CA2441820C - Evolving new molecular function - Google Patents

Evolving new molecular function Download PDF

Info

Publication number
CA2441820C
CA2441820C CA2441820A CA2441820A CA2441820C CA 2441820 C CA2441820 C CA 2441820C CA 2441820 A CA2441820 A CA 2441820A CA 2441820 A CA2441820 A CA 2441820A CA 2441820 C CA2441820 C CA 2441820C
Authority
CA
Canada
Prior art keywords
template
reaction product
dna
oligonucleotide
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2441820A
Other languages
English (en)
French (fr)
Other versions
CA2441820A1 (en
Inventor
David R. Liu
Zev J. Gartner
Mattew W. Kanan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27493169&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2441820(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Publication of CA2441820A1 publication Critical patent/CA2441820A1/en
Application granted granted Critical
Publication of CA2441820C publication Critical patent/CA2441820C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
CA2441820A 2001-03-19 2002-03-19 Evolving new molecular function Expired - Fee Related CA2441820C (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US27708101P 2001-03-19 2001-03-19
US27709401P 2001-03-19 2001-03-19
US60/277,094 2001-03-19
US60/277,081 2001-03-19
US30669101P 2001-07-20 2001-07-20
US60/306,691 2001-07-20
US10/101,030 US7070928B2 (en) 2001-03-19 2002-03-19 Evolving new molecular function
US10/101,030 2002-03-19
PCT/US2002/008546 WO2002074929A2 (en) 2001-03-19 2002-03-19 Evolving new molecular function

Publications (2)

Publication Number Publication Date
CA2441820A1 CA2441820A1 (en) 2002-09-26
CA2441820C true CA2441820C (en) 2014-01-07

Family

ID=27493169

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2441820A Expired - Fee Related CA2441820C (en) 2001-03-19 2002-03-19 Evolving new molecular function

Country Status (12)

Country Link
US (7) US7070928B2 (US20050042669A1-20050224-C00003.png)
EP (3) EP2332896A3 (US20050042669A1-20050224-C00003.png)
JP (4) JP4128453B2 (US20050042669A1-20050224-C00003.png)
AT (1) ATE335754T1 (US20050042669A1-20050224-C00003.png)
AU (2) AU2002306777C1 (US20050042669A1-20050224-C00003.png)
CA (1) CA2441820C (US20050042669A1-20050224-C00003.png)
DE (1) DE60213826T3 (US20050042669A1-20050224-C00003.png)
DK (1) DK1423400T4 (US20050042669A1-20050224-C00003.png)
ES (1) ES2271306T5 (US20050042669A1-20050224-C00003.png)
IL (4) IL158000A0 (US20050042669A1-20050224-C00003.png)
PT (1) PT1423400E (US20050042669A1-20050224-C00003.png)
WO (1) WO2002074929A2 (US20050042669A1-20050224-C00003.png)

