CN101032207A - Method of inducing dragon trees planted by human beings to generate dragon's blood - Google Patents
Method of inducing dragon trees planted by human beings to generate dragon's blood Download PDFInfo
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- CN101032207A CN101032207A CN 200710100470 CN200710100470A CN101032207A CN 101032207 A CN101032207 A CN 101032207A CN 200710100470 CN200710100470 CN 200710100470 CN 200710100470 A CN200710100470 A CN 200710100470A CN 101032207 A CN101032207 A CN 101032207A
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Abstract
The present invention discloses method of utilizing exogenic chemical matter to induce dragon tree to generate dragon's blood. The method includes drilling holes in dragon tree trunk over three years old and dragon tree branch over two years old and injecting inducing matter, cutting longitudinal slots in the trunk and branch and filling the slots with absorbent cotton soaked with the inducing matter, wrapping the hole and the slot with transparent plastic film, cutting the branch or trunk becoming red completely with generated dragon's blood, drying and crushing, and extracting dragon's blood with methanol. The said method has obvious inducing effect and greatly shortened dragon's blood producing period, and is suitable for large scale dragon's blood production.
Description
Technical field
The present invention relates to a kind of exogenous chemical substances and induce dragon tree to produce the method for dragon's blood, relate in particular to a kind of exogenous chemical substances that utilizes and induce the artificial cultivation dragon tree to produce the method for dragon's blood.
Background technology
Dragon's blood is a kind of famous and precious and commonly used traditional Chinese medicine, has hemostasis, invigorates blood circulation, the effect of anti-inflammatory, analgesic therapy and myogenic.Modern pharmacology studies show that: dragon's blood has the microcirculation of the body of improvement, adjusts body metabolism, improves effects such as body's immunity, thereby is widely used clinically.Dragon's blood is mainly derived from two class plants: a class is that liliaceous plant swordleaf dragon tree platymiscium contains the resin (claiming also to claim that dragon's blood exhausts by domestic Dragon Blood) that extracts in the fat xylem and get, and in fact also mainly relies on from the Southeast Asia import; Another kind ofly be Palmae Daemonorops liane Sanguis Draxonis (DaemonoropsdracoBl.) and belong to stripping in his the plant species fruit together or the red resin of secretion (claiming the import dragon's blood).The history that China's traditional Chinese medical science is used dragon's blood has more than one thousand years, but for a long time, the dragon's blood raw material of China still depends on import.
Dracaena plant, the whole world have about 150 kinds, are distributed in the torrid zone and the subtropical zone in Asia and Africa.China originates in 5 kinds, mainly is distributed in ground such as Hainan, Xishuangbanna, Yunnan, Guangxi, Guangdong.The dragon tree poor growth, in vegetative kingdom its lifetime the longest, can reach 8,000 years, therefore be " the plant elderly person for whom a birthday celebration is being held " by what botanists praised.Dragon tree not only has important ornamental value, can do the indoor appreciation potted landscape, and that can do the public place again importantly intersperses ornamental plants, and it still refines rare Chinese medicine---the important raw and processed materials of " dragon's blood ".As the swordleaf dragon tree in the dracaena, Hainan dragon tree etc. is the raw material that refines dragon's blood, and its economic worth is high.Existing studies show that, domestic Dragon Blood are the immersion of the injured back of dragon tree trunk (as mechanical damage, insect cavity etc.) owing to Fusarium graminearum fungies such as (redness), and dragon tree plant itself produces a kind of defense response, and the sozin that forms promptly is called dragon's blood.Usually, the dragon's blood that obtains from dragon tree also claims that dragon's blood exhausts.
What dragon's blood exhausted obtains, and three kinds of methods are arranged at present:
1, gets its withered naturally tree bar or branch from wild dragon tree, refine dragon's blood with it again.The formation of dragon's blood is it in injured back owing to fungies such as having infected Fusarium graminearum forms in the wild dragon tree, and the process of its formation is slower, generally needs to go up century-old accumulation and just can cut down, and also can cause the exhaustion of resource because of continuous felling.At present, the dragon tree wild resource of peripheral country in Southeast Asia is exhausted, and the wild dragon tree resource of China is also exhausted, and last century, the eighties China just classified dragon tree as the protective plant rare in imminent danger of national III level protection.
