CH636904A5 - Verfahren zur bestimmung von h2o2. - Google Patents
Verfahren zur bestimmung von h2o2. Download PDFInfo
- Publication number
- CH636904A5 CH636904A5 CH913877A CH913877A CH636904A5 CH 636904 A5 CH636904 A5 CH 636904A5 CH 913877 A CH913877 A CH 913877A CH 913877 A CH913877 A CH 913877A CH 636904 A5 CH636904 A5 CH 636904A5
- Authority
- CH
- Switzerland
- Prior art keywords
- nadh
- oxidase
- enzyme
- determination
- solution
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 19
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 22
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 14
- 102000004316 Oxidoreductases Human genes 0.000 claims description 13
- 108090000854 Oxidoreductases Proteins 0.000 claims description 13
- 108010084238 NAD+ peroxidase Proteins 0.000 claims description 12
- 239000007983 Tris buffer Substances 0.000 claims description 12
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 claims description 12
- 108010092464 Urate Oxidase Proteins 0.000 claims description 7
- 238000005859 coupling reaction Methods 0.000 claims description 7
- 108010025188 Alcohol oxidase Proteins 0.000 claims description 6
- 102000004674 D-amino-acid oxidase Human genes 0.000 claims description 6
- 108010003989 D-amino-acid oxidase Proteins 0.000 claims description 6
- 108010015776 Glucose oxidase Proteins 0.000 claims description 6
- 239000004366 Glucose oxidase Substances 0.000 claims description 6
- 108010008292 L-Amino Acid Oxidase Proteins 0.000 claims description 6
- 102000007070 L-amino-acid oxidase Human genes 0.000 claims description 6
- 230000008033 biological extinction Effects 0.000 claims description 6
- 230000029087 digestion Effects 0.000 claims description 6
- 229940116332 glucose oxidase Drugs 0.000 claims description 6
- 235000019420 glucose oxidase Nutrition 0.000 claims description 6
- 235000011056 potassium acetate Nutrition 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 5
- 108010089254 Cholesterol oxidase Proteins 0.000 claims description 4
- 241000194032 Enterococcus faecalis Species 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- LJCNDNBULVLKSG-UHFFFAOYSA-N 2-aminoacetic acid;butane Chemical compound CCCC.CCCC.NCC(O)=O LJCNDNBULVLKSG-UHFFFAOYSA-N 0.000 claims description 2
- 108010093894 Xanthine oxidase Proteins 0.000 claims description 2
- 102100033220 Xanthine oxidase Human genes 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 description 30
- 108090000790 Enzymes Proteins 0.000 description 30
- 229940088598 enzyme Drugs 0.000 description 30
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 15
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000000872 buffer Substances 0.000 description 11
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 8
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 8
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 8
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 8
- 238000011084 recovery Methods 0.000 description 8
- 229940116269 uric acid Drugs 0.000 description 8
- 235000012000 cholesterol Nutrition 0.000 description 7
- 229940107161 cholesterol Drugs 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 6
- 238000007430 reference method Methods 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008351 acetate buffer Substances 0.000 description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 229940075420 xanthine Drugs 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 4
- 239000004395 L-leucine Substances 0.000 description 4
- 235000019454 L-leucine Nutrition 0.000 description 4
- 150000001298 alcohols Chemical class 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229960003136 leucine Drugs 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- QNAYBMKLOCPYGJ-UWTATZPHSA-N D-alanine Chemical compound C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 3
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 229920002873 Polyethylenimine Polymers 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 150000001840 cholesterol esters Chemical class 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 description 2
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 2
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108010007843 NADH oxidase Proteins 0.000 description 2
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 150000002334 glycols Chemical class 0.000 description 2
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 2
