CN100510067C - 葡糖淀粉酶变体 - Google Patents

葡糖淀粉酶变体 Download PDF

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Publication number
CN100510067C
CN100510067C CNB008101329A CN00810132A CN100510067C CN 100510067 C CN100510067 C CN 100510067C CN B008101329 A CNB008101329 A CN B008101329A CN 00810132 A CN00810132 A CN 00810132A CN 100510067 C CN100510067 C CN 100510067C
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glucoamylase
variant
dna
sequence
seq
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Expired - Fee Related
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CNB008101329A
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Chinese (zh)
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CN1360630A (zh
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比贾恩·R·尼尔森
艾伦·斯文德森
亨里克·彼得森
杰斯珀·文德
汉尼·V·亨德里克森
托本·P·弗兰德森
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Novozymes AS
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Novozymes AS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/20Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB008101329A 1999-07-09 2000-07-07 葡糖淀粉酶变体 Expired - Fee Related CN100510067C (zh)

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DKPA199900999 1999-07-09
DKPA199900999 1999-07-09

Related Child Applications (1)

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CNA2009101412582A Division CN101550410A (zh) 1999-07-09 2000-07-07 葡糖淀粉酶变体

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CN1360630A CN1360630A (zh) 2002-07-24
CN100510067C true CN100510067C (zh) 2009-07-08

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CNB008101329A Expired - Fee Related CN100510067C (zh) 1999-07-09 2000-07-07 葡糖淀粉酶变体
CNA2009101412582A Pending CN101550410A (zh) 1999-07-09 2000-07-07 葡糖淀粉酶变体