2, utilize dragon tree in the process of tissue culture, in proliferated culture medium, make seedling produce the dragon's blood justacrine in medium, directly with the medium oven dry, use methanol extraction then, can obtain dragon's blood at cutting part by adding exogenous induction material matter.
3, fluting or boring on wild swordleaf dragon tree live body branch, the bacterial strain suspension that will separate, cultivate, extract from dragon tree injects wherein then, and with the airtight covering of plastic foil, handle after 6～12 months, the swordleaf dragon tree branch of handling is sawed, and drying, pulverizing back are used alcohol extract and are got.The inducing substance that this method is used belongs to microorganism fungus kind, and is applicable to wild swordleaf dragon tree, but present wild large tracts of land dragon tree resource in flakes is exhausted.
Summary of the invention
The purpose of this invention is to provide a kind of exogenous chemical substances that on artificial cultivation dragon tree or wild dragon tree, utilizes and induce the method for producing dragon's blood.
Dragon's blood production method proposed by the invention, be to utilize artificial cultivation or wild dragon tree, after tree body more than three ages done less than the spray in 2 years by its growth of amputation, external source chemical induction material is injected in the middle boring of limb more than biennial or biennial, around limb, carry out rip cutting 3-8 bar longitudinal furrow simultaneously, with being filled in the longitudinal furrow behind the absorbent cotton moisture absorption external source chemical induction material, embrace bundle with transparent plastic film or freshness protection package more then, seal at two.After producing red dragon's blood, the limb beginning of inducing this limb can be cut down dry, pulverizing back methanol extraction dragon's blood in 1 month in 5-10 month.Whole process comprises: the artificial cultivation process of dragon tree, the leaching process of inducing process and dragon's blood of dragon's blood.
The artificial cultivation process of described dragon tree is: the dragon tree seedling is planted in the field by seeding row spacing 1-2 rice * 2-3 rice specification, it carried out the truncation branch paramount 45-55 centimetre the time when dragon tree grows, and the 2-3 bar branch of on the branch of each truncation, selecting and remain; Again it is carried out truncation time branch when treating that branch grows to long 45-55 centimetre, the 2-3 bar second branch of on every branch, selecting and remain, and the like carry out, after third level branch grows, can no longer carry out truncation to it, allow its self-sow.Described dragon tree seedling is seed seedling, cuttage seeding or tissue cultivating seedling.
The process of inducing of described dragon's blood is: on the tame dragon tree branch below biennial is being cut out more than the life in 3 years, hole in limb centre more than biennial or biennial, 40 centimetres of hole depths or be deep to the branch position are injected inducing substance again, and are sealed with absorbent cotton in the hole; Again around this limb rip cutting 3-8 bar wide 1 centimetre, be deep to xylem, long 40 centimetres or to the longitudinal furrow at branch position, and with being filled in the longitudinal furrow behind the absorbent cotton moisture absorption inducing substance, and then embrace bundle with transparent plastic film or freshness protection package, seal at two.The limb beginning of inducing after 1 month produces red dragon's blood, and the whole xylem of handled branch all has been full of blood fat after 5-10 month, promptly all becomes redness, and can carry out its cutting-out the extraction of dragon's blood this moment.Described inducing substance is 6-BA.
The leaching process of described dragon's blood is: will induce the limb intercepting that produces blood fat, and after the drying and crushing, use methanol extraction, and promptly obtain dragon's blood.
The process of inducing of dragon's blood of the present invention also can be chosen on the wild dragon tree carries out.
Provided by the present inventionly on artificial cultivation dragon tree or wild dragon tree, utilize the exogenous chemical substances artificial induction to produce the method for dragon's blood, technology is simple, easy to operate, induce effect remarkable, can greatly shorten the cycle that dragon's blood is produced, being useful for the large-scale standardized commercialization produces, can solve the problem that to produce dragon's blood because of the dragon tree scarcity of resources in a large number well, can make regenerated resources obtain efficient and sustainable development, the equal highly significant of the economic benefit that is embodied, social benefit and ecological benefits.
Embodiment
The present invention is described in detail below in conjunction with example.
Involved in the present invention on artificial cultivation dragon tree or wild dragon tree, induce the method for producing dragon's blood by exogenous chemical substances, comprise the artificial cultivation process of dragon tree, the leaching process of inducing process and dragon's blood of dragon's blood.
