CA2198899C - Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires - Google Patents

Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires Download PDF

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Publication number
CA2198899C
CA2198899C CA2198899A CA2198899A CA2198899C CA 2198899 C CA2198899 C CA 2198899C CA 2198899 A CA2198899 A CA 2198899A CA 2198899 A CA2198899 A CA 2198899A CA 2198899 C CA2198899 C CA 2198899C
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Prior art keywords
light chain
seq
immunoglobulin
sequence
heavy
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Expired - Lifetime
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CA2198899A
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CA2198899A1 (fr
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Carlos F. Barbas
Dennis R. Burton
Richard A. Lerner
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Scripps Research Institute
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Scripps Research Institute
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/10—Organic compounds
    • A61K49/14—Peptides, e.g. proteins
    • A61K49/16—Antibodies; Immunoglobulins; Fragments thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • AIDS & HIV (AREA)
  • Optics & Photonics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)

Abstract

La présente invention concerne des procédés permettant de produire des banques d'anticorps, et particulièrement d'augmenter la diversité de banques d'anticorps par induction d'une mutagenèse dans les régions déterminantes complémentaires (CDR) de chaînes lourdes ou légères d'immunoglobuline qui apparaissent à la surface de particules de phages filamenteux, comprenant la banque. L'invention concerne également des oligonucléotides utilisés pour augmenter la diversité des banques, ainsi que des chaînes légères universelles utilisées dans lesdits procédés de production de banques d'anticorps.
CA2198899A 1994-09-02 1995-09-01 Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires Expired - Lifetime CA2198899C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/300,386 US5667988A (en) 1992-01-27 1994-09-02 Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
US08/300,386 1994-09-02
PCT/US1995/011235 WO1996007754A1 (fr) 1994-09-02 1995-09-01 Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires

Publications (2)

Publication Number Publication Date
CA2198899A1 CA2198899A1 (fr) 1996-03-14
CA2198899C true CA2198899C (fr) 2010-02-23

Family

ID=23158889

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2198899A Expired - Lifetime CA2198899C (fr) 1994-09-02 1995-09-01 Procedes de production de banques d'anticorps au moyen de chaines legeres d'immunoglobuline universelles ou rendues alleatoires

Country Status (11)

Country Link
US (2) US5667988A (fr)
EP (1) EP0779933B1 (fr)
JP (1) JPH10504970A (fr)
AT (1) ATE236992T1 (fr)
AU (1) AU706343B2 (fr)
CA (1) CA2198899C (fr)
DE (1) DE69530305T2 (fr)
DK (1) DK0779933T3 (fr)
ES (1) ES2196077T3 (fr)
PT (1) PT779933E (fr)
WO (1) WO1996007754A1 (fr)

