HRP20150564T1 - Nanoäśesticama posredovana sekvenca specifiäśnih nukleaza - Google Patents
Nanoäśesticama posredovana sekvenca specifiäśnih nukleaza Download PDFInfo
- Publication number
- HRP20150564T1 HRP20150564T1 HRP20150564TT HRP20150564T HRP20150564T1 HR P20150564 T1 HRP20150564 T1 HR P20150564T1 HR P20150564T T HRP20150564T T HR P20150564TT HR P20150564 T HRP20150564 T HR P20150564T HR P20150564 T1 HRP20150564 T1 HR P20150564T1
- Authority
- HR
- Croatia
- Prior art keywords
- nanoparticle
- nanoparticles
- ssn
- cell
- dendrimer
- Prior art date
Links
- 239000002105 nanoparticle Substances 0.000 title claims 35
- 101710163270 Nuclease Proteins 0.000 title claims 3
- 230000001404 mediated effect Effects 0.000 title 1
- 238000000034 method Methods 0.000 claims 18
- 210000004027 cell Anatomy 0.000 claims 14
- 241000196324 Embryophyta Species 0.000 claims 10
- 239000000412 dendrimer Substances 0.000 claims 8
- 229920000736 dendritic polymer Polymers 0.000 claims 8
- 210000002421 cell wall Anatomy 0.000 claims 7
- 229920000962 poly(amidoamine) Polymers 0.000 claims 6
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 claims 4
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 claims 4
- 210000002706 plastid Anatomy 0.000 claims 4
- 239000011248 coating agent Substances 0.000 claims 3
- 238000000576 coating method Methods 0.000 claims 3
- 238000010521 absorption reaction Methods 0.000 claims 2
- 210000003763 chloroplast Anatomy 0.000 claims 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 2
- 239000010931 gold Substances 0.000 claims 2
- 229910052737 gold Inorganic materials 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 230000001172 regenerating effect Effects 0.000 claims 2
- 241001149092 Arabidopsis sp. Species 0.000 claims 1
- 235000017060 Arachis glabrata Nutrition 0.000 claims 1
- 244000105624 Arachis hypogaea Species 0.000 claims 1
- 235000010777 Arachis hypogaea Nutrition 0.000 claims 1
- 235000018262 Arachis monticola Nutrition 0.000 claims 1
- 240000002791 Brassica napus Species 0.000 claims 1
- 235000006008 Brassica napus var napus Nutrition 0.000 claims 1
- 240000000385 Brassica napus var. napus Species 0.000 claims 1
- 229920000742 Cotton Polymers 0.000 claims 1
- 244000000626 Daucus carota Species 0.000 claims 1
- 235000002767 Daucus carota Nutrition 0.000 claims 1
- 108010010803 Gelatin Proteins 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 241000219146 Gossypium Species 0.000 claims 1
- 206010020649 Hyperkeratosis Diseases 0.000 claims 1
- 244000061176 Nicotiana tabacum Species 0.000 claims 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims 1
- 241000511986 Oryza sp. Species 0.000 claims 1
- 235000003846 Ricinus Nutrition 0.000 claims 1
- 241000322381 Ricinus <louse> Species 0.000 claims 1
- 240000000111 Saccharum officinarum Species 0.000 claims 1
- 235000007201 Saccharum officinarum Nutrition 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 1
- 101710185494 Zinc finger protein Proteins 0.000 claims 1
- 102100023597 Zinc finger protein 816 Human genes 0.000 claims 1
- 239000011162 core material Substances 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 210000000172 cytosol Anatomy 0.000 claims 1
- 238000001212 derivatisation Methods 0.000 claims 1
- 239000008273 gelatin Substances 0.000 claims 1
- 229920000159 gelatin Polymers 0.000 claims 1
- 235000019322 gelatine Nutrition 0.000 claims 1
- 235000011852 gelatine desserts Nutrition 0.000 claims 1
- 239000002122 magnetic nanoparticle Substances 0.000 claims 1
- 210000001161 mammalian embryo Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 230000000442 meristematic effect Effects 0.000 claims 1
- 239000005543 nano-size silicon particle Substances 0.000 claims 1
- -1 nanomatrices Substances 0.000 claims 1
- 239000002073 nanorod Substances 0.000 claims 1
- 239000002078 nanoshell Substances 0.000 claims 1
- 239000002077 nanosphere Substances 0.000 claims 1
- 235000020232 peanut Nutrition 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 239000002096 quantum dot Substances 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 239000004065 semiconductor Substances 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 claims 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims 1
- 239000010937 tungsten Substances 0.000 claims 1
- 229910052721 tungsten Inorganic materials 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
- C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (22)
1. Postupak uvođenja sekvenca specifične nukleaze (SSN) u stanicu biljke, gdje postupak obuhvaća:
oblaganje nanočestice sa SSN, naznačeno time da je nanočestica dendrimerna nanomatrica ili nanočestica koja je obložena sa u stanicu penetrirajućim peptidom;
stavljanje stanice koja ima stanični zid i obložene nanočestice u međusobni kontakt; i
dopuštanje unosa nanočestice i SSN u stanicu biljke koja sadrži stanični zid.
2. Postupak prema zahtjevu 1, koji nadalje obuhvaća prvo pribavljanje biljne stanice koja ima stanični zid.
3. Postupak prema zahtjevu 2, naznačen time da oblaganje nanočestice sa SSN obuhvaća imobilizaciju SSN preko ne-kovalentne apsorpcije na površini nanočestice.
4. Postupak prema zahtjevu 2, koji nadalje obuhvaća apsorpciju SSN u nanočesticu.
5. Postupak prema zahtjevu 2, koji nadalje obuhvaća dopuštanje unosa nanočestice u odjeljak biljne stanice koji sadrži stanični zid.
6. Postupak prema bilo kojem od zahtjeva 1 do 5, koji nadalje obuhvaća oblaganje nanočestice sa sub-staničnim na odjeljak ciljanim proteinom.
7. Postupak prema zahtjevu 5, naznačen time da je odjeljak odabran iz grupe koja se sastoji od citosola, jezgre, tonoplasta, plastida, etioplasta, kromoplast leukoplasta, elaioplasta, proteinoplasta, amiloplasta, kloroplasa i lumena dvostruke membrane.
8. Postupak prema bilo kojem od predhodnih zahtjeva, naznačen time da je biljna stanica koja sadrži stanični zid odabrana između stanica biljaka izabranih iz grupe koja se sastoji od duhana, mrkve, kukuruza, canola uljane repice, uljane repice, pamuka, kikirikija, soje, Oryza sp., Arabidopsis sp., Ricinus sp., i šećerne trske.
9. Postupak prema bilo kojem od predhodnih zahtjeva, naznačen time da je biljna stanica iz tkiva izabranih iz grupe koja se sastoji od embrija, meristematic, kalusa, peluda, lišća, prašnika, korijena, vršaka korijena, cvijeta, sjemenja, mahuna i stabljika.
10. Postupak prema bilo kojem od predhodnih zahtjeva, naznačen time da su nanočestice izabrane iz grupe koja se sastoji od zlatnih nanočestice, zlatom obloženih nanočestica, poroznih nanočestica, mezoporoznih nanočestica, silikonskih nanočestica, polimernih nanočestica, volframovih nanočestica, želatin nanočestica, nano školjki, nano jezgri, nano sfera, nano šipki, magnetskih nanočestica, poluvodičkih nanočestica, kvantnih točkica, nanomatrica, dendrimernih nanomatrica i njihovih kombinacija.
11. Postupak prema zahtjevu 2, koji nadalje obuhvaća derivatizaciju površine nanočestice.
12. Postupak prema zahtjevu 2, naznačen time da je SSN cink finger nukleaza koja se sastoji od cink finger proteina sa sekvenca-neovisnom nukleaznom domenom poželjno izvedenom iz restrikcijske endonukleaze Fokl tipa IIS.
13. Postupak prema zahtjevu 12, koji nadalje obuhvaća odabir stanica koje su stabilno integrirale ZFN.
14. Postupak prema zahtjevu 13, naznačen time da su odabrane stanice regenerabilne stanice.
15. Postupak prema zahtjevu 14, koji nadalje obuhvaća regeneriranje biljke iz regenerabilnih stanica.
16. Postupak prema bilo kojem od predhodnih zahtjeva, naznačen time da su nanočestice multifunkcionalizirane nanočestice.