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US (3) US7354753B2 (enExample)
EP (2) EP2009098A1 (enExample)
JP (1) JP2003504046A (enExample)
CN (2) CN100510067C (enExample)
AR (2) AR029759A1 (enExample)
AU (1) AU5805700A (enExample)
CA (1) CA2374009A1 (enExample)
WO (1) WO2001004273A2 (enExample)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6352851B1 (en) * 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
EP2009098A1 (en) * 1999-07-09 2008-12-31 Novozymes A/S Glucoamylase variant
CA2416967C (en) 2000-08-01 2014-02-18 Novozymes A/S Alpha-amylase mutants with altered properties
AU2002254876A1 (en) 2001-05-15 2002-11-25 Novozymes A/S Alpha-amylase variant with altered properties
AU2002333212A1 (en) * 2001-10-01 2003-04-14 Novozymes A/S Glucoamylase variants
JP4897186B2 (ja) * 2002-03-27 2012-03-14 花王株式会社 変異アルカリセルラーゼ
EP1576152B1 (en) 2002-12-17 2006-12-06 Novozymes A/S Thermostable alpha-amylases
EP1633878A1 (en) 2003-05-30 2006-03-15 Novozymes A/S Alcohol product processes
BRPI0412402A (pt) 2003-07-07 2006-09-05 Genencor Int polipeptìdeos de amilase termoestáveis, ácidos nucléicos codificando esses polipeptìdeos e seus usos
US8143048B2 (en) 2003-07-07 2012-03-27 Danisco A/S Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
CN1875097B (zh) 2003-10-28 2013-04-24 诺维信北美公司 杂交酶
DE102004026152A1 (de) * 2004-05-28 2005-12-15 Basf Ag Fermentative Herstellung von Feinchemikalien
DK2295557T3 (en) * 2004-07-07 2017-12-11 Genencor Int EXOAMYLASE VARIANTS
DK2365068T3 (en) 2004-12-22 2017-05-15 Novozymes As ENZYMER FOR PROCESSING STARCH
US8030050B2 (en) 2005-07-07 2011-10-04 Danisco A/S Modified amylases from Pseudomonas species
CN101268195A (zh) 2005-09-20 2008-09-17 诺维信北美公司 产生发酵产物的方法
WO2007076388A2 (en) 2005-12-22 2007-07-05 Novozymes North America, Inc. Processes for producing a fermentation product
CN100443589C (zh) * 2005-12-30 2008-12-17 湖南鸿鹰祥生物工程股份有限公司 高转化率高纯度糖化酶浓缩液的生产方法
CN101405397A (zh) 2006-03-22 2009-04-08 诺维信北美公司 发酵方法
RU2539776C2 (ru) 2006-06-19 2015-01-27 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Полипептид
JP5568307B2 (ja) 2006-10-10 2014-08-06 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン 修飾された性質を有するグルコアミラーゼ変異体
CN101668863B (zh) 2007-04-24 2014-12-03 诺维信北美公司 使经预处理的含木质纤维素的材料解毒
US9695549B2 (en) 2007-09-03 2017-07-04 Norozymes Als Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials
PL2479264T3 (pl) * 2007-10-09 2014-11-28 Danisco Us Inc Odmiany glukoamylazy o zmienionych właściwościach
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
EP3323889B1 (en) * 2007-11-20 2020-03-25 Danisco US Inc. Glucoamylase variants with altered properties
US8969034B2 (en) * 2008-04-18 2015-03-03 New England Biolabs, Inc. Endoglycosidases that cleave O-linked glycans
CA2726265A1 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
CN102083991A (zh) 2008-06-23 2011-06-01 诺维信公司 生产发酵产物的方法
US20110165617A1 (en) 2008-09-30 2011-07-07 Novozymes North America, Inc. Enzymatic Hydrolysis Of Pretreated Lignocellulose-Containing Material With Distillers Dried Grains
ES2526867T3 (es) 2008-11-20 2015-01-16 Novozymes Inc. Polipéptido que tiene actividad potenciadora amilolítica y polinucleótidos que codifican el mismo
WO2010078391A2 (en) 2008-12-30 2010-07-08 Novozymes North America, Inc. Improvement of enzymatic hydrolysis of pretreated lignocellulose-containing material with dissolved air flotation sludge
WO2010078392A2 (en) 2008-12-31 2010-07-08 Novozymes North America, Inc. Processes of producing fermentation products
EP2451961A1 (en) 2009-07-07 2012-05-16 Novozymes A/S Process for treating a substrate with an enzyme
CA2768315A1 (en) 2009-07-17 2011-01-20 Novozymes A/S A method of analyzing cellulose decay in cellulosic material hydrolysis
CN107299123A (zh) * 2009-08-19 2017-10-27 杜邦营养生物科学有限公司 葡糖淀粉酶的变体
WO2011039324A1 (en) 2009-09-30 2011-04-07 Novozymes A/S Steamed bread preparation methods and steamed bread improving compositions
WO2011076123A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Compositions comprising boosting polypeptide and starch degrading enzyme and uses thereof
CN102884197A (zh) 2010-01-29 2013-01-16 诺维信公司 具有酶预处理的沼气生产方法
WO2011100161A1 (en) 2010-02-09 2011-08-18 Novozymes North America, Inc. Addition of alpha - glucosidase and cobalt for producing fermentation products from starch
ES2636216T3 (es) 2010-03-30 2017-10-05 Novozymes North America, Inc. Procesos de producción de un producto de fermentación
ES2565060T3 (es) 2010-04-14 2016-03-31 Novozymes A/S Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
CA2802083C (en) 2010-06-11 2018-10-09 Novozymes A/S Enzymatic flour correction
US9308064B2 (en) 2010-07-26 2016-04-12 Johnson & Johnson Consumer Inc. Devices and methods for collecting and analyzing fluid samples from the oral cavity
CN103140586A (zh) 2010-08-02 2013-06-05 诺维信北美公司 产生发酵产物的方法
CA2817224A1 (en) 2010-11-19 2012-05-24 Novozymes North America, Inc. Processes of producing a fermentation product
US9816112B2 (en) 2010-12-22 2017-11-14 Novozymes A/S Processes for producing fermentation products