Families Citing this family (359)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6955900B1 (en) * 1993-02-02 2005-10-18 The Scripps Research Institute Methods for producing polypeptide binding sites, monoclonal antibodies and compositions thereof
US20060078561A1 (en) * 1994-01-31 2006-04-13 The Trustees Of Boston University Polyclonal antibody libraries
ATE243745T1 (de) 1994-01-31 2003-07-15 Univ Boston Bibliotheken aus polyklonalen antikörpern
JP4436457B2 (ja) * 1995-08-18 2010-03-24 モルフォシス アイピー ゲーエムベーハー 蛋白質／（ポリ）ペプチドライブラリー
US6017754A (en) * 1995-08-24 2000-01-25 Invitrogen Corporation System for isolating and identifying eukaryotic cells transfected with genes and vectors, host cells and methods thereof
FR2741892B1 (fr) * 1995-12-04 1998-02-13 Pasteur Merieux Serums Vacc Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps, banque et systemes d'expression d'anticorps "coliclonaux" obtenus
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
GB9701425D0 (en) 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6596850B1 (en) * 1998-01-30 2003-07-22 Ixsys, Incorporated Anti-αv3β3 recombinant human antibodies, nucleic acids encoding same
US6590079B2 (en) * 1997-01-30 2003-07-08 Ixsys, Incorporated Anti-αvβ3 recombinant human antibodies, nucleic acids encoding same
JP2002514919A (ja) * 1997-04-04 2002-05-21 バイオサイト ダイアグノスティックス，インコーポレイテッド 多価ライブラリーおよびポリクローナルライブラリー
US6057098A (en) 1997-04-04 2000-05-02 Biosite Diagnostics, Inc. Polyvalent display libraries
GB9722131D0 (en) * 1997-10-20 1997-12-17 Medical Res Council Method
US6759243B2 (en) * 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
EP2311490A3 (fr) 1998-07-13 2011-05-04 Board of Regents, The University of Texas System L'utilisation d'anticorps vis-à-vis des aminophospholipides pour le traitement du cancer
AU5122499A (en) 1998-07-27 2000-02-21 Genentech Inc. Improved transformation efficiency in phage display through modification of a coat protein
US6531580B1 (en) * 1999-06-24 2003-03-11 Ixsys, Inc. Anti-αvβ3 recombinant human antibodies and nucleic acids encoding same
US7135287B1 (en) 1999-10-02 2006-11-14 Biosite, Inc. Human antibodies
US20070111259A1 (en) * 1999-10-02 2007-05-17 Medarex, Inc. Human antibodies
US6794132B2 (en) 1999-10-02 2004-09-21 Biosite, Inc. Human antibodies
US6849425B1 (en) 1999-10-14 2005-02-01 Ixsys, Inc. Methods of optimizing antibody variable region binding affinity
US6680209B1 (en) 1999-12-06 2004-01-20 Biosite, Incorporated Human antibodies as diagnostic reagents
US20030180714A1 (en) * 1999-12-15 2003-09-25 Genentech, Inc. Shotgun scanning
US7229619B1 (en) 2000-11-28 2007-06-12 Medimmune, Inc. Methods of administering/dosing anti-RSV antibodies for prophylaxis and treatment
ATE474854T1 (de) * 2000-01-27 2010-08-15 Medimmune Llc Rsv neutralisierende antikörper mit sehr hohen affinität
CA2401652A1 (fr) 2000-03-01 2001-09-07 Medimmune, Inc. Anticorps de recombinaison hautement efficaces et procede de production de ceux-ci
UA81743C2 (uk) 2000-08-07 2008-02-11 Центокор, Инк. МОНОКЛОНАЛЬНЕ АНТИТІЛО ЛЮДИНИ, ЩО СПЕЦИФІЧНО ЗВ'ЯЗУЄТЬСЯ З ФАКТОРОМ НЕКРОЗУ ПУХЛИН АЛЬФА (ФНПα), ФАРМАЦЕВТИЧНА КОМПОЗИЦІЯ, ЩО ЙОГО МІСТИТЬ, ТА СПОСІБ ЛІКУВАННЯ РЕВМАТОЇДНОГО АРТРИТУ
US6902734B2 (en) 2000-08-07 2005-06-07 Centocor, Inc. Anti-IL-12 antibodies and compositions thereof
US7288390B2 (en) 2000-08-07 2007-10-30 Centocor, Inc. Anti-dual integrin antibodies, compositions, methods and uses
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6818216B2 (en) * 2000-11-28 2004-11-16 Medimmune, Inc. Anti-RSV antibodies
US7179900B2 (en) * 2000-11-28 2007-02-20 Medimmune, Inc. Methods of administering/dosing anti-RSV antibodies for prophylaxis and treatment
US6855493B2 (en) 2000-11-28 2005-02-15 Medimmune, Inc. Methods of administering/dosing anti-RSV antibodies for prophylaxis and treatment
US7087726B2 (en) 2001-02-22 2006-08-08 Genentech, Inc. Anti-interferon-α antibodies
EP1493028A4 (fr) * 2001-07-06 2006-06-14 Genentech Inc Ligands du domaine pdz exprimes a la surface des phages
US20030104604A1 (en) * 2001-08-03 2003-06-05 Weiping Yang Genetically engineered bacterial strains for the display of foreign peptides on filamentous phage
DE60232688D1 (de) 2001-11-20 2009-07-30 Univ Duke Grenzflächen-biomaterialien
GB0202206D0 (en) * 2002-01-31 2002-03-20 Bioinvent Int Ab Method of making libraries of anti-ligands
US7504364B2 (en) * 2002-03-01 2009-03-17 Receptors Llc Methods of making arrays and artificial receptors
US7244592B2 (en) * 2002-03-07 2007-07-17 Dyax Corp. Ligand screening and discovery
WO2003088911A2 (fr) * 2002-04-17 2003-10-30 Roberto Crea Bibliotheques universelles pour immunoglobulines
US7405062B2 (en) * 2002-05-14 2008-07-29 San Diego Antibody Method for cloning variable domain sequences of immunological gene repertoire
EP1513879B1 (fr) * 2002-06-03 2018-08-22 Genentech, Inc. Bibliotheques de phages et anticorps synthetiques
US7425618B2 (en) * 2002-06-14 2008-09-16 Medimmune, Inc. Stabilized anti-respiratory syncytial virus (RSV) antibody formulations
US7132100B2 (en) 2002-06-14 2006-11-07 Medimmune, Inc. Stabilized liquid anti-RSV antibody formulations
CA2491310C (fr) 2002-07-15 2015-10-06 Board Of Regents, The University Of Texas System Compositions comprenant des derives de la duramycine impermeants vis-a-vis des parois cellulaires
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
PT2314629E (pt) * 2002-07-18 2014-01-22 Merus B V Produção recombinante de misturas de anticorpos
JP2005533861A (ja) * 2002-07-25 2005-11-10 メデュームン，インコーポレーテッド 抗ＲＳＶ、抗ｈＭＰＶ、および抗ＰＩＶ抗体を使用するＲＳＶ、ｈＭＰＶ、およびＰＩＶの治療法と予防法
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
WO2005003326A2 (fr) * 2003-03-28 2005-01-13 Receptors Llc. Recepteurs artificiels comprenant des blocs de construction immobilises de maniere reversible et procedes
US20050136483A1 (en) * 2003-09-03 2005-06-23 Receptors Llc Nanodevices employing combinatorial artificial receptors
US20050037428A1 (en) * 2002-09-16 2005-02-17 Receptors Llc Artificial receptors including reversibly immobilized building blocks, the building blocks, and methods
US7469076B2 (en) * 2003-09-03 2008-12-23 Receptors Llc Sensors employing combinatorial artificial receptors
US20050037429A1 (en) * 2003-03-28 2005-02-17 Receptors Llc Artificial receptors including reversibly immobilized building blocks and methods
US20050170385A1 (en) * 2002-09-16 2005-08-04 Receptors Llc Artificial receptors including gradients