17. Upotreba nanočestice obložene sa SSN u proizvodnji transgenične biljne stanice koja ima stanični zid, naznačeno time da je nanočestica dendrimerna nanomatrica ili nanočestica koja je obložena sa u stanicu penetrirajućim peptidom.
18. Kompozicija koja obuhvaća nanočesticu obloženu sa SSN, naznačena time da je nanočestica dendrimerna nanomatrica ili nanočestica koja je obložena sa u stanicu penetrirajućim peptidom.
19. Bilja stanica koja ima stanični zid a tranfektirana je nanočesticom obloženom sa SSN, naznačeno time da je nanočestica dendrimerna nanomatrica ili nanočestica koja je obložena sa u stanicu penetrirajućim peptidom.
20. Postupak prema bilo kojem od zahtjeva 1-16, naznačeno time da je nanočestica poliamidoamin (PAMAM) dendrimerna nanomatrica.
21. Upotreba prema zahtjevu 17, naznačeno time da je nanočestica poliamidoamin (PAMAM) dendrimerna nanomatrica.
22. Kompozicija prema zahtjevu 18, naznačena time da je nanočestica poliamidoamin (PAMAM) dendrimerna nanomatrica.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16738909P | 2009-04-07 | 2009-04-07 | |
| EP10714403.2A EP2417262B1 (en) | 2009-04-07 | 2010-04-07 | Nanoparticle mediated delivery of sequence specific nucleases |
| PCT/US2010/030155 WO2010118077A1 (en) | 2009-04-07 | 2010-04-07 | Nanoparticle mediated delivery of sequence specific nucleases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20150564T1 true HRP20150564T1 (hr) | 2015-08-28 |
Family
ID=42173819
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20150564TT HRP20150564T1 (hr) | 2009-04-07 | 2015-05-27 | Nanoäśesticama posredovana sekvenca specifiäśnih nukleaza |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US9187755B2 (hr) |
| EP (1) | EP2417262B1 (hr) |
| JP (3) | JP5755636B2 (hr) |
| KR (1) | KR101701534B1 (hr) |
| CN (1) | CN102369287B (hr) |
| AR (1) | AR076225A1 (hr) |
| AU (1) | AU2010234539B2 (hr) |
| BR (1) | BRPI1015952B8 (hr) |
| CA (1) | CA2757831C (hr) |
| DK (1) | DK2417262T3 (hr) |
| ES (1) | ES2539327T3 (hr) |
| HR (1) | HRP20150564T1 (hr) |
| HU (1) | HUE026053T2 (hr) |
| IL (1) | IL214880A (hr) |
| PT (1) | PT2417262E (hr) |
| RU (3) | RU2612156C2 (hr) |
| SI (1) | SI2417262T1 (hr) |
| TW (1) | TWI576432B (hr) |
| WO (1) | WO2010118077A1 (hr) |
| ZA (1) | ZA201106262B (hr) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9339539B2 (en) * | 2008-11-07 | 2016-05-17 | Hidaca Limited | Transfection with magnetic nanoparticles |
| WO2012161764A1 (en) * | 2011-02-16 | 2012-11-29 | Fox Daniel N | Magnetic sorting of mammalian sperm having damaged membranes |
| WO2012129443A2 (en) * | 2011-03-23 | 2012-09-27 | Dow Agrosciences Llc | Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants |
| CN102250944B (zh) * | 2011-06-02 | 2012-10-31 | 南京林业大学 | 一种CdSe/ZnS量子点与植物细胞的共孵育方法 |
| AU2012325723A1 (en) * | 2011-10-21 | 2014-05-15 | Stemgenics, Inc | Functionalized nanoparticles for intracellular delivery of biologically active molecules |
| EP2861737B1 (en) | 2012-06-19 | 2019-04-17 | Regents Of The University Of Minnesota | Gene targeting in plants using dna viruses |
| US20160145631A1 (en) * | 2013-06-14 | 2016-05-26 | Cellectis | Methods for non-transgenic genome editing in plants |
| WO2014204725A1 (en) * | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| CA3109801C (en) | 2013-08-22 | 2024-01-09 | Andrew Cigan | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