Families Citing this family (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US7070928B2 (en) * 2001-03-19 2006-07-04 President And Fellows Of Harvard College Evolving new molecular function
EP1402024B1 (en) * 2001-06-20 2007-08-22 Nuevolution A/S Templated molecules and methods for using such molecules
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US8073627B2 (en) * 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt Mfd Gmbh & Co Kg Festwalzgerät einer Festwalzmaschine für Kurbelwellen
DK1483585T3 (da) 2002-03-08 2009-06-22 Eidgenoess Tech Hochschule Kodede, selvsamlende kemiske biblioteker (ESACHEL)
AU2003218629A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
IL163822A0 (en) * 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
WO2003078050A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c-c bond upon reaction
AU2003218630A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block capable of functional entity transfer to nucleophil
WO2004013070A2 (en) * 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
AU2003263937B2 (en) * 2002-08-19 2010-04-01 The President And Fellows Of Harvard College Evolving new molecular function
WO2004024929A2 (en) * 2002-09-12 2004-03-25 Nuevolution A/S Proximity-aided synthesis of templated molecules
WO2004028682A2 (en) 2002-09-27 2004-04-08 Carlsberg A/S Spatially encoded polymer matrix
AU2011226815B2 (en) * 2002-10-30 2014-09-25 Nuevolution A/S Enzymatic encoding
PT1558744E (pt) 2002-10-30 2011-09-22 Nuevolution As Codificação enzimática
ATE450609T1 (de) 2002-12-19 2009-12-15 Nuevolution As Durch quasizufallsstrukturen und funktionen geführte synthesemethode
EP1597395A2 (en) * 2003-02-21 2005-11-23 Nuevolution A/S Method for producing second-generation library
WO2004074501A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S A method for obtaining structural information about an encoded molecule
ATE424455T1 (de) * 2003-03-20 2009-03-15 Nuevolution As Ligationsvermittelnde codierung von kleinen molekülen
US8017323B2 (en) * 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
WO2005016851A1 (en) * 2003-05-08 2005-02-24 Valent Technologies, Llc Recombined molecules and preparation thereof
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
WO2004110964A2 (en) * 2003-06-16 2004-12-23 Nuevolution A/S Encoded molecules by translation (emt)
WO2005003778A2 (en) * 2003-07-02 2005-01-13 Nuevolution A/S A method for identifying a synthetic molecule having affinity towards a target
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122099A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
EP1670939B1 (en) 2003-09-18 2009-11-04 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
WO2005058479A2 (en) 2003-12-17 2005-06-30 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
WO2005078122A2 (en) 2004-02-17 2005-08-25 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
ATE447020T1 (de) * 2004-03-22 2009-11-15 Nuevolution As Ligationscodierung unter verwendung von oligonukleotidbausteinen
EP2458619B1 (en) 2004-05-24 2017-08-02 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
CA2565967A1 (en) 2004-06-10 2005-12-29 Perkinelmer Las, Inc. Multiplexing assays for analyte detection
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
CA2587010C (en) 2004-11-08 2017-08-15 Vipergen Aps Structural nucleic acid guided chemical synthesis
DK1828381T3 (da) 2004-11-22 2009-02-23 Peter Birk Rasmussen Templatedirigeret split-and-mix-syntese af småmolekylebiblioteker
US20060205040A1 (en) 2005-03-03 2006-09-14 Rangarajan Sampath Compositions for use in identification of adventitious viruses
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
CA2605021A1 (en) * 2005-04-13 2007-08-02 Isis Pharmaceuticals, Inc. Compositions for use in identification of adenoviruses
EP1885892A2 (en) * 2005-05-03 2008-02-13 Ensemble Discovery Corporation Turnover probes and use thereof for nucleic acid detection
US20070154899A1 (en) * 2005-05-26 2007-07-05 Coull James M Biodetection by nucleic acid-templated chemistry
US20090203530A1 (en) * 2005-06-07 2009-08-13 Liu David R Polymer evolution via templated synthesis
CA2611512C (en) 2005-06-09 2018-05-22 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US20090035824A1 (en) * 2005-06-17 2009-02-05 Liu David R Nucleic acid-templated chemistry in organic solvents
DE602006013134D1 (de) * 2005-06-17 2010-05-06 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
WO2007011722A2 (en) 2005-07-15 2007-01-25 President And Fellows Of Harvard College Reaction discovery system
AU2006272776B2 (en) 2005-07-21 2012-01-19 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
EP1785490B1 (en) * 2005-10-17 2007-11-14 RiNA Netzwerk RNA-Technologien GmbH Method for determining an unknown PNA sequence and uses thereof