US20040137481A1 (en) * 2002-09-16 2004-07-15 Receptors Llc Artificial receptor building blocks, components, and kits
US20050037381A1 (en) * 2002-09-16 2005-02-17 Receptors Llc Artificial receptors, building blocks, and methods
US20060057625A1 (en) * 2002-09-16 2006-03-16 Carlson Robert E Scaffold-based artificial receptors and methods
CA2502367C (fr) 2002-10-16 2013-12-10 Euro-Celtique S.A. Anticorps se fixant sur des polypeptides ca 125/o772p associes a des cellules et leurs procedes d'utilisation
US7427469B2 (en) * 2002-11-05 2008-09-23 Institut Pasteur Method of treating cytomegalovirus with DC-SIGN blockers
ATE428437T1 (de) * 2002-11-05 2009-05-15 Pasteur Institut Dc-sign blockern und deren verwendung zur vorbeugung oder behandlung von viraleninfektionen
AU2003287622A1 (en) * 2002-11-06 2004-06-03 Fraunhofer Usa Expression of foreign sequences in plants using trans-activation system
US8148608B2 (en) * 2004-02-20 2012-04-03 Fraunhofer Usa, Inc Systems and methods for clonal expression in plants
US7692063B2 (en) * 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7683238B2 (en) * 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US20050079574A1 (en) * 2003-01-16 2005-04-14 Genentech, Inc. Synthetic antibody phage libraries
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
WO2004072266A2 (fr) * 2003-02-13 2004-08-26 Kalobios Inc. Ingenierie de l'affinite des anticorps par un remplacement complementaire en serie guide par epitope
CN1777621A (zh) 2003-03-14 2006-05-24 Wyeth公司 抗人il－21受体的抗体及其应用
BRPI0410562B8 (pt) 2003-05-22 2021-05-25 Fralunhofer Usa Inc proteína de veículo e seu método de produção em uma planta, ácido nucleico isolado, vetor de expressão e célula hospedeira
ES2408582T3 (es) 2003-05-30 2013-06-21 Merus B.V. Biblioteca de Fab para la preparación de una mezcla de anticuerpos
US9708410B2 (en) 2003-05-30 2017-07-18 Janssen Biotech, Inc. Anti-tissue factor antibodies and compositions
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
EP1635763B1 (fr) 2003-06-09 2012-08-08 Alnylam Pharmaceuticals Inc. Procede de traitement de maladie neurodegenerative
WO2005011580A2 (fr) * 2003-07-25 2005-02-10 Board Of Regents, The University Of Texas System Compositions et procedes pour le traitement et la prophylaxie de l'herpes simplex
KR20060069825A (ko) * 2003-08-01 2006-06-22 제넨테크, 인크. 제한된 다양성을 갖는 항체 ｃｄｒ 폴리펩티드 서열
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
US7329725B1 (en) * 2003-10-29 2008-02-12 Nastech Pharmaceutical Company Inc. Phage displayed Trp cage ligands
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
JP4782700B2 (ja) 2004-01-20 2011-09-28 カロバイオス ファーマシューティカルズ インコーポレイティッド 最低限必須な結合決定基を用いた抗体特異性の移入
JP5912211B2 (ja) 2004-01-20 2016-04-27 メルス ビー．ヴィー． 結合タンパク質の混合物
US7785903B2 (en) * 2004-04-09 2010-08-31 Genentech, Inc. Variable domain library and uses
AU2005250369A1 (en) * 2004-05-18 2005-12-15 Genentech, Inc. M13 virus major coat protein variants for C-terminal and BI-terminal display of a heterologous protein
EP2255832A3 (fr) 2004-06-16 2011-02-09 Affinergy, Inc. Biomatériaux d'interface pour favoriser la liaison d'analytes cibles
JP5143551B2 (ja) 2004-07-06 2013-02-13 バイオレン，インク． ユニバーサル抗体のライブラリー
US20060068421A1 (en) * 2004-08-05 2006-03-30 Biosite, Inc. Compositions and methods for phage display of polypeptides
US7504365B2 (en) 2004-09-03 2009-03-17 Receptors Llc Combinatorial artificial receptors including tether building blocks
WO2006029383A2 (fr) * 2004-09-11 2006-03-16 Receptors Llc Recepteurs artificiels combinatoires comprenant des blocs structurels peptidiques
EP1812068A4 (fr) * 2004-10-29 2010-06-09 Medimmune Inc Méthodes de prévention et de traitement des infections à rsv et des états pathologiques associés
EP3623473A1 (fr) 2005-03-31 2020-03-18 Chugai Seiyaku Kabushiki Kaisha Procédé pour la production de polypeptide au moyen de la régulation d'un ensemble
WO2006109592A1 (fr) * 2005-04-08 2006-10-19 Chugai Seiyaku Kabushiki Kaisha Substitution des anticorps de la fonction du facteur viii de coagulation sanguine
LT2460831T (lt) 2005-05-27 2016-12-12 Biogen Ma Inc. Tweak rišantys antikūnai
BRPI0614475B8 (pt) * 2005-08-03 2021-05-25 Fraunhofer Usa Inc polipeptídeo, anticorpo, ácido nucleico, vetores, célula hospedeira, composição e processos de produção de anticorpo
IL172297A (en) 2005-10-03 2016-03-31 Compugen Ltd Soluble vegfr-1 variants for the diagnosis of preeclampsia
US20070122408A1 (en) * 2005-10-20 2007-05-31 The Scripps Research Institute Fc Labeling for Immunostaining and Immunotargeting
EP2465870A1 (fr) 2005-11-07 2012-06-20 Genentech, Inc. Polypeptides de liaison dotés de séquences hypvervariables VH/VL diversifiées et consensuelles
ES2402576T3 (es) * 2005-11-14 2013-05-06 Bioren, Inc. Ultrahumanización de anticuerpos por generación y selección de bibliotecas cohorte y blast de cdr maduras previstas
EP2339014B1 (fr) 2005-11-16 2015-05-27 Ambrx, Inc. Procédés et compositions comprenant des acides aminés non naturels
US20070237764A1 (en) * 2005-12-02 2007-10-11 Genentech, Inc. Binding polypeptides with restricted diversity sequences
KR101538521B1 (ko) 2005-12-15 2015-07-22 제넨테크, 인크. 폴리유비퀴틴 표적화를 위한 방법 및 조성물
DK1973950T3 (en) 2006-01-05 2014-12-15 Genentech Inc ANTI-EphB4 ANTIBODIES AND METHODS FOR USING SAME
US20100184021A1 (en) 2006-01-16 2010-07-22 Compugen Ltd. Novel nucleotide and amino acid sequences, and methods of use thereof for diagnosis
US20070186296A1 (en) * 2006-02-02 2007-08-09 Wyeth Cloning, characterization, and application of tnfrsf19 in neurological disorders
CN101395174B (zh) * 2006-02-13 2014-03-19 艾比欧公司 人类乳头瘤病毒（hpv）抗原、疫苗组合物和相关方法
WO2008048344A2 (fr) * 2006-02-13 2008-04-24 Fraunhofer Usa, Inc. Antigènes de bacillus anthracis, compositions pour vaccins et procédés connexes
JP2009526526A (ja) * 2006-02-13 2009-07-23 フラウンホーファー ユーエスエー， インコーポレイテッド インフルエンザ抗原、ワクチン組成物、および関連する方法
AR059851A1 (es) 2006-03-16 2008-04-30 Genentech Inc Anticuerpos de la egfl7 y metodos de uso
WO2007109243A2 (fr) * 2006-03-20 2007-09-27 Duramed Pharmaceuticals, Inc. Procedes de comparaison de l'immunogenicite de produits et leurs utilisations
JP5624276B2 (ja) 2006-03-31 2014-11-12 中外製薬株式会社 抗体の血中動態を制御する方法
US9670269B2 (en) 2006-03-31 2017-06-06 Chugai Seiyaku Kabushiki Kaisha Methods of modifying antibodies for purification of bispecific antibodies
