CN1817105A - Production of Longxuejie by field simulated induction - Google Patents
Production of Longxuejie by field simulated induction Download PDFInfo
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- CN1817105A CN1817105A CN 200610010747 CN200610010747A CN1817105A CN 1817105 A CN1817105 A CN 1817105A CN 200610010747 CN200610010747 CN 200610010747 CN 200610010747 A CN200610010747 A CN 200610010747A CN 1817105 A CN1817105 A CN 1817105A
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Abstract
An artificial inducing method for producing Dragon's blood includes such steps as slotting or drilling on the living branch of wild dragon tree, injecting the the Dragon's blood inducer in it, sealing it by plastic film for 6-12 months, sawing out said branch, drying, pulverizing, extracting in alcohol, and recovering the alcohol.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of field artificial induction and produce the method that dragon's blood exhausts.
Background technology
Dragon's blood is rare rare Chinese medicine, has promoting blood circulation and removing blood stasis, swelling and pain relieving, astringing to arrest bleeding, and regenerating tissue to heal wond, the effect of enriching blood are usually used in various mass formed by blood stasis, are called " with the panacea of blood " (seeing Compendium of Material Medica).Dragon's blood derives from the Western Regions at first, the Su Mendala that modern age is inferior southeast.The seventies, the Cai Xitao professor of Xishuangbanna Tropical Plant Garden, Chinese Academy of Sciences has found to produce the plant resources-swordleaf dragon tree (Dracaena cochinchinensis) of dragon's blood in south of Yunnan, and, develop domestic Dragon Blood by Phytochemistry and pharmaceutical research, be called dragon's blood and exhaust.Experimental results show that homemade dragon's blood exhaust with Compendium of Material Medica in the dragon's blood that comes by the Western Regions (dragon ' s blood) put down in writing the most approaching, can be used as the homemade alternative articles for use of Southeast Asia import dragon's blood fully.The existing dragon's blood of China exhausts family surplus the manufacturing enterprise ten, and containing tallow wood with swordleaf dragon tree is nearly kind more than 60 of the dragon's blood medicine produced of raw material, and annual value of production reaches more than 500,000,000 yuan.
Under nature, after swordleaf dragon tree damaged because of thunder and lightning, storm or phytophagy animal gnaw, the defensive compound owing to the intrusion of microorganism produces was dragon's blood and exhausts.This process is very slow, often decades-long and even upward century-old.Simultaneously, the swordleaf dragon tree resource is very rare, and is about to exhaustion, and the dragon's blood of natural formation exhausts not only and costs an arm and a leg, and its output also is difficult to satisfy the growing market demand.Therefore, raw material supply problem has become the bottleneck of restriction enterprise development, the development and use of restriction dragon's blood medicine.And to swordleaf dragon tree symbiosis fungi and with relation that dragon's blood exhausts formation a spot of preliminary discussion is only arranged in the prior art, though proposed to utilize the fungal induction dragon's blood to exhaust the hypothesis of generation, Shang Weijian has and utilizes activity fungal to induce in the field to produce the report that dragon's blood exhausts method.The inventor is through studying for a long period of time for this reason, developed a kind of Longxuejie inducing agent, this derivant and preparation method thereof is seen Chinese patent: 200610010733.9, and use this derivant that wild swordleaf dragon tree is implemented the artificial induction, solved above-mentioned technical problem well.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of field artificial induction to produce the method that dragon's blood exhausts, quicken the formation that dragon's blood exhausts, improve the cultivation benefit of swordleaf dragon tree, guarantee the raw material supply of dragon's blood drug manufacture by human intervention.
The invention provides a kind of field artificial induction and produce the method that dragon's blood exhausts, this method is made up of following steps:
1, select the branch of diameter 〉=3cm on the swordleaf dragon tree as inducing matrix, on selected branch, vertically sever 3～5 grooves with rubber tapping knife, groove depth 0.4cm～0.6cm, groove width 0.7cm～0.9cm, flute length 14cm～16cm, inter-alveolar distance 2～3cm, Longxuejie inducing agent is splashed in the groove, include the groove of derivant then with the airtight covering of plastic foil;
2, after 6～12 months, the swordleaf dragon tree branch of handling is sawed, dry, pulverize the back with 80%～90% alcohol extract, the extract that obtains behind the recovery ethanol is the field induced product.
A kind of field artificial induction provided by the invention produces the method that dragon's blood exhausts, and this method also can be made up of following steps:
1, the branch of selecting diameter 〉=3cm on the swordleaf dragon tree is as inducing matrix, electricity consumption to be drilled in the hole that gets out diameter 1～2cm on the selected branch, and the hole will penetrate branch, and each branch bores 2～5 holes, distance between borehole 3～5cm; In the Longxuejie inducing agent inlet hole, include the hole of derivant then with the airtight covering of plastic foil;
2, after 6～12 months, the swordleaf dragon tree branch of handling is sawed, dry, pulverize the back with 80%～90% alcohol extract, the extract that obtains behind the recovery ethanol is the field induced product.
Up to now, the development and utilization that dragon's blood exhausts all is the collection that relies on wild swordleaf dragon tree, not only cost an arm and a leg, and supply also can't guarantee.By enforcement of the present invention; can greatly shorten the formation time that dragon's blood exhausts; not only improve the planting benefit of swordleaf dragon tree, guaranteed the raw material supply of dragon's blood medicine large-scale production, and when helping hill farmer to shake off poverty and set out on the road to prosperity, also protected ecotope.Its method is easy, with low cost, has better market prospect.
Description of drawings
Fig. 1 induces disposition for what the field dragon's blood exhausted;
Fig. 2 induces the dragon's blood of handling gained for the field and exhausts sample.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail, but they are not limitation of the invention.
Embodiment 1
Selecting diameter on the swordleaf dragon tree is that the branch of 3cm is as inducing matrix, on selected branch, vertically sever 3 grooves with rubber tapping knife, groove depth 0.4cm, groove width 0.7cm, flute length 14cm, inter-alveolar distance 2cm, Longxuejie inducing agent is splashed in the groove, include the groove of derivant with the airtight covering of plastic foil then, and wrap up appropriate; After 6 months, the swordleaf dragon tree branch of handling is sawed, dry, pulverize the back with 80% alcohol extract, the extract that obtains behind the recovery ethanol is the field induced product.
Embodiment 2
Select diameter on the swordleaf dragon tree be the branch of 3cm as inducing matrix, electricity consumption to be drilled in the hole that gets out diameter 1cm on the selected branch, the hole radially penetrates branch, each branch bores 2 holes, distance between borehole 3cm; In the Longxuejie inducing agent inlet hole, include the hole of derivant with the airtight covering of plastic foil then, and wrap up appropriate; After 6 months, the swordleaf dragon tree branch of handling is sawed, dry, pulverize the back with 80% alcohol extract, the extract that obtains behind the recovery ethanol is the field induced product.
Contrast test:
Detection method: HPLC detects
Testing conditions: chromatographic column: the 20rbax-SB-C18 post (4.6mm * 250mm);
Flowing phase: 1% glacial acetic acid solution:
Acetonitrile=63: 37;
Detect wavelength: 260nm, 274nm;
Column temperature: about 30 ℃;
Sample size: 10 μ l;
Flow velocity: 1ml/min;
Test set A: be gained of the present invention field induced product, identify structure through mass spectrum and nuclear magnetic resonnance;
Control group B: for Nat'l Pharmaceutical ﹠ Biological Products Control Institute is used for the standard sample that dragon's blood exhausts quality inspection;
Testing result: the field is induced and is handled after 6 months in the products therefrom that the content of dragon's blood element is 1.2% among the test set A, and the content of dragon's blood element is 0.2% among the control group B, exhausts near the dragon's blood of formation naturally.
Testing result shows that this present invention quickens dragon's blood to exhaust formation and manually produce the reliable method that dragon's blood exhausts in the field.
Embodiment 3
Repeat embodiment 1, following difference is arranged: selecting diameter is the branch of 4cm, vertically severs 4 grooves on branch, groove depth 0.5cm, groove width 0.8cm, flute length 15cm, inter-alveolar distance 2.5cm.
Embodiment 4
Repeat embodiment 1, following difference is arranged: selecting diameter is the branch of 5cm, vertically severs 5 grooves on branch, groove depth 0.6cm, groove width 0.9cm, flute length 16cm, inter-alveolar distance 3cm.
Embodiment 5
Repeat embodiment 1 or 2, following difference is arranged: selecting diameter is the branch of 6cm.
Embodiment 6
Repeat embodiment 1 or 2, following difference is arranged: handled 8 months.
Embodiment 7
Repeat embodiment 1 or 2, following difference is arranged: handled 10 months.
Embodiment 8
Repeat embodiment 1 or 2, following difference is arranged: handled 12 months.
Embodiment 9
Repeat embodiment 2, following difference is arranged: electricity consumption is drilled in the hole that gets out diameter 1cm on the selected branch, and each branch bores 5 holes, distance between borehole 3cm.
Embodiment 10
Repeat embodiment 2, following difference is arranged: electricity consumption is drilled in the hole that gets out diameter 1.5cm on the selected branch, and each branch bores 4 holes, distance between borehole 4cm.
Embodiment 11
Repeat embodiment 2, following difference is arranged: electricity consumption is drilled in the hole that gets out diameter 2cm on the selected branch, and each branch bores 3 holes, distance between borehole 5cm.
Claims (2)
1, a kind of field artificial induction produces the method that dragon's blood exhausts, and this method is made up of following steps:
(1) select the branch of diameter 〉=3cm on the swordleaf dragon tree as inducing matrix, on selected branch, vertically sever 3～5 grooves with rubber tapping knife, groove depth 0.4cm～0.6cm, groove width 0.7cm～0.9cm, flute length 14cm～16cm, inter-alveolar distance 2～3cm, Longxuejie inducing agent is splashed in the groove, include the groove of derivant then with the airtight covering of plastic foil;
After (2) 6～12 months, the swordleaf dragon tree branch of handling is sawed, dry, pulverize the back with 80%～90% alcohol extract, the extract that obtains behind the recovery ethanol is the field induced product.
2, the described field artificial induction of claim 1 produces the method that dragon's blood exhausts, and this method is made up of following steps:
(1) branch of selecting diameter 〉=3cm on the swordleaf dragon tree is as inducing matrix, electricity consumption to be drilled in the hole that gets out diameter 1～2cm on the selected branch, and the hole will penetrate branch, and each branch bores 2～5 holes, distance between borehole 3～5cm; In the Longxuejie inducing agent inlet hole, include the hole of derivant then with the airtight covering of plastic foil;
After (2) 6～12 months, the swordleaf dragon tree branch of handling is sawed, dry, pulverize the back with 80%～90% alcohol extract, the extract that obtains behind the recovery ethanol is the field induced product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200610010747 CN1817105A (en) | 2006-03-15 | 2006-03-15 | Production of Longxuejie by field simulated induction |
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| CN 200610010747 CN1817105A (en) | 2006-03-15 | 2006-03-15 | Production of Longxuejie by field simulated induction |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100531559C (en) * | 2007-04-12 | 2009-08-26 | 中国热带农业科学院热带生物技术研究所 | Method of artificial cultivation for dragon trees to generate dragon's blood |
| CN109937843A (en) * | 2019-02-22 | 2019-06-28 | 霍山岳衡茧丝绸有限公司 | A kind of preparation method improving Dendrobidium huoshanness disease resistance ramulus mori matrix |
| CN116267294A (en) * | 2022-12-06 | 2023-06-23 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
-
2006
- 2006-03-15 CN CN 200610010747 patent/CN1817105A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100531559C (en) * | 2007-04-12 | 2009-08-26 | 中国热带农业科学院热带生物技术研究所 | Method of artificial cultivation for dragon trees to generate dragon's blood |
| CN109937843A (en) * | 2019-02-22 | 2019-06-28 | 霍山岳衡茧丝绸有限公司 | A kind of preparation method improving Dendrobidium huoshanness disease resistance ramulus mori matrix |
| CN116267294A (en) * | 2022-12-06 | 2023-06-23 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
| CN116267294B (en) * | 2022-12-06 | 2024-05-07 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
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