CA2455803A1 - Nucleic acid amplification method - Google Patents

Nucleic acid amplification method Download PDF

Info

Publication number
CA2455803A1
CA2455803A1 CA002455803A CA2455803A CA2455803A1 CA 2455803 A1 CA2455803 A1 CA 2455803A1 CA 002455803 A CA002455803 A CA 002455803A CA 2455803 A CA2455803 A CA 2455803A CA 2455803 A1 CA2455803 A1 CA 2455803A1
Authority
CA
Canada
Prior art keywords
probe
probes
amplification
product
amplification product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002455803A
Other languages
English (en)
French (fr)
Inventor
Ulf Landegren
Mats Gullberg
Mats Nilsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocyclica AB
Landegren Gene Technology AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2455803A1 publication Critical patent/CA2455803A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002455803A 2001-08-03 2002-07-12 Nucleic acid amplification method Abandoned CA2455803A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0118959A GB2378245A (en) 2001-08-03 2001-08-03 Nucleic acid amplification method
GB0118959.6 2001-08-03
PCT/SE2002/001378 WO2003012119A2 (en) 2001-08-03 2002-07-12 Nucleic acid amplification method

Publications (1)

Publication Number Publication Date
CA2455803A1 true CA2455803A1 (en) 2003-02-13

Family

ID=9919749

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002455803A Abandoned CA2455803A1 (en) 2001-08-03 2002-07-12 Nucleic acid amplification method

Country Status (7)

Country Link
US (3) US7320860B2 (OSRAM)
EP (4) EP1423532B1 (OSRAM)
JP (4) JP2004536617A (OSRAM)
AU (1) AU2002314693A1 (OSRAM)
CA (1) CA2455803A1 (OSRAM)
GB (1) GB2378245A (OSRAM)
WO (1) WO2003012119A2 (OSRAM)

Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2378245A (en) * 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
GB0304371D0 (en) * 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
US7385043B1 (en) * 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
SE0401270D0 (sv) * 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
US7618780B2 (en) * 2004-05-20 2009-11-17 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
US7972858B2 (en) * 2004-08-13 2011-07-05 President And Fellows Of Harvard College Ultra high-throughput opti-nanopore DNA readout platform
AU2005314431B2 (en) * 2004-12-11 2011-02-17 Cytogenix, Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
EP1910537A1 (en) * 2005-06-06 2008-04-16 454 Life Sciences Corporation Paired end sequencing
JP5103398B2 (ja) * 2005-06-06 2012-12-19 ４５４ ライフ サイエンシーズ コーポレイション 両末端配列決定（ｐａｉｒｅｄｅｎｄｓｅｑｕｅｎｃｉｎｇ）
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US7862999B2 (en) 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
WO2008122960A2 (en) * 2007-04-10 2008-10-16 Microvacuum Ltd. Label-free optical detection method
US20080293589A1 (en) * 2007-05-24 2008-11-27 Affymetrix, Inc. Multiplex locus specific amplification
CN101889074A (zh) * 2007-10-04 2010-11-17 哈尔西恩莫尔丘勒公司 采用电子显微镜对核酸聚合物测序
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
FR2924440B1 (fr) 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US20090233809A1 (en) * 2008-03-04 2009-09-17 Affymetrix, Inc. Resequencing methods for identification of sequence variants
ES2637411T3 (es) 2008-12-01 2017-10-13 Laboratory Corporation Of America Holdings Métodos y ensayos para medir p95 Y/O p95 en una muestra y anticuerpos específicos para p95
SG172983A1 (en) 2009-01-15 2011-08-29 Lab Corp America Holdings Methods of determining patient response by measurement of her-3
KR20110120890A (ko) * 2009-01-15 2011-11-04 래버러토리 코포레이션 오브 아메리카 홀딩스 Ｈｅｒ－２ 발현의 측정에 의한 환자 반응의 결정 방법
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
WO2010126614A2 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
WO2011027966A2 (en) * 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
ES2544635T3 (es) 2010-03-01 2015-09-02 Quanterix Corporation Métodos para extender el rango dinámico en ensayos para la detección de moléculas o partículas
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
EP2585593B1 (en) 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
WO2012049316A1 (en) 2010-10-15 2012-04-19 Olink Ab Dynamic range methods
CN101974638B (zh) * 2010-11-10 2012-10-03 华东医学生物技术研究所 连接反应偶联核酸侵入反应与切刻内切酶反应的核酸信号扩增检测方法
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
JP2014506465A (ja) 2011-02-09 2014-03-17 バイオ−ラド ラボラトリーズ，インコーポレイティド 核酸の分析
WO2012110899A2 (en) 2011-02-15 2012-08-23 Bernitz Mats Nilsson Method for localizedin situdetection of mrna
US9556473B2 (en) 2011-02-15 2017-01-31 Leica Biosystems Newcastle Ltd Methods for identifying nucleic acid sequences
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
US10597701B2 (en) 2011-05-11 2020-03-24 Navinci Diagnostics Ab Unfolding proximity probes and methods for the use thereof
WO2013058907A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
EP2820155B1 (en) 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
KR101922124B1 (ko) 2012-08-27 2018-11-26 삼성전자주식회사 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법
EP2920324B1 (en) 2012-11-14 2017-12-27 Olink Bioscience AB Localised rca-based amplification method
WO2014076214A1 (en) 2012-11-14 2014-05-22 Olink Ab Rca reporter probes and their use in detecting nucleic acid molecules
JP6112652B2 (ja) * 2012-11-29 2017-04-12 国立大学法人広島大学 標的ｄｎａの特異的増幅方法
EP2959019B1 (en) * 2013-02-20 2019-09-04 Emory University Methods of sequencing nucleic acids in mixtures and compositions related thereto
KR102257912B1 (ko) 2013-03-13 2021-05-27 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
US8778609B1 (en) 2013-03-14 2014-07-15 Good Start Genetics, Inc. Methods for analyzing nucleic acids
MX347461B (es) 2013-03-15 2017-04-26 Lubrizol Advanced Mat Inc Composiciones de cpvc libres de metales pesados.
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
EP3024948B1 (en) 2013-07-25 2020-01-15 Bio-rad Laboratories, Inc. Genetic assays for detecting viral recombination rate
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
GB201320145D0 (en) 2013-11-14 2014-01-01 Olink Ab Localised RCA-based amplification method using a padlock-probe
GB201321123D0 (en) * 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
US10112194B2 (en) 2014-04-14 2018-10-30 Q-Linea Ab Detection of microscopic objects
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
CN119709943A (zh) 2014-05-15 2025-03-28 中尺度技术有限责任公司 改进的测定方法
AU2015273443B2 (en) 2014-06-13 2021-03-04 Q-Linea Ab Method for detecting and characterising a microorganism
GB201413717D0 (en) 2014-08-01 2014-09-17 Olink Ab Method for selecting a target nucleic acid sequence
GB201413718D0 (en) 2014-08-01 2014-09-17 Olink Ab Method for selecting a target nucleic acid sequence
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
EP4098753A1 (en) 2014-10-23 2022-12-07 Ricardo Mancebo Reagents and methods for isothermal chain reaction
US10066259B2 (en) 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
EP3303377B1 (en) 2015-06-05 2022-11-23 Dana-Farber Cancer Institute, Inc. Compositions and methods for transient gene therapy with enhanced stability
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
GB201511129D0 (en) 2015-06-24 2015-08-05 Linea Ab Q Method of determining antimicrobial susceptibility of a microorganism
US10465245B2 (en) 2015-07-29 2019-11-05 Progenity, Inc. Nucleic acids and methods for detecting chromosomal abnormalities
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
CA3005101A1 (en) 2015-11-16 2017-05-26 Progenity, Inc. Nucleic acids and methods for detecting methylation status
WO2017173279A1 (en) * 2016-03-31 2017-10-05 Arizona Board Of Regents On Behalf Of Arizona State University Probes and methods for measuring tandem repeats
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
US10655170B2 (en) 2016-07-06 2020-05-19 Takara Bio Usa, Inc. Coupling adaptors to a target nucleic acid
GB201614023D0 (en) 2016-08-16 2016-09-28 Olink Bioscience Ab Double-stranded circle probes
GB201621514D0 (en) 2016-12-16 2017-02-01 Q-Linea Ab Padlock probe detection method
EP3601610A1 (en) 2017-03-30 2020-02-05 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
US11753434B2 (en) 2017-04-14 2023-09-12 Dana-Farber Cancer Institute, Inc. Compositions and methods for transient gene therapy with enhanced stability
US11459603B2 (en) * 2017-05-23 2022-10-04 Rutgers, The State University Of New Jersey Target mediated in situ signal amplification with dual interacting hairpin probes
EP3682025A1 (en) 2017-09-14 2020-07-22 H. Hoffnabb-La Roche Ag Novel method for generating circular single-stranded dna libraries
EP3682027B1 (en) 2017-09-15 2025-02-26 F. Hoffmann-La Roche AG Hybridization-extension-ligation strategy for generating circular single-stranded dna libraries
WO2019068797A1 (en) 2017-10-06 2019-04-11 F. Hoffmann-La Roche Ag CIRCULARIZATION METHODS FOR THE PREPARATION OF SAMPLE SAMPLES OF UNIQUE MOLECULES
EP4589017A3 (en) 2017-10-06 2025-10-08 10x Genomics, Inc. Rna templated ligation
WO2019086531A1 (en) 2017-11-03 2019-05-09 F. Hoffmann-La Roche Ag Linear consensus sequencing
CN111801427B (zh) 2018-02-05 2023-12-05 豪夫迈·罗氏有限公司 用于单分子的单链环状dna模板的产生
EP3759251A1 (en) 2018-03-02 2021-01-06 H. Hoffnabb-La Roche Ag Generation of double-stranded dna templates for single molecule sequencing
CA3095292A1 (en) 2018-04-02 2019-10-10 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
CN109444102B (zh) * 2018-12-18 2021-03-23 济南大学 一种检测赭曲霉毒素a的荧光生物传感器及其制备方法和应用
CN120098057A (zh) 2019-03-01 2025-06-06 中尺度技术有限责任公司 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
WO2021113290A1 (en) 2019-12-03 2021-06-10 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
GB201919029D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
WO2022032194A1 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
WO2022056078A1 (en) * 2020-09-11 2022-03-17 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Rnase h-assisted detection assay for rna (radar)
CN116438454A (zh) 2020-09-21 2023-07-14 普罗根尼蒂公司 用于分离细胞游离dna的组合物和方法
US12497651B2 (en) 2020-11-23 2025-12-16 Pleno, Inc. Encoded dual-probe endonuclease assays
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
GB202203185D0 (en) 2022-03-08 2022-04-20 Rarity Bioscience Ab Method for detecting nucleic acid amplification products
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
GB202310278D0 (en) 2023-07-05 2023-08-16 Navinci Diagnostics Ab Stabilisation of phi29 polymerase
CN120249442B (zh) * 2025-06-04 2025-09-26 北京航空航天大学杭州创新研究院 一种基于邻近核酸扩增信号放大的超灵敏免疫检测方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
JPH06500014A (ja) * 1990-07-25 1994-01-06 シンジーン，インコーポレイテッド 多数の核酸相補体を生成させる環状伸長法
JP3085409B2 (ja) 1991-03-29 2000-09-11 東洋紡績株式会社 標的核酸配列の検出方法およびそのための試薬キット
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
EP1260593A3 (en) 1994-12-23 2003-12-03 Dade Behring Inc. Detection of nucleic acids by nuclease-catalyzed product formation
SE504798C2 (sv) 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
US6143495A (en) 1995-11-21 2000-11-07 Yale University Unimolecular segment amplification and sequencing
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1997020948A1 (en) 1995-12-05 1997-06-12 Koch Joern Erland A cascade nucleic acid amplification reaction
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
WO1999049079A1 (en) 1998-03-25 1999-09-30 Ulf Landegren Rolling circle replication of padlock probes
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6150112A (en) 1998-09-18 2000-11-21 Yale University Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
JP4163386B2 (ja) 1998-12-15 2008-10-08 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 環状化核酸プローブの増幅方法
US6830884B1 (en) * 1998-12-15 2004-12-14 Molecular Staging Inc. Method of amplification
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
CA2392474A1 (en) 1999-11-26 2001-05-31 Jonathan M. Rothberg Nucleic acid probe arrays
AU1804001A (en) 1999-12-02 2001-06-12 Molecular Staging, Inc. Generation of single-strand circular dna from linear self-annealing segments
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
US6291187B1 (en) * 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
GB2378245A (en) * 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel

Also Published As

Publication number Publication date
JP2010183916A (ja) 2010-08-26
WO2003012119A2 (en) 2003-02-13
US7320860B2 (en) 2008-01-22
JP5313204B2 (ja) 2013-10-09
EP2236622A2 (en) 2010-10-06
WO2003012119A3 (en) 2003-11-13
AU2002314693A1 (en) 2003-02-17
EP1423532B1 (en) 2013-05-22
EP2236622A3 (en) 2010-11-03
EP1423532A2 (en) 2004-06-02
US20050287526A1 (en) 2005-12-29
GB2378245A (en) 2003-02-05
JP4559517B2 (ja) 2010-10-06
EP2236622B1 (en) 2017-09-06
EP2251439A1 (en) 2010-11-17
JP2004536617A (ja) 2004-12-09
JP2009077735A (ja) 2009-04-16
EP2251439B1 (en) 2013-09-11
JP2009297045A (ja) 2009-12-24
US20080131899A1 (en) 2008-06-05
JP4675424B2 (ja) 2011-04-20
EP2224016A1 (en) 2010-09-01
GB0118959D0 (en) 2001-09-26
US7790388B2 (en) 2010-09-07
USRE44265E1 (en) 2013-06-04

Similar Documents

Publication Publication Date Title
US7320860B2 (en) Nucleic acid amplification method
US20220213535A1 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay
KR20120105811A (ko) Ｐｔｏ 절단 및 연장-의존적 시그널 발생에 의한 타겟 핵산서열의 검출
AU2015203677B2 (en) Detection of Target Nucleic Acid Sequences by PTO Cleavage and Extension
NZ706144B2 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay
NZ604338B2 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued