HUE056192T2 - Pullulanázvariánsok és azokat kódoló polinukleotidok - Google Patents
Pullulanázvariánsok és azokat kódoló polinukleotidokInfo
- Publication number
- HUE056192T2 HUE056192T2 HUE18182098A HUE18182098A HUE056192T2 HU E056192 T2 HUE056192 T2 HU E056192T2 HU E18182098 A HUE18182098 A HU E18182098A HU E18182098 A HUE18182098 A HU E18182098A HU E056192 T2 HUE056192 T2 HU E056192T2
- Authority
- HU
- Hungary
- Prior art keywords
- polynucleotides encoding
- encoding same
- pullulanase variants
- pullulanase
- variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2457—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01041—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14152110 | 2014-01-22 | ||
EP14195691 | 2014-12-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
HUE056192T2 true HUE056192T2 (hu) | 2022-01-28 |
Family
ID=52462896
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HUE18182098A HUE056192T2 (hu) | 2014-01-22 | 2015-01-21 | Pullulanázvariánsok és azokat kódoló polinukleotidok |
Country Status (7)
Country | Link |
---|---|
US (3) | US10030237B2 (hu) |
EP (2) | EP3097192B1 (hu) |
CN (1) | CN105960457B (hu) |
CA (1) | CA2932239C (hu) |
DK (2) | DK3097192T3 (hu) |
HU (1) | HUE056192T2 (hu) |
WO (1) | WO2015110473A2 (hu) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK3097192T3 (en) | 2014-01-22 | 2018-11-19 | Novozymes As | PULLULANASE VARIATIONS AND POLYNUCLEOTIDES CODING THEM |
RU2019134734A (ru) | 2014-02-07 | 2019-11-18 | Новозимс А/С | Композиции для получения глюкозных сиропов |
ES2743515T3 (es) | 2014-10-23 | 2020-02-19 | Novozymes As | Variantes de glucoamilasa y polinucleótidos que codifican las mismas |
WO2016087327A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains |
WO2017014974A1 (en) * | 2015-07-21 | 2017-01-26 | Novozymes A/S | Polypeptides having pullulanase activity suitable for use in liquefaction |
CN108384770B (zh) | 2018-03-01 | 2019-11-22 | 江南大学 | 一种降低环糊精对普鲁兰酶抑制作用的方法 |
CN109321552B (zh) * | 2018-10-11 | 2021-01-22 | 山东隆科特酶制剂有限公司 | 一种新型普鲁兰酶及其基因、工程菌和制备方法 |
US20220154226A1 (en) * | 2019-03-18 | 2022-05-19 | Novozymes A/S | Polypeptides Having Pullulanase Activity Suitable For Use In Liquefaction |
CN110343687B (zh) * | 2019-07-18 | 2023-05-09 | 南京林业大学 | 一种具有高分泌能力的普鲁兰酶突变体及其应用 |
CN111235135B (zh) * | 2020-03-16 | 2021-11-02 | 江南大学 | 一种中性普鲁兰酶突变体及其应用 |
CN113667662A (zh) * | 2021-07-05 | 2021-11-19 | 南京纽田科技有限公司 | 高性能淀粉脱支酶嵌合体菌种构建及其生产方法和应用 |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
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JPS5534046A (en) | 1978-09-01 | 1980-03-10 | Cpc International Inc | Novel glucoamyrase having excellent heat resistance and production |
US4316956A (en) | 1980-02-06 | 1982-02-23 | Novo Industri A/S | Fermentation process |
US4335208A (en) | 1980-03-11 | 1982-06-15 | Novo Industri A/S | Saccharification of starch hydrolysates |
JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
NO840200L (no) | 1983-01-28 | 1984-07-30 | Cefus Corp | Glukoamylase cdna. |
DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
US4536477A (en) | 1983-08-17 | 1985-08-20 | Cpc International Inc. | Thermostable glucoamylase and method for its production |
DE3475209D1 (en) | 1983-09-11 | 1988-12-22 | Gist Brocades Nv | Novel enzyme product and its use in the saccharification of starch |
US4587215A (en) | 1984-06-25 | 1986-05-06 | Uop Inc. | Highly thermostable amyloglucosidase |
US4628031A (en) | 1984-09-18 | 1986-12-09 | Michigan Biotechnology Institute | Thermostable starch converting enzymes |
JPS62126989A (ja) | 1985-11-26 | 1987-06-09 | Godo Shiyusei Kk | コルテイシウム属担子菌の生産する酵素を用いた澱粉質の無蒸煮糖化法 |
DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
US5989870A (en) | 1986-04-30 | 1999-11-23 | Rohm Enzyme Finland Oy | Method for cloning active promoters |
