CN101076537B - 标记有多个荧光团的寡核苷酸 - Google Patents
标记有多个荧光团的寡核苷酸 Download PDFInfo
- Publication number
- CN101076537B CN101076537B CN200480040647.1A CN200480040647A CN101076537B CN 101076537 B CN101076537 B CN 101076537B CN 200480040647 A CN200480040647 A CN 200480040647A CN 101076537 B CN101076537 B CN 101076537B
- Authority
- CN
- China
- Prior art keywords
- oligonucleotide
- labeled
- probes
- dye
- dyes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52326303P | 2003-11-19 | 2003-11-19 | |
| US60/523,263 | 2003-11-19 | ||
| PCT/US2004/038946 WO2005051967A2 (en) | 2003-11-19 | 2004-11-19 | Oligonucleotides labeled with a plurality of fluorophores |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101076537A CN101076537A (zh) | 2007-11-21 |
| CN101076537B true CN101076537B (zh) | 2012-07-04 |
Family
ID=34632765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200480040647.1A Expired - Fee Related CN101076537B (zh) | 2003-11-19 | 2004-11-19 | 标记有多个荧光团的寡核苷酸 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US7667024B2 (enExample) |
| EP (1) | EP1689764B1 (enExample) |
| JP (2) | JP2007534308A (enExample) |
| CN (1) | CN101076537B (enExample) |
| WO (1) | WO2005051967A2 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9410196B2 (en) | 2008-09-03 | 2016-08-09 | Quantumdx Group Limited | Methods and kits for nucleic acid sequencing |
| US10759824B2 (en) | 2008-09-03 | 2020-09-01 | Quantumdx Group Limited | Design, synthesis and use of synthetic nucleotides comprising charge mass tags |
Families Citing this family (55)
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| WO2006002167A2 (en) * | 2004-06-17 | 2006-01-05 | University Of Florida Research Foudation, Inc. | Multi-acceptor molecular probes and applications thereof |
| US8685711B2 (en) | 2004-09-28 | 2014-04-01 | Singulex, Inc. | Methods and compositions for highly sensitive detection of molecules |
| US9040305B2 (en) | 2004-09-28 | 2015-05-26 | Singulex, Inc. | Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry |
| US8092784B2 (en) | 2005-11-30 | 2012-01-10 | Biotium, Inc. | Enzyme substrate comprising a functional dye and associated technology and methods |
| AU2007233320B2 (en) * | 2006-04-04 | 2013-12-12 | Singulex, Inc. | Highly sensitive system and methods for analysis of troponin |
| CN101946180B (zh) | 2007-12-19 | 2013-11-13 | 神谷来克斯公司 | 单分子检测用扫描分析器和使用方法 |
| WO2011068569A1 (en) | 2009-12-04 | 2011-06-09 | Biotium, Inc. | Heterocycle-substituted xanthene dyes |
| CN101709335B (zh) * | 2009-12-10 | 2011-12-21 | 浙江大学 | 一种人乳头瘤病毒常见型四重荧光定量pcr分型检测方法 |
| BR112012018394B8 (pt) * | 2009-12-21 | 2021-07-27 | Seegene Inc | método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo |
| US20120258447A1 (en) * | 2009-12-24 | 2012-10-11 | Seegene, Inc | Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals |
| DE102010003781B4 (de) | 2010-04-08 | 2012-08-16 | Aj Innuscreen Gmbh | Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen |
| WO2012109157A2 (en) | 2011-02-07 | 2012-08-16 | The Governing Council Of The University Of Toronto | Bioprobes and methods of use thereof |
| CN103502474B (zh) * | 2011-05-06 | 2016-03-16 | 凯杰有限公司 | 包含经由连接基连结的标记的寡核苷酸 |
| US9056885B2 (en) | 2011-11-21 | 2015-06-16 | Promega Corporation | Carboxy X rhodamine analogs |
| EP2809813B1 (en) | 2012-02-03 | 2018-08-08 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| CN104662172B (zh) | 2012-08-03 | 2018-07-06 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
