BRPI0822775A2 - Método de obtenção de uma proteína heteróloga, purificada, biologicamente ativa. - Google Patents

Método de obtenção de uma proteína heteróloga, purificada, biologicamente ativa.

Info

Publication number
BRPI0822775A2
BRPI0822775A2 BRPI0822775-6A BRPI0822775A BRPI0822775A2 BR PI0822775 A2 BRPI0822775 A2 BR PI0822775A2 BR PI0822775 A BRPI0822775 A BR PI0822775A BR PI0822775 A2 BRPI0822775 A2 BR PI0822775A2
Authority
BR
Brazil
Prior art keywords
obtaining
biologically active
heterologous protein
purified biologically
active heterologous
Prior art date
Application number
BRPI0822775-6A
Other languages
English (en)
Inventor
Partha Hazra
Nitesh Dave
Vivekanandan Kannan
Sanjay Tiwari
Anuj Goel
Harish Iyer
Nita Roy
Krishnamurthy Venkatesan
Anoop Vasudevan
Anupama Jagadish
Goldy Sachdev
Mukesh Babuappa Patale
Original Assignee
Biocon Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocon Ltd filed Critical Biocon Ltd
Publication of BRPI0822775A2 publication Critical patent/BRPI0822775A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BRPI0822775-6A 2008-02-19 2008-04-03 Método de obtenção de uma proteína heteróloga, purificada, biologicamente ativa. BRPI0822775A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN420CH2008 2008-02-19
PCT/IN2008/000219 WO2009104199A1 (en) 2008-02-19 2008-04-03 A method of obtaining purified heterologous insulins expressed in yeast

Publications (1)

Publication Number Publication Date
BRPI0822775A2 true BRPI0822775A2 (pt) 2015-06-30

Family

ID=40985112

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0822775-6A BRPI0822775A2 (pt) 2008-02-19 2008-04-03 Método de obtenção de uma proteína heteróloga, purificada, biologicamente ativa.

Country Status (11)

Country Link
US (1) US8802816B2 (pt)
EP (1) EP2242767A4 (pt)
JP (2) JP5781308B2 (pt)
KR (1) KR101556514B1 (pt)
CN (1) CN102015762B (pt)
BR (1) BRPI0822775A2 (pt)
IL (1) IL207646A (pt)
MX (1) MX2010009033A (pt)
MY (1) MY161892A (pt)
RU (1) RU2495131C2 (pt)
WO (1) WO2009104199A1 (pt)

