FR2359850A1 - Nicotinamide-adenine-dinucleotide reduite (nadh) peroxydase, son obtention et son utilisation - Google Patents
Nicotinamide-adenine-dinucleotide reduite (nadh) peroxydase, son obtention et son utilisationInfo
- Publication number
- FR2359850A1 FR2359850A1 FR7723161A FR7723161A FR2359850A1 FR 2359850 A1 FR2359850 A1 FR 2359850A1 FR 7723161 A FR7723161 A FR 7723161A FR 7723161 A FR7723161 A FR 7723161A FR 2359850 A1 FR2359850 A1 FR 2359850A1
- Authority
- FR
- France
- Prior art keywords
- nadh
- peroxidase
- nicotinamide
- adenine
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 title abstract 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 title abstract 3
- 102000003992 Peroxidases Human genes 0.000 title 1
- 108040007629 peroxidase activity proteins Proteins 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 abstract 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract 2
- 238000005859 coupling reaction Methods 0.000 abstract 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 abstract 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 abstract 2
- 241000194032 Enterococcus faecalis Species 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 abstract 1
- 108010084238 NAD+ peroxidase Proteins 0.000 abstract 1
- 239000007983 Tris buffer Substances 0.000 abstract 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 abstract 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 abstract 1
- 150000001298 alcohols Chemical class 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 235000012000 cholesterol Nutrition 0.000 abstract 1
- 150000002148 esters Chemical class 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 235000011056 potassium acetate Nutrition 0.000 abstract 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 abstract 1
- 229940116269 uric acid Drugs 0.000 abstract 1
- 229940075420 xanthine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
L'invention a pour objet une nouvelle NADH-peroxydase extraite de Streptococcus faecalis ATCC 8043. Cette nouvelle enzyme qui oxyde la NADH au moyen de H2 O2 en NAD et H2 O est caractérisée par une constante de Michaelis KM par rapport à H2 O2 de 2,8. 10**-5 M et par rapport à NADH de 1,7.10**-5 M, mesurées à 25 degrés C dans un tampon TRIS 0,2 M à pH 6,0, renfermant 0, 1M d'acétate de potassium. Cette enzyme sert au dosage de H 2 O2 selon une réaction unique ou couplée ou au dosage du glucose, des aminoacides de types D et L, du cholestérol et des esters, des alcools, de l'acide urique, de la xanthine et de l'hypoxanthine selon des réactions couplées en présence d'autres enzymes de types appropriés.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2633728A DE2633728C3 (de) | 1976-07-27 | 1976-07-27 | NADH-Peroxidase, ihre Gewinnung und Verwendung |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2359850A1 true FR2359850A1 (fr) | 1978-02-24 |
| FR2359850B1 FR2359850B1 (fr) | 1980-04-18 |
Family
ID=5984055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7723161A Granted FR2359850A1 (fr) | 1976-07-27 | 1977-07-27 | Nicotinamide-adenine-dinucleotide reduite (nadh) peroxydase, son obtention et son utilisation |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4186052A (fr) |
| JP (1) | JPS5814198B2 (fr) |
| CH (1) | CH636904A5 (fr) |
| DE (1) | DE2633728C3 (fr) |
| FI (1) | FI56845C (fr) |
| FR (1) | FR2359850A1 (fr) |
| GB (1) | GB1528309A (fr) |
| IT (1) | IT1081816B (fr) |
| NL (1) | NL188045C (fr) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2418239A1 (fr) * | 1978-02-27 | 1979-09-21 | Yamasa Shoyu Kk | L-lysine a-oxydase, sa preparation et son emploi pour le dosage de la l-lysine et comme agent therapeutique |
| EP0007176A2 (fr) * | 1978-06-12 | 1980-01-23 | Cetus Corporation | Procédé de production d'époxydes et de glycols à partir d'alcènes |