| EP2943586B1 (en) * | 2012-12-27 | 2017-09-20 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay |
| EP4570924A3 (en) * | 2013-01-24 | 2025-09-03 | California Institute of Technology | Chromophore-based characterization and detection methods |
| CA2906728C (en) | 2013-03-15 | 2022-07-12 | Nanobiosym, Inc. | Systems and methods for mobile device analysis of nucleic acids and proteins |
| EP3019559A4 (en) | 2013-08-22 | 2017-04-05 | Sony Corporation | Water soluble fluorescent or colored dyes and methods for their use |
| AU2014339884B2 (en) * | 2013-10-25 | 2020-03-05 | Biomadison, Inc. | Enzyme assay with duplicate fluorophores |
| CN106460057A (zh) * | 2014-06-02 | 2017-02-22 | 贝斯4创新公司 | 核苷酸多态性检测方法 |
| WO2016081585A1 (en) * | 2014-11-20 | 2016-05-26 | Ampliwise Inc. | Compositions and methods for nucleic acid amplification |
| EP3262022A4 (en) | 2015-02-26 | 2018-08-15 | Sony Corporation | Phenylethynylnaphthalene dyes and methods for their use |
| KR102676814B1 (ko) | 2015-02-26 | 2024-06-21 | 소니그룹주식회사 | 접합 그룹을 포함하는 수용성 형광 또는 착색 염료 |
| JP2019503174A (ja) | 2016-01-04 | 2019-02-07 | クァンタムディーエックス グループ リミテッドQuantumdx Group Limited | 電荷量タグを含む合成ヌクレオチドのデザイン、合成、および使用 |
| WO2017173348A1 (en) | 2016-04-01 | 2017-10-05 | Sony Corporation | Ultra bright dimeric or polymeric dyes with rigid spacing groups |
| KR102773661B1 (ko) | 2016-04-01 | 2025-02-27 | 소니그룹주식회사 | 초휘도 이량체성 또는 중합체성 염료 |
| RU2021124058A (ru) | 2016-04-06 | 2021-09-10 | Сони Корпорейшн | Ультраяркие димерные или полимерные красители со спейсерными линкерными группами |
| EP3455238A1 (en) | 2016-05-10 | 2019-03-20 | Sony Corporation | Ultra bright polymeric dyes with peptide backbones |
| JP7068192B2 (ja) | 2016-05-10 | 2022-05-16 | ソニーグループ株式会社 | ポリマー染料およびシクロデキストリンを含む組成物、ならびにその使用 |
| CA3021419A1 (en) | 2016-05-11 | 2017-11-16 | Sony Corporation | Ultra bright dimeric or polymeric dyes |
| JP7068191B2 (ja) | 2016-06-06 | 2022-05-16 | ソニーグループ株式会社 | 蛍光または有色レポーター基を含むイオン性ポリマー |
| CN109642252A (zh) | 2016-06-17 | 2019-04-16 | 加州理工学院 | 核酸反应及相关方法和组合物 |
| WO2018022925A1 (en) | 2016-07-29 | 2018-02-01 | Sony Corporation | Ultra bright dimeric or polymeric dyes and methods for preparation of the same |
| EP3691689A1 (en) | 2017-10-05 | 2020-08-12 | Sony Corporation | Programmable dendritic drugs |
| KR20200067132A (ko) | 2017-10-05 | 2020-06-11 | 소니 주식회사 | 프로그램가능한 중합체성 약물 |
| KR20200083605A (ko) | 2017-11-16 | 2020-07-08 | 소니 주식회사 | 프로그램가능한 중합체성 약물 |
| CN111565756A (zh) | 2018-01-12 | 2020-08-21 | 索尼公司 | 包含生物活性化合物的具有刚性间隔基团的聚合物 |
| WO2019140227A1 (en) | 2018-01-12 | 2019-07-18 | Sony Corporation | Phosphoalkyl ribose polymers comprising biologically active compounds |
| KR102742386B1 (ko) | 2018-03-19 | 2024-12-16 | 소니그룹주식회사 | 형광 신호 향상을 위한 2가 금속의 사용 |
| CN118480073A (zh) | 2018-03-21 | 2024-08-13 | 索尼公司 | 具有连接体基团的聚合串联染料 |
| WO2019204357A1 (en) | 2018-04-17 | 2019-10-24 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| CN112313242A (zh) | 2018-06-27 | 2021-02-02 | 索尼公司 | 具有含脱氧核糖的连接体基团的聚合物染料 |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| AU2019300169A1 (en) | 2018-07-13 | 2021-01-28 | Quantum-Si Incorporated | Biconjugatable labels and methods of use |
| CA3127102A1 (en) * | 2019-01-23 | 2020-07-30 | Quantum-Si Incorporated | High intensity labeled reactant compositions and methods for sequencing |
| CN114040919A (zh) * | 2019-07-05 | 2022-02-11 | 南京金斯瑞生物科技有限公司 | 一种荧光标记核酸及其合成方法 |
| EP3861074A2 (en) | 2019-09-26 | 2021-08-11 | Sony Group Corporation | Polymeric tandem dyes with linker groups |
| KR20220075399A (ko) * | 2019-09-30 | 2022-06-08 | 소니그룹주식회사 | 뉴클레오티드 프로브 |