| JP6595459B2 (ja) | 2013-09-23 | 2019-10-23 | レンセラール ポリテクニック インスティチュート | 種々の細胞集団におけるナノ粒子媒介遺伝子送達、ゲノム編集およびリガンド標的化修飾 |
| KR20160089527A (ko) | 2013-12-12 | 2016-07-27 | 더 브로드 인스티튜트, 인코퍼레이티드 | 게놈 편집을 위한 ｃｒｉｓｐｒ-ｃａｓ 시스템 및 조성물의 전달, 용도 및 치료적 응용 |
| WO2015089354A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
| WO2015089486A2 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
| JP6455912B2 (ja) * | 2014-03-24 | 2019-01-23 | 国立研究開発法人産業技術総合研究所 | 人工ヌクレアーゼの細胞への導入方法 |
| AU2015288157A1 (en) | 2014-07-11 | 2017-01-19 | E. I. Du Pont De Nemours And Company | Compositions and methods for producing plants resistant to glyphosate herbicide |
| CN108064129A (zh) | 2014-09-12 | 2018-05-22 | 纳幕尔杜邦公司 | 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法 |
| EP3985115A1 (en) | 2014-12-12 | 2022-04-20 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
| US20190024065A1 (en) * | 2015-02-06 | 2019-01-24 | Cellectis | Primary hematopoietic cells genetically engineered by slow release of nucleic acids using nanoparticles |
| WO2016143223A1 (ja) * | 2015-03-09 | 2016-09-15 | 株式会社島津製作所 | 外部環境変化に対する耐性が向上した固定化プロテアーゼ |
| KR102194612B1 (ko) * | 2015-03-16 | 2020-12-23 | 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 | 비-유전성 물질을 이용한 식물 게놈의 부위-특이적인 변형 방법 |
| CN107567499A (zh) | 2015-03-27 | 2018-01-09 | 纳幕尔杜邦公司 | 大豆u6核小rna基因启动子及其在植物小rna基因的组成型表达中的用途 |
| WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US20170000743A1 (en) * | 2015-07-02 | 2017-01-05 | Vindico NanoBio Technology Inc. | Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles |
| US10456452B2 (en) | 2015-07-02 | 2019-10-29 | Poseida Therapeutics, Inc. | Compositions and methods for improved encapsulation of functional proteins in polymeric vesicles |
| US11110168B2 (en) | 2016-03-15 | 2021-09-07 | North Carolina State University | Nanoparticles, controlled-release dosage forms, and methods for delivering an immunotherapeutic agent |
| US11213594B2 (en) | 2016-04-29 | 2022-01-04 | Poseida Therapeutics, Inc. | Poly(histidine)-based micelles for complexation and delivery of proteins and nucleic acids |
| US20190233820A1 (en) * | 2016-10-11 | 2019-08-01 | Stemgenics, Inc. | Nanoparticles functionalized with gene editing tools and related methods |
| EP4256951A3 (en) | 2016-11-04 | 2023-12-06 | Flagship Pioneering Innovations V. Inc. | Novel plant cells, plants, and seeds |
| US10975388B2 (en) | 2016-12-14 | 2021-04-13 | Ligandal, Inc. | Methods and compositions for nucleic acid and protein payload delivery |
| EP3392339A1 (en) | 2017-04-18 | 2018-10-24 | Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen | Improved genome editing in plant cells |
| RU2663347C1 (ru) * | 2017-07-12 | 2018-08-03 | Общество с ограниченной ответственностью "Дока-Генные Технологии" | Способ доставки биологически активных макромолекул в клетки растений |
| AU2019255725A1 (en) * | 2018-04-18 | 2020-12-10 | Ligandal, Inc. | Methods and compositions for genome editing |
| US11518999B1 (en) | 2018-11-09 | 2022-12-06 | Inari Agriculture Technology, Inc. | Plant transformation |
| JP2022514493A (ja) | 2018-12-14 | 2022-02-14 | パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド | ゲノム編集のための新規なｃｒｉｓｐｒ－ｃａｓシステム |
| CN111363013B (zh) * | 2018-12-25 | 2021-11-19 | 深圳先进技术研究院 | 多组分纳米颗粒簇的构建方法 |