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EP2408470A4 (en) 2009-03-20 2012-08-29 Smartcells Inc SOLUBLE NONDEPOT INSULIN CONJUGATE AND ITS USE
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KR101836070B1 (ko) 2009-11-13 2018-03-09 사노피-아벤티스 도이칠란트 게엠베하 Ｇｌｐ－１ 작용제, 인슐린 및 메티오닌을 포함하는 약제학적 조성물
JP5973918B2 (ja) 2009-11-13 2016-08-23 サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Ｇｌｐ−１アゴニスト及びメチオニンを含む薬学的組成物
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CN102219851B (zh) * 2011-05-09 2012-05-30 甘李药业有限公司 甘精胰岛素结晶的制备方法
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
CN108079281A (zh) 2011-08-29 2018-05-29 赛诺菲-安万特德国有限公司 用于2型糖尿病患者中的血糖控制的药物组合
AR087744A1 (es) 2011-09-01 2014-04-16 Sanofi Aventis Deutschland Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
WO2013144685A1 (en) 2012-03-29 2013-10-03 Biocon Limited Secretion of functional insulin glargine directly into the culture medium through over expression of kex2p intracellularly
WO2013164375A1 (en) * 2012-05-01 2013-11-07 Novo Nordisk A/S Pharmaceutical composition
CN103833828B (zh) * 2012-11-21 2017-01-11 广东东阳光药业有限公司 一种甘精胰岛素前体蛋白的提取方法
EP2925345B1 (en) 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
CN102994600A (zh) * 2012-12-11 2013-03-27 鲁南新时代生物技术有限公司 甘精胰岛素前体的酶切转化方法
EP3517122A1 (en) 2013-04-03 2019-07-31 Sanofi Treatment of diabetes mellitus by long-acting formulations of insulins
MX366852B (es) 2013-10-04 2019-07-25 Merck Sharp & Dohme Conjugados de insulina sensibles a glucosa.
FR3013049B1 (fr) 2013-11-14 2015-11-13 You-Ping Chan Analogue de l'insuline glargine
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
CN107206058A (zh) 2014-12-12 2017-09-26 赛诺菲-安万特德国有限公司 甘精胰岛素/利西拉来固定比率配制剂
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
CN106117345B (zh) * 2015-05-05 2020-11-24 宜昌东阳光长江药业股份有限公司 一种制备甘精胰岛素结晶的方法
CN106282274B (zh) * 2015-06-29 2021-05-11 宜昌东阳光长江药业股份有限公司 一种胰岛素前体蛋白的毕赤酵母高密度发酵方法
JP2019501982A (ja) * 2015-10-30 2019-01-24 積水化学工業株式会社 熱伝導体、ヒートシンク、ヒートスプレッダ、ヒートスプレッダの製造方法、及び熱伝導体の製造方法
JP7189767B2 (ja) 2015-11-19 2022-12-14 武田薬品工業株式会社 組換えヒトｃ１エステラーゼインヒビター及びその使用
RU2736358C2 (ru) * 2015-12-09 2020-11-16 Общество С Ограниченной Ответственностью "Синбио" Способ очистки полисиалированного инсулина человека
PL239062B1 (pl) * 2016-01-22 2021-11-02 Inst Biotechnologii I Antybiotykow Sposób wytwarzania insuliny i jej pochodnych
CN105585628B (zh) * 2016-01-28 2019-02-01 通化东宝药业股份有限公司 一种甘精胰岛素的制备方法及其制备的甘精胰岛素
CN108148114B (zh) * 2017-12-08 2020-05-15 珠海冀百康生物科技有限公司 一种提高重组小分子蛋白表达量的串联前导肽及方法
CN108047301A (zh) * 2017-12-08 2018-05-18 福州博中技术开发有限公司 一种纯化有机酸肽的方法
JP2021532730A (ja) * 2018-06-18 2021-12-02 ユニケム ラボラトリーズ リミテッド 組換えタンパク質の発現レベルを高めるためのリーダー配列
KR102666154B1 (ko) 2018-08-08 2024-05-20 주식회사 대웅제약 지속형 인슐린 아날로그 및 그 복합체
KR102646845B1 (ko) 2018-08-08 2024-03-14 주식회사 대웅제약 클로스트리파인을 이용한 지속형 인슐린 아날로그 복합체의 활성형 제조방법
JP7457023B2 (ja) * 2018-08-20 2024-03-27 バイオコン バイオロジクス リミテッド インスリンアナログの精製のためのクロマトグラフィープロセス
CN109735589B (zh) * 2019-01-02 2020-07-10 珠海冀百康生物科技有限公司 一种胰岛素DesB30的制备方法
WO2020144606A1 (en) * 2019-01-10 2020-07-16 Biocon Limited Preparative crystallization of recombinant human insulin
CN112341535A (zh) * 2019-08-07 2021-02-09 中国科学院大连化学物理研究所 一种采用离子交换色谱法分离纯化制备胰岛素的方法
CN113121637B (zh) * 2020-01-15 2022-06-14 鲁南制药集团股份有限公司 一种重组蛋白的分离纯化方法
CN113773399B (zh) * 2020-06-09 2023-05-12 宁波鲲鹏生物科技有限公司 一种甘精胰岛素衍生物及其应用
CN113105536B (zh) * 2020-09-11 2023-07-18 美药星（南京）制药有限公司 一种新甘精胰岛素原及其制备甘精胰岛素的方法
CN114805610B (zh) * 2022-06-23 2022-10-04 北京惠之衡生物科技有限公司 一种高表达甘精胰岛素前体的重组基因工程菌及其构建方法

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Also Published As

Publication number Publication date
RU2495131C2 (ru) 2013-10-10
IL207646A (en) 2016-08-31
JP2015083019A (ja) 2015-04-30
JP2011514151A (ja) 2011-05-06
IL207646A0 (en) 2010-12-30
RU2010138656A (ru) 2012-03-27
MY161892A (en) 2017-05-15
JP6077027B2 (ja) 2017-02-08
CN102015762A (zh) 2011-04-13
EP2242767A1 (en) 2010-10-27
CN102015762B (zh) 2015-03-18
US20110236925A1 (en) 2011-09-29
US8802816B2 (en) 2014-08-12
KR101556514B1 (ko) 2015-10-02
JP5781308B2 (ja) 2015-09-16
MX2010009033A (es) 2010-12-21
EP2242767A4 (en) 2011-04-06
KR20100117612A (ko) 2010-11-03
WO2009104199A1 (en) 2009-08-27

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08H Application fees: decision cancelled [chapter 8.8 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 NA RPI 2336 DE 13/10/2015

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]