CA2626525C - Conditionally immortalized long-term stem cells and methods of making and using such cells - Google Patents

Conditionally immortalized long-term stem cells and methods of making and using such cells Download PDF

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Publication number
CA2626525C
CA2626525C CA2626525A CA2626525A CA2626525C CA 2626525 C CA2626525 C CA 2626525C CA 2626525 A CA2626525 A CA 2626525A CA 2626525 A CA2626525 A CA 2626525A CA 2626525 C CA2626525 C CA 2626525C
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stem cell
cells
cell
protooncogene
protein
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English (en)
French (fr)
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CA2626525A1 (en
Inventor
John Cambier
Yosef Refaeli
Sara Ann Johnson
Brian Curtis Turner
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University of Colorado Boulder
National Jewish Health
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University of Colorado Boulder
National Jewish Health
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0636—T lymphocytes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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    • C12N15/09—Recombinant DNA-technology
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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CA2626525A 2005-10-18 2006-10-18 Conditionally immortalized long-term stem cells and methods of making and using such cells Active CA2626525C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3065947A CA3065947C (en) 2005-10-18 2006-10-18 Conditionally immortalized long-term stem cells and methods of making and using such cells

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US72813105P 2005-10-18 2005-10-18
US60/728,131 2005-10-18
US76599306P 2006-02-06 2006-02-06
US60/765,993 2006-02-06
PCT/US2006/040379 WO2007047583A2 (en) 2005-10-18 2006-10-18 Conditionally immortalized long-term stem cells and methods of making and using such cells

Related Child Applications (1)

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CA3065947A Division CA3065947C (en) 2005-10-18 2006-10-18 Conditionally immortalized long-term stem cells and methods of making and using such cells

Publications (2)

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CA2626525A1 CA2626525A1 (en) 2007-04-26
CA2626525C true CA2626525C (en) 2020-03-10

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CA2626525A Active CA2626525C (en) 2005-10-18 2006-10-18 Conditionally immortalized long-term stem cells and methods of making and using such cells
CA3065947A Active CA3065947C (en) 2005-10-18 2006-10-18 Conditionally immortalized long-term stem cells and methods of making and using such cells

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US (7) US8883507B2 (OSRAM)
EP (1) EP1942739B1 (OSRAM)
JP (8) JP2009511081A (OSRAM)
KR (2) KR101441693B1 (OSRAM)
CN (2) CN105483088B (OSRAM)
AT (1) ATE491022T1 (OSRAM)
AU (1) AU2006304392B2 (OSRAM)
CA (2) CA2626525C (OSRAM)
DE (1) DE602006018768D1 (OSRAM)
IL (7) IL286053B2 (OSRAM)
IN (1) IN2014DN06624A (OSRAM)
WO (1) WO2007047583A2 (OSRAM)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105483088B (zh) * 2005-10-18 2021-05-28 国家犹太健康中心 条件无限增殖化长期干细胞和制备和使用所述细胞的方法
DK2035549T3 (da) 2006-05-31 2014-10-13 Childrens Medical Center Abcb5-positive mesenkymale stamceller som immunmodulatorer
GB0619500D0 (en) * 2006-10-03 2006-11-08 Univ Keele Treatment of fibrosis
US8551968B2 (en) 2007-03-13 2013-10-08 National Jewish Health Methods for generation of antibodies