| US11692197B2 (en) | 2019-05-06 | 2023-07-04 | Inari Agriculture Technology, Inc. | Delivery of biological molecules to plant cells |
| KR20220052905A (ko) | 2019-06-25 | 2022-04-28 | 이나리 아그리컬쳐 테크놀로지, 인크. | 개선된 상동성 의존적 수선 게놈 편집 |
| AU2022375817A1 (en) | 2021-11-01 | 2024-05-09 | Flagship Pioneering Innovations Vii, Llc | Polynucleotides for modifying organisms |
| WO2023141540A2 (en) | 2022-01-20 | 2023-07-27 | Flagship Pioneering Innovations Vii, Llc | Polynucleotides for modifying organisms |
| EP4299739A1 (en) | 2022-06-30 | 2024-01-03 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| EP4299733A1 (en) | 2022-06-30 | 2024-01-03 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| WO2024005863A1 (en) | 2022-06-30 | 2024-01-04 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
| WO2024005864A1 (en) | 2022-06-30 | 2024-01-04 | Inari Agriculture Technology, Inc. | Compositions, systems, and methods for genome editing |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050059031A1 (en) * | 2000-10-06 | 2005-03-17 | Quantum Dot Corporation | Method for enhancing transport of semiconductor nanocrystals across biological membranes |
| DE10254166A1 (de) * | 2002-11-20 | 2004-06-03 | Icon Genetics Ag | Verfahren zur Kontrolle eines zellulären Prozesses in Pflanzen |
| EP1639114A2 (en) * | 2003-07-01 | 2006-03-29 | Biolex, Inc. | Chloroplast transformation of duckweed |
| CN1687427A (zh) * | 2005-03-30 | 2005-10-26 | 湖南大学 | 基于超声波介导的纳米颗粒作载体的基因转导方法 |
| JP2008545621A (ja) * | 2005-04-20 | 2008-12-18 | デンドリティック ナノテクノロジーズ，インコーポレイテッド | 強化された拡大性と内部官能基性をもった樹枝状ポリマー |
| US20070016985A1 (en) * | 2005-07-18 | 2007-01-18 | Pioneer Hi-Bred International, Inc. | Particle Preparation for Direct-Delivery Transformation |
| AU2006288048A1 (en) | 2005-09-08 | 2007-03-15 | Biterials Co., Ltd. | Magnetic nanoparticle having fluorescent and preparation method thereof and use thereof |
| KR100821192B1 (ko) * | 2005-09-08 | 2008-04-11 | 주식회사바이테리얼즈 | 형광성을 가지는 자성 나노 입자 및 그 제조방법 |
| WO2007050715A2 (en) * | 2005-10-26 | 2007-05-03 | Integrated Plant Genetics, Inc. | Compositions and methods for safe delivery of biologically-active plant transformation agents using non-fibrous silicon carbide powder |
| EP2007435B1 (en) | 2006-03-31 | 2019-12-18 | Massachusetts Institute Of Technology | System for targeted delivery of therapeutic agents |
| PL2049663T3 (pl) * | 2006-08-11 | 2015-08-31 | Dow Agrosciences Llc | Homologiczna rekombinacja za pośrednictwem nukleazy z palcami cynkowymi |
| CN101889090B (zh) | 2007-10-05 | 2018-01-23 | 陶氏益农公司 | 用于将分子物质转移入植物细胞中的方法 |
-
2010
- 2010-04-07 TW TW099110728A patent/TWI576432B/zh active
- 2010-04-07 KR KR1020117026339A patent/KR101701534B1/ko active IP Right Grant
- 2010-04-07 AR ARP100101171A patent/AR076225A1/es active IP Right Grant
- 2010-04-07 SI SI201030952T patent/SI2417262T1/sl unknown
- 2010-04-07 EP EP10714403.2A patent/EP2417262B1/en active Active
- 2010-04-07 RU RU2015120061A patent/RU2612156C2/ru active
- 2010-04-07 DK DK10714403.2T patent/DK2417262T3/da active
- 2010-04-07 ES ES10714403.2T patent/ES2539327T3/es active Active
- 2010-04-07 HU HUE10714403A patent/HUE026053T2/en unknown
- 2010-04-07 JP JP2012504794A patent/JP5755636B2/ja active Active
- 2010-04-07 CN CN201080015582.0A patent/CN102369287B/zh active Active