1, the artificial cultivation process of dragon tree: choosing ground: select the water source convenient, soil layer is deep, the plot of fertile soil; Consolidation: earlier the soil is carried out two ploughs, two rakes, dig the cave by the specification of seeding row spacing 1-2 rice * 2-3 rice again, the specification in cave is 0.4 meter * dark 0.4 meter of mouthful wide 0.4 a meter * bottom width; Base manure: whenever spread manuer in holes into pig cow dung 10kg, superphosphate 0.25kg, composite fertilizer 0.1kg, urea 0.05kg; Nursery stock specification: 20-30 centimetre of high dragon tree seed seedling or cuttage seeding or tissue cultivating seedling; Plantation: the 3-8 month is best planting season, the water of drenching after planting; Cultivation management: the annual spraying fertilizer in plantation back 3-4 time, each 0.1-0.2kg composite fertilizer; Trunk management: grow and it is carried out the truncation branch paramount 45-55 centimetre the time, and the 2-3 bar branch of on the branch of each truncation, selecting and remain; Again it is carried out truncation time branch when treating that branch grows to long 45-55 centimetre, the 2-3 bar second branch of on every branch, selecting and remain equally again, and the like carry out, after third level branch grows, can no longer carry out truncation to it, allow its self-sow.
2, dragon's blood induce process: on the tame dragon tree branch below biennial is being cut out more than the life in 3 years, hole in limb centre more than biennial or biennial, aperture 1-2 centimetre, 40 centimetres of hole depths or to the branch position, in the hole, inject inducing substance again, stop up the aperture with absorbent cotton to full; Around limb rip cutting 3-8 bar wide 1 centimetre, be deep to xylem, long 40 centimetres or to the longitudinal furrow at branch position, with being filled in the longitudinal furrow behind the absorbent cotton moisture absorption inducing substance, or be filled in absorbent cotton and fully inject inducing substance in the longitudinal furrow again, at last with transparent plastic film or freshness protection package whole piece limb is embraced bundle again, seal at two.The limb beginning of inducing after about 1 month produces dragon's blood, and the 5-10 month, this limb xylem all became redness, can be used for extracting dragon's blood.Described inducing substance is 0.5-10.0mg/L 6-BA.
3, the leaching process of dragon's blood: will induce the limb intercepting that produces dragon's blood, methanol extraction is used in dry, pulverizing back, promptly obtains dragon's blood.Described process with the methanol extraction dragon's blood is with analyzing pure dissolve with methanol crushed material, and afterwards after filtration and abandon its residue, gained filtrate use petroleum ether extraction, leaves standstill layering afterwards; Treat that methanol layer reclaims solvent and add distilled water again to small size, make methanol concentration reach 28-35%, then filter again and obtain filtrate and filter residue; With filtrate chromatography on the Diaion resin, remove oligosaccharides with the distilled water wash-out again, use methanol-eluted fractions then again, use the SephadexLH-20 chromatography behind the recovery meoh eluate, be that eluant, eluent carries out wash-out with 45-55% methyl alcohol again, obtain several flow points; With gained flow point MCIgel post or SephadexLH-20 chromatographic purifying, the gained compound is the active ingredient of dragon's blood; With filter residue earlier with the mixed solution of benzinum-acetone be eluant, eluent with 200～300 order silica gel column chromatographies, obtain some flow point sections, again through the SephadexLH-20 column chromatography, distinguish purifying with the MCIgel post again, the gained compound is the active ingredient of dragon's blood.The ratio of described benzinum-acetone mixed solution PetroChina Company Limited.'s ether and acetone is 5: 3.
The process of inducing of dragon's blood of the present invention can be chosen on the wild dragon tree carries out.
Claims (4)
1, a kind of method of inducing dragon trees planted by human beings to generate dragon's blood is characterized in that comprising the artificial cultivation process of dragon tree, the leaching process of inducing process and dragon's blood of dragon's blood:
1), the artificial cultivation process of described dragon tree is the specification plantation dragon tree seedling by seeding row spacing 1-2 rice * 2-3 rice, carries out the truncation branch when treating the paramount 45-55 of seedling growth centimetre, and the 2-3 bar branch of selecting and remain on the branch of each truncation; Treat to carry out again when branch grows to long 45-55 centimetre truncation time branch, every branch 2-3 bar second branch of selecting and remain, and the like truncation no longer during to third level branch, allow its self-sow;
2), the process of inducing of described dragon's blood is on the tame dragon tree branch below biennial to be cut out more than the life in 3 years, hole in limb centre more than biennial or biennial, aperture 1-2 centimetre, 40 centimetres of hole depths or to the branch position, in the hole, inject inducing substance again, stop up the aperture with absorbent cotton to full; Around limb rip cutting 3-8 bar wide 1 centimetre, be deep to xylem, long 40 centimetres or to the longitudinal furrow at branch position, with being filled in the longitudinal furrow behind the absorbent cotton moisture absorption inducing substance, at last with transparent plastic film or freshness protection package whole piece limb is embraced bundle again, seal at two, treat that the limb xylem all becomes redness, is used to extract dragon's blood; Described inducing substance is 0.5-10.0mg/L 6-BA;
3), the leaching process of described dragon's blood is to induce the limb intercepting that produces dragon's blood, and is dry, pulverize the back and use methanol extraction, promptly obtains dragon's blood.
2, the method for inducing dragon trees planted by human beings to generate dragon's blood according to claim 1 is characterized in that: described dragon tree seedling is seed seedling, cuttage seeding or tissue cultivating seedling.
3, the method for inducing dragon trees planted by human beings to generate dragon's blood according to claim 1 is characterized in that: the process of inducing of described dragon's blood is to carry out on wild dragon tree.
4, the method for inducing dragon trees planted by human beings to generate dragon's blood according to claim 1 is characterized in that: the embankment formula of burying of inducing the process mesosulcus at dragon's blood is absorbent cotton to be filled in fully inject inducing substance in the longitudinal furrow again.
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| CNB2007101004705A CN100531559C (en) | 2007-04-12 | 2007-04-12 | Method of artificial cultivation for dragon trees to generate dragon's blood |
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| CNB2007101004705A CN100531559C (en) | 2007-04-12 | 2007-04-12 | Method of artificial cultivation for dragon trees to generate dragon's blood |
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| CN100531559C CN100531559C (en) | 2009-08-26 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928672A (en) * | 2010-07-02 | 2010-12-29 | 云南大学 | Fusarium prolifertum |
| CN101779578B (en) * | 2009-01-15 | 2011-08-31 | 中国医学科学院药用植物研究所 | Method for using fungus to elicit dragon tree to produce sangusis draconis |
| CN103283515A (en) * | 2013-05-29 | 2013-09-11 | 中国热带农业科学院热带生物技术研究所 | Method for artificially inducing dragon's blood |
| CN104418715A (en) * | 2013-09-03 | 2015-03-18 | 沈阳药科大学 | Polymer in daemonorops draco and preparation method and application of polymer |
| CN116267294A (en) * | 2022-12-06 | 2023-06-23 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1255151C (en) * | 2003-04-25 | 2006-05-10 | 云南云河药业有限公司 | Compound Longxue scorpion and preparation method thereof |
| CN1316876C (en) * | 2004-12-27 | 2007-05-23 | 中国热带农业科学院热带生物技术研究所 | Method for cultivating and producing dragon's blood using dragon dracaena tissue |
| CN100567479C (en) * | 2006-03-09 | 2009-12-09 | 中国科学院西双版纳热带植物园 | A kind of artificial culturing method of Sanguis Draxonis |
| CN1817105A (en) * | 2006-03-15 | 2006-08-16 | 中国科学院西双版纳热带植物园 | Production of Longxuejie by field simulated induction |
-
2007
- 2007-04-12 CN CNB2007101004705A patent/CN100531559C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101779578B (en) * | 2009-01-15 | 2011-08-31 | 中国医学科学院药用植物研究所 | Method for using fungus to elicit dragon tree to produce sangusis draconis |
| CN101928672A (en) * | 2010-07-02 | 2010-12-29 | 云南大学 | Fusarium prolifertum |
| CN103283515A (en) * | 2013-05-29 | 2013-09-11 | 中国热带农业科学院热带生物技术研究所 | Method for artificially inducing dragon's blood |
| CN104418715A (en) * | 2013-09-03 | 2015-03-18 | 沈阳药科大学 | Polymer in daemonorops draco and preparation method and application of polymer |
| CN104418715B (en) * | 2013-09-03 | 2016-08-24 | 沈阳药科大学 | Polymer in Sanguis Draxonis and its preparation method and application |
| CN116267294A (en) * | 2022-12-06 | 2023-06-23 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
| CN116267294B (en) * | 2022-12-06 | 2024-05-07 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
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| CN100531559C (en) | 2009-08-26 |
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