CA2651567A1 (fr) * 2006-05-09 2007-11-22 Genentech, Inc. Polypeptides de liaison a squelettes optimises
EP2024393A2 (fr) * 2006-05-15 2009-02-18 Sea Lane Biotechnologies,llc. Anticorps neutralisants dirigés contre les virus de la grippe
US8148085B2 (en) 2006-05-15 2012-04-03 Sea Lane Biotechnologies, Llc Donor specific antibody libraries
MX2008015132A (es) 2006-05-30 2008-12-10 Genentech Inc Anticuerpos e inmunoconjugados y sus usos.
KR20090027227A (ko) 2006-06-06 2009-03-16 제넨테크, 인크. 항-ｄｌｌ4 항체 및 이의 사용 방법
US20100003189A1 (en) 2006-07-14 2010-01-07 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
US20090252745A1 (en) * 2006-09-15 2009-10-08 Duke University Amino acids in the HCV core polypeptide domain 3 and correlation with steatosis
JP5473603B2 (ja) 2006-10-02 2014-04-16 シー レーン バイオテクノロジーズ， エルエルシー 多様な合成ペプチドおよびポリペプチドライブラリーの設計および構築
EP2061814B1 (fr) 2006-10-27 2012-06-06 Genentech, Inc. Anticorps et immunoconjugues, et leurs utilisations
EP2115173B1 (fr) 2007-01-08 2011-09-21 Government of the USA, as Represented by the Secretary, Department of Health and Human Services Genotype slco1b3
US9896511B2 (en) 2007-01-10 2018-02-20 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Antibodies that bind to TL1A and methods of treating inflammatory or autoimmune disease comprising administering such antibodies
US9068003B2 (en) 2007-01-10 2015-06-30 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Antibodies that bind to TL1A and methods of treating inflammatory or autoimmune disease comprising administering such antibodies
US7960139B2 (en) 2007-03-23 2011-06-14 Academia Sinica Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells
BRPI0810865A2 (pt) * 2007-04-28 2017-05-09 Fraunhofer Usa Inc antígenos de tripanossoma, composições vacinais, e métodos relacionados
EP1997830A1 (fr) 2007-06-01 2008-12-03 AIMM Therapeutics B.V. Molécules à liaison spécifiques RSV et leur moyen de fabrication
WO2009009759A2 (fr) 2007-07-11 2009-01-15 Fraunhofer Usa, Inc. Antigènes yersinia pestis, compositions de vaccins, et méthodes associées
WO2009012256A1 (fr) 2007-07-16 2009-01-22 Genentech, Inc. Anticorps anti-cd79b humanisés, immunoconjugués, et procédés d'utilisation
DK2474557T3 (da) 2007-07-16 2014-11-10 Genentech Inc Anti-CD79b-antistoffer og immunkonjugater og fremgangsmåder til anvendelse
EP2190472A2 (fr) * 2007-08-20 2010-06-02 Fraunhofer USA, Inc. Vaccins antigrippaux prophylactiques et thérapeutiques, antigènes, compositions et procédés
US20100239596A1 (en) * 2007-08-22 2010-09-23 University Of Southern California Grp78 and tumor angiogenesis
US8691730B2 (en) 2007-09-14 2014-04-08 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP3689912A1 (fr) 2007-09-26 2020-08-05 Chugai Seiyaku Kabushiki Kaisha Procédé de modification d'un anticorps par point isoélectrique par substitution d'acide aminé dans cdr
EP2230300A4 (fr) 2007-10-24 2012-08-08 Otsuka Chemical Holdings Co Ltd Polypeptide à fonction effectrice améliorée
CA2705152C (fr) 2007-11-09 2016-10-11 Peregrine Pharmaceuticals, Inc. Compositions d'anticorps anti-vegf et procedes correspondants
AR069501A1 (es) 2007-11-30 2010-01-27 Genentech Inc Anticuerpos anti- vegf (factor de crecimiento endotelial vascular)
CN101977934B (zh) 2008-01-18 2014-09-17 健泰科生物技术公司 用于靶向k63连接的多聚遍在蛋白的方法和组合物
DK2657253T3 (en) 2008-01-31 2017-10-09 Genentech Inc Anti-CD79b antibodies and immune conjugates and methods of use
TWI564021B (zh) 2008-04-11 2017-01-01 Chugai Pharmaceutical Co Ltd Repeated binding of antigen to antigen binding molecules
EP3388527A1 (fr) * 2008-05-15 2018-10-17 Tetherex Pharmaceuticals Corporation Anticorps anti-psgl-1 et procédés d'identification et d'utilisation
US20090291073A1 (en) * 2008-05-20 2009-11-26 Ward Keith W Compositions Comprising PKC-theta and Methods for Treating or Controlling Ophthalmic Disorders Using Same
JP2009278908A (ja) * 2008-05-22 2009-12-03 Fujifilm Corp 抗オステオカルシン抗体及びそれを用いた免疫測定方法
KR20110031343A (ko) 2008-06-20 2011-03-25 와이어쓰 엘엘씨 베타-용혈성 스트렙토코커스 균주로부터 유래된 ｏｒｆ1358을 사용하는 조성물 및 방법
EP2318832B1 (fr) 2008-07-15 2013-10-09 Academia Sinica Matrices de glycane sur des lames de verre revêtues d' aluminium de type ptfe et procédés associés
AU2009274129B2 (en) * 2008-07-21 2016-02-25 Immunomedics Inc. Structural variants of antibodies for improved therapeutic characteristics
KR20160116056A (ko) 2008-08-14 2016-10-06 테바 파마슈티컬즈 오스트레일리아 피티와이 엘티디 항-ｉｌ-12/ｉｌ-23 항체
US8946291B2 (en) 2008-08-15 2015-02-03 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Compositions and methods for treating pigmentary conditions and melanoma
US20100081575A1 (en) * 2008-09-22 2010-04-01 Robert Anthony Williamson Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules
AU2009293640A1 (en) * 2008-09-22 2010-03-25 Calmune Corporation Methods and vectors for display of 2G12 -derived domain exchanged antibodies
US8734803B2 (en) 2008-09-28 2014-05-27 Ibio Inc. Humanized neuraminidase antibody and methods of use thereof
WO2010039801A2 (fr) 2008-10-02 2010-04-08 The J. David Gladstone Institutes Méthodes de traitement d’une infection par le virus de l’hépatite c
CA2744555A1 (fr) 2008-11-26 2010-06-03 Five Prime Therapeutics, Inc. Compositions et procedes pour reguler l'expression de collagene et d'actine de muscle lisse par la serpine e2
WO2010061393A1 (fr) 2008-11-30 2010-06-03 Compugen Ltd. Séquences d'acides aminés et de nucléotides de variants de he4 et leurs procédés d'utilisation
CN101768213B (zh) 2008-12-30 2012-05-30 中国科学院遗传与发育生物学研究所 一种与植物分蘖数目相关的蛋白及其编码基因与应用
CN101817879A (zh) 2009-02-26 2010-09-01 中国科学院遗传与发育生物学研究所 金属硫蛋白及其编码基因与应用
SI3702371T1 (sl) 2009-03-25 2023-01-31 Genentech, Inc. Protitelesa proti FGFR3 in postopki za uporabo le-teh
US8466260B2 (en) 2009-04-01 2013-06-18 Genentech, Inc. Anti-FcRH5 antibodies and immunoconjugates and methods of use
WO2010129033A2 (fr) * 2009-04-29 2010-11-11 Calmune Corporation Anticorps modifiés pour immunothérapie passive
CN102459591B (zh) * 2009-05-20 2015-05-13 诺维莫尼公司 合成性的多肽文库以及用于建立具有天然多样性的多肽变体的方法
MX2011012696A (es) 2009-05-29 2012-03-29 Morphosys Ag Una coleccion y metodos para su uso.
WO2011014457A1 (fr) 2009-07-27 2011-02-03 Genentech, Inc. Traitements d’association
CA2770737C (fr) * 2009-08-13 2020-05-12 Crucell Holland B.V. Anticorps contre le virus respiratoire syncytial (vrs) humain et procedes d'utilisation
EP2473522B1 (fr) 2009-09-02 2016-08-17 Genentech, Inc. Mutant smoothened et ses méthodes d'utilisation