- 2010-04-07 WO PCT/US2010/030155 patent/WO2010118077A1/en active Application Filing
- 2010-04-07 BR BRPI1015952A patent/BRPI1015952B8/pt active IP Right Grant
- 2010-04-07 AU AU2010234539A patent/AU2010234539B2/en active Active
- 2010-04-07 PT PT107144032T patent/PT2417262E/pt unknown
- 2010-04-07 RU RU2011144872/10A patent/RU2556376C2/ru active
- 2010-04-07 US US12/755,447 patent/US9187755B2/en active Active
- 2010-04-07 CA CA2757831A patent/CA2757831C/en active Active
-
2011
- 2011-08-25 ZA ZA2011/06262A patent/ZA201106262B/en unknown
- 2011-08-29 IL IL214880A patent/IL214880A/en active IP Right Grant
-
2015
- 2015-05-27 JP JP2015107100A patent/JP6275668B2/ja active Active
- 2015-05-27 HR HRP20150564TT patent/HRP20150564T1/hr unknown
- 2015-09-22 US US14/861,864 patent/US9719108B2/en active Active
-
2017
- 2017-02-14 RU RU2017104660A patent/RU2664865C2/ru active
- 2017-05-17 JP JP2017098360A patent/JP6513127B2/ja active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HRP20150564T1 (hr) | Nanoäśesticama posredovana sekvenca specifiäśnih nukleaza | |
| RU2010112423A (ru) | Способы переноса молекулярных веществ в клетки растений | |
| RU2012120081A (ru) | Применение дендримерной нанотехнологии для доставки биомолекул в растительные клетки | |
| Froelich et al. | Phloem ultrastructure and pressure flow: sieve-element-occlusion-related agglomerations do not affect translocation | |
| JP2021180667A (ja) | 植物における安定な形質転換のためのｐｅｇ化量子ドットを用いた直鎖ｄｎａ分子送達 | |
| RU2013147198A (ru) | Конъюгаты квантовой точки с пептидом-носителем, пригодные для визуализации и доставки молекул в растения | |
| JP6418738B2 (ja) | 官能化直鎖ｄｎａカセットの製造および植物における量子ドット／ナノ粒子媒介送達 | |
| RU2012146320A (ru) | Растительный пептид гамма-зеин для доставки биомолекул в растительные клетки | |
| Gulzar et al. | Plant tissue culture: agriculture and industrial applications | |
| Zhi et al. | The promising nanovectors for gene delivery in plant genome engineering | |
| Xia et al. | From mouse to mouse‐ear cress: Nanomaterials as vehicles in plant biotechnology | |
| De Filippis | Crop improvement through tissue culture | |
| Shaw et al. | Visualisation of stromules in transgenic wheat expressing a plastid-targeted yellow fluorescent protein | |
| Dayang Izawati et al. | Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens | |
| JP6815322B2 (ja) | 色素体形質転換方法 | |
| Chen et al. | Genetic modification of soybean with a novel grafting technique: downregulating the FAD2-1 gene increases oleic acid content | |
| Kausch et al. | Maize tissue culture, transformation, and genome editing | |
| Sheikh Mohamed et al. | Application of nanotechnology in genetic improvement in crops | |
| Begum et al. | A review of nanotechnology as a novel method of gene transfer in plants | |
| Ghosh | Current approaches and future potential for delivering CRISPR/Cas components in oilseeds and millets | |
| Yong et al. | Enhancing plant biotechnology by nanoparticle delivery of nucleic acids | |
| Tian et al. | Establishment of protoplasts transient expression system in Pinellia ternata (Thunb.) Breit | |
| Ariyarathne | Unraveling the Regulatory Basis of the Desiccation Tolerance Trait in Selaginella lepidophylla | |
| Rice | Optimisation of Carbon Dot-facilitated Wheat Transformation | |
| NZ616041B2 (en) | Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants |