CA2493539C - A method for purifying preproinsulin - Google Patents

A method for purifying preproinsulin Download PDF

Info

Publication number
CA2493539C
CA2493539C CA2493539A CA2493539A CA2493539C CA 2493539 C CA2493539 C CA 2493539C CA 2493539 A CA2493539 A CA 2493539A CA 2493539 A CA2493539 A CA 2493539A CA 2493539 C CA2493539 C CA 2493539C
Authority
CA
Canada
Prior art keywords
gly
leu
glu
preproinsulin
ser
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2493539A
Other languages
English (en)
French (fr)
Other versions
CA2493539A1 (en
Inventor
Horst Thurow
Hans Blumenstock
Chantalle Havenith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis Deutschland GmbH
Original Assignee
Sanofi Aventis Deutschland GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Aventis Deutschland GmbH filed Critical Sanofi Aventis Deutschland GmbH
Publication of CA2493539A1 publication Critical patent/CA2493539A1/en
Application granted granted Critical
Publication of CA2493539C publication Critical patent/CA2493539C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA2493539A 2002-08-01 2003-07-18 A method for purifying preproinsulin Expired - Fee Related CA2493539C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10235168A DE10235168A1 (de) 2002-08-01 2002-08-01 Verfahren zur Reinigung von Preproinsulin
DE10235168.6 2002-08-01
PCT/EP2003/007820 WO2004013176A1 (de) 2002-08-01 2003-07-18 Verfahren zur reinigung von preproinsulin

Publications (2)

Publication Number Publication Date
CA2493539A1 CA2493539A1 (en) 2004-02-12
CA2493539C true CA2493539C (en) 2012-01-31

Family

ID=30128614

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2493539A Expired - Fee Related CA2493539C (en) 2002-08-01 2003-07-18 A method for purifying preproinsulin

Country Status (16)