| EP0016845B1 (fr) * | 1978-08-18 | 1983-07-13 | Kyowa Hakko Kogyo Co., Ltd. | Methode et test pour la determination d'un substrat de xanthine-oxidase au moyen d'une nouvelle xanthine-oxidase et procede pour sa preparation |
| FR2567906A1 (fr) * | 1984-07-23 | 1986-01-24 | Toyo Jozo Kk | Procede de dosage enzymatique tres sensible |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2806371A1 (de) * | 1978-02-10 | 1979-08-23 | Guenther Kohlbecker | Verfahren zur enzymatischen bestimmung von oxalat mittels oxalat-oxidase |
| US4341868A (en) | 1978-08-18 | 1982-07-27 | Kyowa Hakko Kogyo Co., Ltd. | Method and test composition for the determination of the substrate for xanthine oxidase |
| DE3477812D1 (en) * | 1983-01-12 | 1989-05-24 | Alcoholism & Drug Addiction | Rapid analysis of ethanol in body fluids |
| DE3313178A1 (de) * | 1983-04-12 | 1984-10-18 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur enzymatischen bestimmung von sulfit |
| JPS59205999A (ja) * | 1983-05-10 | 1984-11-21 | Wako Pure Chem Ind Ltd | 還元型補酵素の定量方法 |
| JPS62140999A (ja) * | 1985-12-16 | 1987-06-24 | 川鉄機材工業株式会社 | 重量物懸吊装置 |
| DE3725851A1 (de) * | 1986-08-12 | 1988-02-18 | Takara Shuzo Co | Nad(p)h-oxidase, verfahren zu ihrer isolierung und anwendung |
| US5385827A (en) * | 1989-09-20 | 1995-01-31 | Clark; John R. | Method of geochemical prospecting |
| CA2965481C (fr) * | 2014-10-31 | 2023-03-21 | Rohm And Haas Company | Procede d'esterification oxydative pour la fabrication de methacrylate de methyle |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT986838B (it) * | 1972-05-12 | 1975-01-30 | Sclavo Inst Sieroterapeut | Complesso di reagenti per la deter minazione enzimatica del glucosio sistema glucosio ossidasi perossi dasi con metodo manuale e automati co con lettura a termine o in cinetica |
-
1976
- 1976-07-27 DE DE2633728A patent/DE2633728C3/de not_active Expired
-
1977
- 1977-06-07 IT IT24454/77A patent/IT1081816B/it active
- 1977-06-09 NL NLAANVRAGE7706337,A patent/NL188045C/xx not_active IP Right Cessation
- 1977-06-29 US US05/811,422 patent/US4186052A/en not_active Expired - Lifetime
- 1977-07-13 FI FI772186A patent/FI56845C/fi not_active IP Right Cessation
- 1977-07-22 CH CH913877A patent/CH636904A5/de not_active IP Right Cessation
- 1977-07-25 GB GB31143/77A patent/GB1528309A/en not_active Expired
- 1977-07-27 JP JP52090216A patent/JPS5814198B2/ja not_active Expired
- 1977-07-27 FR FR7723161A patent/FR2359850A1/fr active Granted
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2418239A1 (fr) * | 1978-02-27 | 1979-09-21 | Yamasa Shoyu Kk | L-lysine a-oxydase, sa preparation et son emploi pour le dosage de la l-lysine et comme agent therapeutique |
| EP0007176A2 (fr) * | 1978-06-12 | 1980-01-23 | Cetus Corporation | Procédé de production d'époxydes et de glycols à partir d'alcènes |
| EP0007176A3 (fr) * | 1978-06-12 | 1980-02-20 | Cetus Corporation | Procédé de production d'époxydes et de glycols à partir d'alcènes |
| EP0016845B1 (fr) * | 1978-08-18 | 1983-07-13 | Kyowa Hakko Kogyo Co., Ltd. | Methode et test pour la determination d'un substrat de xanthine-oxidase au moyen d'une nouvelle xanthine-oxidase et procede pour sa preparation |
| FR2567906A1 (fr) * | 1984-07-23 | 1986-01-24 | Toyo Jozo Kk | Procede de dosage enzymatique tres sensible |
Also Published As
| Publication number | Publication date |
|---|---|
| FI56845B (fi) | 1979-12-31 |
| DE2633728B2 (de) | 1978-10-05 |
| FR2359850B1 (fr) | 1980-04-18 |
| CH636904A5 (de) | 1983-06-30 |
| DE2633728C3 (de) | 1979-07-12 |
| FI56845C (fi) | 1980-04-10 |
| JPS5814198B2 (ja) | 1983-03-17 |
| DE2633728A1 (de) | 1978-02-02 |
| NL7706337A (nl) | 1978-01-31 |
| US4186052A (en) | 1980-01-29 |
| NL188045C (nl) | 1992-03-16 |
| JPS5315492A (en) | 1978-02-13 |
| FI772186A (fr) | 1978-01-28 |
| GB1528309A (en) | 1978-10-11 |
| IT1081816B (it) | 1985-05-21 |
| NL188045B (nl) | 1991-10-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |