ATE78515T1 - Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. - Google Patents

Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.

Info

Publication number
ATE78515T1
ATE78515T1 AT85307044T AT85307044T ATE78515T1 AT E78515 T1 ATE78515 T1 AT E78515T1 AT 85307044 T AT85307044 T AT 85307044T AT 85307044 T AT85307044 T AT 85307044T AT E78515 T1 ATE78515 T1 AT E78515T1
Authority
AT
Austria
Prior art keywords
periplasmic
dna encoding
cells
proteins
dna
Prior art date
Application number
AT85307044T
Other languages
English (en)
Inventor
Barry Ronald Bochner
Chung-Nan Chang
Gregory Lawrence Gray
Herbert Louis Heyneker
Nancy Chadwick Mcfarland
Kenneth Charles Olson
Rong-Chang Pai
Michael Willard Rey
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/658,342 external-priority patent/US4963495A/en
Priority claimed from US06/658,339 external-priority patent/US4680262A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE78515T1 publication Critical patent/ATE78515T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AT85307044T 1984-10-05 1985-10-02 Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. ATE78515T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US65809584A 1984-10-05 1984-10-05
US06/658,342 US4963495A (en) 1984-10-05 1984-10-05 Secretion of heterologous proteins
US06/658,339 US4680262A (en) 1984-10-05 1984-10-05 Periplasmic protein recovery
EP85307044A EP0177343B1 (de) 1984-10-05 1985-10-02 DNA, Zellkulturen und Verfahren zur Sekretion von heterologen Proteinen und periplasmische Proteinrückgewinnung

Publications (1)

Publication Number Publication Date
ATE78515T1 true ATE78515T1 (de) 1992-08-15

Family

ID=27417994

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85307044T ATE78515T1 (de) 1984-10-05 1985-10-02 Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.

Country Status (4)