EP1940755A2 (en) 2005-10-28 2008-07-09 Praecis Pharmaceuticals Inc. Methods for identifying compounds of interest using encoded libraries
DE602006018648D1 (de) 2005-12-01 2011-01-13 Nuevolution As Enzymvermittelnde kodierungsmethoden für eine effiziente synthese von grossen bibliotheken
US7928211B2 (en) 2006-05-03 2011-04-19 Vipergen Pharmaceuticals Aps Method for preparing compounds by nucleic acid directed synthesis
AU2007353877B2 (en) 2006-09-14 2012-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
WO2008036273A2 (en) * 2006-09-18 2008-03-27 Ensemble Discovery Corporation Receptor family profiling
CA2664649A1 (en) 2006-09-28 2008-05-08 Ensemble Discovery Corporation Compositions and methods for biodetection by nucleic acid-templated chemistry
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的dna分析法
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
CN101952719A (zh) * 2007-07-27 2011-01-19 通信改革公司 检测测定法及其用途
US20130217588A1 (en) * 2008-01-18 2013-08-22 The Johns Hopkins University Methods for Identifying Eubacteria
US8338565B2 (en) * 2008-08-20 2012-12-25 Ensemble Therapeutics Corporation Macrocyclic compounds for inhibition of tumor necrosis factor alpha
WO2010031002A1 (en) * 2008-09-14 2010-03-18 Incitor, Llc Nanoscale molecule synthesis
EP2344893B1 (en) 2008-09-16 2014-10-15 Ibis Biosciences, Inc. Microplate handling systems and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
EP2349549B1 (en) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, and system
EP2396803A4 (en) 2009-02-12 2016-10-26 Ibis Biosciences Inc IONIZATION PROBE ASSEMBLIES
SG2014011381A (en) 2009-02-13 2014-06-27 Chem Inc X Methods of creating and screening dna-encoded libraries
WO2010150103A2 (en) 2009-06-22 2010-12-29 Universite De Strasbourg Method of preparing an adduct
WO2011008972A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systems for bioagent identification
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
ES2628739T3 (es) 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Amplificación por desplazamiento múltiple
ES2713873T3 (es) 2010-04-16 2019-05-24 Nuevolution As Complejos bifuncionales y métodos para hacer y utilizar tales complejos
EP2622073B1 (en) 2010-09-27 2018-04-18 Vipergen A method for making an enriched library
CN103717751A (zh) * 2011-05-19 2014-04-09 塞昆纳姆股份有限公司 用于多重核酸鉴定的产品和方法
EP3828271A1 (en) 2011-09-07 2021-06-02 X-Chem, Inc. Methods for tagging dna-encoded libraries
EA034141B1 (ru) 2012-07-13 2020-01-09 Икс-Чем, Инк. Днк-кодированные библиотеки, содержащие связи кодирующих олигонуклеотидов, не доступные для считывания полимеразами
EP2971280B1 (en) 2013-03-15 2018-08-29 Arizona Board of Regents on behalf of Arizona State University Biosensor microarray compositions and methods
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
GB201322692D0 (en) 2013-12-20 2014-02-05 Philochem Ag Production of encoded chemical libraries
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
US20180340174A1 (en) 2014-11-11 2018-11-29 Nanocore Aps Method for identification of molecules with desired characteristics
WO2016094845A2 (en) * 2014-12-12 2016-06-16 Woolf Tod M Compositions and methods for editing nucleic acids in cells utilizing oligonucleotides
WO2016172571A1 (en) 2015-04-24 2016-10-27 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
JP7283727B2 (ja) 2016-06-16 2023-05-30 ヘイスタック サイエンシィズ コーポレーション コードプローブ分子のコンビナトリアル合成が指令されかつ記録されたオリゴヌクレオチド
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018081534A1 (en) 2016-10-28 2018-05-03 President And Fellows Of Harvard College Assay for exo-site binding molecules
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation MOLECULES FOR THE VERIFICATION OF OLIGONUCLEOTIDE DIRECTED COMBINATIONAL SYNTHESIS AND METHOD OF MANUFACTURING AND USING THEREOF
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
GB2582865B (en) * 2017-09-11 2022-05-04 Synthego Corp Biopolymer synthesis system and method
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019168654A2 (en) 2018-02-09 2019-09-06 President And Fellows Of Harvard College Dna-templated macrocycle library
WO2020124213A1 (en) 2018-12-20 2020-06-25 Krylov Sergey N Binder selection using capillary electrophoresis
WO2020152028A1 (en) 2019-01-22 2020-07-30 Vipergen Aps A method for screening of an in vitro display library within a cell
JP2022526908A (ja) 2019-03-19 2022-05-27 ザ ブロード インスティテュート,インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
AU2021267940A1 (en) 2020-05-08 2022-12-08 President And Fellows Of Harvard College Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
WO2023164224A1 (en) * 2022-02-28 2023-08-31 The Board Of Trustees Of The Leland Stanford Junior University Functionalized nanoclusters and their use in treating bacterial infections

