CA3127994A1 - L-tyrosine-producing microorganism and method for producing l-tyrosine using the same - Google Patents
L-tyrosine-producing microorganism and method for producing l-tyrosine using the same Download PDFInfo
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- CA3127994A1 CA3127994A1 CA3127994A CA3127994A CA3127994A1 CA 3127994 A1 CA3127994 A1 CA 3127994A1 CA 3127994 A CA3127994 A CA 3127994A CA 3127994 A CA3127994 A CA 3127994A CA 3127994 A1 CA3127994 A1 CA 3127994A1
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| US7482140B2 (en) * | 2004-06-15 | 2009-01-27 | Ajinomoto Co., Inc. | L-tyrosine-producing bacterium and a method for producing L-tyrosine |
| JP4665613B2 (ja) * | 2004-06-15 | 2011-04-06 | 味の素株式会社 | Ｌ−チロシン生産菌及びｌ−チロシンの製造法 |
| CN103642882A (zh) * | 2004-06-25 | 2014-03-19 | 协和发酵生化株式会社 | 二肽的制造方法 |
| KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
| KR100653742B1 (ko) * | 2004-12-30 | 2006-12-05 | 씨제이 주식회사 | 신규한 ｌ-라이신-유도성 프로모터 |
| DE102005019040A1 (de) * | 2005-04-23 | 2006-10-26 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung verbesserter Stämme der Familie Enterobacteriaceae |
| US7700328B2 (en) | 2006-06-07 | 2010-04-20 | E.I. Du Pont De Nemours And Company | Method for producing an L-tyrosine over-producing bacterial strain |
| JP5756259B2 (ja) | 2006-09-15 | 2015-07-29 | シージェイ チェイルジェダン コーポレイション | Ｌ−リジン生産能の向上したコリネバクテリウム属およびそれを用いたｌ−リジン生産方法 |
| US20080102499A1 (en) * | 2006-10-27 | 2008-05-01 | Lori Jean Templeton | Method of enhancing L-tyrosine production in recombinant bacteria |
| KR100792095B1 (ko) * | 2006-12-29 | 2008-01-04 | 씨제이 주식회사 | Ｌ-페닐알라닌 생산능을 갖는 대장균 변이주로부터유전자조작된 ｌ-트립토판 생산능을 갖는 재조합 대장균균주 및 이를 이용한 트립토판 제조방법 |
| EP2336347B1 (en) * | 2008-09-08 | 2017-03-15 | Ajinomoto Co., Inc. | An l-amino acid-producing microorganism and a method for producing an l-amino acid |
| EP2395096B1 (en) * | 2009-02-09 | 2014-04-09 | Kyowa Hakko Bio Co., Ltd. | Process for producing L-amino acids |
| US8283152B2 (en) * | 2009-08-28 | 2012-10-09 | Cj Cheiljedang Corporation | Microorganism producing O-acetyl-homoserine and the method of producing O-acetyl-homoserine using the microorganism |
| CN102399835A (zh) * | 2011-10-14 | 2012-04-04 | 江南大学 | 一种微生物发酵生产l-苯丙氨酸的方法 |
| KR101327093B1 (ko) * | 2012-01-06 | 2013-11-07 | 씨제이제일제당 (주) | Ｌ-아미노산을 생산할 수 있는 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법 |
| RU2593957C2 (ru) * | 2012-01-10 | 2016-08-10 | СиДжей ЧЕИЛДЗЕДАНГ КОРПОРЕЙШН | МИКРООРГАНИЗМ ИЗ РОДА Escherichia, ОБЛАДАЮЩИЙ УСИЛЕННОЙ СПОСОБНОСТЬЮ К ПРОДУКЦИИ L-ТРИПТОФАНА, И СПОСОБ ПОЛУЧЕНИЯ L-ТРИПТОФАНА С ЕГО ИСПОЛЬЗОВАНИЕМ |
| EP2628792A1 (de) * | 2012-02-17 | 2013-08-21 | Evonik Industries AG | Zelle mit verringerter ppGppase-Aktivität |
| CN103074292B (zh) * | 2013-01-22 | 2014-07-09 | 江南大学 | 一种高产l-苯丙氨酸的重组谷氨酸棒杆菌及其应用 |
| KR101721722B1 (ko) * | 2013-03-11 | 2017-04-10 | 씨제이제일제당 (주) | L-발린 생산능이 향상된 균주 및 이를 이용한 l-발린 생산방법 |
| ES2643442T3 (es) * | 2013-04-16 | 2017-11-22 | Cj Cheiljedang Corporation | Microorganismo que presenta productividad de L-triptófano y método para la producción del L-triptófano mediante la utilización del mismo |
| JP3185261U (ja) * | 2013-05-29 | 2013-08-08 | 広太 小西 | 花束保持具 |
| DK2811028T3 (en) * | 2013-06-03 | 2017-05-01 | Evonik Degussa Gmbh | Process for Preparation of L-Valine Using Recombinant Coryn Bacteria Containing the Propionate Inducible IlvBN Operon |
| KR101518860B1 (ko) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | L-아미노산의 생산 방법 |
| KR101599802B1 (ko) * | 2014-05-23 | 2016-03-04 | 씨제이제일제당 주식회사 | 세포내 에너지 수준이 향상된 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법 |
| KR101835173B1 (ko) * | 2014-06-23 | 2018-03-07 | 씨제이제일제당 (주) | L-트립토판 생산능을 갖는 에스케리키아속 미생물 및 이를 이용한 l-트립토판의 제조 방법 |
| KR101835935B1 (ko) * | 2014-10-13 | 2018-03-12 | 씨제이제일제당 (주) | L-아르기닌을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아르기닌의 제조 방법 |
| ES2932086T3 (es) * | 2016-12-28 | 2023-01-11 | Cj Cheiljedang Corp | Una variante novedosa de la isopropilmalato sintasa y un método para producir L-leucina mediante el uso de la misma |
| CN109536428B (zh) * | 2018-12-07 | 2022-08-30 | 武汉远大弘元股份有限公司 | 一种产l-异亮氨酸的基因工程菌及其构建方法和应用 |
| CN109628365A (zh) * | 2019-01-09 | 2019-04-16 | 江南大学 | 一种可提高大肠杆菌酪氨酸产量的方法 |
| CN109554325B (zh) * | 2019-01-09 | 2020-11-03 | 江南大学 | 一种可高产酪氨酸的大肠杆菌工程菌及其应用 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4116317A4 (en) * | 2021-05-20 | 2024-07-03 | Cj Cheiljedang Corp | NEW PROMOTER AND ITS USE |
Also Published As
| Publication number | Publication date |
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| JP7267441B2 (ja) | 2023-05-01 |
| CN114072492A (zh) | 2022-02-18 |
| EP3901265A4 (en) | 2022-03-30 |
| JP2022521384A (ja) | 2022-04-07 |
| BR112021015080A2 (US06244707-20010612-C00010.png) | 2021-12-28 |
| AR119176A1 (es) | 2021-12-01 |
| US20220195470A1 (en) | 2022-06-23 |
| AU2020295264A1 (en) | 2021-08-12 |
| CN114072492B (zh) | 2024-04-30 |
| WO2020256415A1 (ko) | 2020-12-24 |
| EP3901265A1 (en) | 2021-10-27 |
| AU2020295264B2 (en) | 2023-05-18 |
| KR102134418B1 (ko) | 2020-07-16 |
| BR112021015080B1 (pt) | 2024-01-09 |
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