CA1199292A - Debranching enzyme product, preparation and use thereof - Google Patents
Debranching enzyme product, preparation and use thereofInfo
- Publication number
- CA1199292A CA1199292A CA000401249A CA401249A CA1199292A CA 1199292 A CA1199292 A CA 1199292A CA 000401249 A CA000401249 A CA 000401249A CA 401249 A CA401249 A CA 401249A CA 1199292 A CA1199292 A CA 1199292A
- Authority
- CA
- Canada
- Prior art keywords
- debranching enzyme
- ncib
- enzyme
- strain
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 106
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 106
- 238000002360 preparation method Methods 0.000 title claims description 8
- 229920002472 Starch Polymers 0.000 claims abstract description 29
- 235000019698 starch Nutrition 0.000 claims abstract description 29
- 239000008107 starch Substances 0.000 claims abstract description 28
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims abstract description 26
- 102100022624 Glucoamylase Human genes 0.000 claims abstract description 23
- 241001134780 Bacillus acidopullulyticus Species 0.000 claims abstract description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 37
- 238000000034 method Methods 0.000 claims description 33
- 239000008121 dextrose Substances 0.000 claims description 28
- 230000000694 effects Effects 0.000 claims description 27
- 108090000637 alpha-Amylases Proteins 0.000 claims description 23
- 239000000047 product Substances 0.000 claims description 21
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 14
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 12
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 108010019077 beta-Amylase Proteins 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 235000020357 syrup Nutrition 0.000 claims description 10
- 239000006188 syrup Substances 0.000 claims description 10
- 235000008504 concentrate Nutrition 0.000 claims description 9
- 239000012141 concentrate Substances 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 239000008351 acetate buffer Substances 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
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- 238000004519 manufacturing process Methods 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 239000000413 hydrolysate Substances 0.000 claims description 3
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- 238000011084 recovery Methods 0.000 claims description 2
- 235000000346 sugar Nutrition 0.000 claims description 2
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- 229940088598 enzyme Drugs 0.000 description 85
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 33
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 239000004373 Pullulan Substances 0.000 description 8
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- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 3
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- 239000007640 basal medium Substances 0.000 description 3
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- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 2
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- 235000019764 Soybean Meal Nutrition 0.000 description 2
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- 229940024171 alpha-amylase Drugs 0.000 description 2
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
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- 108010053835 Catalase Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
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- 235000010469 Glycine max Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- DKNPRRRKHAEUMW-UHFFFAOYSA-N Iodine aqueous Chemical compound [K+].I[I-]I DKNPRRRKHAEUMW-UHFFFAOYSA-N 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 241000588915 Klebsiella aerogenes Species 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
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- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000282344 Mellivora capensis Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
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- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
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- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
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- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2457—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01041—Pullulanase (3.2.1.41)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Detergent Compositions (AREA)
- Jellies, Jams, And Syrups (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25595281A | 1981-04-20 | 1981-04-20 | |
| US255,952 | 1981-04-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1199292A true CA1199292A (en) | 1986-01-14 |
Family
ID=22970517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000401249A Expired CA1199292A (en) | 1981-04-20 | 1982-04-19 | Debranching enzyme product, preparation and use thereof |
Country Status (18)
| Country | Link |
|---|---|
| EP (1) | EP0063909B2 (OSRAM) |
| JP (2) | JPS57174089A (OSRAM) |
| KR (1) | KR890002413B1 (OSRAM) |
| AT (1) | ATE28216T1 (OSRAM) |
| AU (1) | AU548122B2 (OSRAM) |
| BE (1) | BE892904A (OSRAM) |
| CA (1) | CA1199292A (OSRAM) |
| CH (1) | CH659825A5 (OSRAM) |
| DE (1) | DE3276707D1 (OSRAM) |
| DK (1) | DK153569C (OSRAM) |
| ES (2) | ES511504A0 (OSRAM) |
| FI (1) | FI79345C (OSRAM) |
| FR (1) | FR2504148A1 (OSRAM) |
| GB (1) | GB2097405B (OSRAM) |
| IE (1) | IE53031B1 (OSRAM) |
| IN (1) | IN155660B (OSRAM) |
| IT (1) | IT1190780B (OSRAM) |
| MX (1) | MX174149B (OSRAM) |
Families Citing this family (79)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58183092A (ja) * | 1982-04-19 | 1983-10-26 | ノボ・インダストリ・アクテイ−ゼルスカブ | 枝切り酵素 |
| CA1211314A (en) * | 1983-05-20 | 1986-09-16 | William F. Line | Production of dextrose and maltose syrups using an enzyme derived from rice |
| IE58094B1 (en) * | 1983-09-11 | 1993-06-30 | Gist Brocades Nv | Novel enzyme product and its use in the saccharification of starch |
| JPS60186283A (ja) * | 1984-03-07 | 1985-09-21 | Agency Of Ind Science & Technol | 微生物による新規な耐熱性プルラナ−ゼａの製造法 |
| US4673633A (en) * | 1984-06-28 | 1987-06-16 | Cooperbiomedical, Inc. | Method of determining cystic fibrosis ciliostatic factor |
| US4628028A (en) * | 1985-05-23 | 1986-12-09 | Cpc International Inc. | Novel thermostable pullulanase enzyme and method for its production |
| GB8609288D0 (en) * | 1986-04-16 | 1986-05-21 | Ici Plc | Debranching enzyme |
| US5234823A (en) * | 1986-06-30 | 1993-08-10 | Novo Industri A/S | Chimeric enzymes |
| US5055403A (en) * | 1989-06-26 | 1991-10-08 | Enzyme Bio-Systems, Ltd. | Thermoduric and aciduric pullulanase enzyme and method for its production |
| EP0405283A3 (en) * | 1989-06-26 | 1991-03-06 | Enzyme Bio-Systems Ltd. | Novel thermoduric and aciduric pullulanase enzyme and method for its production |
| DK47291D0 (da) * | 1991-03-15 | 1991-03-15 | Novo Nordisk As | Pullulanase |
| JPH05236959A (ja) * | 1992-02-28 | 1993-09-17 | Yoshiyuki Takasaki | プルラナーゼ、その製造法及び該酵素を用いる澱粉の 糖化法 |
| FR2688800B1 (fr) * | 1992-03-19 | 1995-06-16 | Roquette Freres | Procede de preparation de polysaccharides peu digestibles, eventuellement hydrogenes. |
| BE1006483A3 (fr) * | 1992-12-28 | 1994-09-13 | Solvay | Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase, utilisations et compositions comprenant celle-ci. |
| EP0605040B1 (fr) * | 1992-12-28 | 1999-08-11 | Genencor International, Inc. | Pullulanase, microorganismes la produisant, procédés de préparation de cette pullulanase et utilisations de celle-ci |
| ES2250969T3 (es) | 1994-03-29 | 2006-04-16 | Novozymes A/S | Amilasa alcalina de bacilo. |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| EP0904360B1 (en) | 1996-04-30 | 2013-07-31 | Novozymes A/S | alpha-AMYLASE MUTANTS |
| EP2206768B1 (en) | 1997-10-13 | 2015-04-01 | Novozymes A/S | Alpha-amylase mutants |
| WO1999023211A1 (en) | 1997-10-30 | 1999-05-14 | Novo Nordisk A/S | α-AMYLASE MUTANTS |
| AU757935B2 (en) | 1998-02-27 | 2003-03-13 | Novozymes A/S | Maltogenic alpha-amylase variants |
| WO2000060059A2 (en) | 1999-03-30 | 2000-10-12 | NovozymesA/S | Alpha-amylase variants |
| CN100523181C (zh) | 1999-03-31 | 2009-08-05 | 诺维信公司 | 具有碱性α-淀粉酶活性的多肽及其编码核酸 |
| EP2011864B1 (en) | 1999-03-31 | 2014-12-31 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
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| US20040091994A1 (en) | 2000-10-13 | 2004-05-13 | Carsten Andersen | Alpha-amylase variant with altered properties |
| AU2002254876A1 (en) | 2001-05-15 | 2002-11-25 | Novozymes A/S | Alpha-amylase variant with altered properties |
| AU2004252572B2 (en) | 2003-06-25 | 2011-09-08 | Novozymes A/S | Polypeptides having alpha-amylase activity and polypeptides encoding same |
| BRPI0512776A (pt) | 2004-07-05 | 2008-04-08 | Novozymes As | variante de uma alfa-amilase tipo termamil originária, construto de dna, vetor de expressão recombinante, célula, composição, aditivo de detergente, composição detergente, composição de lavagem de roupa manual ou automática, uso de uma variante de alfa-amilase ou composição, e, método de produzir uma variante |
| US20080032024A1 (en) | 2004-08-02 | 2008-02-07 | Lars Beier | Maltogenic Alpha-Amylase Variants |
| US20080280328A1 (en) | 2005-11-18 | 2008-11-13 | Novozymes A/S | Glucoamylase Variants |
| JP5520828B2 (ja) | 2007-11-05 | 2014-06-11 | ダニスコ・ユーエス・インク | 改変される特徴を有するバシルス種（Ｂａｃｉｌｌｕｓｓｐ．）ＴＳ‐２３アルファ‐アミラーゼ変異体 |
| AR069168A1 (es) | 2007-11-05 | 2010-01-06 | Danisco Us Inc Genencor Div | Variantes de alfa -amilasas con propiedades alteradas |
| JP2011510681A (ja) | 2008-02-04 | 2011-04-07 | ダニスコ・ユーエス・インク | 改変された特性をもつｔｓ２３アルファ‐アミラーゼ変異体 |
| JP5492879B2 (ja) | 2008-06-06 | 2014-05-14 | ダニスコ・ユーエス・インク | 改善された性質を有するゲオバチルスステアロセレモフィリス（Ｇｅｏｂａｃｉｌｌｕｓｓｔｅａｒｏｔｈｅｒｍｏｐｈｉｌｕｓ）アルファアミラーゼ（ＡｍｙＳ）変異体 |
| EP2337837B2 (en) | 2008-09-25 | 2016-10-26 | Danisco US Inc. | Alpha-amylase blends and methods for using said blends |
| EP2414515A2 (en) | 2009-04-01 | 2012-02-08 | Danisco US Inc. | Cleaning system comprising an alpha-amylase and a protease |
| US8545907B2 (en) | 2009-08-07 | 2013-10-01 | Danisco Us Inc. | Alpha-amylase blend for starch processing and method of use thereof |
| CN102933227A (zh) | 2009-12-22 | 2013-02-13 | 诺维信公司 | 包含增强性多肽和淀粉降解酶的组合物及其用途 |
| EP2521773A1 (en) | 2010-01-04 | 2012-11-14 | Novozymes A/S | Alpha-amylases |
| EP2534236B1 (en) | 2010-02-10 | 2018-05-30 | Novozymes A/S | Variants and compositions comprising variants with high stability in presence of a chelating agent |
| EP2357220A1 (en) | 2010-02-10 | 2011-08-17 | The Procter & Gamble Company | Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent |
| KR20140056237A (ko) | 2011-06-30 | 2014-05-09 | 노보자임스 에이/에스 | 알파-아밀라제 변이체 |
| JP2014528241A (ja) | 2011-09-29 | 2014-10-27 | ダニスコ・ユーエス・インク | 高濃度デンプン粒の液化及び糖化 |
| CA3253390A1 (en) | 2011-10-17 | 2025-06-17 | Novozymes A/S | Variants of alpha-amylase and the polynucleotides encoding them |
| KR102046075B1 (ko) | 2011-10-17 | 2019-11-18 | 노보자임스 에이/에스 | 알파-아밀라아제 변이체 및 이를 암호화하는 폴리뉴클레오티드 |
| WO2013148152A1 (en) | 2012-03-28 | 2013-10-03 | Danisco Us Inc. | Method for making high maltose syrup |
| CA2878988A1 (en) | 2012-08-16 | 2014-02-20 | Danisco Us Inc. | Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification |
| CA2893270C (en) | 2012-12-11 | 2024-01-02 | Danisco Us Inc. | Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof |
| CN104903461A (zh) | 2012-12-20 | 2015-09-09 | 丹尼斯科美国公司 | 将支链淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法 |
| EP3022300B1 (en) | 2013-07-17 | 2018-07-11 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
| DK3097192T3 (en) | 2014-01-22 | 2018-11-19 | Novozymes As | PULLULANASE VARIATIONS AND POLYNUCLEOTIDES CODING THEM |
| WO2015143144A1 (en) | 2014-03-19 | 2015-09-24 | Novozymes A/S | Method for enhancing activity of an x143 polypeptide |
| WO2015157656A1 (en) | 2014-04-10 | 2015-10-15 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| WO2016087327A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains |
| CN108368528A (zh) | 2015-10-14 | 2018-08-03 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| MX2018007485A (es) | 2015-12-30 | 2018-08-01 | Novozymes As | Variantes de enzimas y polinucleotidos que las codifican. |
| WO2017191160A1 (en) | 2016-05-03 | 2017-11-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding the same |
| US11186833B2 (en) | 2016-05-09 | 2021-11-30 | Novozymes A/S | Variant polypeptides with improved performance and use of the same |
| DK3478831T3 (da) | 2016-06-30 | 2021-04-19 | Danisco Us Inc | Asparaginproteaser |
| EP3272767B1 (en) | 2016-07-21 | 2020-11-25 | Fornia BioSolutions, Inc. | G24 glucoamylase compositions and methods |
| DK3487996T5 (da) | 2016-07-21 | 2024-09-02 | Novozymes As | Serinproteasevarianter og polynukleotider, der koder for samme |
| WO2018017105A1 (en) | 2016-07-21 | 2018-01-25 | Fornia Biosolutions, Inc. | G24 glucoamylase compositions and methods |
| US11891645B2 (en) | 2016-07-21 | 2024-02-06 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
| US9598680B1 (en) | 2016-08-05 | 2017-03-21 | Fornia Biosolutions, Inc. | G16 glucoamylase compositions and methods |
| EP3548615A1 (en) | 2016-11-29 | 2019-10-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| US20190330577A1 (en) | 2016-12-21 | 2019-10-31 | Dupont Nutrition Biosciences Aps | Methods of using thermostable serine proteases |
| US20200015499A1 (en) | 2017-03-15 | 2020-01-16 | Dupont Nutrition Biosciences Aps | Trypsin-like serine proteases and uses thereof |
| RU2019132448A (ru) | 2017-03-15 | 2021-04-15 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения сериновой протеазы архей |
| US11279921B2 (en) | 2017-04-11 | 2022-03-22 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| US20200181663A1 (en) | 2017-08-30 | 2020-06-11 | Novozymes A/S | Combined use of at least one endoprotease and at least one exo-protease in an ssf process for improving ethanol yield |
| ES2955269T3 (es) | 2017-12-08 | 2023-11-29 | Novozymes As | Variantes de alfa-amilasa y polinucleótidos que codifican las mismas |
| BR112020011173A2 (pt) | 2017-12-08 | 2020-11-17 | Novozymes A/S | variante de alfa-amilase, polinucleotídeo, composição, construto de ácido nucleico, vetor de expressão, célula hospedeira, método de produção de uma variante de alfa-amilase, uso da variante, e, processo para produção de um xarope a partir de material contendo amido. |
| US11377648B2 (en) | 2018-04-09 | 2022-07-05 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
