CA2938722A1 - In vitro prediction of in vivo half-life of antibodies - Google Patents

In vitro prediction of in vivo half-life of antibodies Download PDF

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Publication number
CA2938722A1
CA2938722A1 CA2938722A CA2938722A CA2938722A1 CA 2938722 A1 CA2938722 A1 CA 2938722A1 CA 2938722 A CA2938722 A CA 2938722A CA 2938722 A CA2938722 A CA 2938722A CA 2938722 A1 CA2938722 A1 CA 2938722A1
Authority
CA
Canada
Prior art keywords
antibody
retention time
fcrn
region
life
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2938722A
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English (en)
French (fr)
Inventor
Thomas Emrich
Hubert Kettenberger
Tilman Schlothauer
Angela SCHOCH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2938722A1 publication Critical patent/CA2938722A1/en
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B01D15/168—Fluid composition conditioning, e.g. gradient pH gradient or chromatofocusing, i.e. separation according to the isoelectric point pI
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2938722A 2014-03-21 2015-03-17 In vitro prediction of in vivo half-life of antibodies Abandoned CA2938722A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14161103 2014-03-21
EP14161103.8 2014-03-21
EP14165987 2014-04-25
EP14165987.0 2014-04-25
PCT/EP2015/055482 WO2015140126A1 (en) 2014-03-21 2015-03-17 In vitro prediction of in vivo half-life of antibodies

Publications (1)

Publication Number Publication Date
CA2938722A1 true CA2938722A1 (en) 2015-09-24

Family

ID=52684232

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2938722A Abandoned CA2938722A1 (en) 2014-03-21 2015-03-17 In vitro prediction of in vivo half-life of antibodies

Country Status (10)

Country Link
US (2) US20170227547A1 (enExample)
EP (1) EP3119490B1 (enExample)
JP (2) JP6744855B2 (enExample)
KR (1) KR20160134687A (enExample)
CN (1) CN106103478B (enExample)
BR (1) BR112016023417A2 (enExample)
CA (1) CA2938722A1 (enExample)
MX (1) MX377315B (enExample)
RU (1) RU2688349C2 (enExample)
WO (1) WO2015140126A1 (enExample)

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CN106957364B (zh) * 2016-01-11 2021-03-16 上海交通大学 一种单克隆抗体FnAb12及其应用
CN110573626B (zh) * 2017-04-28 2024-05-03 豪夫迈·罗氏有限公司 抗体选择方法
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EP3861025A1 (en) 2018-10-01 2021-08-11 F. Hoffmann-La Roche AG Bispecific antigen binding molecules with trivalent binding to cd40
CN112955240B (zh) * 2018-10-25 2022-09-16 豪夫迈·罗氏有限公司 抗体FcRn结合的修饰
CN113227134A (zh) * 2018-12-05 2021-08-06 株式会社梅花治疗 抗体的Fc区变体
CN119716045A (zh) 2018-12-30 2025-03-28 豪夫迈·罗氏有限公司 基于pH梯度SPR的结合测定
AR121706A1 (es) 2020-04-01 2022-06-29 Hoffmann La Roche Moléculas de unión a antígeno biespecíficas dirigidas a ox40 y fap
EP4133269A1 (en) * 2020-04-08 2023-02-15 F. Hoffmann-La Roche AG Large molecule unspecific clearance assay
JP7322858B2 (ja) * 2020-11-02 2023-08-08 株式会社三洋物産 遊技機
JP7322857B2 (ja) * 2020-11-02 2023-08-08 株式会社三洋物産 遊技機
WO2022175217A1 (en) * 2021-02-18 2022-08-25 F. Hoffmann-La Roche Ag Method for resolving complex, multistep antibody interactions
CN113308476B (zh) * 2021-05-12 2022-06-28 广东药康生物科技有限公司 一种FcRn基因人源化动物模型的构建方法
EP4342984A4 (en) * 2021-05-19 2025-04-23 Chugai Seiyaku Kabushiki Kaisha Method for predicting in vivo pharmacokinetics of molecule
TW202535933A (zh) * 2023-10-25 2025-09-16 美商多重宇宙製藥公司 靶向ｉｌ－１２及ｉｌ－２３之抗原結合分子
WO2025224705A1 (en) * 2024-04-26 2025-10-30 Waters Technologies Corporation Direct affinity to size-exclusion chromatography methods and systems thereof

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EP3119490B1 (en) 2021-09-08
RU2688349C2 (ru) 2019-05-21
RU2016137932A (ru) 2018-04-23
US20210190795A1 (en) 2021-06-24
JP2020183967A (ja) 2020-11-12
JP6744855B2 (ja) 2020-08-19
US20170227547A1 (en) 2017-08-10
KR20160134687A (ko) 2016-11-23
EP3119490A1 (en) 2017-01-25
CN106103478A (zh) 2016-11-09
RU2016137932A3 (enExample) 2018-10-29
BR112016023417A2 (pt) 2019-04-16
US12461109B2 (en) 2025-11-04
MX377315B (es) 2025-03-07
JP2017517745A (ja) 2017-06-29
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CN106103478B (zh) 2020-04-03
JP7032490B2 (ja) 2022-03-08

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