CA2723114C (en) * 2008-05-16 2018-02-27 Taiga Biotechnologies, Inc. Antibodies and processes for preparing the same
US9169462B2 (en) * 2008-07-21 2015-10-27 Taiga Biotechnologies, Inc. Methods for preparing mature erythrocytes from conditionally immortalized hematopoietic stem cells
ES2561599T3 (es) * 2008-08-28 2016-02-29 Taiga Biotechnologies, Inc. Moduladores de MYC, métodos de uso de los mismos, y métodos para identificar agentes que modulan MYC
US11542328B2 (en) 2008-11-14 2023-01-03 The Brigham And Women's Hospital, Inc. Therapeutic and diagnostic methods relating to cancer stem cells
EP3734281A3 (en) 2008-11-14 2021-01-27 The Brigham and Women's Hospital, Inc. Therapeutic and diagnostic methods relating to cancer stem cells
SG176117A1 (en) * 2009-06-29 2011-12-29 Ilya B Leskov Non-human mammal model of human hematopoietic cancer
IN2012DN02642A (OSRAM) * 2009-09-15 2015-09-11 Univ Tokyo
AU2014240253B2 (en) * 2009-09-15 2017-08-03 The University Of Tokyo Novel Method for Producing Differentiated Cells
US20120321595A1 (en) 2009-11-09 2012-12-20 The Brigham And Women's Hospital, Inc. Treatment of heart disease
US20130052169A1 (en) * 2010-05-06 2013-02-28 Stem Cell Medicine Ltd. Stem cell bank for personalized medicine
JP5861191B2 (ja) * 2010-09-30 2016-02-16 国立大学法人 熊本大学 ミエロイド系血液細胞の製造方法
US8889630B2 (en) 2011-12-23 2014-11-18 Carlos Lopez Method for hair regrowth using Granulocyte-Colony Stimulating Factor
US20130171115A1 (en) * 2011-12-30 2013-07-04 Gang Li Cell-mediated gene therapy for cancer using mesenchymal stem cells expressing a suicide gene
CN114645015A (zh) * 2012-07-20 2022-06-21 泰加生物工艺学公司 造血区室的增强的重建和自动重建
DK2964238T3 (da) 2013-02-19 2021-03-22 Childrens Medical Center Abcb5(+) stem cells for treating ocular disease
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
US9365825B2 (en) * 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
KR102319899B1 (ko) 2013-05-10 2021-11-01 칠드런'즈 메디컬 센터 코포레이션 상처 치유 및 조직 공학
KR101638403B1 (ko) * 2013-07-17 2016-07-11 아주대학교산학협력단 RGMc를 유효성분으로 포함하는 줄기세포 유도용 배지 조성물 및 이를 이용한 줄기세포의 유도방법
WO2015020993A2 (en) * 2013-08-05 2015-02-12 The Trustees Of The University Of Pennsylvania RNAi COMPOSITIONS AND METHODS FOR TREATMENT OF FRIEDREICH'S ATAXIA
CN103509101B (zh) * 2013-08-12 2016-11-02 中国科学院广州生物医药与健康研究院 一种扩增脐带血造血干细胞的细胞因子及其培养基
US20180051253A1 (en) 2015-03-18 2018-02-22 The Regents Of The University Of California Methods of preventing and reversing stem cell aging
CN106148266A (zh) * 2015-04-20 2016-11-23 烟台赛泽生物技术有限公司 一种免疫细胞用培养基及该培养基的添加剂
CN104928247B (zh) * 2015-07-02 2018-03-23 广州赛莱拉干细胞科技股份有限公司 一种神经干细胞培养基及神经干细胞贴壁培养方法
RU2018106515A (ru) 2015-07-21 2019-08-21 Зе Чилдрен'С Медикал Сентер Корпорейшн Pd-l1-экспрессирующие гемопоэтические стволовые клетки и применения
EP3347026A4 (en) 2015-09-09 2019-05-08 Seattle Children's Hospital (DBA Seattle Children's Research Institute) GENEMANIPULATION OF MACROPHAGES FOR IMMUNOTHERAPY
US11534466B2 (en) 2016-03-09 2022-12-27 Aal Scientifics, Inc. Pancreatic stem cells and uses thereof
CN105695511B (zh) * 2016-04-13 2025-01-21 中国人民解放军第三军医大学 一种永生化毛囊干细胞系及其制备方法
CN105713880A (zh) * 2016-04-20 2016-06-29 广东艾时代生物科技有限责任公司 一种造血干细胞体外扩增培养的无血清培养基及其应用
CN106279403B (zh) * 2016-08-16 2019-06-11 长春市海兰深生物医学技术有限公司 一种检测天然肺癌相关抗体的组合物、试剂盒和方法
JP6799683B2 (ja) 2016-12-02 2020-12-16 タイガ バイオテクノロジーズ，インク． ナノ粒子製剤
CN106754728B (zh) * 2016-12-30 2020-05-15 中国人民解放军总医院 后肾间充质细胞系及其制备方法与应用
FR3068366B1 (fr) * 2017-06-30 2023-11-24 Francais Du Sang Ets Procede de production de progeniteurs erythroides
CA3071448A1 (en) * 2017-08-03 2019-02-07 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of cancer
