RU2011144872A - Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз - Google Patents
Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз Download PDFInfo
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Abstract
1. Способ введения сиквенс-специфичной нуклеазы (ССН) в растительную клетку, где способ включаетобеспечение наличия растительной клетки, имеющей клеточную стенку;покрытие наночастицы ССН;приведение растительной клетки, имеющей клеточную стенку, и покрытой наночастицы в контакт друг с другом; иобеспечение поглощения наночастицы и ССН растительной клеткой, имеющей клеточную стенку.2. Способ по п.1, в котором покрытие наночастицы ССН включает иммобилизацию ССН на поверхности наночастицы за счет нековалентной адсорбции.3. Способ по п.1, который дополнительно включает поглощение ССН внутрь наночастицы.4. Способ по п.1, который дополнительно включает обеспечение поглощения наночастицы компартментом растительной клетки, имеющей клеточную стенку.5. Способ по п.4, который дополнительно включает покрытие наночастицы пептидом, проникающим в клетку, или белком, обеспечивающим направленную доставку в субклеточный компартмент.6. Способ по п.4, в котором компартмент выбран из группы, состоящей из цитозоля, ядра, тонопластов, пластиды, этиопласта, хромопласта, лейкопласта, элайопласта, протеинопласта, амилопласта, хлоропласта и полости двухслойной мембраны.7. Способ по п.1, в котором растительная клетка, имеющая клеточную стенку, выбрана из группы, состоящей из клеток табака, моркови, кукурузы, канолы, рапса, хлопчатника, пальмы, арахиса, сои,,,и сахарного тростника.8. Способ по п.1, в котором растительная клетка является клеткой ткани, выбранной из группы, состоящей из зародыша, меристемы, каллуса, пыльцы, листьев, пыльников, корней, корневых кончиков, цветков, семян, стручков и стеблей.9. Способ по п.1, в котором наночастица выбрана и�
Claims (16)
1. Способ введения сиквенс-специфичной нуклеазы (ССН) в растительную клетку, где способ включает
обеспечение наличия растительной клетки, имеющей клеточную стенку;
покрытие наночастицы ССН;
приведение растительной клетки, имеющей клеточную стенку, и покрытой наночастицы в контакт друг с другом; и
обеспечение поглощения наночастицы и ССН растительной клеткой, имеющей клеточную стенку.
2. Способ по п.1, в котором покрытие наночастицы ССН включает иммобилизацию ССН на поверхности наночастицы за счет нековалентной адсорбции.
3. Способ по п.1, который дополнительно включает поглощение ССН внутрь наночастицы.
4. Способ по п.1, который дополнительно включает обеспечение поглощения наночастицы компартментом растительной клетки, имеющей клеточную стенку.
5. Способ по п.4, который дополнительно включает покрытие наночастицы пептидом, проникающим в клетку, или белком, обеспечивающим направленную доставку в субклеточный компартмент.
6. Способ по п.4, в котором компартмент выбран из группы, состоящей из цитозоля, ядра, тонопластов, пластиды, этиопласта, хромопласта, лейкопласта, элайопласта, протеинопласта, амилопласта, хлоропласта и полости двухслойной мембраны.
7. Способ по п.1, в котором растительная клетка, имеющая клеточную стенку, выбрана из группы, состоящей из клеток табака, моркови, кукурузы, канолы, рапса, хлопчатника, пальмы, арахиса, сои, Oryza sp., Arabidopsis sp., Ricinus sp. и сахарного тростника.
8. Способ по п.1, в котором растительная клетка является клеткой ткани, выбранной из группы, состоящей из зародыша, меристемы, каллуса, пыльцы, листьев, пыльников, корней, корневых кончиков, цветков, семян, стручков и стеблей.
9. Способ по п.1, в котором наночастица выбрана из группы, состоящей из наночастиц золота, наночастиц, покрытых золотом, пористых наночастиц, мезопористых наночастиц, наночастиц диоксида кремния, наночастиц полимера, наночастиц вольфрама, наночастиц желатина, нанооболочек, наноядер, наносфер, наностержней, магнитных наночастиц, наночастиц полупроводников, квантовых точек, наноматриц, дендримерных наноматриц и их комбинаций.
10. Способ по п.1, который дополнительно включает в себя модификацию поверхности наночастицы.
11. Способ по п.1, в котором ССН является нуклеазой с цинковым пальцем, включающей белок с цинковым пальцем с неспецифичным в отношении последовательности доменом нуклеазы.
12. Способ по п.11, в котором неспецифичный в отношении последовательности домен нуклеазы получен из эндонуклеазы рестрикции FokI типа IIS.
13. Способ по п.11, который дополнительно включает отбор клеток, имеющих стабильно интегрированную ZFN.
14. Способ по п.13, в котором отобранные клетки являются регенерируемыми клетками.
15. Способ по п.14, который дополнительно включает регенерацию растения из регенерируемых клеток.
16. Способ по п.1, в котором наночастица является мультифункционализированной наночастицей.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16738909P | 2009-04-07 | 2009-04-07 | |
| US61/167,389 | 2009-04-07 | ||
| PCT/US2010/030155 WO2010118077A1 (en) | 2009-04-07 | 2010-04-07 | Nanoparticle mediated delivery of sequence specific nucleases |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015120061A Division RU2612156C2 (ru) | 2009-04-07 | 2010-04-07 | Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз |
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| Publication Number | Publication Date |
|---|---|
| RU2011144872A true RU2011144872A (ru) | 2013-05-20 |
| RU2556376C2 RU2556376C2 (ru) | 2015-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2015120061A RU2612156C2 (ru) | 2009-04-07 | 2010-04-07 | Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз |
| RU2011144872/10A RU2556376C2 (ru) | 2009-04-07 | 2010-04-07 | Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз |
| RU2017104660A RU2664865C2 (ru) | 2009-04-07 | 2017-02-14 | Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз |
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| RU2015120061A RU2612156C2 (ru) | 2009-04-07 | 2010-04-07 | Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз |
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| RU2017104660A RU2664865C2 (ru) | 2009-04-07 | 2017-02-14 | Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз |
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| Country | Link |
|---|---|
| US (2) | US9187755B2 (ru) |
| EP (1) | EP2417262B1 (ru) |
| JP (3) | JP5755636B2 (ru) |
| KR (1) | KR101701534B1 (ru) |
| CN (1) | CN102369287B (ru) |
| AR (1) | AR076225A1 (ru) |
| AU (1) | AU2010234539B2 (ru) |
| BR (1) | BRPI1015952B8 (ru) |
| CA (1) | CA2757831C (ru) |
| DK (1) | DK2417262T3 (ru) |
| ES (1) | ES2539327T3 (ru) |
| HR (1) | HRP20150564T1 (ru) |
| HU (1) | HUE026053T2 (ru) |
| IL (1) | IL214880A (ru) |
| PT (1) | PT2417262E (ru) |
| RU (3) | RU2612156C2 (ru) |
| SI (1) | SI2417262T1 (ru) |
| TW (1) | TWI576432B (ru) |
| WO (1) | WO2010118077A1 (ru) |
| ZA (1) | ZA201106262B (ru) |
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| US20170000743A1 (en) * | 2015-07-02 | 2017-01-05 | Vindico NanoBio Technology Inc. | Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles |
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| US11213594B2 (en) | 2016-04-29 | 2022-01-04 | Poseida Therapeutics, Inc. | Poly(histidine)-based micelles for complexation and delivery of proteins and nucleic acids |
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