BRPI1015952B8 - Método de introdução de uma nuclease sequência-específica (ssn) em uma célula vegetal - Google Patents

Método de introdução de uma nuclease sequência-específica (ssn) em uma célula vegetal

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Publication number
BRPI1015952B8
BRPI1015952B8 BRPI1015952A BRPI1015952A BRPI1015952B8 BR PI1015952 B8 BRPI1015952 B8 BR PI1015952B8 BR PI1015952 A BRPI1015952 A BR PI1015952A BR PI1015952 A BRPI1015952 A BR PI1015952A BR PI1015952 B8 BRPI1015952 B8 BR PI1015952B8
Authority
BR
Brazil
Prior art keywords
sequence
specific nuclease
introduction
plant cell
ssn
Prior art date
Application number
BRPI1015952A
Other languages
English (en)
Inventor
Samuel Jayakumar
Petolino Joseph
Samboju Narasimha
Webb Steven
Yau Kerrm
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BRPI1015952A2 publication Critical patent/BRPI1015952A2/pt
Publication of BRPI1015952B1 publication Critical patent/BRPI1015952B1/pt
Publication of BRPI1015952B8 publication Critical patent/BRPI1015952B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)

Abstract

MÉTODOS DE INTRODUÇÃO DE UMA NUCLEASE ESPECÍFICA À SEQUÊNCIA (NES) EM UMA CÉLULA VEGETAL. A presente invenção refere-se a métodos para introdução de uma nuclease específica á sequência em uma célula vegetal que compreende uma parede celular. São proporcionados métodos para modificar geneticamente ou modificar de outro modo plantas e para tratar ou prevenir doença em células vegetais que compreendem uma parede celular.
BRPI1015952A 2009-04-07 2010-04-07 Método de introdução de uma nuclease sequência-específica (ssn) em uma célula vegetal BRPI1015952B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16738909P 2009-04-07 2009-04-07
US61/167,389 2009-04-07
US2010118077 2010-10-14

Publications (3)

Publication Number Publication Date
BRPI1015952A2 BRPI1015952A2 (pt) 2015-09-01
BRPI1015952B1 BRPI1015952B1 (pt) 2018-12-04
BRPI1015952B8 true BRPI1015952B8 (pt) 2022-06-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1015952A BRPI1015952B8 (pt) 2009-04-07 2010-04-07 Método de introdução de uma nuclease sequência-específica (ssn) em uma célula vegetal

Country Status (20)

Country Link
US (2) US9187755B2 (pt)
EP (1) EP2417262B1 (pt)
JP (3) JP5755636B2 (pt)
KR (1) KR101701534B1 (pt)
CN (1) CN102369287B (pt)
AR (1) AR076225A1 (pt)
AU (1) AU2010234539B2 (pt)
BR (1) BRPI1015952B8 (pt)
CA (1) CA2757831C (pt)
DK (1) DK2417262T3 (pt)
ES (1) ES2539327T3 (pt)
HR (1) HRP20150564T1 (pt)
HU (1) HUE026053T2 (pt)
IL (1) IL214880A (pt)
PT (1) PT2417262E (pt)
RU (3) RU2556376C2 (pt)
SI (1) SI2417262T1 (pt)
TW (1) TWI576432B (pt)
WO (1) WO2010118077A1 (pt)
ZA (1) ZA201106262B (pt)

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Publication number Publication date
ES2539327T3 (es) 2015-06-29
BRPI1015952A2 (pt) 2015-09-01
JP6275668B2 (ja) 2018-02-07
JP2017200479A (ja) 2017-11-09
SI2417262T1 (sl) 2015-08-31
BRPI1015952B1 (pt) 2018-12-04
HRP20150564T1 (hr) 2015-08-28
EP2417262A1 (en) 2012-02-15
CA2757831A1 (en) 2010-10-14
RU2664865C2 (ru) 2018-08-23
RU2556376C2 (ru) 2015-07-10
PT2417262E (pt) 2015-07-23
HUE026053T2 (en) 2016-05-30
EP2417262B1 (en) 2015-05-20
RU2017104660A (ru) 2018-08-15
AR076225A1 (es) 2011-05-26
AU2010234539A1 (en) 2011-09-15
AU2010234539B2 (en) 2015-04-09
JP2015171370A (ja) 2015-10-01
IL214880A0 (en) 2011-11-30
CA2757831C (en) 2017-09-26
RU2612156C2 (ru) 2017-03-02
US9719108B2 (en) 2017-08-01
US20100311168A1 (en) 2010-12-09
KR101701534B1 (ko) 2017-02-01
WO2010118077A1 (en) 2010-10-14
US9187755B2 (en) 2015-11-17
RU2015120061A (ru) 2015-10-20
CN102369287B (zh) 2014-09-17
KR20120003933A (ko) 2012-01-11
RU2011144872A (ru) 2013-05-20
RU2017104660A3 (pt) 2018-08-15
JP2012523234A (ja) 2012-10-04
JP5755636B2 (ja) 2015-07-29
TWI576432B (zh) 2017-04-01
DK2417262T3 (da) 2015-06-22
ZA201106262B (en) 2012-10-31
CN102369287A (zh) 2012-03-07
JP6513127B2 (ja) 2019-05-15
US20170022521A1 (en) 2017-01-26
IL214880A (en) 2016-04-21
TW201040277A (en) 2010-11-16

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