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation TREATMENT OF TYPE 1 DIABETES AND AUTOIMMUNE DISEASES OR DISORDERS
US12023357B2 (en) 2017-10-13 2024-07-02 Hackensack University Medical Center Rejuvenated aged hematopoietic stem cells and methods of use
CN107937342B (zh) * 2017-11-06 2021-07-06 中国人民解放军第四军医大学 一种通过内皮细胞来源的外泌体扩增神经干细胞的方法
CN108070567A (zh) * 2018-01-19 2018-05-25 皓昇莱生物制药有限公司 一种永生化的尿液来源细胞株及其构建方法
WO2019210109A1 (en) 2018-04-25 2019-10-31 Children's Medical Center Corporation Abcb5 ligands and substrates
SG11202103096PA (en) * 2018-10-12 2021-04-29 Reneuron Ltd Induced pluripotent cell comprising a controllable transgene for conditional immortalisation
GB201902068D0 (en) * 2019-02-14 2019-04-03 Cambridge Entpr Ltd Methods of reactivating proliferative capacity non-proliferative tissues
AU2020272664A1 (en) 2019-04-08 2021-11-04 Taiga Biotechnologies, Inc. Compositions and methods for the cry opreservation of immune cells
US12370217B2 (en) 2019-05-14 2025-07-29 Htyr Acquisition Llc Compositions and methods for treating T cell exhaustion
US20220282220A1 (en) * 2019-07-24 2022-09-08 Sl Bigen Inc. Method for preparation of immortalized stem cell line and use thereof
CN110622921B (zh) * 2019-09-29 2021-06-15 江苏大学 阿尔茨海默病病变区FoxG1过表达小鼠模型的构建方法及应用
GB202005494D0 (en) * 2020-04-15 2020-05-27 Reneuron Ltd Induced pluripotent cell comprising a contollable transgene for conditional immortalisation
KR20220057859A (ko) 2020-10-30 2022-05-09 전상수 펜터리
WO2022097716A1 (ja) 2020-11-06 2022-05-12 株式会社Trans Chromosomics 可逆的不死化細胞の製造方法
CN113444730A (zh) * 2021-03-17 2021-09-28 昆明市延安医院 一种原发性肝细胞klotho基因转导干细胞筛选构建方法
EP4365283A4 (en) * 2021-06-30 2025-06-11 National University Corporation Chiba University Methods for improving the proliferative properties of common myeloid progenitor cells (CMP) or myelocytic progenitor cells
CN113583965A (zh) * 2021-08-05 2021-11-02 大连干细胞与精准医学创新研究院 一种条件永生化人神经干细胞来源细胞膜纳米囊泡制剂及其制备方法和应用
JP2024539140A (ja) * 2021-10-20 2024-10-28 ユニヴァーシティ オヴ ロチェスター グリア前駆細胞を若返らせるための方法及び若返ったグリア前駆細胞それ自体
JP7773257B2 (ja) 2022-03-28 2025-11-19 合同会社HiRoko Science ｍｉＲ－１４０を含む医薬組成物及びその製造方法

Family Cites Families (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
JPS62501537A (ja) * 1984-12-21 1987-06-25 テクニクロン・リサ−チ・パ−トナ−ズ・ワン リンパ球細胞を電気的に不滅化するための方法
DE3531430A1 (de) 1985-09-03 1987-03-05 Biotest Pharma Gmbh Verfahren zur gewinnung von komponenten aus einer fluessigkeit mit in raeumlich getrennten bereichen vorliegenden komponenten
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US4900322A (en) 1986-09-22 1990-02-13 Adams James D Blood component pooling valve and kit
US5476996A (en) * 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5399493A (en) 1989-06-15 1995-03-21 The Regents Of The University Of Michigan Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
US5674980A (en) * 1989-12-21 1997-10-07 Biogen Inc Fusion protein comprising tat-derived transport moiety
US5804604A (en) 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5849288A (en) * 1990-01-15 1998-12-15 Yeda Research And Development Co. Ltd. Method for production of monoclonal antibodies in chimeric mice or rats having xenogeneic antibody-producing cells
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
US5843728A (en) 1991-03-07 1998-12-01 The General Hospital Corporation Redirection of cellular immunity by receptor chimeras
US6753162B1 (en) 1991-03-07 2004-06-22 The General Hospital Corporation Targeted cytolysis of HIV-infected cells by chimeric CD4 receptor-bearing cells
IL101147A (en) 1991-03-07 2004-06-20 Gen Hospital Corp Redirection of cellular immunity by receptor chimeras
