NZ616041B2 - Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants - Google Patents

Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants Download PDF

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Publication number
NZ616041B2
NZ616041B2 NZ616041A NZ61604112A NZ616041B2 NZ 616041 B2 NZ616041 B2 NZ 616041B2 NZ 616041 A NZ616041 A NZ 616041A NZ 61604112 A NZ61604112 A NZ 61604112A NZ 616041 B2 NZ616041 B2 NZ 616041B2
Authority
NZ
New Zealand
Prior art keywords
cell
nucleic acids
interest
plant
gene
Prior art date
Application number
NZ616041A
Other versions
NZ616041A (en
Inventor
Frank G Burroughs
Gaofeng Lin
Narasimha Chary Samboju
Jayakumar Pon Samuel
Steven R Webb
Kerrm Y Yau
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Priority claimed from PCT/US2012/030195 external-priority patent/WO2012129443A2/en
Publication of NZ616041A publication Critical patent/NZ616041A/en
Publication of NZ616041B2 publication Critical patent/NZ616041B2/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

method of introducing one or more nucleic acids of interest into a plant cell having a cell wall to effect stable transformation of a plant and seeds is disclosed. The method comprises providing the plant cell having a cell wall; interacting a quantum dot (semi-conductor nanoparticle) with one or more cell penetrating peptides to form a quantum dot-peptide conjugate; attaching the one or more nucleic acids of interest to the one or more cell penetrating peptides to form an activated quantum dot-peptide conjugate; placing the cell having a cell wall and the activated quantum dot-peptide conjugate in contact with each other; and allowing uptake of the quantum dot-peptide conjugate and the one or more nucleic acids of interest into the cell having the cell wall. r more cell penetrating peptides to form a quantum dot-peptide conjugate; attaching the one or more nucleic acids of interest to the one or more cell penetrating peptides to form an activated quantum dot-peptide conjugate; placing the cell having a cell wall and the activated quantum dot-peptide conjugate in contact with each other; and allowing uptake of the quantum dot-peptide conjugate and the one or more nucleic acids of interest into the cell having the cell wall.

Description

PCT/U82012/030195 QUANTUM DOT CARRIER PEPTIDE CONJUGATES SUITABLE FOR IMAGING AND DELIVERY APPLICATIONS IN PLANTS TY

Claims (20)

CLAIM What may be d is:
1. A method of introducing one or more nucleic acids of st into a plant cell having a cell wall to effect stable transformation of a plant and seeds, the method comprising: 5 providing the plant cell having a cell wall; cting a quantum dot (QD) with one or more cell penetrating es (CPPs) to form a QD-peptide conjugate; attaching the one or more nucleic acids of interest to the one or more CPPs to form an activated QD-peptide conjugate; 1O placing the cell having a cell wall and the activated QD—peptide conjugate in contact 1 with each other; and allowing uptake of the QD-peptide conjugate and the one or more nucleic acids of interest into the cell having the cell wall.
2. The method according to claim 1, wherein interacting a QB with one or more CPPs 15 comprises assembly of the one or more CPPs onto the surface of the QD.
3. The method according to claim 1 or claim 2, wherein attaching the one or more nucleic acids of interest to the one or more CPPS comprises cting negatively charged groups of the nucleic acids of interest with positively charged amino groups at a C-terminal end of the CPPs.
4. The method according to any one of the preceding claims, further comprising ng (( 20 uptake of the activated QD-peptide conjugate into a compartment of the plant cell sing a cell wall.
5. The method ing to claim 4, wherein the compartment is selected from the group consisting of cytosol, nucleus, tonoplasts, plastid, etioplast, chromcplast, leucoplast, elaioplast, proteinoplast, amyloplast, chloroplast, and the lumen of the double membrane. 25
6. The method according to any one of the preceding claims, wherein the plant cell comprising a cell wall is selected from the group consisting of tobacco, carrot, maize, canola, ed, cotton, palm. peanut, soybean, Org/2a Sp, Arabidopsz's Sp.. Ricinus Sp., and sugarcane cells. 23m 21':b - 4O -
7. The method according to any one of the preceding claims, n the plant cell is from a tissue selected from the group consisting of embryo, ematic, callus, pollen, leaves, anthers, roots, root tips, flowers, seeds, pods and stems.
8. The method according to any one of the preceding claims, wherein the one or more 5 CPPs is ed from the group consisting of R9, MPG, TAT, y-Zein, and PEPl peptides.
9. The method according to any one of the preceding claims, n the one or more nucleic acids of st comprises a component selected from the group ting of DNA, RNA, RNAi molecules, genes, plasmids, cosmids, YACs, BACs, and combinations thereof.
10. The method according to claim 9, wherein the one or more nucleic acids of interest i 10 comprises a gene.
11. The method according to claim 10, wherein the gene is a foreign protein gene, an agronomic gene, or a marker gene.
12. The method according to any one of the preceding claims, further comprising selecting cells that have stably ated the one or more nucleic acids of interest. 15
13. The method according to claim 12, wherein the selected cells are regenerable cells.
14. The method according to claim 13, further comprising regenerating a plant from the regenerable cells.
15. The method according to claim 1, wherein the one or more nucleic acids of interest is gene, wherein the gene is stably expressed in the plant cell or expressed in progeny of a plant 20 having the plant cell.
16. The method according to claim 15, wherein the gene is expressed in a chloroplast.
17. The method ing to claim 15 or claim 16, further comprising ing for cells stably expressing the gene.
18. The method according to any one of claims 1—9, and 12—14, wherein the one or more 25 nucleic acids of interest is plasmid DNA.
19. The method of claim 18, further comprising stably expressing one or more genes from the plasmid DNA in progeny of a plant having the plant cell. IOSZ ‘41 ' 23~l~LAR US
20. A method according to any one of the preceding claims further comprising imaging the plant cell having the cell wall for screening and identifying plant transformation. W0
NZ616041A 2011-03-23 2012-03-22 Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants NZ616041B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161466804P 2011-03-23 2011-03-23
US61/466,804 2011-03-23
PCT/US2012/030195 WO2012129443A2 (en) 2011-03-23 2012-03-22 Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants

Publications (2)

Publication Number Publication Date
NZ616041A NZ616041A (en) 2015-04-24
NZ616041B2 true NZ616041B2 (en) 2015-07-28

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