| US11535836B2 (en) | 2018-12-21 | 2022-12-27 | Fornia Biosolutions, Inc. | Variant G6P G7P glucoamylase compositions and methods |
| WO2020188095A1 (en) | 2019-03-21 | 2020-09-24 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| US20230142991A1 (en) | 2020-02-10 | 2023-05-11 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3565765A (en) * | 1966-12-27 | 1971-02-23 | Cpc International Inc | Preparation of high maltose conversion products |
| US3827940A (en) * | 1968-04-01 | 1974-08-06 | Hayashibara Co | Preparation of alpha-1,6-glucosidase |
| JPS543937B1 (OSRAM) * | 1968-11-18 | 1979-02-28 | ||
| JPS543938B1 (OSRAM) * | 1968-11-26 | 1979-02-28 | ||
| JPS529739B1 (OSRAM) * | 1969-04-09 | 1977-03-18 | ||
| JPS536232B2 (OSRAM) * | 1974-01-11 | 1978-03-06 | ||
| US4011139A (en) * | 1974-11-26 | 1977-03-08 | Standard Brands Incorporated | Process for producing α-1,6 glucosidases using thermophilic microorganisms |
-
1981
- 1981-06-10 JP JP56088319A patent/JPS57174089A/ja active Granted
-
1982
- 1982-04-19 BE BE6/47645A patent/BE892904A/fr not_active IP Right Cessation
- 1982-04-19 DK DK172882A patent/DK153569C/da not_active IP Right Cessation
- 1982-04-19 ES ES511504A patent/ES511504A0/es active Granted
- 1982-04-19 AT AT82302001T patent/ATE28216T1/de not_active IP Right Cessation
- 1982-04-19 GB GB8211280A patent/GB2097405B/en not_active Expired
- 1982-04-19 CA CA000401249A patent/CA1199292A/en not_active Expired
- 1982-04-19 IT IT20806/82A patent/IT1190780B/it active
- 1982-04-19 FI FI821355A patent/FI79345C/fi not_active IP Right Cessation
- 1982-04-19 EP EP82302001A patent/EP0063909B2/en not_active Expired - Lifetime
- 1982-04-19 DE DE8282302001T patent/DE3276707D1/de not_active Expired
- 1982-04-19 KR KR8201726A patent/KR890002413B1/ko not_active Expired
- 1982-04-19 IE IE907/82A patent/IE53031B1/en not_active IP Right Cessation
- 1982-04-19 AU AU82808/82A patent/AU548122B2/en not_active Ceased
- 1982-04-20 IN IN434/CAL/82A patent/IN155660B/en unknown
- 1982-04-20 CH CH2393/82A patent/CH659825A5/de not_active IP Right Cessation
- 1982-04-20 MX MX026679A patent/MX174149B/es unknown
- 1982-04-20 FR FR8206786A patent/FR2504148A1/fr active Granted
-
1983
- 1983-03-14 ES ES520561A patent/ES8500994A1/es not_active Expired
-
1985
- 1985-08-09 JP JP60174391A patent/JPS6143994A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IE820907L (en) | 1982-10-20 |
| ES520561A0 (es) | 1984-11-01 |
| JPS6143994A (ja) | 1986-03-03 |
| FR2504148A1 (fr) | 1982-10-22 |
| AU548122B2 (en) | 1985-11-21 |
| FI821355L (fi) | 1982-10-21 |
| ES8307291A1 (es) | 1983-06-16 |
| DK153569C (da) | 1988-12-05 |
| KR830010192A (ko) | 1983-12-26 |
| DK153569B (da) | 1988-07-25 |
| IN155660B (OSRAM) | 1985-02-16 |
| DK172882A (da) | 1982-12-29 |
| IT8220806A0 (it) | 1982-04-19 |
| FI821355A0 (fi) | 1982-04-19 |
| FI79345C (fi) | 1989-12-11 |
| ATE28216T1 (de) | 1987-07-15 |
| FI79345B (fi) | 1989-08-31 |
| AU8280882A (en) | 1982-10-28 |
| DE3276707D1 (en) | 1987-08-13 |
| ES8500994A1 (es) | 1984-11-01 |
| MX174149B (es) | 1994-04-25 |
| FR2504148B1 (OSRAM) | 1984-05-18 |
| JPS57174089A (en) | 1982-10-26 |
| EP0063909B1 (en) | 1987-07-08 |
| EP0063909A1 (en) | 1982-11-03 |
| IE53031B1 (en) | 1988-05-11 |
| EP0063909B2 (en) | 1990-09-12 |
| IT1190780B (it) | 1988-02-24 |
| CH659825A5 (de) | 1987-02-27 |
| GB2097405B (en) | 1984-08-15 |
| GB2097405A (en) | 1982-11-03 |
| BE892904A (fr) | 1982-10-19 |
| JPS6225036B2 (OSRAM) | 1987-06-01 |
| ES511504A0 (es) | 1983-06-16 |
| KR890002413B1 (ko) | 1989-07-03 |
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