ATE80657T1 (de) | 1986-07-09 | 1992-10-15 | Novo Nordisk As | Mischungen von alpha-amylase zur verfluessigung von staerke. |
DE3886221T3 (de) | 1987-09-04 | 2000-12-21 | Novo Nordisk As | VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN -i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN -i(ASPERGILLUS). |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
CA2058633C (en) | 1989-06-13 | 2000-03-21 | Virgil B. Lawlis, Jr. | A method for killing cells without lysis |
US5162210A (en) | 1990-06-29 | 1992-11-10 | Iowa State University Research Foundation | Process for enzymatic hydrolysis of starch to glucose |
IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
US5231017A (en) | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
DE69325981T2 (de) | 1992-12-28 | 2000-02-03 | Genencor Int | Pullulanase, Mikroorganismen die sie produzieren, Verfahren zur Herstellung und ihre Anwendung |
FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
ES2165420T3 (es) | 1994-06-03 | 2002-03-16 | Novozymes Biotech Inc | Lacasas myceliophthora purificadas y acidos nucleicos que las codifican. |
CN101659926A (zh) | 1994-06-30 | 2010-03-03 | 诺沃奇梅兹有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
ES2329528T3 (es) | 1995-02-03 | 2009-11-26 | Novozymes A/S | Metodo para diseñar mutantes alfa-amilasa con propiedades determinadas. |
AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
KR19980702782A (ko) | 1995-03-09 | 1998-08-05 | 혼 마가렛 에이. | 녹말 액화 방법 |
BR9708887B1 (pt) | 1996-04-30 | 2014-10-29 | Novozymes As | "variante de uma alfa-amilase, uso da mesma, construção de dna, vetor de expressão recombinante, célula de bactéria ou fungo, aditivo e composição detergente". |
KR100530598B1 (ko) | 1997-06-10 | 2005-11-23 | 다카라 바이오 가부시키가이샤 | 초내열성 프로테아제 발현계 |
ATE423192T1 (de) | 1997-10-13 | 2009-03-15 | Novozymes As | Mutanten der alpha-amylase |
DK1032654T3 (da) | 1997-11-26 | 2009-05-11 | Novozymes As | Thermostabil glucoamylase |
US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
NZ505820A (en) | 1998-02-27 | 2002-10-25 | Novozymes As | Enzyme variants based on the 3D structure of maltogenic alpha-amylase that have an altered pH optimum, thermostability, specific activity, cleavage pattern and ability to reduce the staling of bread |
US6265197B1 (en) * | 1998-07-02 | 2001-07-24 | Novozymes A/S Krogshoejvej | Starch debranching enzymes |
CN1309701A (zh) | 1998-07-02 | 2001-08-22 | 诺沃奇梅兹有限公司 | 淀粉脱支酶 |
MXPA01000352A (es) | 1998-07-15 | 2002-06-04 | Novozymes As | Varientes de glucoamilasa. |
DE69932345T2 (de) | 1998-10-26 | 2007-07-19 | Novozymes A/S | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen |
CN100482801C (zh) | 1999-03-22 | 2009-04-29 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
ES2496568T3 (es) | 1999-03-30 | 2014-09-19 | Novozymes A/S | Variantes de alfa-amilasa |
AU5805700A (en) | 1999-07-09 | 2001-01-30 | Novozymes A/S | Glucoamylase variant |
US6350599B1 (en) * | 2000-01-12 | 2002-02-26 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
EP1250423B1 (en) * | 2000-01-12 | 2008-09-03 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
EP1370648A2 (en) | 2000-08-01 | 2003-12-17 | Novozymes A/S | Alpha-amylase mutants with altered properties |
EP1421187B1 (en) | 2001-07-27 | 2007-10-10 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Systems for in vivo site-directed mutagenesis using oligonucleotides |
WO2003048353A1 (en) | 2001-12-07 | 2003-06-12 | Novozymes A/S | Polypeptides having protease activity and nucleic acids encoding same |
ES2319757T5 (es) | 2002-01-23 | 2018-05-22 | Dsm Ip Assets B.V. | Fermentación de azúcares de pentosa |
ES2278210T3 (es) | 2002-12-17 | 2007-08-01 | Novozymes A/S | Alfa-amilasas termoestables. |
WO2004085627A1 (en) | 2003-03-26 | 2004-10-07 | Forskarpatent I Syd Ab | New saccharomyces cerevisiae strains utilizing xylose |
WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
ATE549403T1 (de) | 2003-06-25 | 2012-03-15 | Novozymes As | Enzyme zur stärkeverarbeitung |
ES2340390T3 (es) | 2003-10-28 | 2010-06-02 | Novozymes North America, Inc. | Enzimas hibridas. |
EP2365068B1 (en) | 2004-12-22 | 2017-03-01 | Novozymes A/S | Enzymes for starch processing |
US8021863B2 (en) * | 2007-02-19 | 2011-09-20 | Novozymes A/S | Polypeptides with starch debranching activity |
PL2222830T3 (pl) | 2007-12-12 | 2015-12-31 | Novozymes As | Sposób zacierania |
CN101965401B (zh) | 2008-03-07 | 2014-06-04 | 帝斯曼知识产权资产管理有限公司 | 发酵戊糖的细胞 |
CN102224245B (zh) | 2008-09-30 | 2016-01-13 | 诺维信股份有限公司 | 在丝状真菌细胞中使用阳性和阴性选择性基因的方法 |
JP5821132B2 (ja) | 2008-12-24 | 2015-11-24 | ディーエスエム アイピー アセッツ ビー.ブイ. | キシロース異性化酵素遺伝子及びペントース糖の発酵におけるそれらの使用 |
RU2560424C2 (ru) | 2009-02-20 | 2015-08-20 | ДАНИСКО ЮЭс ИНК. | Способ получения состава ферментационного бульона |
CA2782154C (en) | 2009-11-30 | 2018-10-16 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
CA2782036C (en) | 2009-12-01 | 2019-01-15 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
DK2510093T3 (en) | 2009-12-11 | 2018-06-06 | Novozymes North America Inc | protease Variants |
MX338068B (es) | 2010-04-14 | 2016-04-01 | Novozymes As | Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos. |
ES2673940T3 (es) | 2010-12-22 | 2018-06-26 | Novozymes North America, Inc. | Proceso para producir productos de fermentación a partir de materiales que contienen almidón |
EA201490216A1 (ru) | 2011-07-06 | 2014-07-30 | Новозимс А/С | Варианты альфа-амилазы и кодирующие их полинуклеотиды |
IN2014CN02469A (hu) | 2011-09-06 | 2015-06-19 | Novozymes As | |
MX351762B (es) | 2011-10-11 | 2017-10-26 | Novozymes As | Variantes de glucoamilasas y polinucleotidos que las codifican. |
CN102876650B (zh) * | 2012-07-23 | 2014-06-18 | 江南大学 | 一种普鲁兰酶突变体及其制备方法 |
DK3022300T3 (en) * | 2013-07-17 | 2018-10-22 | Novozymes As | : Pullulan chimeras and polynucleotides encoding them |
BR112016003339B1 (pt) * | 2013-08-30 | 2023-02-14 | Novozymes A/S | Composição de enzima, e, processo de produção de um produto de fermentação a partir de material contendo amido |
DK3097192T3 (en) | 2014-01-22 | 2018-11-19 | Novozymes As | PULLULANASE VARIATIONS AND POLYNUCLEOTIDES CODING THEM |
-
2015
- 2015-01-21 DK DK15703235.0T patent/DK3097192T3/en active
- 2015-01-21 HU HUE18182098A patent/HUE056192T2/hu unknown
- 2015-01-21 CA CA2932239A patent/CA2932239C/en active Active
- 2015-01-21 US US15/112,096 patent/US10030237B2/en active Active
- 2015-01-21 WO PCT/EP2015/051145 patent/WO2015110473A2/en active Application Filing
- 2015-01-21 EP EP15703235.0A patent/EP3097192B1/en active Active
- 2015-01-21 CN CN201580004962.7A patent/CN105960457B/zh active Active
- 2015-01-21 DK DK18182098.6T patent/DK3415624T3/da active
- 2015-01-21 EP EP18182098.6A patent/EP3415624B1/en active Active
-
2018
- 2018-06-25 US US16/016,950 patent/US10640755B2/en active Active
-
2020
- 2020-04-01 US US16/837,166 patent/US11136568B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
US20170044511A1 (en) | 2017-02-16 |
US10030237B2 (en) | 2018-07-24 |
US20180298364A1 (en) | 2018-10-18 |
CA2932239A1 (en) | 2015-07-30 |
CA2932239C (en) | 2023-03-14 |
DK3097192T3 (en) | 2018-11-19 |
DK3415624T3 (da) | 2021-11-08 |
CN105960457A (zh) | 2016-09-21 |
WO2015110473A3 (en) | 2015-09-17 |
EP3415624B1 (en) | 2021-08-04 |
EP3415624A1 (en) | 2018-12-19 |
US10640755B2 (en) | 2020-05-05 |
EP3097192B1 (en) | 2018-07-25 |
US11136568B2 (en) | 2021-10-05 |
US20200248157A1 (en) | 2020-08-06 |
CN105960457B (zh) | 2021-09-03 |
EP3097192A2 (en) | 2016-11-30 |
WO2015110473A2 (en) | 2015-07-30 |
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