WO2011035205A2 (fr) 2009-09-18 2011-03-24 Calmune Corporation Anticorps dirigés contre candida, leurs collectes et procédés d'utilisation
EP2483307A1 (fr) 2009-09-29 2012-08-08 Fraunhofer USA, Inc. Anticorps dirigés contre l'hémagglutinine du virus de la grippe, compositions, et procédés associés
US8568726B2 (en) 2009-10-06 2013-10-29 Medimmune Limited RSV specific binding molecule
EP2491059B1 (fr) 2009-10-22 2015-02-25 F.Hoffmann-La Roche Ag Anticorps anti-hepsine et procédés d'utilisation de ceux-ci
WO2011056502A1 (fr) 2009-10-26 2011-05-12 Genentech, Inc. Compositions du gene recepteur de proteine morphogenetique osseuse type ii et procedes d'utilisation
WO2011056494A1 (fr) 2009-10-26 2011-05-12 Genentech, Inc. Combinaisons d'antagonistes de la kinase-1 du type récepteur de l'activine et d'antagonistes vegfr3
WO2011056497A1 (fr) 2009-10-26 2011-05-12 Genentech, Inc. Compositions de récepteur de l'activine de type iib et leurs méthodes d'utilisation
US20120316071A1 (en) 2009-11-04 2012-12-13 Vaughn Smider Methods for affinity maturation-based antibody optimization
BR112012010153B1 (pt) 2009-11-05 2022-05-03 Genentech, Inc Método de produção de um anticorpo
US11377485B2 (en) 2009-12-02 2022-07-05 Academia Sinica Methods for modifying human antibodies by glycan engineering
US10087236B2 (en) 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
US8486397B2 (en) 2009-12-11 2013-07-16 Genentech, Inc. Anti-VEGF-C antibodies and methods using same
RU2609658C2 (ru) 2009-12-21 2017-02-02 Дженентек, Инк. Состав, содержащий антитело
CA2784385A1 (fr) 2009-12-23 2011-06-30 Genentech, Inc. Anticorps anti-bv8 et leurs utilisations
US8685524B2 (en) 2010-01-29 2014-04-01 Toray Industries, Inc. Polylactic acid-based resin sheet
PT2505654T (pt) 2010-02-08 2016-11-18 Regeneron Pharma Rato de cadeia leve comum
US9796788B2 (en) 2010-02-08 2017-10-24 Regeneron Pharmaceuticals, Inc. Mice expressing a limited immunoglobulin light chain repertoire
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
US10338069B2 (en) 2010-04-12 2019-07-02 Academia Sinica Glycan arrays for high throughput screening of viruses
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
KR101745386B1 (ko) 2010-05-10 2017-06-09 아카데미아 시니카 항-인플루엔자 활성을 가진 자나미비르 포스포네이트 동족체 및 인플루엔자 바이러스의 오셀타미비르 감수성을 확인하는 방법
MX336109B (es) 2010-06-03 2016-01-08 Genentech Inc Formacion de imagenes inmuno-tep de anticuerpos e inmunoconjugados y usos de lo mismo.
NZ703035A (en) 2010-07-09 2016-06-24 Crucell Holland Bv Anti-human respiratory syncytial virus (rsv) antibodies and methods of use
WO2012019061A2 (fr) 2010-08-05 2012-02-09 Stem Centrx, Inc. Nouveaux effecteurs et leurs procédés d'utilisation
SG10201506782XA (en) 2010-08-27 2015-10-29 Stem Centrx Inc Notum protein modulators and methods of use
CN106620693A (zh) 2010-09-03 2017-05-10 艾伯维施特姆森特克斯有限责任公司 新型调节剂及使用方法
MX349622B (es) 2010-09-08 2017-08-07 Halozyme Inc Metodos para evaluar e identificar o evolucionar proteinas terapeuticas condicionalmente activas.
DK2625197T3 (en) 2010-10-05 2016-10-03 Genentech Inc Smoothened MUTANT AND METHODS OF USING THE SAME
JP6243737B2 (ja) 2010-10-27 2017-12-06 ザ リサーチ ファウンデーション フォー ザ ステイト ユニバーシティ オブ ニューヨーク Ｅ−カドヘリンの可溶性細胞外ドメインを標的とする組成物および癌治療のための関連方法
MX355060B (es) 2010-11-17 2018-04-03 Chugai Pharmaceutical Co Ltd Molecula multiespecifica de union a antigeno que tiene funcion alternativa a la funcion del factor viii de coagulacion sanguinea.
SG189950A1 (en) 2010-11-19 2013-06-28 Morphosys Ag A collection and methods for its use
RU2592672C9 (ru) 2010-12-08 2016-11-27 СтемСентРкс, Инк. Новые модуляторы и способы их применения
WO2012092539A2 (fr) 2010-12-31 2012-07-05 Takeda Pharmaceutical Company Limited Anticorps contre dll4 et leurs utilisations
SA112330278B1 (ar) 2011-02-18 2015-10-09 ستيم سينتركس، انك. مواد ضابطة جديدة وطرق للاستخدام
AU2012245073B2 (en) 2011-04-21 2016-02-11 Garvan Institute Of Medical Research Modified variable domain molecules and methods for producing and using them b
EP3865581A1 (fr) 2011-08-05 2021-08-18 Regeneron Pharmaceuticals, Inc. Souris à chaîne légère universelle humanisée
US20130058947A1 (en) 2011-09-02 2013-03-07 Stem Centrx, Inc Novel Modulators and Methods of Use
EP2761006B1 (fr) 2011-09-28 2016-12-14 Zera Intein Protein Solutions, S.L. Intéines divisées et leurs utilisations
JP5936145B2 (ja) * 2011-09-29 2016-06-15 Ｎｅｃソリューションイノベータ株式会社 ペプチド抗体のスクリーニングに使用する核酸構築物およびそれを用いたスクリーニング方法
US20150050269A1 (en) 2011-09-30 2015-02-19 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities
US11851476B2 (en) 2011-10-31 2023-12-26 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule having regulated conjugation between heavy-chain and light-chain
JP2015509091A (ja) 2012-01-09 2015-03-26 ザ スクリプス リサーチ インスティテュート ヒト化抗体
WO2013106485A2 (fr) 2012-01-09 2013-07-18 The Scripps Research Institute Régions déterminant la complémentarité ultralongues et utilisations associées
WO2013126746A2 (fr) 2012-02-24 2013-08-29 Stem Centrx, Inc. Nouveaux modulateurs et leurs procédés d'utilisation
AU2013229786B2 (en) 2012-03-08 2017-06-22 Halozyme, Inc. Conditionally active anti-epidermal growth factor receptor antibodies and methods of use thereof
NZ730271A (en) 2012-03-16 2022-09-30 Regeneron Pharma Non-human animals expressing ph-sensitive immunoglobulin sequences
US20140013456A1 (en) 2012-03-16 2014-01-09 Regeneron Pharmaceuticals, Inc. Histidine Engineered Light Chain Antibodies and Genetically Modified Non-Human Animals for Generating the Same
LT2883449T (lt) 2012-03-16 2018-05-10 Regeneron Pharmaceuticals, Inc. Histidinu modifikuoti lengvosios grandinės antikūnai ir juos gaminantys genetiškai modifikuoti graužikai
WO2013138522A2 (fr) 2012-03-16 2013-09-19 Genelux Corporation Méthodes d'évaluation de l'efficacité et de la surveillance d'un traitement viral oncolytique
WO2013138681A1 (fr) 2012-03-16 2013-09-19 Regeneron Pharmaceuticals, Inc. Souris produisant des protéines de liaison à l'antigène ayant des caractéristiques de liaison dépendant du ph
US10130714B2 (en) 2012-04-14 2018-11-20 Academia Sinica Enhanced anti-influenza agents conjugated with anti-inflammatory activity
NZ772318A (en) 2012-04-20 2023-06-30 Merus Nv Methods and means for the production of ig-like molecules
EP2692865B1 (fr) * 2012-07-30 2014-12-10 NBE-Therapeutics LLC Identification induite par transposition de liaison spécifique ou protéines fonctionnelles