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US277081A (en) 1883-05-08 Scalding napped hats and sizing or felting hat and other fabrics
US4194550A (en) * 1976-01-26 1980-03-25 Insulating Shade (Limited Partnership) Apparatus for insulating against conductive, convective and radiant heat transmission
US4863857A (en) * 1985-03-01 1989-09-05 Board Of Regents, The University Of Texas System Polypeptide complementary to peptides or proteins having an amino acid sequence or nucleotide coding sequence at least partially known
US5506337A (en) * 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4703034A (en) * 1986-04-28 1987-10-27 Merck & Co., Inc. Novel cyclic tetrapeptide
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5162218A (en) * 1988-11-18 1992-11-10 The Regents Of The University Of California Conjugated polypeptides and methods for their preparation
US6680192B1 (en) * 1989-05-16 2004-01-20 Scripps Research Institute Method for producing polymers having a preselected activity
US6291161B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
US6291160B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
CA2067194C (en) 1989-10-05 2003-03-18 Glenn Kawasaki Cell-free synthesis and isolation of novel genes and polypeptides
US5637682A (en) 1990-06-11 1997-06-10 Nexstar Pharmaceuticals, Inc. High-affinity oligonucleotide ligands to the tachykinin substance P
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5723289A (en) * 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
US20020038000A1 (en) 1990-08-02 2002-03-28 Larry Gold Systematic polypeptide evolution by reverse translation
US5843701A (en) * 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
WO1992002536A1 (en) 1990-08-02 1992-02-20 The Regents Of The University Of Colorado Systematic polypeptide evolution by reverse translation
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US6140496A (en) * 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5719262A (en) * 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5641625A (en) * 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
ATE163308T1 (de) * 1991-08-28 1998-03-15 Boehringer Mannheim Gmbh Primer für matrizenabhängige enzymatische nukleinsäuresynthesen
CA2118806A1 (en) 1991-09-18 1993-04-01 William J. Dower Method of synthesizing diverse collections of oligomers
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
WO1993009668A1 (en) 1991-11-22 1993-05-27 Affymax Technology N.V. Combinatorial strategies for polymer synthesis
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5721099A (en) * 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
DE69433811T2 (de) 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
AU687801B2 (en) 1993-03-19 1998-03-05 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5840485A (en) * 1993-05-27 1998-11-24 Selectide Corporation Topologically segregated, encoded solid phase libraries
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5922545A (en) * 1993-10-29 1999-07-13 Affymax Technologies N.V. In vitro peptide and antibody display libraries
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
AU8102694A (en) 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
US6127154A (en) * 1994-02-10 2000-10-03 Mosbach; Klaus Methods for direct synthesis of compounds having complementary structure to a desired molecular entity and use thereof
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6048698A (en) * 1994-09-20 2000-04-11 Nexstar Pharmaceuticals, Inc. Parallel SELEX™
US20030099945A1 (en) * 1994-09-20 2003-05-29 Invenux, Inc. Parallel selex
US5597697A (en) * 1994-09-30 1997-01-28 Diamond; Paul Screening assay for inhibitors and activators of RNA and DNA-dependent nucleic acid polymerases
GB9524630D0 (en) * 1994-12-24 1996-01-31 Zeneca Ltd Chemical compounds
US5559000A (en) * 1995-01-18 1996-09-24 The Scripps Research Institute Encoded reaction cassette
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
US5846839A (en) 1995-12-22 1998-12-08 Glaxo Group Limited Methods for hard-tagging an encoded synthetic library
US5888971A (en) * 1996-02-20 1999-03-30 Ortho Pharmaceutical Corporation, Inc. Macrocyclic peptides useful in the treatment of thrombin related disorders
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
US5998140A (en) * 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6080826A (en) * 1997-01-06 2000-06-27 California Institute Of Technology Template-directed ring-closing metathesis and ring-opening metathesis polymerization of functionalized dienes
WO1998030575A1 (en) * 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
ATE332368T1 (de) * 1997-01-21 2006-07-15 Gen Hospital Corp Selektion von proteinen mittels rns-protein fusionen
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
US6969584B2 (en) 1997-06-12 2005-11-29 Rigel Pharmaceuticals, Inc. Combinatorial enzymatic complexes
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
EP1051515A2 (en) 1998-01-27 2000-11-15 Cytocell Limited Modified nucleic acid probes and uses thereof
US5945325A (en) 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6175001B1 (en) * 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
AU1318400A (en) * 1998-10-19 2000-05-08 Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
EP1208219A1 (en) * 1999-04-08 2002-05-29 Pavel Sergeev Synthesis of biologically active compounds in cells
KR20020022808A (ko) * 1999-08-12 2002-03-27 리우, 루 조합 화학 및 의약 화학용 효소의 진화 및 용도
US6436665B1 (en) * 1999-08-27 2002-08-20 Phylos, Inc Methods for encoding and sorting in vitro translated proteins
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US7070928B2 (en) 2001-03-19 2006-07-04 President And Fellows Of Harvard College Evolving new molecular function
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US20030143561A1 (en) * 2001-06-20 2003-07-31 Nuevolution A/S Nucleoside derivatives for library preparation
EP1402024B1 (en) 2001-06-20 2007-08-22 Nuevolution A/S Templated molecules and methods for using such molecules
WO2003078445A2 (en) 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c=c double bond upon reaction
DK1483585T3 (da) 2002-03-08 2009-06-22 Eidgenoess Tech Hochschule Kodede, selvsamlende kemiske biblioteker (ESACHEL)
AU2003218630A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block capable of functional entity transfer to nucleophil
AU2003218629A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
WO2003078050A2 (en) 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c-c bond upon reaction
IL163822A0 (en) 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
US7255991B2 (en) 2002-03-22 2007-08-14 Emory University Template-drive processes for synthesizing polymers and components related to such processes
AU2003240436A1 (en) 2002-06-20 2004-01-06 Nuevolution A/S Microarrays displaying encoded molecules
AU2003263937B2 (en) * 2002-08-19 2010-04-01 The President And Fellows Of Harvard College Evolving new molecular function
US8017323B2 (en) 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
ATE461279T1 (de) * 2005-06-07 2010-04-15 Harvard College Geordnete mehrschrittsynthese mittels nukleinsäurevermittelter chemie
DE602006013134D1 (de) 2005-06-17 2010-05-06 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
US20090035824A1 (en) * 2005-06-17 2009-02-05 Liu David R Nucleic acid-templated chemistry in organic solvents