- 238000006395 oxidase reaction Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000007974 sodium acetate buffer Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- RYCLIXPGLDDLTM-UHFFFAOYSA-J tetrapotassium;phosphonato phosphate Chemical compound [K+].[K+].[K+].[K+].[O-]P([O-])(=O)OP([O-])([O-])=O RYCLIXPGLDDLTM-UHFFFAOYSA-J 0.000 description 2
- QGEACRIKUUTESN-UHFFFAOYSA-K tripotassium triacetate Chemical compound [K+].[K+].[K+].CC([O-])=O.CC([O-])=O.CC([O-])=O QGEACRIKUUTESN-UHFFFAOYSA-K 0.000 description 2
- PJKPEFPGGBAAJE-UHFFFAOYSA-N 1-hydroxy-3,7-dihydropurine-2,6-dione Chemical class O=C1N(O)C(=O)NC2=C1NC=N2 PJKPEFPGGBAAJE-UHFFFAOYSA-N 0.000 description 1
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
- BKAJNAXTPSGJCU-UHFFFAOYSA-N 4-methyl-2-oxopentanoic acid Chemical compound CC(C)CC(=O)C(O)=O BKAJNAXTPSGJCU-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 241000271533 Crotalus durissus terrificus Species 0.000 description 1
- 150000008574 D-amino acids Chemical class 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
- 102000003983 Flavoproteins Human genes 0.000 description 1
- 108010057573 Flavoproteins Proteins 0.000 description 1
- 108010008488 Glycylglycine Proteins 0.000 description 1
- 150000008575 L-amino acids Chemical class 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 108010055297 Sterol Esterase Proteins 0.000 description 1
- 102000000019 Sterol Esterase Human genes 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 102000020006 aldose 1-epimerase Human genes 0.000 description 1
- 108091022872 aldose 1-epimerase Proteins 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 229960000458 allantoin Drugs 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- MKKIWWXKPGIMGN-UHFFFAOYSA-N boric acid;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound OB(O)O.OC(=O)CC(O)(C(O)=O)CC(O)=O MKKIWWXKPGIMGN-UHFFFAOYSA-N 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940050410 gluconate Drugs 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229940043257 glycylglycine Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 229920000831 ionic polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- OSHVGUAPPIYWIS-UHFFFAOYSA-N piperazin-1-ium;acetate Chemical compound CC(O)=O.C1CNCCN1 OSHVGUAPPIYWIS-UHFFFAOYSA-N 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- -1 propionate ions Chemical class 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2633728A DE2633728C3 (de) | 1976-07-27 | 1976-07-27 | NADH-Peroxidase, ihre Gewinnung und Verwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH636904A5 true CH636904A5 (de) | 1983-06-30 |
Family
ID=5984055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH913877A CH636904A5 (de) | 1976-07-27 | 1977-07-22 | Verfahren zur bestimmung von h2o2. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4186052A (OSRAM) |
| JP (1) | JPS5814198B2 (OSRAM) |
| CH (1) | CH636904A5 (OSRAM) |
| DE (1) | DE2633728C3 (OSRAM) |
| FI (1) | FI56845C (OSRAM) |
| FR (1) | FR2359850A1 (OSRAM) |
| GB (1) | GB1528309A (OSRAM) |
| IT (1) | IT1081816B (OSRAM) |
| NL (1) | NL188045C (OSRAM) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2806371A1 (de) * | 1978-02-10 | 1979-08-23 | Guenther Kohlbecker | Verfahren zur enzymatischen bestimmung von oxalat mittels oxalat-oxidase |
| GB2015532B (en) * | 1978-02-27 | 1982-06-16 | Yamasa Shoyu Kk | -lysine -oxidase |
| EP0007176A3 (en) * | 1978-06-12 | 1980-02-20 | Cetus Corporation | Method for producing epoxides and glycols from alkenes |
| US4341868A (en) | 1978-08-18 | 1982-07-27 | Kyowa Hakko Kogyo Co., Ltd. | Method and test composition for the determination of the substrate for xanthine oxidase |
| WO1980000454A1 (fr) * | 1978-08-18 | 1980-03-20 | Kyowa Hakko Kogyo Kk | Methode de mesure d'un substrat de xanthine oxydase et composition pour sa determination |
| DE3477812D1 (en) * | 1983-01-12 | 1989-05-24 | Alcoholism & Drug Addiction | Rapid analysis of ethanol in body fluids |
| DE3313178A1 (de) * | 1983-04-12 | 1984-10-18 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur enzymatischen bestimmung von sulfit |
| JPS59205999A (ja) * | 1983-05-10 | 1984-11-21 | Wako Pure Chem Ind Ltd | 還元型補酵素の定量方法 |
| JPS6131097A (ja) * | 1984-07-23 | 1986-02-13 | Toyo Jozo Co Ltd | 新規な高感度酵素的測定法 |
| JPS62140999A (ja) * | 1985-12-16 | 1987-06-24 | 川鉄機材工業株式会社 | 重量物懸吊装置 |
| DE3725851A1 (de) * | 1986-08-12 | 1988-02-18 | Takara Shuzo Co | Nad(p)h-oxidase, verfahren zu ihrer isolierung und anwendung |
| US5385827A (en) * | 1989-09-20 | 1995-01-31 | Clark; John R. | Method of geochemical prospecting |
| MX389199B (es) * | 2014-10-31 | 2025-03-20 | Rohm & Haas | Proceso de esterificación oxidativa para elaboración de metacrilato de metilo. |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT986838B (it) * | 1972-05-12 | 1975-01-30 | Sclavo Inst Sieroterapeut | Complesso di reagenti per la deter minazione enzimatica del glucosio sistema glucosio ossidasi perossi dasi con metodo manuale e automati co con lettura a termine o in cinetica |
-
1976
- 1976-07-27 DE DE2633728A patent/DE2633728C3/de not_active Expired
-
1977
- 1977-06-07 IT IT24454/77A patent/IT1081816B/it active
- 1977-06-09 NL NLAANVRAGE7706337,A patent/NL188045C/xx not_active IP Right Cessation
- 1977-06-29 US US05/811,422 patent/US4186052A/en not_active Expired - Lifetime
- 1977-07-13 FI FI772186A patent/FI56845C/fi not_active IP Right Cessation
- 1977-07-22 CH CH913877A patent/CH636904A5/de not_active IP Right Cessation
- 1977-07-25 GB GB31143/77A patent/GB1528309A/en not_active Expired
- 1977-07-27 JP JP52090216A patent/JPS5814198B2/ja not_active Expired
- 1977-07-27 FR FR7723161A patent/FR2359850A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| IT1081816B (it) | 1985-05-21 |
| NL188045C (nl) | 1992-03-16 |
| US4186052A (en) | 1980-01-29 |
| NL7706337A (nl) | 1978-01-31 |
| FR2359850B1 (OSRAM) | 1980-04-18 |
| JPS5315492A (en) | 1978-02-13 |
| DE2633728B2 (de) | 1978-10-05 |
| DE2633728A1 (de) | 1978-02-02 |
| DE2633728C3 (de) | 1979-07-12 |
| FR2359850A1 (fr) | 1978-02-24 |
| NL188045B (nl) | 1991-10-16 |
| FI56845C (fi) | 1980-04-10 |
| JPS5814198B2 (ja) | 1983-03-17 |
| GB1528309A (en) | 1978-10-11 |
| FI56845B (fi) | 1979-12-31 |
| FI772186A7 (OSRAM) | 1978-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CH636904A5 (de) | Verfahren zur bestimmung von h2o2. | |
| DE2265121B2 (de) | Enzympräparat zur Bestimmung von Cholesterin und Verfahren zu seiner Herstellung | |
| DE3686597T2 (de) | Uricase und verfahren zu deren herstellung. | |
| DE2817087C3 (de) | Glycerinoxidase und Verfahren zu ihrer Herstellung | |
| EP0033540A2 (de) | Verfahren und Reagens zur Bestimmung von Glycerin | |
| DE3436005C2 (de) | Neue Bilirubinoxidase, Verfahren zu ihrer Herstellung und diese enthaltende Reagenszubereitung | |
| DE2649749C2 (OSRAM) | ||
| DE69704328T2 (de) | Verfahren zur quantitativen Bestimmung von 1,5-Anhydroglucitol, sowie Reagenz zur quantitativen Bestimmung | |
| DE69121456T2 (de) | Stabilisiertes Harnsäurereagens | |
| EP0117550B1 (de) | Pyruvatoxidase | |
| EP0120440A2 (de) | NAD(P)-unabhängige Glycerindehydrogenase, Verfahren zu deren Herstellung sowie deren Verwendung zur Bestimmung von Glycerin und Triglyceriden | |
| DE3600563C2 (OSRAM) | ||
| DE2509156A1 (de) | Verfahren zur bestimmung der cholesteringesamtmenge in einer serumprobe und einzelreagens zur durchfuehrung dieses verfahrens | |
| EP0112571B1 (de) | Verfahren und Reagenz zur Bestimmung von N-Carbamoylsarcosin und hierfür geeignetes neues Enzym | |
| DE2737288C2 (de) | Verfahren zur Bestimmung des freien und/oder in Form von Triglyceriden vorliegenden gebundenen Glycerins in wäßrigen Flüssigkeiten sowie Bestimmungsreagenz für die Durchführung des Verfahrens und Bestimmungsreagenz für die Bestimmung von Adenosintriphosphat | |
| DE3725851C2 (OSRAM) | ||
| DE69128873T2 (de) | Verfahren zur enzymatischen Messung von Wasserstoffperoxid und Reagenz dafür | |
| DD201734A5 (de) | Verfahren und reagens zur bestimmung von glycerin | |
| DE2929530C2 (de) | Glycerin oxidierendes Enzym, Verfahren zu seiner Herstellung und seine Verwendung zur quantitativen Bestimmung von Glycerin | |
| DE3536662A1 (de) | Mikrobiologisch hergestellte d(-)-mandelat-dehydrogenase, verfahren zu ihrer gewinnung und ihre verwendung | |
| DE3991428C2 (de) | Gallensäuresulfatsulfatase, Verfahren zu ihrer Herstellung und Verfahren zur Gehaltsbestimmung von Gallensäure | |
| EP0089606B1 (de) | Verfahren und Reagenz zur Bestimmung von Glycerin | |
| Haeckel et al. | A New Enzymatic Determination of Cholesterol. The Use of Aldehyde Dehydrogenase to Measure H2O2 Producing Reactions, II. | |
| DE2954471C2 (OSRAM) | ||
| DE2506712A1 (de) | Verfahren zur bestimmung von cholesterin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PL | Patent ceased | ||
| PL | Patent ceased |