GB1479994A - Single reagent for the enzymatic determination of cholesterol and method therefor - Google Patents

Single reagent for the enzymatic determination of cholesterol and method therefor

Info

Publication number
GB1479994A
GB1479994A GB7904/75A GB790475A GB1479994A GB 1479994 A GB1479994 A GB 1479994A GB 7904/75 A GB7904/75 A GB 7904/75A GB 790475 A GB790475 A GB 790475A GB 1479994 A GB1479994 A GB 1479994A
Authority
GB
United Kingdom
Prior art keywords
cholesterol
hydrogen
hrp
colour
methyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB7904/75A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of GB1479994A publication Critical patent/GB1479994A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Abstract

1479994 Determination of total cholesterol ABBOTT LABORATORIES 25 Feb 1975 [4 March 1974] 7904/75 Heading G1B Total cholesterol is enzymatically determined in a serum sample using a single reagent comprising a substantially anhydrous mixture of cholesterol esterase, cholesterol oxidase, horse-radish peroxidase (HRP), a hydrogen donor oxidisable by hyrdogen peroxide to produce a colour, and cholic acid, a conjugate thereof or a salt of either, the cholesterol esters in the serum sample being hydrolyzed to free cholesterol which is then oxidized to # 4- cholestenone and hydrogen peroxide, the hydrogen peroxide in the presence of HRP oxidizing the hydrogen donor which may be o-diamisidine or 4-aminoantipyrine + phenol to form a colour which is measured. The assay of cholesterol oxidase by conversion of cholesterol to cholest-4-en-3-one and measurement of the latter at 240 nm is disclosed. Hydrogen donors other than those mentioned above are 4', 4', 4" methylidynetris (N,N-dimethylaniline), 3-methyl-2-benzo-thiazolinone hydrazone/o-toluidine, 3-methyl-2-benzothiazolinone hydrazone/ N,N-dimethyl aniline, 2, 2' -azino-di-(3-ethylbenzothiazolin sulfonate-6) diammonium salt.
GB7904/75A 1974-03-04 1975-02-25 Single reagent for the enzymatic determination of cholesterol and method therefor Expired GB1479994A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44769174A 1974-03-04 1974-03-04

Publications (1)

Publication Number Publication Date
GB1479994A true GB1479994A (en) 1977-07-13

Family

ID=23777351

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7904/75A Expired GB1479994A (en) 1974-03-04 1975-02-25 Single reagent for the enzymatic determination of cholesterol and method therefor

Country Status (5)

Country Link
JP (1) JPS50125796A (en)
DE (1) DE2509156A1 (en)
FR (1) FR2263516A1 (en)
GB (1) GB1479994A (en)
SE (1) SE7502372L (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0024578A1 (en) * 1979-08-23 1981-03-11 Ivan Endre Modrovich Method of stabilizing an enzyme solution for use in total cholesterol determination, stabilized solution and test kit therefor
US4275151A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
EP0044432A2 (en) * 1980-07-10 1982-01-27 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
EP0053692A1 (en) * 1980-12-08 1982-06-16 Roche Diagnostics GmbH Process and reagent for determining cholesterol
EP0091026A2 (en) * 1982-04-02 1983-10-12 Ivan Endre Modrovich Improved time-stable liquid cholesterol assay compositions
US5047327A (en) * 1982-04-02 1991-09-10 Ivan E. Modrovich Time-stable liquid cholesterol assay compositions

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1104041A (en) * 1977-02-03 1981-06-30 Charles T. Goodhue Hydrolysis of protein-bound cholesterol esters
US4275152A (en) * 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
DE2856487A1 (en) * 1977-12-29 1979-07-12 Wako Pure Chem Ind Ltd PROCEDURE FOR DETERMINING PEROXID SUBSTANCES AND COMPOUNDS THAT CAN BE USED THEREFORE
JPS5520471A (en) * 1978-08-01 1980-02-13 Kyowa Hakko Kogyo Co Ltd Hydrogen peroxide quantifying method
DE2911284C2 (en) * 1979-03-22 1982-01-28 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for activating cholesterol esterase

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275151A (en) 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
EP0024578A1 (en) * 1979-08-23 1981-03-11 Ivan Endre Modrovich Method of stabilizing an enzyme solution for use in total cholesterol determination, stabilized solution and test kit therefor
EP0044432A2 (en) * 1980-07-10 1982-01-27 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
EP0044432A3 (en) * 1980-07-10 1982-02-03 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
EP0053692A1 (en) * 1980-12-08 1982-06-16 Roche Diagnostics GmbH Process and reagent for determining cholesterol
EP0091026A2 (en) * 1982-04-02 1983-10-12 Ivan Endre Modrovich Improved time-stable liquid cholesterol assay compositions
EP0091026A3 (en) * 1982-04-02 1985-07-10 Ivan Endre Modrovich Improved time-stable liquid cholesterol assay compositions
US5047327A (en) * 1982-04-02 1991-09-10 Ivan E. Modrovich Time-stable liquid cholesterol assay compositions

Also Published As

Publication number Publication date
JPS50125796A (en) 1975-10-03
DE2509156A1 (en) 1975-09-25
FR2263516A1 (en) 1975-10-03
SE7502372L (en) 1975-09-05

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Legal Events

Date Code Title Description
414F Notice of opposition given (sect. 14/1949)
414E Application relinquished (sect. 14/1949)
CSNS Application of which complete specification have been accepted and published, but patent is not sealed