CA2685326A1 - Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins - Google Patents

Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins Download PDF

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Publication number
CA2685326A1
CA2685326A1 CA002685326A CA2685326A CA2685326A1 CA 2685326 A1 CA2685326 A1 CA 2685326A1 CA 002685326 A CA002685326 A CA 002685326A CA 2685326 A CA2685326 A CA 2685326A CA 2685326 A1 CA2685326 A1 CA 2685326A1
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Canada
Prior art keywords
protein
expression
population
array
interest
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Abandoned
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CA002685326A
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English (en)
French (fr)
Inventor
Thomas M. Ramseier
Russell J. Coleman
Jane C. Schneider
Charles D. Hershberger
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Pelican Technology Holdings Inc
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Individual
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Priority to CA2964910A priority Critical patent/CA2964910C/en
Publication of CA2685326A1 publication Critical patent/CA2685326A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002685326A 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins Abandoned CA2685326A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2964910A CA2964910C (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US91436107P 2007-04-27 2007-04-27
US60/914,361 2007-04-27
PCT/US2008/061483 WO2008134461A2 (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2964910A Division CA2964910C (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Publications (1)

Publication Number Publication Date
CA2685326A1 true CA2685326A1 (en) 2008-11-06

Family

ID=39739280

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2964910A Active CA2964910C (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CA002685326A Abandoned CA2685326A1 (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2964910A Active CA2964910C (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Country Status (10)

Country Link
US (1) US9394571B2 (enExample)
EP (2) EP2615172A1 (enExample)
JP (1) JP5444553B2 (enExample)
KR (1) KR101530395B1 (enExample)
CN (1) CN101688213A (enExample)
AU (1) AU2008245696B2 (enExample)
BR (1) BRPI0810120A2 (enExample)
CA (2) CA2964910C (enExample)
MX (1) MX2009011523A (enExample)
WO (1) WO2008134461A2 (enExample)

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