480040647.1A 2003-11-19 2004-11-19 标记有多个荧光团的寡核苷酸 Expired - Fee Related CN101076537B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52326303P 2003-11-19 2003-11-19
US60/523,263 2003-11-19
PCT/US2004/038946 WO2005051967A2 (en) 2003-11-19 2004-11-19 Oligonucleotides labeled with a plurality of fluorophores

Publications (2)

Publication Number Publication Date
CN101076537A CN101076537A (zh) 2007-11-21
CN101076537B true CN101076537B (zh) 2012-07-04

Family

ID=34632765

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200480040647.1A Expired - Fee Related CN101076537B (zh) 2003-11-19 2004-11-19 标记有多个荧光团的寡核苷酸

Country Status (5)

Country Link
US (2) US7667024B2 (enExample)
EP (1) EP1689764B1 (enExample)
JP (2) JP2007534308A (enExample)
CN (1) CN101076537B (enExample)
WO (1) WO2005051967A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9410196B2 (en) 2008-09-03 2016-08-09 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006002167A2 (en) * 2004-06-17 2006-01-05 University Of Florida Research Foudation, Inc. Multi-acceptor molecular probes and applications thereof
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en) 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US8092784B2 (en) 2005-11-30 2012-01-10 Biotium, Inc. Enzyme substrate comprising a functional dye and associated technology and methods
AU2007233320B2 (en) * 2006-04-04 2013-12-12 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
CN101946180B (zh) 2007-12-19 2013-11-13 神谷来克斯公司 单分子检测用扫描分析器和使用方法
WO2011068569A1 (en) 2009-12-04 2011-06-09 Biotium, Inc. Heterocycle-substituted xanthene dyes
CN101709335B (zh) * 2009-12-10 2011-12-21 浙江大学 一种人乳头瘤病毒常见型四重荧光定量pcr分型检测方法
BR112012018394B8 (pt) * 2009-12-21 2021-07-27 Seegene Inc método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo
US20120258447A1 (en) * 2009-12-24 2012-10-11 Seegene, Inc Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
WO2012109157A2 (en) 2011-02-07 2012-08-16 The Governing Council Of The University Of Toronto Bioprobes and methods of use thereof
CN103502474B (zh) * 2011-05-06 2016-03-16 凯杰有限公司 包含经由连接基连结的标记的寡核苷酸
US9056885B2 (en) 2011-11-21 2015-06-16 Promega Corporation Carboxy X rhodamine analogs
EP2809813B1 (en) 2012-02-03 2018-08-08 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
EP2943586B1 (en) * 2012-12-27 2017-09-20 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay
EP4570924A3 (en) * 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
CA2906728C (en) 2013-03-15 2022-07-12 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
EP3019559A4 (en) 2013-08-22 2017-04-05 Sony Corporation Water soluble fluorescent or colored dyes and methods for their use
AU2014339884B2 (en) * 2013-10-25 2020-03-05 Biomadison, Inc. Enzyme assay with duplicate fluorophores
CN106460057A (zh) * 2014-06-02 2017-02-22 贝斯4创新公司 核苷酸多态性检测方法
WO2016081585A1 (en) * 2014-11-20 2016-05-26 Ampliwise Inc. Compositions and methods for nucleic acid amplification
EP3262022A4 (en) 2015-02-26 2018-08-15 Sony Corporation Phenylethynylnaphthalene dyes and methods for their use
KR102676814B1 (ko) 2015-02-26 2024-06-21 소니그룹주식회사 접합 그룹을 포함하는 수용성 형광 또는 착색 염료
JP2019503174A (ja) 2016-01-04 2019-02-07 クァンタムディーエックス グループ リミテッドQuantumdx Group Limited 電荷量タグを含む合成ヌクレオチドのデザイン、合成、および使用
WO2017173348A1 (en) 2016-04-01 2017-10-05 Sony Corporation Ultra bright dimeric or polymeric dyes with rigid spacing groups
KR102773661B1 (ko) 2016-04-01 2025-02-27 소니그룹주식회사 초휘도 이량체성 또는 중합체성 염료
RU2021124058A (ru) 2016-04-06 2021-09-10 Сони Корпорейшн Ультраяркие димерные или полимерные красители со спейсерными линкерными группами
EP3455238A1 (en) 2016-05-10 2019-03-20 Sony Corporation Ultra bright polymeric dyes with peptide backbones
JP7068192B2 (ja) 2016-05-10 2022-05-16 ソニーグループ株式会社 ポリマー染料およびシクロデキストリンを含む組成物、ならびにその使用
CA3021419A1 (en) 2016-05-11 2017-11-16 Sony Corporation Ultra bright dimeric or polymeric dyes
JP7068191B2 (ja) 2016-06-06 2022-05-16 ソニーグループ株式会社 蛍光または有色レポーター基を含むイオン性ポリマー
CN109642252A (zh) 2016-06-17 2019-04-16 加州理工学院 核酸反应及相关方法和组合物
WO2018022925A1 (en) 2016-07-29 2018-02-01 Sony Corporation Ultra bright dimeric or polymeric dyes and methods for preparation of the same
EP3691689A1 (en) 2017-10-05 2020-08-12 Sony Corporation Programmable dendritic drugs
KR20200067132A (ko) 2017-10-05 2020-06-11 소니 주식회사 프로그램가능한 중합체성 약물
KR20200083605A (ko) 2017-11-16 2020-07-08 소니 주식회사 프로그램가능한 중합체성 약물
CN111565756A (zh) 2018-01-12 2020-08-21 索尼公司 包含生物活性化合物的具有刚性间隔基团的聚合物
WO2019140227A1 (en) 2018-01-12 2019-07-18 Sony Corporation Phosphoalkyl ribose polymers comprising biologically active compounds
KR102742386B1 (ko) 2018-03-19 2024-12-16 소니그룹주식회사 형광 신호 향상을 위한 2가 금속의 사용
CN118480073A (zh) 2018-03-21 2024-08-13 索尼公司 具有连接体基团的聚合串联染料
WO2019204357A1 (en) 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
CN112313242A (zh) 2018-06-27 2021-02-02 索尼公司 具有含脱氧核糖的连接体基团的聚合物染料
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
CA3127102A1 (en) * 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
CN114040919A (zh) * 2019-07-05 2022-02-11 南京金斯瑞生物科技有限公司 一种荧光标记核酸及其合成方法
EP3861074A2 (en) 2019-09-26 2021-08-11 Sony Group Corporation Polymeric tandem dyes with linker groups
KR20220075399A (ko) * 2019-09-30 2022-06-08 소니그룹주식회사 뉴클레오티드 프로브
EP4256078A1 (en) 2020-12-07 2023-10-11 Sony Group Corporation Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
AU2022426779A1 (en) * 2021-12-29 2024-07-18 Co-Diagnostics, Inc. Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores
US20230366038A1 (en) 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)
US12347527B2 (en) * 2022-08-23 2025-07-01 Leica Microsystems Cms Gmbh Data storage device and method for storing data using an oligonucleotide nanostructure backbone and labels with dyes
WO2025207979A1 (en) * 2024-03-27 2025-10-02 3D Medicines Biomedical Technologies, Inc. Energy-transfer probes for multiplexed nucleic acid amplification assays

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US6150097A (en) * 1996-04-12 2000-11-21 The Public Health Research Institute Of The City Of New York, Inc. Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes
US6573047B1 (en) * 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
DE69610304T2 (de) * 1995-05-05 2001-04-05 Perkin-Elmer Corp., Foster City Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
JP2002515738A (ja) * 1996-01-23 2002-05-28 アフィメトリックス,インコーポレイティド 核酸分析法
PT912766E (pt) * 1996-06-04 2009-07-16 Univ Utah Res Found Monitorização da hibridização durante a pcr
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5696157A (en) * 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US6037137A (en) * 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6130101A (en) * 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6133445A (en) * 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
DE69939896D1 (de) * 1998-03-05 2008-12-24 Johnson & Johnson Res Pty Ltd Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits
GB2341189B (en) 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
US6472153B1 (en) * 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
GB2359809B (en) * 1999-12-24 2004-01-14 Sumitomo Chemical Co Phenylacetylene compound liquid crystal composition, polymer optically anisotropic product, and liquid crystal or optical element
JP5213297B2 (ja) * 2000-08-11 2013-06-19 ユニバーシティ オブ ユタ リサーチ ファウンデーション 単一ラベルオリゴヌクレオチド・プローブ
AU2001294859A1 (en) * 2000-09-29 2002-04-08 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150097A (en) * 1996-04-12 2000-11-21 The Public Health Research Institute Of The City Of New York, Inc. Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US6573047B1 (en) * 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9410196B2 (en) 2008-09-03 2016-08-09 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US9938573B2 (en) 2008-09-03 2018-04-10 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags

Also Published As

Publication number Publication date
US20050272053A1 (en) 2005-12-08
WO2005051967A2 (en) 2005-06-09
JP2007534308A (ja) 2007-11-29
WO2005051967A3 (en) 2007-08-16
EP1689764B1 (en) 2013-01-02
EP1689764A2 (en) 2006-08-16
CN101076537A (zh) 2007-11-21
US7667024B2 (en) 2010-02-23
EP1689764A4 (en) 2010-08-18
US20100240103A1 (en) 2010-09-23
JP2011254832A (ja) 2011-12-22
JP5307206B2 (ja) 2013-10-02

Similar Documents

Publication Publication Date Title
CN101076537B (zh) 标记有多个荧光团的寡核苷酸
JP4942484B2 (ja) 改良された感度および低バックグラウンドを備えるハイブリダイゼーションによるdna検出のための蛍光プローブ
JP4040087B2 (ja) Pcr増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬
US20230407376A1 (en) Two-part mediator probe
US6902900B2 (en) Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
JP4131749B2 (ja) 自己消光性蛍光プローブと方法
JP2002521069A (ja) 波長シフト性のプローブ及びプライマー
JP2004509613A (ja) 非同調的刺激pcr
US6803201B2 (en) Compositions and methods for polynucleotide sequence determination
CN101285094A (zh) 引物、成套引物、使用其的核酸扩增方法和变异检测方法
US8354523B2 (en) Oligonucleotide probes and uses thereof
JP3970816B2 (ja) バックグラウンドを下げる蛍光ハイブリダイゼーションプローブ
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
Bai et al. A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes
EP1585832B1 (en) Compositions and methods for polynucleotide detection
JP2004065262A (ja) 改善された蛍光共鳴エネルギー転移プローブ
ES2365737T3 (es) Métodos de amplificación.

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120704

Termination date: 20131119