US9914956B2 (en) 2012-08-18 2018-03-13 Academia Sinica Cell-permeable probes for identification and imaging of sialidases
WO2014031762A1 (fr) 2012-08-21 2014-02-27 Academia Sinica Composés benzocyclo-octyne et leurs utilisations
AU2013359419B2 (en) 2012-12-10 2018-03-15 Biogen Ma Inc. Anti-blood dendritic cell antigen 2 antibodies and uses thereof
US10717965B2 (en) 2013-01-10 2020-07-21 Gloriana Therapeutics, Inc. Mammalian cell culture-produced neublastin antibodies
CN105164159A (zh) 2013-02-22 2015-12-16 施特姆森特克斯股份有限公司 新的抗体缀合物及其用途
CA2904169C (fr) 2013-03-13 2021-12-07 Genentech, Inc. Formulations avec oxydation reduite
MX369671B (es) 2013-03-13 2019-11-15 Genentech Inc Formulaciones con oxidacion reducida.
US20140314778A1 (en) 2013-03-13 2014-10-23 Genentech, Inc. Formulations with reduced oxidation
US10653779B2 (en) 2013-03-13 2020-05-19 Genentech, Inc. Formulations with reduced oxidation
BR112015022210A8 (pt) 2013-03-13 2018-01-23 Genentech Inc formulações de anticorpo
EP2968544A4 (fr) 2013-03-15 2016-10-12 Hoffmann La Roche Support de culture cellulaire et procédés de production d'anticorps
ES2798307T3 (es) 2013-03-15 2020-12-10 Hoffmann La Roche Composiciones de cultivo de células con antioxidantes y procedimientos para la producción de polipéptidos
CN105392801A (zh) 2013-03-15 2016-03-09 比奥根Ma公司 使用抗αvβ5抗体治疗和预防急性肾损伤
EP3013365B1 (fr) 2013-06-26 2019-06-05 Academia Sinica Antigènes rm2 et leur utilisation
WO2014210564A1 (fr) 2013-06-27 2014-12-31 Academia Sinica Conjugués de glycane et leur utilisation
WO2015017146A2 (fr) 2013-07-18 2015-02-05 Fabrus, Inc. Anticorps à régions de détermination de complémentarité ultralongues
CN111518199A (zh) 2013-07-18 2020-08-11 图鲁斯生物科学有限责任公司 具有超长互补决定区的人源化抗体
EP3916081A3 (fr) 2013-08-19 2022-03-23 Biogen MA Inc. Régulation de la glycosylation des protéines par supplémentation du milieu de culture et par les paramètres du procédé de culture cellulaire
KR20160046914A (ko) 2013-08-28 2016-04-29 스템센트알엑스 인코포레이티드 신규한 ｓｅｚ6 조절물질 및 사용방법
CN105848671B (zh) 2013-08-28 2019-12-13 艾伯维施特姆森特克斯有限责任公司 位点特异性抗体缀合方法和组合物
US9782476B2 (en) 2013-09-06 2017-10-10 Academia Sinica Human iNKT cell activation using glycolipids with altered glycosyl groups
MX2016003256A (es) 2013-09-12 2016-06-07 Halozyme Inc Anticuerpos modificados del receptor de factor de crecimiento anti-epidermico y metodos de uso de los mismos.
HUE057005T2 (hu) 2013-09-25 2022-04-28 Bioverativ Therapeutics Inc Oszlopon történõ vírusinaktiváló eljárások
DK3050896T3 (da) 2013-09-27 2021-07-19 Chugai Pharmaceutical Co Ltd Fremgangsmåde til fremstilling af en polypeptid-heteromultimer
WO2015048520A1 (fr) 2013-09-27 2015-04-02 Genentech, Inc. Formulations d'anticorps anti-pdl1
WO2015071759A1 (fr) 2013-11-15 2015-05-21 Institut Pasteur Marqueur moléculaire de la résistance de plasmodium falciparum à l'artémisinine
WO2015095809A1 (fr) 2013-12-20 2015-06-25 Biogen Idec Ma Inc. Utilisation de cultures de semences sous perfusion pour améliorer la capacité de production en alimentation programmée de produits biopharmaceutiques et la qualité des produits
EP2960252A1 (fr) 2014-06-26 2015-12-30 Institut Pasteur Phospholipase pour le Traitment d'immunosuppression
HRP20220748T1 (hr) 2013-12-24 2022-09-02 Janssen Pharmaceutica Nv Anti-vista antitijela i fragmenti
WO2015103438A2 (fr) 2014-01-02 2015-07-09 Genelux Corporation Traitement d'appoint par un virus oncolytique avec des agents qui augmentent l'infectivité du virus
US10150818B2 (en) 2014-01-16 2018-12-11 Academia Sinica Compositions and methods for treatment and detection of cancers
JP2017507118A (ja) 2014-01-16 2017-03-16 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ がんの処置および検出のための組成物および方法
WO2016114819A1 (fr) 2015-01-16 2016-07-21 Academia Sinica Compositions et méthodes pour traiter et détecter des cancers
WO2015120075A2 (fr) 2014-02-04 2015-08-13 Genentech, Inc. Mutant smoothened et méthodes d'utilisation de celui-ci
MX2016010677A (es) 2014-02-21 2017-04-10 Abbvie Stemcentrx Llc Conjugados de anticuerpos anti-drosophila similar a delta 3 (anti-dll3) y medicamentos para usarse en el tratamiento contra melanoma.
EP3119194B1 (fr) 2014-03-21 2021-04-28 Regeneron Pharmaceuticals, Inc. Animaux non humains qui produisent des protéines de liaison monodomaine
US11124760B2 (en) 2014-03-24 2021-09-21 Biogen Ma Inc. Methods for overcoming glutamine deprivation during mammalian cell culture
TWI687428B (zh) 2014-03-27 2020-03-11 中央研究院 反應性標記化合物及其用途
EP4050026A1 (fr) 2014-04-01 2022-08-31 Adimab, LLC Procédé d'obtention ou d'identification d'une ou plusieurs chaînes légères communes à utiliser dans la préparation d'un anticorps multispécifique
ES2851451T3 (es) 2014-05-13 2021-09-07 Bavarian Nordic As Terapia de combinación para tratar cáncer con un poxvirus que expresa un antígeno de tumor y un anticuerpo monoclonal contra TIM-3
KR102512592B1 (ko) 2014-05-27 2023-03-21 아카데미아 시니카 항-ｈｅｒ2 글리코항체 및 이의 용도
CA2950415A1 (fr) 2014-05-27 2015-12-03 Academia Sinica Glycoanticorps anti-cd20 et leurs utilisations
US10118969B2 (en) 2014-05-27 2018-11-06 Academia Sinica Compositions and methods relating to universal glycoforms for enhanced antibody efficacy
EP3149045B1 (fr) 2014-05-27 2023-01-18 Academia Sinica Compositions et procédés concernant des glycoformes universelles pour une efficacité d'anticorps améliorée
KR102494193B1 (ko) 2014-05-28 2023-01-31 아카데미아 시니카 항-ｔｎｆ-알파 글리코항체 및 이의 용도
TWI695011B (zh) 2014-06-18 2020-06-01 美商梅爾莎納醫療公司 抗ｈｅｒ２表位之單株抗體及其使用之方法
HUE043847T2 (hu) 2014-08-28 2019-09-30 Halozyme Inc Hialuronán-lebontó enzimmel és egy immun checkpoint inhibitorral végzett kombinációs terápia
KR102422375B1 (ko) 2014-09-08 2022-07-18 아카데미아 시니카 당지질을 사용한 인간 iNKT 세포 활성화
EP3193932B1 (fr) 2014-09-15 2023-04-26 F. Hoffmann-La Roche AG Formulations d'anticorps
TWI700300B (zh) 2014-09-26 2020-08-01 日商中外製藥股份有限公司 中和具有第ｖｉｉｉ凝血因子（ｆｖｉｉｉ）機能替代活性的物質之抗體
MA40764A (fr) 2014-09-26 2017-08-01 Chugai Pharmaceutical Co Ltd Agent thérapeutique induisant une cytotoxicité
TWI701435B (zh) 2014-09-26 2020-08-11 日商中外製藥股份有限公司 測定ｆｖｉｉｉ的反應性之方法
MA41685A (fr) 2014-10-17 2017-08-22 Biogen Ma Inc Supplémentation en cuivre pour la régulation de la glycosylation dans un procédé de culture cellulaire de mammifère
MA40864A (fr) 2014-10-31 2017-09-05 Biogen Ma Inc Hypotaurine, gaba, bêta-alanine et choline pour la régulation de l'accumulation de sous-produits résiduaires dans des procédés de culture de cellules mammifères