Country Link
EP (1) EP0177343B1 (de)
JP (2) JPH0815440B2 (de)
AT (1) ATE78515T1 (de)
DE (1) DE3586386T2 (de)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6255087A (ja) * 1985-03-25 1987-03-10 シタス　オンコロジー　コーポレイション 融合蛋白質をコ−ドする組換ｄｎａ配列
EP0196864A3 (de) * 1985-03-25 1988-03-23 Cetus Corporation Behandlung und Sekretion von rekombinanten Proteinen mittels alkalischer Phosphatase, dazu verwendbare DNA-Sequenzen und mittels dieser Sequenzen transformierte Zellen
IL78775A (en) * 1985-05-15 1992-06-21 Biotech Australia Pty Ltd Oral vaccines
US6103243A (en) * 1985-05-15 2000-08-15 Biotechnology Australia Pty, Ltd Oral vaccines
IL83003A (en) 1986-07-01 1995-07-31 Genetics Inst Factors that soak bone formation
GR871029B (en) 1986-07-14 1987-11-02 Genetics Inst Novel osteoinductive factors
US4933288A (en) * 1986-11-21 1990-06-12 Cetus Corporation Use of a modified soluble Pseudomonas exotoxin A in immunoconjugates
NZ224241A (en) * 1987-04-14 1991-03-26 Bresatec Ltd Method of producing new animal breeds using plasmid expression vectors; plasmid expression vectors
US5573933A (en) * 1987-04-14 1996-11-12 Luminis Pty, Ltd. Transgenic pigs
DE3716957A1 (de) * 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
FR2636643B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Peptide-signal, sequences d'adn codant pour celui-ci, vecteurs d'expression portant l'une de ces sequences et procede de production periplasmique d'un polypeptide
US5223407A (en) * 1988-08-31 1993-06-29 Allelix Inc. Excretion of heterologous proteins from e. coli
US5646015A (en) * 1988-08-31 1997-07-08 Astra Ab Excretion of heterologous proteins from E. coli
IE910147A1 (en) * 1990-01-18 1991-07-31 Astra Ab A novel vector to produce biologically important peptides
ATE140929T1 (de) * 1990-11-08 1996-08-15 Japan Energy Corp Hirudinmutante, deren herstellung, antikoagulans, sekretorischer vektor, durch besagten vektor transformierter mikroorganismus und herstellung eines produkts durch besagten mikroorganismus
DE69233022T2 (de) 1991-11-04 2004-02-12 Genetics Institute, LLC, Cambridge Rekombinante knochenmorphogenetische protein heterodimere, zusammensetzungen und verfahren zur verwendung
DE69433530T2 (de) 1993-05-12 2005-01-05 Genetics Institute, LLC, Cambridge Bmp-11 zusammensetzungen
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
ATE319823T1 (de) 1993-12-07 2006-03-15 Inst Genetics Llc Bmp-12, bmp-13 und diese enthaltende sehne- induzierende zusammensetzungen
US6027919A (en) 1993-12-07 2000-02-22 Genetics Institute, Inc. BMP-12 and BMP-13 proteins and DNA encoding them
US5747662A (en) * 1995-03-01 1998-05-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US6242177B1 (en) 1995-03-01 2001-06-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US6331431B1 (en) * 1995-11-28 2001-12-18 Ixsys, Inc. Vacuum device and method for isolating periplasmic fraction from cells
WO1998039143A1 (fr) 1997-03-07 1998-09-11 Nagai Works Co., Ltd. Agrafeuse
RU2218407C2 (ru) 1997-04-16 2003-12-10 Юниджен Лэбораториз Инк. Прямая экспрессия пептидов в культуральные среды
SE9702401D0 (sv) 1997-06-19 1997-06-19 Astra Ab Pharmaceutical use
US6165748A (en) 1997-07-11 2000-12-26 Genetics Institute, Inc. Frazzled nucleotide sequences and expression products
US6506595B2 (en) * 1998-03-31 2003-01-14 Itoham Foods Inc. DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs
KR100316347B1 (ko) * 1998-09-15 2002-08-27 한미약품(주) 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법
KR100360594B1 (ko) 2000-01-19 2002-11-13 한미약품공업 주식회사 인간 인터페론 알파의 발현 분비벡터 및 이를 이용한인터페론 알파의 생산 방법
US6689599B1 (en) 2000-10-20 2004-02-10 Genetics Institute, Llc Aggrecanase molecules
US7083945B1 (en) 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
US7125701B2 (en) 2001-07-27 2006-10-24 Wyeth Aggrecanase molecules
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
AU2003207795B2 (en) 2002-01-31 2009-01-08 Wyeth Aggrecanase molecules
US7150983B2 (en) 2002-02-05 2006-12-19 Wyeth Truncated aggrecanase molecules
US7611866B2 (en) 2002-07-15 2009-11-03 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
BR0313083A (pt) 2002-07-29 2007-07-17 Wyeth Corp moléculas de adamts4 modificadas,polinucleotìdeo, vetor, processos para produção, composição farmacêutica, bem como uso das mesmas
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
ES2391457T3 (es) * 2003-08-13 2012-11-26 Sandoz Ag Procedimiento para la purificación de polipéptidos recombinantes
GB0319601D0 (en) * 2003-08-20 2003-09-24 Sandoz Ag Production process
BRPI0413720A (pt) 2003-09-12 2006-10-17 Wyeth Corp bastões e pastas sólidas de fosfato de cálcio injetável para liberação de proteìnas osteogênicas
WO2005089093A2 (en) 2003-11-21 2005-09-29 Dow Global Technologies Inc. Improved expression systems with sec-system secretion
US8227241B2 (en) 2004-03-12 2012-07-24 Unigene Laboratories, Inc. Bacterial host cell for the direct expression of peptides
JP2008507294A (ja) 2004-07-26 2008-03-13 ダウ グローバル テクノロジーズ インコーポレイティド 菌株遺伝子操作による改善されたタンパク質発現のための方法
ZA200704934B (en) * 2004-12-22 2010-09-29 Genentech Inc Methods for producing soluble multi-membrane-spanning proteins
JP4899436B2 (ja) * 2005-11-18 2012-03-21 マックス株式会社 ステープラ
CN101641441A (zh) 2007-01-31 2010-02-03 陶氏环球技术公司 用于提高表达的细菌前导序列
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
KR101530395B1 (ko) 2007-04-27 2015-06-19 피페넥스 인크. 향상된 수율 및(또는) 품질로 이종 단백질을 발현하는 특정 균주를 동정하기 위해 미생물 숙주를 신속히 스크리닝하는 방법
GB201106225D0 (en) * 2011-04-13 2011-05-25 Glaxosmithkline Biolog Sa Fermentation process
PL2968470T3 (pl) 2013-03-12 2021-05-31 The General Hospital Corporation Zmodyfikowane białka stanowiące substancję hamującą rozwój przewodów müllera (mis) i ich zastosowania do leczenia chorób
EP3079712B1 (de) 2013-12-11 2022-02-02 The General Hospital Corporation Verwendung von anti-müller-hormon (amh)-proteine zur kontrazeption und ovariellen reservekonservierung
EP3423588A1 (de) 2016-03-02 2019-01-09 Lonza Ltd Verbesserter fermentationsprozess

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
GR76959B (de) * 1981-01-02 1984-09-04 Univ New York State Res Found
JPS5869897A (ja) * 1981-10-20 1983-04-26 Gakuzo Tamura Ｄｎａ遺伝子
SE8204811L (sv) * 1982-08-23 1984-02-24 Sven Lofdahl Dna-fragment innefattande en signalsekvens
ZA837173B (en) * 1982-11-08 1984-05-30 Univ Case Western Reserve Genomic bovine growth hormone
AU2092983A (en) * 1982-11-08 1984-05-17 Genentech Inc. Porcine growth hormone produced by recombinant dna technology
JPS59135892A (ja) * 1983-01-25 1984-08-04 Kunio Yamane シグナルアミノ酸配列をコードするｄｎａ
US4711844A (en) * 1983-03-09 1987-12-08 Cetus Corporation Modified signal peptides

Also Published As

Publication number Publication date
EP0177343B1 (de) 1992-07-22
JPH06296491A (ja) 1994-10-25
DE3586386T2 (de) 1993-01-14
JPS6192575A (ja) 1986-05-10
JPH0815440B2 (ja) 1996-02-21
EP0177343A1 (de) 1986-04-09
DE3586386D1 (de) 1992-08-27
JP2521413B2 (ja) 1996-08-07

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EELA Cancelled due to lapse of time