CH635615A5 - Process for decreasing the lipid and nucleic acid content in microbial cell masses - Google Patents
Process for decreasing the lipid and nucleic acid content in microbial cell masses Download PDFInfo
- Publication number
- CH635615A5 CH635615A5 CH913977A CH913977A CH635615A5 CH 635615 A5 CH635615 A5 CH 635615A5 CH 913977 A CH913977 A CH 913977A CH 913977 A CH913977 A CH 913977A CH 635615 A5 CH635615 A5 CH 635615A5
- Authority
- CH
- Switzerland
- Prior art keywords
- solvent
- nucleic acid
- cell mass
- methanol
- cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 29
- 150000002632 lipids Chemical class 0.000 title claims description 21
- 150000007523 nucleic acids Chemical class 0.000 title claims description 21
- 102000039446 nucleic acids Human genes 0.000 title claims description 21
- 108020004707 nucleic acids Proteins 0.000 title claims description 21
- 230000000813 microbial effect Effects 0.000 title claims description 8
- 230000003247 decreasing effect Effects 0.000 title 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 239000002904 solvent Substances 0.000 claims description 24
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 17
- 238000000605 extraction Methods 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- 229910021529 ammonia Inorganic materials 0.000 claims description 13
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 10
- 239000003960 organic solvent Substances 0.000 claims description 9
- 230000029087 digestion Effects 0.000 claims description 7
- 239000008346 aqueous phase Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000000908 ammonium hydroxide Substances 0.000 claims description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 2
- 150000002431 hydrogen Chemical group 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 81
- 210000004027 cell Anatomy 0.000 description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 23
- 235000011114 ammonium hydroxide Nutrition 0.000 description 15
- 239000000463 material Substances 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000001914 filtration Methods 0.000 description 7
- 239000007858 starting material Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 239000007789 gas Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000013049 sediment Substances 0.000 description 5
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 239000003925 fat Substances 0.000 description 4
- 239000007791 liquid phase Substances 0.000 description 4
- 230000009965 odorless effect Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 210000005253 yeast cell Anatomy 0.000 description 3
- XNWFRZJHXBZDAG-UHFFFAOYSA-N 2-METHOXYETHANOL Chemical compound COCCO XNWFRZJHXBZDAG-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000589344 Methylomonas Species 0.000 description 2
- 241000589341 Methylomonas clara Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 241000235015 Yarrowia lipolytica Species 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000021588 free fatty acids Nutrition 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000108056 Monas Species 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 208000009911 Urinary Calculi Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 1
- 229940060799 clarus Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 208000008281 urolithiasis Diseases 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Extraction Or Liquid Replacement (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2633666A DE2633666C3 (de) | 1976-07-27 | 1976-07-27 | Verminderung des Lipid- und Nucleinsäure-Gehaltes in mikrobiellen Zellmassen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH635615A5 true CH635615A5 (en) | 1983-04-15 |
Family
ID=5984032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH913977A CH635615A5 (en) | 1976-07-27 | 1977-07-22 | Process for decreasing the lipid and nucleic acid content in microbial cell masses |
Country Status (28)
| Country | Link |
|---|---|
| JP (1) | JPS5315489A (fr) |
| AT (1) | AT357502B (fr) |
| AU (1) | AU512670B2 (fr) |
| BE (1) | BE857229A (fr) |
| BG (1) | BG37997A3 (fr) |
| CA (1) | CA1101724A (fr) |
| CH (1) | CH635615A5 (fr) |
| CS (1) | CS207473B2 (fr) |
| DD (1) | DD131072A5 (fr) |
| DE (1) | DE2633666C3 (fr) |
| DK (1) | DK146511C (fr) |
| EG (1) | EG12626A (fr) |
| ES (1) | ES460877A1 (fr) |
| FI (1) | FI57443C (fr) |
| FR (1) | FR2359896A1 (fr) |
| GB (1) | GB1584194A (fr) |
| GR (1) | GR64056B (fr) |
| HU (1) | HU176802B (fr) |
| IL (1) | IL52594A (fr) |
| IT (1) | IT1082226B (fr) |
| NL (1) | NL7708171A (fr) |
| NO (1) | NO147034C (fr) |
| PL (1) | PL107949B1 (fr) |
| PT (1) | PT66849B (fr) |
| RO (1) | RO71982A (fr) |
| SE (1) | SE432609B (fr) |
| SU (1) | SU791257A3 (fr) |
| ZA (1) | ZA774517B (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996038535A1 (fr) * | 1995-05-29 | 1996-12-05 | Nauchno-Issledovatelsky Institut Vychislitelnykh Komplexov | Procede d'obtention d'une biomasse de micro-organismes ayant un faible contenu en acides nucleiques |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2748885A1 (de) * | 1977-11-02 | 1979-05-03 | Hoechst Ag | Verfahren zur verbesserung der eigenschaften von schroten oder mehlen aus oelsaaten |
| DE2907065A1 (de) | 1979-02-23 | 1980-09-04 | Hoechst Ag | Verfahren zum fetten von leder und pelzfellen |
| CA1120314A (fr) * | 1979-08-22 | 1982-03-23 | John A. Ridgway | Traitement de substances proteiniques au gaz ammoniac anhydre |
| DE3143947A1 (de) * | 1981-11-05 | 1983-05-11 | Hoechst Ag, 6230 Frankfurt | "funktionelle eiweisshydrolysate, verfahren zu ihrer herstellung und diese eiweisshydrolysate enthaltende nahrungsmittel" |
| DE3228500A1 (de) * | 1982-07-30 | 1984-02-02 | Basf Ag, 6700 Ludwigshafen | Verfahren zur reinigung von aldehyde, acetale und/oder ungesaettigte verbindungen enthaltenden carbonsaeureestern |
| RU2522888C2 (ru) * | 2012-10-04 | 2014-07-20 | Валентина Еремеевна Куцакова | Способ получения белковой кормовой добавки |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3615654A (en) * | 1968-05-17 | 1971-10-26 | Cpc International Inc | Method of treating microbial cells |
| IT954172B (it) * | 1970-07-24 | 1973-08-30 | Standard Oil Co | Procedimento per estrarre materiale proteico da organismi microbici unicellulari e prodotto ottenuto |
| SU419551A1 (ru) * | 1972-01-18 | 1974-03-15 | В. Ф. Фоменков Институт биологической физики СССР | Тепловой расходомер |
| GB1400691A (en) * | 1973-02-08 | 1975-07-23 | British Petroleum Co | Process for the production of proteinaceous material |
| GB1408845A (en) * | 1973-02-13 | 1975-10-08 | Ranks Hovis Mcdougall Ltd | Production of edible protein containing substances |
| JPS5411379B2 (fr) * | 1973-02-21 | 1979-05-15 |
-
1976
- 1976-07-27 DE DE2633666A patent/DE2633666C3/de not_active Expired
-
1977
- 1977-07-20 ES ES460877A patent/ES460877A1/es not_active Expired
- 1977-07-22 BG BG036986A patent/BG37997A3/xx unknown
- 1977-07-22 NL NL7708171A patent/NL7708171A/xx not_active Application Discontinuation
- 1977-07-22 CH CH913977A patent/CH635615A5/de not_active IP Right Cessation
- 1977-07-25 GR GR54033A patent/GR64056B/el unknown
- 1977-07-25 SE SE7708539A patent/SE432609B/xx not_active IP Right Cessation
- 1977-07-25 RO RO7791163A patent/RO71982A/fr unknown
- 1977-07-25 EG EG439/77A patent/EG12626A/xx active
- 1977-07-25 IL IL52594A patent/IL52594A/xx unknown
- 1977-07-25 IT IT26084/77A patent/IT1082226B/it active
- 1977-07-25 DD DD7700200262A patent/DD131072A5/xx unknown
- 1977-07-25 FI FI772272A patent/FI57443C/fi not_active IP Right Cessation
- 1977-07-26 PT PT66849A patent/PT66849B/pt unknown
- 1977-07-26 PL PL1977199855A patent/PL107949B1/pl unknown
- 1977-07-26 CA CA283,487A patent/CA1101724A/fr not_active Expired
- 1977-07-26 NO NO772658A patent/NO147034C/no unknown
- 1977-07-26 SU SU772504953A patent/SU791257A3/ru active
- 1977-07-26 HU HU77HO2006A patent/HU176802B/hu unknown
- 1977-07-26 AU AU27319/77A patent/AU512670B2/en not_active Expired
- 1977-07-26 DK DK337677A patent/DK146511C/da not_active IP Right Cessation
- 1977-07-26 AT AT544077A patent/AT357502B/de not_active IP Right Cessation
- 1977-07-26 ZA ZA00774517A patent/ZA774517B/xx unknown
- 1977-07-27 GB GB31537/77A patent/GB1584194A/en not_active Expired
- 1977-07-27 JP JP8934977A patent/JPS5315489A/ja active Granted
- 1977-07-27 BE BE179698A patent/BE857229A/fr not_active IP Right Cessation
- 1977-07-27 FR FR7723082A patent/FR2359896A1/fr active Granted
- 1977-07-27 CS CS764982A patent/CS207473B2/cs unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996038535A1 (fr) * | 1995-05-29 | 1996-12-05 | Nauchno-Issledovatelsky Institut Vychislitelnykh Komplexov | Procede d'obtention d'une biomasse de micro-organismes ayant un faible contenu en acides nucleiques |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PL | Patent ceased | ||
| PL | Patent ceased |