Also Published As

Publication number Publication date
JP4384229B2 (ja) 2009-12-16
US20030113738A1 (en) 2003-06-19
US20050233381A1 (en) 2005-10-20
IL206586A0 (en) 2010-12-30
US20050025766A1 (en) 2005-02-03
DK1423400T3 (da) 2006-12-11
EP1423400A2 (en) 2004-06-02
WO2002074929A2 (en) 2002-09-26
PT1423400E (pt) 2006-12-29
JP2009232869A (ja) 2009-10-15
IL158000A (en) 2014-08-31
DE60213826T3 (de) 2013-10-17
ES2271306T3 (es) 2007-04-16
US20050042669A1 (en) 2005-02-24
US20050227281A1 (en) 2005-10-13
EP2332896A2 (en) 2011-06-15
IL206585A (en) 2015-04-30
AU2002306777B2 (en) 2007-10-04
EP1832567A3 (en) 2009-08-26
JP2004536162A (ja) 2004-12-02
US7223545B2 (en) 2007-05-29
IL206586A (en) 2012-12-31
EP1423400B1 (en) 2006-08-09
CA2441820A1 (en) 2002-09-26
US20050281819A9 (en) 2005-12-22
EP1423400A4 (en) 2004-12-01
ATE335754T1 (de) 2006-09-15
DE60213826D1 (de) 2006-09-21
US7442160B2 (en) 2008-10-28
US7998904B2 (en) 2011-08-16
IL158000A0 (en) 2004-03-28
DK1423400T4 (da) 2013-09-02
WO2002074929A3 (en) 2003-05-30
ES2271306T5 (es) 2013-10-16
DE60213826T8 (de) 2007-08-30
IL206585A0 (en) 2010-12-30
JP4128453B2 (ja) 2008-07-30
DE60213826T2 (de) 2007-03-29
US20050142583A1 (en) 2005-06-30
US7070928B2 (en) 2006-07-04
JP2008183010A (ja) 2008-08-14
JP5509270B2 (ja) 2014-06-04
EP1832567A2 (en) 2007-09-12
EP2332896A3 (en) 2012-09-26
JP2013010761A (ja) 2013-01-17
AU2002306777C1 (en) 2008-04-24
AU2008200027B2 (en) 2010-05-27
US7557068B2 (en) 2009-07-07
AU2008200027A1 (en) 2008-01-31
US20120165228A1 (en) 2012-06-28
EP1423400B2 (en) 2013-05-22

Similar Documents

Publication Publication Date Title
CA2441820C (en) Evolving new molecular function
AU2002306777A1 (en) Evolving new molecular function
JP4657919B2 (ja) 進化する新しい分子機能
JP2005536234A5 (US20050042669A1-20050224-C00003.png)

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20200831