WO2012093041A1 (en) 2011-01-04 2012-07-12 Novozymes A/S Process for producing biogas from pectin and lignocellulose containing material
WO2012109119A2 (en) 2011-02-07 2012-08-16 Novozymes North America, Inc. Process of producing a fermentation product
US20140106427A1 (en) 2011-06-28 2014-04-17 Novozymes A/S Biogas From Enzyme-Treated Bagasse
ES2834102T3 (es) 2011-06-30 2021-06-16 Novozymes As Variantes de alfa-amilasa
WO2013006756A2 (en) 2011-07-06 2013-01-10 Novozymes A/S Alpha amylase variants and polynucleotides encoding same
BR112013032861A2 (pt) 2011-07-22 2017-01-24 Novozymes North America Inc métodos para aumentar a atividade da enzima celulolítica durante a hidrólise do material celulósico, para hidrolisar um material celulósico pré-tratado, para a produção de um produto da fermentação, e para a fermentação de um material celulósico pré-tratado
WO2013022948A1 (en) 2011-08-08 2013-02-14 The Coca-Cola Company Cell lines comprising endogenous taste receptors and their uses
ES2627987T3 (es) 2011-10-11 2017-08-01 Novozymes North America, Inc. Proceso de licuefacción para producir productos de fermentación
MX348696B (es) 2011-12-02 2017-06-26 Novozymes As Procesos para la produccion de productos de fermentacion.
WO2013083801A2 (en) 2011-12-09 2013-06-13 Novozymes A/S Biogas from substrates comprising animal manure and enzymes
EP2794899A1 (en) 2011-12-21 2014-10-29 Novozymes, Inc. Methods for determining the degradation of a biomass material
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
CN104334738B (zh) 2012-03-30 2019-01-29 诺维信北美公司 生产发酵产物的方法
MX2015001969A (es) 2012-08-17 2015-05-15 Novozymes As Variantes de asparaginasa termoestables y polinucleotidos que las codifican.
EP2893009A4 (en) 2012-09-07 2016-03-02 Novozymes As GLYCOAMYLASE VARIANTS AND POLYNUCLEOTIDE CODING THEREWITH, AND THEIR USE
CN104870631B (zh) * 2012-12-11 2025-03-25 丹尼斯科美国公司 表达来自烟曲霉的葡糖淀粉酶的里氏木霉宿主细胞和其使用方法
HUE040545T2 (hu) 2013-07-17 2019-03-28 Novozymes As Pullulanáz kimérák és az azokat kódoló polinukleotidok
EP3712274A1 (en) 2013-09-11 2020-09-23 Novozymes A/S Processes for producing fermentation products
HUE056192T2 (hu) 2014-01-22 2022-01-28 Novozymes As Pullulanázvariánsok és azokat kódoló polinukleotidok
EP3712240B1 (en) 2014-02-07 2023-09-27 Novozymes A/S Compositions for producing glucose syrups
WO2015143144A1 (en) 2014-03-19 2015-09-24 Novozymes A/S Method for enhancing activity of an x143 polypeptide
US11072786B2 (en) 2014-04-10 2021-07-27 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2016005452A1 (en) 2014-07-08 2016-01-14 Caravan Ingredients Inc. Sugar-producing and texture-improving bakery methods and products formed therefrom
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US20170321237A1 (en) 2014-12-01 2017-11-09 Novozymes A/S Improved Production of Glucose Syrups
CN108350442A (zh) 2015-06-18 2018-07-31 诺维信公司 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
US10597645B2 (en) 2015-12-22 2020-03-24 Novozymes A/S Process of extracting oil from thin stillage
US10067091B2 (en) 2016-07-29 2018-09-04 Saudi Arabian Oil Company Integrated sediment and water analysis device and method
WO2018075430A1 (en) 2016-10-17 2018-04-26 Novozymes A/S Methods of reducing foam during ethanol fermentation
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
BR112019021223A2 (pt) * 2017-04-11 2020-04-28 Novozymes A/S variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira.
AU2018262815B2 (en) * 2017-05-05 2024-06-13 New Matterhorn, Llc Glucose isomerase
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
EP3645555B1 (en) 2017-06-28 2021-12-08 Novozymes A/S Polypeptides having trehalase activity and polynucleotides encoding same
CN111094562A (zh) 2017-08-08 2020-05-01 诺维信公司 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
BR112020004476A2 (pt) 2017-09-15 2020-09-08 Novozymes A/S processo para produção de um produto de fermentação, e, uso de uma combinação de enzimas
MX2020003981A (es) 2017-10-23 2020-08-03 Novozymes As Procesos para reducir acido lactico en un sistema de fermentacion de biocombustible.
CA3089135A1 (en) 2018-01-29 2019-08-01 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
BR112020016677A2 (pt) 2018-02-15 2020-12-15 Novozymes A/S Levedura melhorada para a produção de etanol
CN112166197A (zh) 2018-05-31 2021-01-01 诺维信公司 用于增强酵母生长和生产力的方法
WO2020014407A1 (en) 2018-07-11 2020-01-16 Novozymes A/S Processes for producing fermentation products
EP3827088A1 (en) 2018-07-25 2021-06-02 Novozymes A/S Enzyme-expressing yeast for ethanol production
BR112021006692A2 (pt) 2018-10-08 2021-08-10 Novozymes A/S levedura expressando enzimas para produção de etanol
US11622751B2 (en) 2018-12-19 2023-04-11 Johnson & Johnson Consumer Inc. Devices and methods for collecting saliva samples from the oral cavity
EP3918060A1 (en) 2019-01-31 2021-12-08 Novozymes A/S Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed
US20220186266A1 (en) 2019-04-02 2022-06-16 Novozymes A/S Process For Producing A Fermentation Product
US20220264911A1 (en) 2019-07-09 2022-08-25 Dupont Nutrition Biosciences Aps Feed composition