Country Link
US (2) US20050080000A1 (https=)
EP (1) EP1527097B1 (https=)
JP (1) JP4519646B2 (https=)
AT (1) ATE384742T1 (https=)
AU (1) AU2003254375B2 (https=)
BR (1) BR0313131A (https=)
CA (1) CA2493539C (https=)
CY (1) CY1108046T1 (https=)
DE (2) DE10235168A1 (https=)
DK (1) DK1527097T3 (https=)
ES (1) ES2297223T3 (https=)
IL (1) IL166438A (https=)
MX (1) MXPA05001042A (https=)
PT (1) PT1527097E (https=)
SI (1) SI1527097T1 (https=)
WO (1) WO2004013176A1 (https=)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6428771B1 (en) * 1995-05-15 2002-08-06 Pharmaceutical Discovery Corporation Method for drug delivery to the pulmonary system
US9006175B2 (en) 1999-06-29 2015-04-14 Mannkind Corporation Potentiation of glucose elimination
ES2569916T3 (es) * 1999-06-29 2016-05-13 Mannkind Corporation Formulaciones farmacéuticas que comprenden insulina complejada con una dicetopiperazina
US6923175B2 (en) 2002-03-20 2005-08-02 Mannkind Corporation Inhalation apparatus
US7338171B2 (en) * 2003-10-27 2008-03-04 Jen-Chuen Hsieh Method and apparatus for visual drive control
ES2584867T3 (es) * 2004-01-12 2016-09-29 Mannkind Corporation Un método que reduce los niveles séricos de proinsulina en diabéticos de tipo 2
DE602005014962D1 (de) * 2004-03-12 2009-07-30 Biodel Inc Insulinzusammensetzungen mit verbesserter wirkstoffabsorption
US20080090753A1 (en) * 2004-03-12 2008-04-17 Biodel, Inc. Rapid Acting Injectable Insulin Compositions
ATE486064T1 (de) 2004-08-20 2010-11-15 Mannkind Corp Katalyse der diketopiperazinsynthese
KR20130066695A (ko) 2004-08-23 2013-06-20 맨카인드 코포레이션 약물 전달용 디케토피페라진염, 디케토모르포린염 또는 디케토디옥산염
MX373000B (es) 2005-09-14 2020-05-21 Mannkind Corp Metodo para formulacion de farmaco basado en el aumento de la afinidad de agentes activos hacia las superficies de microparticulas cristalinas.
US7713929B2 (en) 2006-04-12 2010-05-11 Biodel Inc. Rapid acting and long acting insulin combination formulations
WO2007041481A1 (en) * 2005-09-29 2007-04-12 Biodel, Inc. Rapid acting and prolonged acting insulin preparations
US8084420B2 (en) * 2005-09-29 2011-12-27 Biodel Inc. Rapid acting and long acting insulin combination formulations
EP1986679B1 (en) 2006-02-22 2017-10-25 MannKind Corporation A method for improving the pharmaceutic properties of microparticles comprising diketopiperazine and an active agent
WO2007121256A2 (en) * 2006-04-12 2007-10-25 Biodel, Inc. Rapid acting and long acting insulin combination formulations
US20090175840A1 (en) * 2008-01-04 2009-07-09 Biodel, Inc. Insulin formulations for insulin release as a function of tissue glucose levels
CN101827626B (zh) 2008-06-13 2015-03-18 曼金德公司 干粉吸入器和用于药物输送的系统
US8485180B2 (en) 2008-06-13 2013-07-16 Mannkind Corporation Dry powder drug delivery system
ES2421385T3 (es) 2008-06-20 2013-09-02 Mannkind Corp Aparato interactivo y procedimiento para establecer el perfil, en tiempo real, de esfuerzos de inhalación
TWI532497B (zh) 2008-08-11 2016-05-11 曼凱公司 超快起作用胰島素之用途
CN102256618A (zh) 2008-10-17 2011-11-23 赛诺菲-安万特德国有限公司 胰岛素和glp-1激动剂的组合
US8314106B2 (en) 2008-12-29 2012-11-20 Mannkind Corporation Substituted diketopiperazine analogs for use as drug delivery agents
US9060927B2 (en) * 2009-03-03 2015-06-23 Biodel Inc. Insulin formulations for rapid uptake
DK2405963T3 (da) 2009-03-11 2013-12-16 Mannkind Corp Apparat, system og fremgangsmåde til at måle modstand i en inhalator
KR102584844B1 (ko) 2009-06-12 2023-10-04 맨카인드 코포레이션 한정된 비표면적을 갖는 디케토피페라진 마이크로입자
PL2451437T3 (pl) 2009-07-06 2017-05-31 Sanofi-Aventis Deutschland Gmbh Wodne preparaty insuliny zawierające metioninę
EP2464655B1 (en) * 2009-08-11 2017-02-15 Biocon Limited Chromatographic processes
CA2778698A1 (en) 2009-11-03 2011-05-12 Mannkind Corporation An apparatus and method for simulating inhalation efforts
NZ599847A (en) 2009-11-13 2013-09-27 Sanofi Aventis Deutschland Pharmaceutical composition comprising a glp-1 agonist and methionine
TR201809460T4 (tr) 2009-11-13 2018-07-23 Sanofi Aventis Deutschland Bir GLP- 1-agonisti, bir insülin ve metiyonin içeren farmasötik bileşim.
AU2011202239C1 (en) 2010-05-19 2017-03-16 Sanofi Long-acting formulations of insulins
CN102985125A (zh) 2010-06-21 2013-03-20 曼金德公司 干粉药物输送系统和方法
WO2012028172A1 (en) 2010-08-30 2012-03-08 Sanofi-Aventis Deutschland Gmbh Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2
AU2012213432B2 (en) 2011-02-01 2016-10-13 Novo Nordisk A/S Purification of insulin
DE102011003944A1 (de) 2011-02-10 2012-08-16 Oxprotect Gmbh Detektion und Entfernung von missgefalteten Proteinen/Peptiden
SG194034A1 (en) 2011-04-01 2013-11-29 Mannkind Corp Blister package for pharmaceutical cartridges
CN102219851B (zh) 2011-05-09 2012-05-30 甘李药业有限公司 甘精胰岛素结晶的制备方法
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
WO2012174472A1 (en) 2011-06-17 2012-12-20 Mannkind Corporation High capacity diketopiperazine microparticles
AR087693A1 (es) 2011-08-29 2014-04-09 Sanofi Aventis Deutschland Combinacion farmaceutica para uso en el control glucemico en pacientes con diabetes de tipo 2
TWI559929B (en) 2011-09-01 2016-12-01 Sanofi Aventis Deutschland Pharmaceutical composition for use in the treatment of a neurodegenerative disease
MX2014004983A (es) 2011-10-24 2014-09-22 Mannkid Corp Metodos y composiciones para tratar dolor.
KR102264177B1 (ko) 2012-07-12 2021-06-11 맨카인드 코포레이션 건조 분말 약물 전달 시스템 및 방법
WO2014066856A1 (en) 2012-10-26 2014-05-01 Mannkind Corporation Inhalable influenza vaccine compositions and methods
EP2931301B2 (en) 2012-12-17 2021-09-15 Merck Sharp & Dohme Corp. Process for purifying insulin and analogues thereof
EP3587404B1 (en) 2013-03-15 2022-07-13 MannKind Corporation Microcrystalline diketopiperazine compositions, methods for preparation and use thereof
EP2983697B1 (en) 2013-04-03 2018-10-31 Sanofi Treatment of diabetes mellitus by long acting formulations of insulins
MX375448B (es) 2013-07-18 2025-03-06 Mannkind Corp Composiciones farmacéuticas en polvo seco estables al calor y métodos.
EP3030294B1 (en) 2013-08-05 2020-10-07 MannKind Corporation Insufflation apparatus
US10307464B2 (en) 2014-03-28 2019-06-04 Mannkind Corporation Use of ultrarapid acting insulin
CN106794156B (zh) * 2014-07-08 2021-03-09 美药星制药股份有限公司 微粒化胰岛素、微粒化胰岛素类似物及其制备方法
US10561806B2 (en) 2014-10-02 2020-02-18 Mannkind Corporation Mouthpiece cover for an inhaler
PE20171622A1 (es) 2014-12-12 2017-11-02 Sanofi Aventis Deutschland Formulacion de relacion fija de insulina glargina/lixisenatida
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
US10322168B2 (en) 2016-01-07 2019-06-18 Amphastar Pharmaceuticals, Inc. High-purity inhalable particles of insulin and insulin analogues, and high-efficiency methods of manufacturing the same
CN113773392B (zh) * 2020-06-09 2023-04-07 宁波鲲鹏生物科技有限公司 一种甘精胰岛素的制备方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4783441A (en) * 1979-04-30 1988-11-08 Hoechst Aktiengesellschaft Aqueous protein solutions stable to denaturation
IL84110A (en) 1986-10-14 1992-11-15 Lilly Co Eli Process for transforming a human insulin precursor to a human insulin
JPH0832726B2 (ja) * 1987-06-08 1996-03-29 武田薬品工業株式会社 ラットｂＦＧＦおよびその製造法
DE3726655A1 (de) * 1987-08-11 1989-02-23 Hoechst Ag Verfahren zur isolierung basischer proteine aus proteingemischen, welche solche basischen proteine enthalten
US5358857A (en) * 1989-08-29 1994-10-25 The General Hospital Corp. Method of preparing fusion proteins
IL95495A (en) * 1989-08-29 1996-10-16 Hoechst Ag Fusion proteins their preparation and use
JP2916206B2 (ja) * 1990-04-28 1999-07-05 帝國製薬株式会社 生理活性ペプチド
DK0600372T3 (da) * 1992-12-02 1997-08-11 Hoechst Ag Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer.
US5886154A (en) * 1997-06-20 1999-03-23 Lebing; Wytold R. Chromatographic method for high yield purification and viral inactivation of antibodies
DE19735711C2 (de) * 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
KR100253916B1 (ko) * 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
DE19947456A1 (de) * 1999-10-02 2001-04-05 Aventis Pharma Gmbh C-Peptid zur verbesserten Herstellung von Insulin und Insulinanaloga
DE10008064B4 (de) * 2000-02-22 2009-07-02 Siemens Ag Verfahren zum Anpassen der einem Turbo-Codierer zuzuführenden Datenblöcke und entsprechende Kommunikationsvorrichtung
ATE356138T1 (de) * 2000-02-22 2007-03-15 Applied Research Systems Gereinigtes recombinantes hlh mit spezifischer bioaktivität und verfahren zu dessen reinigung

Also Published As

Publication number Publication date
AU2003254375B2 (en) 2009-01-08
JP2006513978A (ja) 2006-04-27
US20060183666A1 (en) 2006-08-17
US20050080000A1 (en) 2005-04-14
SI1527097T1 (sl) 2008-04-30
AU2003254375A1 (en) 2004-02-23
ATE384742T1 (de) 2008-02-15
JP4519646B2 (ja) 2010-08-04
DE50309087D1 (https=) 2008-03-13
PT1527097E (pt) 2008-02-25
ES2297223T3 (es) 2008-05-01
BR0313131A (pt) 2005-07-05
DK1527097T3 (da) 2008-05-26
EP1527097A1 (de) 2005-05-04
CY1108046T1 (el) 2013-09-04
WO2004013176A1 (de) 2004-02-12
US7803763B2 (en) 2010-09-28
IL166438A (en) 2010-11-30
DE10235168A1 (de) 2004-02-12
MXPA05001042A (es) 2005-04-08
CA2493539A1 (en) 2004-02-12
EP1527097B1 (de) 2008-01-23
IL166438A0 (en) 2006-01-15

Similar Documents

Publication Publication Date Title
CA2493539C (en) A method for purifying preproinsulin
KR100574580B1 (ko) 정확하게 결합된 시스틴 브릿지를 갖는 인슐린 또는 인슐린유도체를 수득하는 방법
US20180194801A1 (en) Method for isolating and purifying recombinant human serum albumin from transgenic rice grain
CN113121705B (zh) 用于制备短肽混合物的融合蛋白、目的多肽、短肽混合物的制备方法及应用
JP4975895B2 (ja) 高圧液体クロマトグラフィーによるインシュリンの単離方法
RU2037500C1 (ru) Способ очистки инсулина и/или его производных
Markussen et al. Immobilized insulin for high capacity affinity chromatography of insulin receptors.
JPH07265092A (ja) 正しく連結されたシスチン架橋を有するインシュリンを得る方法
JPH06505010A (ja) 正確にフォールドされた完全なインスリン様成長因子−１の精製方法
TW201144325A (en) Selective enrichment of antibodies
Edmundson et al. Isolation and characterization of concanavalin A polypeptide chains
DE69120409T2 (de) Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte
US6906183B2 (en) Process for the separation of glycosylated and nonglycosylated proteins
EP3344651B1 (en) A process for obtaining insulin with correctly formed disulfide bonds
RU2081122C1 (ru) Способ выделения и очистки инсулина или его биотехнологических предшественников
KR20050054005A (ko) 인체 인터페론 알파의 제조방법
EP1567657A1 (en) Process for purifying a fermentation-derived product
CN113984911B (zh) 一种同时分析利拉鲁肽及其Boc-利拉鲁肽主链的色谱方法
RU2126690C1 (ru) Способ очистки инсулина-сырца, получаемого из поджелудочной железы свиней
KR100341297B1 (ko) 재조합활성형프로인슐린의분리.정제방법
RU2045535C1 (ru) Способ получения проинсулина человека
CN112300267A (zh) 一种重组人胰岛素的制备和纯化方法

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20200831