US5912170A (en) 1991-03-07 1999-06-15 The General Hospital Corporation Redirection of cellular immunity by protein-tyrosine kinase chimeras
US6004811A (en) 1991-03-07 1999-12-21 The Massachussetts General Hospital Redirection of cellular immunity by protein tyrosine kinase chimeras
US5851828A (en) 1991-03-07 1998-12-22 The General Hospital Corporation Targeted cytolysis of HIV-infected cells by chimeric CD4 receptor-bearing cells
AU781922B2 (en) 1991-12-17 2005-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5289858A (en) 1991-12-18 1994-03-01 Abbott Laboratories System for accommodating withdrawal of liquid from a bulk supply
US8211422B2 (en) 1992-03-18 2012-07-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric receptor genes and cells transformed therewith
ATE173016T1 (de) 1992-08-21 1998-11-15 Biogen Inc Von tat abgeleitete transportpolypeptide
US5580760A (en) 1993-02-22 1996-12-03 The United States Of America As Represented By The Department Of Health And Human Services FUSE binding protein and cDNA therefor
US5824837A (en) * 1993-07-22 1998-10-20 Merck & Co., Inc. Expression of human interleukin-1β in a transgenic animal
US5599705A (en) * 1993-11-16 1997-02-04 Cameron; Robert B. In vitro method for producing differentiated universally compatible mature human blood cells
JPH09510088A (ja) 1994-03-03 1997-10-14 アレクション・ファーマシューティカル・インク 最終補体インヒビター融合遺伝子およびタンパク質
US5827642A (en) 1994-08-31 1998-10-27 Fred Hutchinson Cancer Research Center Rapid expansion method ("REM") for in vitro propagation of T lymphocytes
US6713247B1 (en) * 1996-09-03 2004-03-30 Signal Pharmaceuticials, Inc. Human CNS cell lines and methods of use therefor
AU734827B2 (en) 1997-05-21 2001-06-21 Board Of Trustees Of The Leland Stanford Junior University Composition and method for enhancing transport across biological membranes
EP0893493A3 (de) * 1997-07-21 2002-12-04 Aventis Pharma Deutschland GmbH Genetisch veränderte Zellen und deren Verwendung in der Prophylaxe oder Therapie von Erkrankungen
GB9720585D0 (en) 1997-09-26 1997-11-26 Smithkline Beecham Biolog Vaccine
AU1818299A (en) 1997-12-10 1999-06-28 Washington University Anti-pathogen system and methods of use thereof
US6861257B1 (en) 1998-04-08 2005-03-01 Shionogi & Co., Ltd. Methods for isolation of osteoclast precursor cells and inducing their differentiation into osteoclasts
US6835567B1 (en) 1998-04-14 2004-12-28 Signal Pharmaceuticals, Inc. PNS cell lines and methods of use therefor
US6451558B1 (en) * 1998-08-03 2002-09-17 Novartis Ag Genes in the control of hematopoiesis
US6913925B1 (en) * 1998-08-12 2005-07-05 Signal Pharmaceuticals Llc Human mesencephalon cell lines and methods of use therefor
AU7497000A (en) 1999-02-28 2000-11-14 Washington University Novel transduction molecules and methods for using same
AU4070500A (en) * 1999-04-05 2000-10-23 Biocrystal Limited Assay kits and methods for immune complex-mediated activation involving shed antigens
ATE342275T1 (de) 1999-04-12 2006-11-15 Heart Biosystems Gmbh Vorübergehend-immortalisierte zellen zur verwendung in gentherapie
US6358739B1 (en) 1999-04-12 2002-03-19 Modex Therapeutiques, S.A. Transiently immortalized cells
US6451601B1 (en) * 1999-04-12 2002-09-17 Modex Therapeutiques, S.A. Transiently immortalized cells for use in gene therapy
US7135287B1 (en) * 1999-10-02 2006-11-14 Biosite, Inc. Human antibodies
US7311920B1 (en) * 1999-10-08 2007-12-25 University Of Maryland Biotechnology Institute Virus coat protein/receptor chimeras and methods of use
EP1228233B1 (en) * 1999-11-10 2007-01-10 Rigel Pharmaceuticals, Inc. Methods and compositions comprising renilla gfp
EP1103615A1 (en) 1999-11-25 2001-05-30 Universite De Geneve Vectors capable of immortalizing non-dividing cells and cells immortalized with said vectors
US20010049393A1 (en) * 1999-12-07 2001-12-06 Whitehead Institute For Biomedical Research Methods for defining MYC target genes and uses thereof
EP1285577A4 (en) * 2000-05-15 2007-05-09 Sonoko Habu CHIMERIC MOUSE HAVING IMMUNITY CREATED BY USE OF HUMAN CD34 POSITIVE CELLS AND USE THEREOF
US20020155127A1 (en) 2000-06-02 2002-10-24 Danher Wang Genetic vaccine against human immunodeficiency virus
US20030072794A1 (en) * 2000-06-09 2003-04-17 Teni Boulikas Encapsulation of plasmid DNA (lipogenes™) and therapeutic agents with nuclear localization signal/fusogenic peptide conjugates into targeted liposome complexes
US20070248618A1 (en) * 2004-03-16 2007-10-25 Cohen David I Tat-Based vaccine Compositions and Methods of Making and Using Same
EP1326969B1 (en) 2000-09-25 2009-04-15 Genetronics, Inc. Improved system for regulation of transgene expression
WO2002036738A2 (en) 2000-10-30 2002-05-10 Kalobios, Inc. Affinity maturation by competitive selection
US20040241140A1 (en) 2000-11-01 2004-12-02 Pavlakis George N. Expression vectors able to elicit improved immune response and methods of using same
JP3753321B2 (ja) * 2000-12-01 2006-03-08 財団法人実験動物中央研究所 異種細胞の生着、分化および増殖に適したマウスの作出方法、該方法により作出されたマウスならびにそのマウスの用途
WO2002057436A2 (en) 2001-01-19 2002-07-25 Gendel Limited Red blood cell from a transgenic animal as vehicle for polypeptide delivery
JP3454818B1 (ja) * 2001-03-16 2003-10-06 直哉 小林 肝臓細胞の増殖方法、該方法により得られる肝臓細胞、およびその用途
DE60228082D1 (de) * 2001-05-31 2008-09-18 Novartis Ag Nten und neue liganden und pharmazeutische zusammensetzungen
US7329731B2 (en) 2001-08-31 2008-02-12 Medigene Limited Soluble T cell receptor
CN1408850A (zh) * 2001-09-18 2003-04-09 王虹 人血树突状细胞永生化技术
CN100506978C (zh) 2001-09-21 2009-07-01 中国人民解放军军事医学科学院野战输血研究所 一种新的造血干/祖细胞的富集方法及其体外定向诱导分化
GB0124391D0 (en) 2001-10-11 2001-11-28 Gene Expression Technologies L Control of gene expression
US20030147887A1 (en) 2001-11-02 2003-08-07 Shen-Wu Wang B-cell lymphoma specific antigen for use in diagnosis and treatment of B-cell malignancies
EP1321034A1 (en) * 2001-12-21 2003-06-25 Pfizer Products Inc. Disruption of the phosphodieterase 10 gene
US7745140B2 (en) 2002-01-03 2010-06-29 The Trustees Of The University Of Pennsylvania Activation and expansion of T-cells using an engineered multivalent signaling platform as a research tool
AU2003202908A1 (en) 2002-01-03 2003-07-24 The Trustees Of The University Of Pennsylvania Activation and expansion of t-cells using an engineered multivalent signaling platform
AU2003216288B2 (en) 2002-02-13 2009-09-24 Duke University Modulation of immune response by non-peptide binding stress response polypeptides
CN1240439C (zh) 2002-03-28 2006-02-08 南京凯基生物科技发展有限公司 肿瘤基因开关药物
US7211191B2 (en) 2004-09-30 2007-05-01 Thermogenesis Corp. Blood component separation method and apparatus
EP1499180A4 (en) 2002-04-16 2006-09-13 Dana Farber Cancer Inst Inc CANCER MODELS
GB2387599B (en) 2002-04-17 2005-08-10 Jason Peter Brown Methods for producing antibodies
AU2003228962A1 (en) 2002-05-10 2003-11-11 New Century Pharmaceuticals, Inc. Ferritin fusion proteins for use in vaccines and other applications
JP2006502704A (ja) * 2002-05-16 2006-01-26 バヴァリアン・ノルディック・アクティーゼルスカブ Ｈｉｖ調節／アクセサリータンパク質の融合タンパク質
AU2003237259B2 (en) * 2002-05-29 2008-01-24 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
DK1545204T3 (en) 2002-09-06 2016-11-14 The Government Of The Us Secretary Dept Of Health And Human Services Immunotherapy with in vitro selected antigen-specific lymphocytes following non-myeloablative lymphodepletive chemotherapy
CA2501870C (en) 2002-10-09 2013-07-02 Avidex Limited Single chain recombinant t cell receptors
EP1408114B1 (de) * 2002-10-11 2007-01-03 Imvision GmbH Moduläre Antigen-Transporter Moleküle (MAT-Moleküle) zur Modulierung von Immunreaktionen, zugehörige Konstrukte, Verfahren und Verwendungen
AU2003274633A1 (en) 2002-10-15 2004-05-04 Ramot At Tel-Aviv University Ltd. Erythrocyte differentiation factor, gene encoding same, and methods of use thereof
AU2003276403B2 (en) 2002-11-09 2010-04-15 Adaptimmune Limited T cell receptor display
EP1570061A2 (en) 2002-12-05 2005-09-07 Imperial College Innovations Limited Control of apoptosis using a complex of an oligonucleotide and a regulatory peptide
GB0304068D0 (en) 2003-02-22 2003-03-26 Avidex Ltd Substances
WO2004084805A2 (en) 2003-03-19 2004-10-07 The J. David Gladstone Institutes Acetylated tat polypeptides and methods of use thereof
JP2006524991A (ja) 2003-05-08 2006-11-09 エクサイト セラピーズ インコーポレーティッド 抗原特異的ｔ細胞の作製および単離の方法
WO2005014785A2 (en) 2003-06-18 2005-02-17 The George Washington University Conditionally-immortalized hematopoietic progenitor cell lines
AU2004316996A1 (en) 2003-06-20 2005-09-15 The Regents Of The University Of California Polypeptide transduction and fusogenic peptides
IL161903A0 (en) 2003-07-17 2005-11-20 Gamida Cell Ltd Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs
US7705049B2 (en) * 2004-01-21 2010-04-27 New York University Methods for treating non-melanoma cancers with PABA
JP2005232148A (ja) * 2004-02-03 2005-09-02 Technion Research & Development Foundation Ltd 神経保護剤としてプロパルギルアミンの使用
NZ550810A (en) 2004-05-19 2009-05-31 Immunocore Ltd High affinity NY-ESO T cell receptor
AU2005246073B2 (en) 2004-05-19 2010-10-28 Adaptimmune Limited Method of improving T cell receptors
ATE475669T1 (de) 2004-06-29 2010-08-15 Immunocore Ltd Einen modifizierten t-zellen-rezeptor exprimierende zellen
US20060068469A1 (en) 2004-08-17 2006-03-30 Research Development Foundation Bacterial vector systems
GB0420963D0 (en) 2004-09-21 2004-10-20 Reneuron Ltd Hepatocyte
US7767453B2 (en) * 2004-10-20 2010-08-03 Whitehead Institute For Biomedical Research Cultured hematopoietic stem cells and method for expansion and analysis thereof
US8795650B2 (en) 2004-12-09 2014-08-05 The Regents Of The University Of California Derivation of unlimited quantities of neutrophils or monocyte/dendritic cells
US20060156422A1 (en) * 2005-01-10 2006-07-13 Medical Research Council Methods and compositions for the generation of antibodies
WO2006104978A2 (en) 2005-03-25 2006-10-05 Curagen Corporation Antibodies against the tenascin major antigens
CA2609342A1 (en) 2005-04-26 2006-11-02 Andrei L. Gartel Nucleoside compounds and methods of use thereof
WO2006125962A2 (en) 2005-05-25 2006-11-30 Medigene Limited T cell receptors which specifically bind to vygfvracl-hla-a24
AU2006282799B2 (en) * 2005-08-26 2012-11-29 Healthpartners Research & Education Methods for treatment of headaches by administration of oxytocin
US20070047583A1 (en) 2005-08-29 2007-03-01 Siemens Aktiengesellschaft Method for using a short address in a packet header
CN105483088B (zh) 2005-10-18 2021-05-28 国家犹太健康中心 条件无限增殖化长期干细胞和制备和使用所述细胞的方法
CA2626584A1 (en) * 2005-11-04 2007-05-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of nav1.8 gene
EP1792627A1 (en) 2005-12-05 2007-06-06 ImVisioN AG Modulation of the immune response by administration of intralymphatic transduction allergen (ITAG-)-molecules