MX2017003478A (es) 2014-11-05 2018-02-01 Genentech Inc Anticuerpos anti-fgfr2/3 y metodos para su uso.
ES2832802T3 (es) 2014-11-21 2021-06-11 Univ Maryland Sistemas de administración dirigida del particulado específico de una estructura
EP3227687A4 (fr) 2014-12-05 2018-10-24 Prelude, Inc. Récurrence de carcinome canalaire in situ (dcis) et cancer du sein invasif
US9975965B2 (en) 2015-01-16 2018-05-22 Academia Sinica Compositions and methods for treatment and detection of cancers
US10495645B2 (en) 2015-01-16 2019-12-03 Academia Sinica Cancer markers and methods of use thereof
EP3248013B1 (fr) 2015-01-24 2020-07-15 Academia Sinica Marqueurs de cancer et leurs procédés d'utilisation
EP3248005B1 (fr) 2015-01-24 2020-12-09 Academia Sinica Nouveaux composés conjugués de glycane et leurs méthodes d'utilisation
CN107614521B (zh) 2015-01-30 2022-02-08 台湾地区“中央研究院” 增进抗体功效的通用糖型组合物及方法
JP2018507254A (ja) 2015-02-02 2018-03-15 アイツー ファーマシューティカルズ， インコーポレーテッド 抗代替軽鎖抗体
CA2975875A1 (fr) 2015-02-04 2016-08-11 Genentech, Inc. Mutant smoothened et methodes d'utilisation de celui-ci
US11111314B2 (en) 2015-03-19 2021-09-07 Regeneron Pharmaceuticals, Inc. Non-human animals that select for light chain variable regions that bind antigen
WO2016159213A1 (fr) 2015-04-01 2016-10-06 中外製薬株式会社 Procédé pour la production d'un hétéro-oligomère polypeptidique
WO2016164708A1 (fr) 2015-04-10 2016-10-13 Adimab, Llc Procédés de purification d'anticorps hétérodimères multispécifiques mélangés à des espèces d'anticorps homodimères parentaux
EP3285811A1 (fr) 2015-04-21 2018-02-28 Institut Gustave Roussy Procédés thérapeutiques, produits et compositions inhibant znf555
DK3313882T3 (da) 2015-06-24 2020-05-11 Janssen Pharmaceutica Nv Anti-VISTA antistoffer og fragmenter
TW202340452A (zh) 2015-08-04 2023-10-16 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
JP2018532990A (ja) 2015-09-04 2018-11-08 オービーアイ ファーマ，インコーポレイテッド グリカンアレイおよび使用の方法
JP7219005B2 (ja) 2015-12-28 2023-02-07 中外製薬株式会社 Ｆｃ領域含有ポリペプチドの精製を効率化するための方法
SG11201804961UA (en) 2015-12-30 2018-07-30 Genentech Inc Formulations with reduced degradation of polysorbate
BR112018013071A2 (pt) 2015-12-30 2018-12-11 Genentech Inc uso de derivados de triptofano para formulações de proteínas
CA3019952A1 (fr) 2016-02-04 2017-08-10 Curis, Inc. Mutant smoothened et methodes d'utilisation dudit mutant
US10899836B2 (en) 2016-02-12 2021-01-26 Janssen Pharmaceutica Nv Method of identifying anti-VISTA antibodies
JP2019515876A (ja) 2016-03-08 2019-06-13 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ Ｎ−グリカンおよびそのアレイのモジュール合成のための方法
EP4302782A3 (fr) 2016-03-15 2024-03-27 Mersana Therapeutics, Inc. Conjugués anticorps-médicament ciblant napi2b et leurs procédés d'utilisation
WO2017161206A1 (fr) 2016-03-16 2017-09-21 Halozyme, Inc. Conjugués contenant des anticorps à activité conditionnelle ou des fragments de liaison à un antigène associés, et procédés d'utilisation
WO2017165665A1 (fr) 2016-03-23 2017-09-28 The General Hospital Corporation Dosages et procédés pour détecter l'udp-glucose
CA3019560A1 (fr) 2016-03-29 2017-10-05 Obi Pharma, Inc. Anticorps, compositions pharmaceutiques et procedes
US10765724B2 (en) 2016-03-29 2020-09-08 Janssen Biotech, Inc. Method of treating psoriasis with increased interval dosing of anti-IL12/23 antibody
US10980894B2 (en) 2016-03-29 2021-04-20 Obi Pharma, Inc. Antibodies, pharmaceutical compositions and methods
WO2017175176A1 (fr) * 2016-04-06 2017-10-12 Zumutor Biologics, Inc. Vecteurs utilisés pour le clonage et l'expression de protéines, leurs procédés et leurs applications
WO2017175058A1 (fr) 2016-04-07 2017-10-12 Janssen Pharmaceutica Nv Anticorps anti-vista et fragments de ceux-ci, leurs utilisations et leurs procédés d'identification
EP3445395A4 (fr) 2016-04-22 2019-12-25 OBI Pharma, Inc. Immunothérapie anticancéreuse par immuno-activation ou immunomodulation par l'intermédiaire d'antigènes de la série globo
US20190330318A1 (en) 2016-07-25 2019-10-31 Biogen Ma Inc. Anti-hspa5 (grp78) antibodies and uses thereof
KR20230117482A (ko) 2016-07-27 2023-08-08 오비아이 파머 인코퍼레이티드 면역원성/치료 글리칸 조성물 및 그의 용도
EP3491026A4 (fr) 2016-07-29 2020-07-29 OBI Pharma, Inc. Anticorps humains, compositions pharmaceutiques et procédés
CN109963868B (zh) 2016-08-22 2023-11-14 醣基生医股份有限公司 抗体、结合片段及使用方法
CN109661241A (zh) 2016-09-06 2019-04-19 中外制药株式会社 使用识别凝血因子ix和/或活化凝血因子ix以及凝血因子x和/或活化凝血因子x的双特异性抗体的方法
CA3037961A1 (fr) 2016-09-30 2018-04-05 Janssen Biotech, Inc. Procede sur et efficace de traitement du psoriasis avec un anticorps specifique contre l'il-23
JP2020502261A (ja) 2016-11-16 2020-01-23 ヤンセン バイオテツク，インコーポレーテツド 抗ｉｌ２３特異的抗体で乾癬を治療する方法
BR112019010356A2 (pt) 2016-11-21 2019-08-27 Obi Pharma Inc molécula de carboidrato, conjugado fármaco-anticorpo (adc), composição, método para preparar a composição, método para tratar câncer em um indivíduo, método para induzir ou melhorar a reação imune em um indivíduo necessitando do mesmo, composto conjugado de fármaco-anticorpo, uso de um composto conjungado anticorpo-fáramco, método para determinar a eficácia terapêutica de células de câncer ao adcc e método para fazer imagem de um indivíduo
KR20240038146A (ko) 2017-01-30 2024-03-22 얀센 바이오테크 인코포레이티드 활성 건선성 관절염의 치료를 위한 항-tnf 항체, 조성물, 및 방법
CN110418652A (zh) 2017-02-07 2019-11-05 詹森生物科技公司 用于治疗活动性强直性脊柱炎的抗tnf抗体、组合物和方法
US12025615B2 (en) 2017-09-15 2024-07-02 Arizona Board Of Regents On Behalf Of Arizona State University Methods of classifying response to immunotherapy for cancer
TW201922780A (zh) 2017-09-25 2019-06-16 美商健生生物科技公司 以抗ｉｌ１２／ｉｌ２３抗體治療狼瘡之安全且有效之方法
MX2020002710A (es) 2017-09-29 2020-07-20 Chugai Pharmaceutical Co Ltd Molecula de union al antigeno multiespecifica que tiene actividad de sustitucion de la funcion de cofactor del factor viii de coagulacion de sangre (fviii) y formulacion farmaceutica que contiene tal molecula como ingrediente activo.
WO2019150309A1 (fr) 2018-02-02 2019-08-08 Hammack Scott Modulateurs de gpr68 et leurs utilisations pour le traitement et la prévention de maladies
EP3530282A1 (fr) 2018-02-27 2019-08-28 Diaccurate Procédés thérapeutiques
CA3092551A1 (fr) 2018-03-05 2019-09-12 Janssen Biotech, Inc. Methodes de traitement de la maladie de crohn avec un anticorps specifique anti-il23
BR112020018235A2 (pt) * 2018-03-14 2020-12-29 F. Hoffmann-La Roche Ag Métodos para gerar uma biblioteca de polinucleotídeos, bibliotecas de polinucleotídeos, uso de uma biblioteca, método para gerar uma biblioteca de anticorpos e método de seleção de um anticorpo
