GB1429525A - Process and reagent for the quantitative determination of cholesterol - Google Patents

Process and reagent for the quantitative determination of cholesterol

Info

Publication number
GB1429525A
GB1429525A GB2308273A GB2308273A GB1429525A GB 1429525 A GB1429525 A GB 1429525A GB 2308273 A GB2308273 A GB 2308273A GB 2308273 A GB2308273 A GB 2308273A GB 1429525 A GB1429525 A GB 1429525A
Authority
GB
United Kingdom
Prior art keywords
cholesterol
amount
active agent
reagent
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2308273A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25763268&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=GB1429525(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of GB1429525A publication Critical patent/GB1429525A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1429525 Determination of cholesterol BOEHRINGER MANNHEIM GmbH 15 May 1973 [17 May 1972] 23082/73 Heading G1B Cholesterol in an aqueous medium is quantitatively determined by incubating the medium with cholesterol oxidase and thereafter determining either the amount of oxygen used up or the amount of hydrogen peroxide or of cholestenone formed, on the basis of the reaction. cholesterol oxidase cholesterol + O 2 # cholestonone + H 2 O 2 (A) The amount of oxygen used up may be measured polarometrically, (B) the amount of hydrogen peroxide formed may be determined enzymatically with the use of catalase or peroxidase, (C) the amount of cholestenone is measured directly by direct absorption spectrophotometry or with the use of a Keto group-reacting compound. Bound or total cholesterol is determined by testing a sample in which the free cholesterol concentration has already been tested or a separate, untested sample, respectively, after such samples have been saponified using alcoholic potassium hydroxide solution or cholesterol esterase. Reagent Kito containing specified proportions of cholesterol oxidase and the following constituents are disclosed: (A) surface-active agent (hydroxpolyethoxydodecane) in phosphate buffer, (B) catalase, acetylacetone, methanol surface-active agent and buffer OR peroxidase, a chromogen (2, 2 -aminobenzthiazoline-sulphonic acid), surface-active agent and buffer, (C) Keto group-reacting compound (2, 4-dinitro-phenylhydrozine and surface-active agent. A reagent for saponifying esterified cholesterol (as mentioned above) may also be included.
GB2308273A 1972-05-17 1973-05-15 Process and reagent for the quantitative determination of cholesterol Expired GB1429525A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19722224132 DE2224132C2 (en) 1972-05-17 1972-05-17 Method and reagent for the determination of cholesterol
DE2264847A DE2264847C2 (en) 1972-05-17 1972-05-17 Method and reagent for the determination of cholesterol

Publications (1)

Publication Number Publication Date
GB1429525A true GB1429525A (en) 1976-03-24

Family

ID=25763268

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2308273A Expired GB1429525A (en) 1972-05-17 1973-05-15 Process and reagent for the quantitative determination of cholesterol

Country Status (7)

Country Link
AT (1) AT324580B (en)
BE (1) BE799543A (en)
CH (1) CH589295A5 (en)
DE (2) DE2264847C2 (en)
FR (1) FR2191743A5 (en)
GB (1) GB1429525A (en)
NL (1) NL181282C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0044432A2 (en) * 1980-07-10 1982-01-27 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
US5047327A (en) * 1982-04-02 1991-09-10 Ivan E. Modrovich Time-stable liquid cholesterol assay compositions
WO2011061304A1 (en) * 2009-11-20 2011-05-26 Pharnext New diagnostic tools for charcot-marie-tooth disease

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4186251A (en) * 1973-03-01 1980-01-29 Miles Laboratories, Inc. Composition and method for determination of cholesterol
US4164448A (en) * 1973-12-07 1979-08-14 Boehringer Mannheim Gmbh Activation of cholesterol oxidase for cholesterol assay
US3869349A (en) * 1974-03-25 1975-03-04 Eastman Kodak Co Method for the enzymatic hydrolysis of cholesterol esters
US3884764A (en) * 1974-03-25 1975-05-20 Eastman Kodak Co Method and composition for blood serum cholesterol analysis
CA1056282A (en) * 1974-03-25 1979-06-12 Charles T. Goodhue Multilayer analytical elements for use in the assay of cholesterol
US4116773A (en) * 1976-07-01 1978-09-26 Beckman Instruments, Inc. Enzymatic reagent and method for increasing the sensitivity of enzymatic substrate analyses using oxygen rate analyzers
DE2649749A1 (en) * 1976-10-29 1978-05-11 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR THE DETERMINATION OF TOTAL CHOLESTEROL OR BONDED CHOLESTEROL
DE2653537C3 (en) 1976-11-25 1979-08-23 Merck Patent Gmbh, 6100 Darmstadt Method and means for the determination of hydroperoxides

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1422881A (en) * 1963-08-16 1966-01-03 Warner Lambert Pharmaceutical Auxiliary diagnostic composition for use in determining cholesterol
NL137003C (en) * 1967-10-14
FR2047147A5 (en) * 1969-05-06 1971-03-12 Kyowa Hakko Kogyo Kk
DE2130340C3 (en) * 1971-06-18 1975-07-03 Boehringer Mannheim Gmbh, 6800 Mannheim Method and device for the quantitative determination of individual samples of different concentrations of substances
GB1385319A (en) * 1971-09-22 1975-02-26 Nat Res Dev Enzyme preparations

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0044432A2 (en) * 1980-07-10 1982-01-27 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
EP0044432A3 (en) * 1980-07-10 1982-02-03 Ivan Endre Modrovich Stabilized enzymatic solutions and method for determining total cholesterol in human serum
US5047327A (en) * 1982-04-02 1991-09-10 Ivan E. Modrovich Time-stable liquid cholesterol assay compositions
WO2011061304A1 (en) * 2009-11-20 2011-05-26 Pharnext New diagnostic tools for charcot-marie-tooth disease
US9494605B2 (en) 2009-11-20 2016-11-15 Pharnext Diagnostic tools for charcot-marie-tooth disease
US9945876B2 (en) 2009-11-20 2018-04-17 Pharnext Diagnostic tools for charcot-marie-tooth disease

Also Published As

Publication number Publication date
DE2224132B1 (en) 1973-11-22
FR2191743A5 (en) 1974-02-01
NL181282C (en) 1987-07-16
NL7306738A (en) 1973-11-20
DE2224132C2 (en) 1980-04-03
DE2224132A1 (en) 1973-11-22
DE2264847A1 (en) 1975-05-28
AT324580B (en) 1975-09-10
DE2264847C2 (en) 1978-11-30
CH589295A5 (en) 1977-06-30
BE799543A (en) 1973-11-16
AU5586173A (en) 1974-11-21
NL181282B (en) 1987-02-16

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Legal Events

Date Code Title Description
48R Reference inserted (sect. 8/1949)
414F Notice of opposition given (sect. 14/1949)
414A Case decided by the comptroller ** specification amended (sect. 14/1949)
PS Patent sealed
SP Amendment (slips) printed
PE20 Patent expired after termination of 20 years

Effective date: 19930514