CA3143381A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
BR112022002203A2 (pt) 2019-08-05 2022-09-06 Novozymes As Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa
CA3143527A1 (en) 2019-08-06 2021-02-11 Novozymes A/S Fusion proteins for improved enzyme expression
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
US12275967B2 (en) 2019-09-16 2025-04-15 Novozymes A/S Processes for producing fermentation products and compositions used therein
AU2020402990A1 (en) 2019-12-10 2022-06-09 Novozymes A/S Microorganism for improved pentose fermentation
CA3158832A1 (en) 2019-12-16 2021-06-24 Chee-Leong Soong Processes for producing fermentation products
WO2021163030A2 (en) 2020-02-10 2021-08-19 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
BR112023008361A2 (pt) 2020-11-02 2023-12-12 Novozymes As Variantes de glucoamilase e polinucleotídeos codificando as mesmas
EP4352241A1 (en) 2021-06-07 2024-04-17 Novozymes A/S Engineered microorganism for improved ethanol fermentation
JP2024536931A (ja) 2021-09-27 2024-10-08 インターナショナル エヌ アンド エイチ デンマーク エーピーエス 飼料添加組成物及びそれを使用する方法
JP2024539622A (ja) * 2021-10-15 2024-10-29 ダニスコ・ユーエス・インク グルコアミラーゼバリアント及びその使用方法
US20250319165A1 (en) 2022-05-17 2025-10-16 International N&H Denmark Aps Feed additive comprising enzyme combinations
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
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CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1(ce1)多肽和编码其的多核苷酸
AU2024262752A1 (en) 2023-04-28 2025-10-16 International N&H Denmark Aps Ruminant feed additive compositions
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000381A1 (en) * 1990-06-29 1992-01-09 Novo Nordisk A/S Enzymatic hydrolysis of starch to glucose, using a genetically engineered enzyme
WO1998003639A1 (en) * 1996-07-24 1998-01-29 Iowa State University Research Foundation, Inc. PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4272326A (en) 1980-01-24 1981-06-09 Allied Chemical Corporation Enhanced distillation of cyclohexanol from phenol with additional cyclohexanone feed
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
JPS62272987A (ja) 1986-05-22 1987-11-27 Hitachi Plant Eng & Constr Co Ltd ブドウ糖の製造方法
DK463887D0 (da) 1987-09-07 1987-09-07 Novo Industri As Gaerleader
JPH01191693A (ja) 1988-01-25 1989-08-01 Tdk Corp デンプンの連続的糖化方法
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
JP2733330B2 (ja) 1989-08-31 1998-03-30 日本食品化工株式会社 高純度澱粉糖の製造方法
CA2038485A1 (en) 1990-03-23 1991-09-24 Donald K. Hadden Nanofiltration process for making dextrose
DE69107455T3 (de) 1990-05-09 2004-09-23 Novozymes A/S Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
US5231017A (en) 1991-05-17 1993-07-27 Solvay Enzymes, Inc. Process for producing ethanol
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE4422198C2 (de) 1994-06-24 1997-08-28 Audi Ag Verfahren zum Steuern der elektrischen Beheizung eines Katalysators
DK0894126T3 (da) 1996-03-27 2006-06-12 Novozymes As Alkalisk phosphatase-deficient trådformet svamp
DK1032654T3 (da) 1997-11-26 2009-05-11 Novozymes As Thermostabil glucoamylase
US6255084B1 (en) * 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
US6352851B1 (en) * 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
US6329186B1 (en) * 1998-12-07 2001-12-11 Novozymes A/S Glucoamylases with N-terminal extensions
JP2002531121A (ja) * 1998-12-07 2002-09-24 ノボザイムス アクティーゼルスカブ N末端伸長を有するグルコアミラーゼ
EP2009098A1 (en) * 1999-07-09 2008-12-31 Novozymes A/S Glucoamylase variant
EP1280817A2 (en) * 2000-04-28 2003-02-05 Novozymes A/S Production and use of protein variants having modified immunogenecity
CN1875097B (zh) * 2003-10-28 2013-04-24 诺维信北美公司 杂交酶
US7464662B2 (en) * 2004-01-28 2008-12-16 Tokyo Electron Limited Compact, distributed inductive element for large scale inductively-coupled plasma sources

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000381A1 (en) * 1990-06-29 1992-01-09 Novo Nordisk A/S Enzymatic hydrolysis of starch to glucose, using a genetically engineered enzyme
WO1998003639A1 (en) * 1996-07-24 1998-01-29 Iowa State University Research Foundation, Inc. PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Effect of introducing proline residues on the stability of aspergillus awamori.. Yuxing Li,et al.Protein Engineering,Vol.10 No.10. 1997 *
Effect of replacing helical glycine residues with alanines on reversible and irreversible stability and production of aspergillus awamori glucoamylase.. Hsiu-mei Chen,et al.Protein Engineering,Vol.9 No.6. 1996 *

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EP1200566A2 (en) 2002-05-02
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US7927857B2 (en) 2011-04-19
AR029759A1 (es) 2003-07-16
AR069653A2 (es) 2010-02-10
US20120208243A1 (en) 2012-08-16
US20080213843A1 (en) 2008-09-04
WO2001004273A3 (en) 2001-09-07
AU5805700A (en) 2001-01-30
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US8722369B2 (en) 2014-05-13
CA2374009A1 (en) 2001-01-18

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