| EP4256078A1 (en) | 2020-12-07 | 2023-10-11 | Sony Group Corporation | Spacing linker group design for brightness enhancement in dimeric or polymeric dyes |
| AU2022426779A1 (en) * | 2021-12-29 | 2024-07-18 | Co-Diagnostics, Inc. | Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores |
| US20230366038A1 (en) | 2022-05-16 | 2023-11-16 | Bioo Scientific Corporation | Compositions and methods relating to loop mediated isothermal amplification (lamp) |
| US12347527B2 (en) * | 2022-08-23 | 2025-07-01 | Leica Microsystems Cms Gmbh | Data storage device and method for storing data using an oligonucleotide nanostructure backbone and labels with dyes |
| WO2025207979A1 (en) * | 2024-03-27 | 2025-10-02 | 3D Medicines Biomedical Technologies, Inc. | Energy-transfer probes for multiplexed nucleic acid amplification assays |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5800996A (en) * | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
| US6150097A (en) * | 1996-04-12 | 2000-11-21 | The Public Health Research Institute Of The City Of New York, Inc. | Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes |
| US6573047B1 (en) * | 1999-04-13 | 2003-06-03 | Dna Sciences, Inc. | Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation |
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| US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
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| JP2002515738A (ja) * | 1996-01-23 | 2002-05-28 | アフィメトリックス,インコーポレイティド | 核酸分析法 |
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-
2004
- 2004-11-19 WO PCT/US2004/038946 patent/WO2005051967A2/en not_active Ceased
- 2004-11-19 JP JP2006541541A patent/JP2007534308A/ja not_active Withdrawn
- 2004-11-19 CN CN200480040647.1A patent/CN101076537B/zh not_active Expired - Fee Related
- 2004-11-19 EP EP04811635A patent/EP1689764B1/en not_active Expired - Lifetime
- 2004-11-19 US US10/993,625 patent/US7667024B2/en not_active Expired - Fee Related
-
2009
- 2009-12-23 US US12/646,514 patent/US20100240103A1/en not_active Abandoned
-
2011
- 2011-08-15 JP JP2011177659A patent/JP5307206B2/ja not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6150097A (en) * | 1996-04-12 | 2000-11-21 | The Public Health Research Institute Of The City Of New York, Inc. | Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes |
| US5800996A (en) * | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
| US6573047B1 (en) * | 1999-04-13 | 2003-06-03 | Dna Sciences, Inc. | Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9410196B2 (en) | 2008-09-03 | 2016-08-09 | Quantumdx Group Limited | Methods and kits for nucleic acid sequencing |
| US9938573B2 (en) | 2008-09-03 | 2018-04-10 | Quantumdx Group Limited | Methods and kits for nucleic acid sequencing |
| US10759824B2 (en) | 2008-09-03 | 2020-09-01 | Quantumdx Group Limited | Design, synthesis and use of synthetic nucleotides comprising charge mass tags |
Also Published As
| Publication number | Publication date |
|---|---|
| US20050272053A1 (en) | 2005-12-08 |
| WO2005051967A2 (en) | 2005-06-09 |
| JP2007534308A (ja) | 2007-11-29 |
| WO2005051967A3 (en) | 2007-08-16 |
| EP1689764B1 (en) | 2013-01-02 |
| EP1689764A2 (en) | 2006-08-16 |
| CN101076537A (zh) | 2007-11-21 |
| US7667024B2 (en) | 2010-02-23 |
| EP1689764A4 (en) | 2010-08-18 |
| US20100240103A1 (en) | 2010-09-23 |
| JP2011254832A (ja) | 2011-12-22 |
| JP5307206B2 (ja) | 2013-10-02 |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120704 Termination date: 20131119 |