US11203645B2 (en) 2018-06-27 2021-12-21 Obi Pharma, Inc. Glycosynthase variants for glycoprotein engineering and methods of use
WO2020016838A2 (fr) 2018-07-18 2020-01-23 Janssen Biotech, Inc. Prédicteurs de réponse prolongée après le traitement avec un anticorps spécifique anti-il23
WO2020033485A1 (fr) 2018-08-08 2020-02-13 Genentech, Inc. Utilisation de dérivés du tryptophane pour de l-méthionine pour une formulation protéique
WO2020056338A1 (fr) 2018-09-14 2020-03-19 Prelude Corporation Méthode de sélection pour le traitement d'individus présentant un risque de cancer du sein invasif
DK3883606T5 (da) 2018-09-24 2024-01-02 Janssen Biotech Inc Sikker og effektiv fremgangsmåde til behandling af colitis ulcerosa med anti-il12/il23-antistof
JP2022512595A (ja) 2018-10-05 2022-02-07 バヴァリアン・ノルディック・アクティーゼルスカブ 組み換えｍｖａ、及び免疫チェックポイントアンタゴニストまたは免疫チェックポイントアゴニストの静脈内投与によって、がんを治療する併用療法
WO2020086408A1 (fr) 2018-10-26 2020-04-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Bioprocédé d'expression génique transitoire basé sur une perfusion à haut rendement
KR20210093973A (ko) 2018-11-20 2021-07-28 얀센 바이오테크 인코포레이티드 항-il23 특이적 항체로 건선을 치료하는 안전하고 효과적인 방법
EP3883599A1 (fr) 2018-11-20 2021-09-29 Bavarian Nordic A/S Thérapie pour le traitement du cancer par une administration intratumorale et/ou intraveineuse d'un mva recombinant codant pour 4-1bbl (cd137l) et/ou cd40l
WO2020128864A1 (fr) 2018-12-18 2020-06-25 Janssen Biotech, Inc. Méthode sûre et efficace de traitement du lupus avec un anticorps anti-il12/il23
MX2021008537A (es) 2019-01-15 2021-11-12 Janssen Biotech Inc Composiciones de anticuerpos anti-tnf y métodos para el tratamiento de la artritis idiopática juvenil.
KR20210118878A (ko) 2019-01-23 2021-10-01 얀센 바이오테크 인코포레이티드 건선성 관절염의 치료 방법에 사용하기 위한 항-tnf 항체 조성물
EP3693063A1 (fr) 2019-02-06 2020-08-12 Diaccurate Procédés et compositions de traitement du cancer
EP3938384A4 (fr) 2019-03-14 2022-12-28 Janssen Biotech, Inc. Méthodes de fabrication permettant de produire des compositions d'anticorps anti-il12/il23
JP2022526881A (ja) 2019-03-14 2022-05-27 ヤンセン バイオテツク，インコーポレーテツド 抗ｔｎｆ抗体組成物を産生するための方法
US20220153829A1 (en) 2019-03-14 2022-05-19 Janssen Biotech, Inc. Methods for Producing Anti-TNF Antibody Compositions
MA55282A (fr) 2019-03-14 2022-01-19 Janssen Biotech Inc Procédés de fabrication pour la production de compositions d'anticorps anti-tnf
BR112021018441A2 (pt) 2019-03-18 2023-02-28 Janssen Biotech Inc Método para tratamento de psoríase em indivíduos pediátricos com anticorpo anti-il12/il23
AU2020257748A1 (en) 2019-04-19 2021-11-18 Chugai Seiyaku Kabushiki Kaisha Chimeric receptor recognizing modification site of antibody
MX2021014302A (es) 2019-05-23 2022-01-04 Janssen Biotech Inc Metodo para tratar la enfermedad inflamatoria del intestino con una terapia de combinacion de anticuerpos contra il-23 y tnf alfa.
WO2020245676A1 (fr) 2019-06-03 2020-12-10 Janssen Biotech, Inc. Compositions d'anticorps anti-tnf et méthodes pour le traitement de l'arthrite psoriasique
CA3142580A1 (fr) 2019-06-03 2020-12-10 Janssen Biotech, Inc. Anticorps anti-tnf, compositions et methodes pour le traitement de la spondylarthrite ankylosante active
JPWO2021010326A1 (fr) 2019-07-12 2021-01-21
WO2021028752A1 (fr) 2019-08-15 2021-02-18 Janssen Biotech, Inc. Anticorps anti-tfn pour le traitement du diabète de type i
EP4038222A4 (fr) 2019-10-02 2023-10-18 Arizona Board of Regents on behalf of Arizona State University Procédés et compositions pour identifier des néo-antigènes destinés à être utilisés dans le traitement et la prévention du cancer
EP4061406A1 (fr) 2019-11-20 2022-09-28 Bavarian Nordic A/S Virus mva recombinants pour l'administration intratumorale et/ou intraveineuse pour le traitement du cancer
WO2021239666A1 (fr) 2020-05-26 2021-12-02 Diaccurate Procédés thérapeutiques
US20220298236A1 (en) 2021-03-12 2022-09-22 Janssen Biotech, Inc. Safe and Effective Method of Treating Psoriatic Arthritis with Anti-IL23 Specific Antibody
EP4305062A1 (fr) 2021-03-12 2024-01-17 Janssen Biotech, Inc. Méthode de traitement de patients souffrant de polyarthrite psoriasique ayant une réponse inadéquate à une thérapie par tnf avec un anticorps spécifique anti-il23
IL309996A (en) 2021-07-09 2024-03-01 Janssen Biotech Inc Production methods for the production of anti-TNF antibody compositions
AU2022306144A1 (en) 2021-07-09 2024-02-22 Janssen Biotech, Inc. Manufacturing methods for producing anti-tnf antibody compositions
CN117916260A (zh) 2021-07-09 2024-04-19 詹森生物科技公司 用于制备抗il12/il23抗体组合物的制造方法
KR20240099352A (ko) 2021-10-29 2024-06-28 얀센 바이오테크 인코포레이티드 항-il23 특이적 항체로 크론병을 치료하는 방법
AU2022388887A1 (en) 2021-11-15 2024-07-04 Janssen Biotech, Inc. Methods of treating crohn's disease with anti-il23 specific antibody
US20230159633A1 (en) 2021-11-23 2023-05-25 Janssen Biotech, Inc. Method of Treating Ulcerative Colitis with Anti-IL23 Specific Antibody
WO2023118508A1 (fr) 2021-12-23 2023-06-29 Bavarian Nordic A/S Virus mva recombinants pour administration intrapéritonéale pour le traitement du cancer
WO2023187707A1 (fr) 2022-03-30 2023-10-05 Janssen Biotech, Inc. Méthode de traitement du psoriasis léger à modéré avec un anticorps spécifique d'il-23
WO2023223265A1 (fr) 2022-05-18 2023-11-23 Janssen Biotech, Inc. Méthode d'évaluation et de traitement de l'arthrite psoriasique avec un anticorps il23
WO2024110898A1 (fr) 2022-11-22 2024-05-30 Janssen Biotech, Inc. Méthode de traitement de la rectocolite hémorragique avec un anticorps spécifique anti-il23

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US6955900B1 (en) * 1993-02-02 2005-10-18 The Scripps Research Institute Methods for producing polypeptide binding sites, monoclonal antibodies and compositions thereof
WO1994018219A1 (fr) * 1993-02-02 1994-08-18 The Scripps Research Institute Procedes de production de banques d'anticorps utilisant des chaines legeres d'immunoglobulines universelles ou aleatoires

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US6096551A (en) 2000-08-01
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ES2196077T3 (es) 2003-12-16
ATE236992T1 (de) 2003-04-15
DK0779933T3 (da) 2003-07-28
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US5667988A (en) 1997-09-16

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