US8088379B2 (en) 2006-09-26 2012-01-03 The United States Of America As Represented By The Department Of Health And Human Services Modified T cell receptors and related materials and methods
EP2087000A2 (en) 2006-09-29 2009-08-12 Immunocore Ltd. T cell therapies
US8551968B2 (en) 2007-03-13 2013-10-08 National Jewish Health Methods for generation of antibodies
US20110218210A1 (en) 2007-11-02 2011-09-08 Taiga Biotechnologies Compounds for treating abnormal cellular proliferation
WO2009117439A2 (en) 2008-03-17 2009-09-24 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
CA2723114C (en) 2008-05-16 2018-02-27 Taiga Biotechnologies, Inc. Antibodies and processes for preparing the same
EP2304035A2 (en) 2008-06-13 2011-04-06 Life & Brain GmbH Fusion protein and use thereof
US9169462B2 (en) * 2008-07-21 2015-10-27 Taiga Biotechnologies, Inc. Methods for preparing mature erythrocytes from conditionally immortalized hematopoietic stem cells
ES2561599T3 (es) 2008-08-28 2016-02-29 Taiga Biotechnologies, Inc. Moduladores de MYC, métodos de uso de los mismos, y métodos para identificar agentes que modulan MYC
PT3031907T (pt) 2008-11-06 2021-01-28 Univ Indiana Res & Tech Corp Materiais e métodos para melhorar procedimentos de enxerto de células estaminais hematopoiéticas
CN102272153B (zh) 2008-11-24 2015-04-15 德国慕尼黑亥姆霍兹研究中心健康和环境有限公司 高亲和力t细胞受体及其应用
WO2011100477A2 (en) 2010-02-10 2011-08-18 Taiga Biotechnologies, Inc. Antibodies and processes for preparing the same
US20120107317A1 (en) 2010-10-27 2012-05-03 The University Of Hong Kong Use of cytoplasmic c-myc for regulating immune responses
US20120244133A1 (en) 2011-03-22 2012-09-27 The United States of America, as represented by the Secretary, Department of Health and Methods of growing tumor infiltrating lymphocytes in gas-permeable containers
EP3766896B1 (en) 2011-09-15 2025-11-05 The United States of America, as represented by The Secretary, Department of Health and Human Services T cell receptors recognizing hla-a1- or hla-cw7-restricted mage
CN104395462B (zh) 2012-05-03 2017-09-26 弗雷德哈钦森癌症研究中心 增强亲和力的t细胞受体及其制备方法
DE102012209673A1 (de) 2012-06-08 2013-12-24 Artcline Gmbh Verfahren zum Herstellen einer Leukozytenpräparation und Leukozytenpräparation
CN114645015A (zh) 2012-07-20 2022-06-21 泰加生物工艺学公司 造血区室的增强的重建和自动重建
WO2014018863A1 (en) 2012-07-27 2014-01-30 The Board Of Trustees Of The University Of Illinois Engineering t-cell receptors
US20140109246A1 (en) 2012-09-24 2014-04-17 The Regents Of The University Of Colorado, A Body Corporate Methods of generating xenochimaeric mice with tumor and hematopoietic system from the same heterologous species
GB2508414A (en) 2012-11-30 2014-06-04 Max Delbrueck Centrum Tumour specific T cell receptors (TCRs)
US20160010058A1 (en) 2013-03-01 2016-01-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Serv Methods of producing enriched populations of tumor-reactive t cells from tumor
CA2902448C (en) 2013-03-01 2023-04-18 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of producing enriched populations of tumor reactive t cells from peripheral blood
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
US9365825B2 (en) 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
KR20200138445A (ko) 2014-04-24 2020-12-09 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 입양 세포 요법 생성물을 생성하기 위한 유도 만능 줄기 세포의 응용
KR102825102B1 (ko) 2014-12-24 2025-06-26 넥스이뮨, 인크. 면역요법을 위한 나노입자 조성물 및 방법
CN109952313B (zh) 2016-01-15 2023-12-19 伯克利之光生命科技公司 生产患者特异性抗癌治疗剂的方法和其治疗方法
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma

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